Regulatory T Cells and Immunotherapy for Gliomas

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ISSN 1007-7626 CN 11-3870 / Q http / /cjbmb bjmu edu cn Chinese Journal of Biochemistry and Molecular Biology 2015 2 31 2 135 ~ 139 DOI 10 13865 /j cnki cjbmb 2015 02 04 T * 410000 T regulatory T cell CD 4 + T CD 8 + T NK B T R73 Regulatory T Cells and Immunotherapy for Gliomas FENG Jian-Bo WU Ming-Hua * LI Gui-Yuan Cancer Research Institute of Central South University Changsha 410000 China Abstract Regulatory T cell is a cell type that negatively regulates other immune cells suppresses the activation and proliferation of CD 4 + and CD 8 + lymphocytes NK cells B lymphocytes and dendritic cells to keep the stability of immune system and to avoid the occurrence of autoimmune diseases In recent research cells was known to a play critical role in tumors as tumor cells can suppress immune response by propagating or recruiting cells This suggested that the role of may be significant in tumorigenesis and progression Therefore reducing the number or activation of cells could be an effective way to improve the immunotherapy of tumors including glioma Key words regulatory T cell immune escape glioma immunotherapy T CD 4 + CD 25 + FoxP3 + 3 20 70 T 1995 Sakaguchi 1 CD 4 + CD 25 + T CD 4 CD 25 CD 4 + CD 25 + T 1 1 2014-08-15 2014-09-05 No 81272297 2 1 * Tel 0731-82355401 E-mail wuminghua554@ aliyun com Received August 15 2014 Accepted September 5 2014 Supported by National Natural Science Foundation of China No 81272297 * Corresponding author Tel 0731-82355401 E-mail wuminghua554@ aliyun com

136 31 CD4 + CD8 + NK CD4 + CD25 + 1 1 1 CD4 + CD 4 + thymus-derived regulatory T cell t CD 122 + CD 8 + peripherally derived regulatory T cell pcd 8 + CD 8 + CD 28- CD8 + in vitro-induced regulatory T cell i 4 T CD4 + CD8 + CD 4 + Foxp3 T Foxp3 5 MS CD 4 + CD 25 + CD 4 + CD 25 + CD 4 + CD 25 + CD4 + T 5% ~ 10% T T CD 8 + IL-2 /IFNγ 6 CD 4 + CD 25 + CD4 2 α CD25 CD8 + CD8 + T 4 cytotoxic T lymphocyteassociated antigen-4 CTLA-4 T 5% ~ 10% glucocorticoid-induced tumor necrosis factor receptor GITR CD 4 + CD 25 + 11 NK 2 Foxp3 T CD56 bright CD16 - NK CD56 dim CD16 + NK CD56 dim CD16 + NK 90% Foxp3 T 10% CD56 bright CD16 - CD127 Foxp3 + CD127 NK CD 4 + CD 25 + CD127 Tian CD 4 + CD 25 + CD127 12 NK CD 4 + CD 25 + Verneris 13 NK Foxp3 7 CD 4 + CD 25 + NK T 2 IL-2 NK T T cell antigen receptor TCR NK B 8 9 1 1 2 CD8 + CD 8 + CD 8 + CD 8 + CD 8 + CD 8 + CD 25 + CD 8 + Foxp3 + Kouchaki 10 CD8 + CD8 + IL-6 IL-10 CD8 + 1 1 3 NK NK T CD3 - CD56 + CD3 - CD56 + allo-hct CD 4 + CD 8 + T NK 1 2 CD4 + CD8 + T B APC antigen presenting cell CD 4 + CD 25 + CD8 + T

2 T 137 1 2 14 2 1 2 1 3 - major CTLA-4 histocompatibility complex MHC IL-2 T CTLA-4 CTLA-4 2 2 15 1 2 2 TGF-β IL-10 1 MHC TGF-β B7H1 TGF-β B7-H1 i 1 programmed death-1 TGF-β PD-1 T 17 2 NK Ig IFN-γ IL-2 B IL-10 3 16 IL-10 cyclooxygenase COX A E2 prostaglandin E2 PGE2 1 3 PGE2 inductible nitric oxide synthase inos vascular endothelial growth factor VEGF T 18 DC IL-2 IFN-α PGE2 COX-2-1 inhibitor of differentiation-1 ID1 19 4 T T 3 CD 4 + CD 25 + Foxp3 T 2 Ⅱ- 2 1 FasL TGF-β PGE Ⅲ-Ⅳ 20 Wainwright 21

138 31 natural regulatory T cell n induced regulatory T cell i References n n n i n i n i CD 4 + T i n n i Neuropilin-1 Helios n i n i foxp3 + CCL2 CC chemokine ligand-2 730380 74% CCL2 CCR4 CC chemokine receptor 4 34% 22 CCL2 CCR4 23 4 1 Sakaguchi S Sakaguchi N Asano M et al Immunologic selftolerance maintained by activated T cells expressing IL-2 receptor α-chains CD25 multiple sclerosis J 255 with interferon-α J autoimmune disease J Hematol Educ Program 14 J Breakdown of a single mechanism of selftolerance causes various autoimmune diseases J J Immunol 1995 155 3 1151-1164 2 Curiel T J Regulatory T cells and treatment of cancer J Curr Opin Immunol 2008 20 2 241-246 3 Wainwright D A Dey M Chang A et al Targeting s in malignant brain cancer overcoming IDO J Front Immunol 2013 4 116 4 Abbas A K Benoist C Bluestone J A et al Regulatory T cells recommendations to simplify the nomenclature J Nat Immunol 2013 14 4 307-308 5 Curotto de Lafaille M A Lafaille J J Natural and adaptive regulatory T cells more of the same or a division of labor J Immunity 2009 30 5 626-635 6 Duan M C Zhong X N Liu G N et al The /Th17 paradigm in lung cancer J J Immunol Res 2014 2014 7 Liu W Putnam A L Zhou X Y et al CD127 expression inversely correlates with FoxP3 and suppressive function of human CD 4 + cells J J Exp Med 2006 203 7 1701-1711 8 Zhao E Wang L Dai J et al Regulatory T cells in the bone marrow microenvironment in patients with prostate cancer J Oncoimmunology 2012 1 2 152-161 9 Ganesan A P Johansson M Ruffell B et al Tumor-infiltrating regulatory T cells inhibit endogenous cytotoxic T cell responses to lung adenocarcinoma J J Immunol 2013 191 4 2009-2017 10 Kouchaki E Salehi M Reza Sharif M et al Numerical status of CD4 + CD25 + FoxP3 + and CD8 + CD28 - regulatory T cells in Iran J Basic Med Sci 2014 17 4 250-11 Riley C H Hansen M Brimnes M K et al Expansion of circulating CD56 bright natural killer cells in patients with JAK2- positive chronic myeloproliferative neoplasms during treatment Eur J Haematol 2014 doi 10 1111 / ejh 12420 Epub ahead of print 12 Tian Z Gershwin M E Zhang C Regulatory NK cells in J Autoimmun 2012 39 3 206-215 13 Verneris M R Natural killer cells and regulatory T cells how to manipulate a graft for optimal GVL J Hematology Am Soc 2013 2013 335-341 T Fan M Huo J G Advancement in the research on regulatory T cell in tumor immune escape and China traditional medicine treatment J J

2 T 139 Zhejiang Univ Tradit Chin Med 2013 37 6 826-829 15 Cribbs A P Kennedy A Penn H et al Regulatory T cell function in rheumatoid arthritis is compromised by CTLA-4 promoter methylation resulting in a failure to activate the IDO pathway J Arthritis Rheumatol 2014 66 9 2344-2354 16 Kim S Buchlis G Fridlender Z G et al Systemic blockade of transforming growth factor-beta signaling augments the efficacy of immunogene therapy J Cancer Res 2008 68 24 10247-10256 17 Yao Y Tao R Wang X et al B7-H1 is correlated with malignancy-grade gliomas but is not expressed exclusively on tumor stem-like cells J Neuro Oncol 2009 11 6 757-766 18 El-Sayed M Taha M M Immunohistochemical expression of cycloxygenase-2 in astrocytoma correlation with angiogenesis tumor progression and survival J Turk Neurosurg 2011 21 1 27-35 19 Xu K Wang L Shu H K COX-2 overexpression increases malignant potential of human glioma cells through Id1 J Oncotarget 2014 5 5 1241-1252 20 Heimberger A B Abou-Ghazal M Reina-Ortiz C et Incidence and prognostic impact of FoxP3 + regulatory T cells in human gliomas J Clin Cancer Res 2008 14 16 5166-5172 21 Wainwright D A Sengupta S Han Y et al Thymus-derived rather than tumor-induced regulatory T cells predominate in brain tumors J Neuro Oncol 2011 13 12 1308-1323 22 Jordan J T Sun W Hussain S F et al Preferential migration of regulatory T cells mediated by glioma-secreted chemokines can be blocked with chemotherapy J Cancer Immunol Immunother 2008 57 1 123-131 23 Jacobs J F Idema A J Bol K F et al Prognostic significance and mechanism of infiltration in human brain tumors J J Neuroimmunol 2010 225 1-2 195-199 al