2008, 35 (12) : 1837-1842 Acta Horticulturae Sinica SRAP 1, 1, 2, 2, 2, 13 ( 1, 100193; 2, 100102) : SRAP ( sequence2related amp lified polymorphism) 88 58, 20, 389, 1915, 13 40, Jaccardπs0155 0187 0167 584, 1, 2, 3 4, : ; SRAP; : S 681: A : 05132353X (2008) 1221837206 Genetic Rela tion sh ip of Som e Cultivars of Petun ia Hybr ids Using SRAP M arker XU J in 1, ZHANG Xi2xi 1, 2, DONG A i2xiang 2, WANG Tao 2, and ZHAO L iang2jun 13 ( 1 D epartm ent of O rnam ental Horticulture and Landscape A rchitecture, China A gricultural U niversity, B eijing 100193, China; 2 B eijing Institute of Horticulture and Gardening, B eijing 100102, China) Abstract: In this paper, the molecular m arker system 2SRAP ( sequence2related amp lified polymorphism) was firstly app lied on 58 petunia hybridsπgenetic relationship research. The results obtained was as follow s: 20 p rimer pairs selected from 88 p rimer pairs amp lified 389 polymorphic bands with an average of 1915 polymor2 phic bands per p rimer pair, and the number of bands amp lified by each p rimer pair was ranged from 13 to 40. Moreover, Jaccardπs sim ilarity coefficient ranged from 0155 to 0187. Fifty2eight cultivars could be divided into 4 group s when the genetic distance was 0167. The first group mainly included warm colour serieses, and yellow flower cultivars were in the second group, while cold colour cultivars were clustered in the third and fourth group. In addition, the white colour cultivars were clustered in a w ide range. Key words: Petun ia hybrida; SRAP; genetic relationship ( Petunia hybrida V ilm. ) 80,,, Cerny (1996) DAF (DNA2amp lification fingerp rinting) 5 10, DNA RAPD AFLP, RAPD, AFLP,, SRAP ( sequence2related am2 p lified polymorphism ) SRAP ORFs(open reading frames) 17 : 2008-06 - 06; : 2008-10 - 21 : (D070500340121) 3 Author for correspondence ( E2mail: zhaolj5073@1631com)
1838 35 bp, 5 10 bp, CCGG 3 3,, 18 bp, 5 11 bp, AATT 3 3, (L i & Quiros, 2001;, 2007), SRAP ( Ferriol et al., 2003, 2004;, 2005;, 2007;, 2007;, 2007), SRAP,, 1 111D NA 2007 2008, 58 ( 1) CTABDNA, DNA No. Cultivar 1 58 Table 1 Nam e and resource of 58 cultivars 3 Resource 3 No. Cultivar 1 D ream s Raki Red Lace a 30 Prism Red d 2 D ream s Trout Reda a 31 Hurrah B lue Stripe d 3 D ream s Red a 32 Hurrah Pink d 4 D ream s Rose Red Lace a 33 Hurrah Rose Red d 5 D ream s Rose Red a 34 Hurrah Pink Stripe d 6 D ream s Pink a 35 A laddin Red f 7 Primetime B lue b 36 A laddin Raki Red f 8 Primetime Red b 37 A laddin Rose Red f 9 Primetime Scarlet b 38 Frost Velvet f 10 Primetime W hite b 39 Frost Flam ing f 11 Primetime Pink b 40 Frost Cherry Red f 12 Primetime Rose Red b 41 B ravo Pink e 13 U ltra B lue Lace b 42 B ravo W hite e 14 U ltra Red b 43 B ravo B lue e 15 U ltra Pink b 44 Feng Hua Purp le g 16 M irage Sp ring a 45 Feng Hua W hite g 17 M irage Summer a 46 Feng Hua Rose Red g 18 M irage Purp le a 47 Merlin Red h 19 M irage B lue Lace a 48 Merlin Pink h 20 M irage Rakired Lace a 49 Merlin Cherry Red h 21 Symphonyrose Red c 50 Frost B lue b 22 Symphonyred Lace c 51 Frost Rose R ing b 23 Symphony Red c 52 Double Cascade Rose Red a 24 Symphonyrose Red Lace c 53 Double Cascade Red a 25 Symphony B lue c 54 Super Cascade Red a 26 Prism Rose Red d 55 Super Cascade B lue a 27 Prism W hite d 56 Jing Guan Rose Red i 28 Prism Yellow d 57 Jing Guan Purp le i 29 Prism B lue d 58 Jing Guan Red i 3 Resource 3 3 : a. ; b. ; c. ; d. ; e. ; f. ; g. ; h. ; i. 3 : a. Pan American; b. Goldsm ith; c. Takii; d. Floranova; e. Syngenta; f. Bodger; g. Chifeng Gold Horticulture; h. Sakata; i. Beijing Institute of Horticulture and Gardening.
12 : SRAP 1839 112 SRAP2PCR SRAP Ferriol (2003) Budak (2004), 8(ME) 11 ( EM ) 88 5 DNA, 88, 113 PCR PCRB IOMETRA Tgradient PCR () Ferriol ( 2003) 20 L : 1017 L, Buffer 3 L, dntps 2 L ( 215 mmol L - 1 ), 1 L (10 mol L - 1 ), DNA 2 L (20 ngl - 1 ), rtaq 013 L (01075 UL - 1 ) (2007) (6% ) 114 ( % ) = / 100 ( % ) = / 100 Jaccardπs, NTSYS2pc 210, (UPGMA) : S ij = a /( a + b + c), a, bi, cj 2 211 SRAP 88, 20 1 ME27 /EM 29 1 M E27 /EM 29 58() M. pbr322 marker; 1 581 F ig. 1 The am plifica tion result of 58 petun ia hybr ids by the pr im er pa ir M E27 /EM 29 M. DNA marker (pbr322) ; Cultivar number are the same as Table 1. A rrows show polymorphic bands.
1840 35 20 58 DNASRAP, ( 2), 13 40, 20527, 389, 7318%, 10 32, 1915, 6010% 8819% 2 20 Table 2 The num ber of polym orph ic bands genera ted by 20 pr im er pa irs Primer pair serial number Name of p rimer pair Total number Number of polymorphic bands /% Polymorphic rate Number of distinguishable kind /% D istinguished ability 1 ME21 / EM21 13 10 7619 14 2411 2 ME21 / EM22 24 19 7912 39 6712 3 ME21 / EM24 21 15 7114 47 8110 8 ME21 / EM28 33 26 7818 57 9813 9 ME21 / EM29 16 11 6818 41 7017 11 ME21 / EM211 19 13 6814 47 8110 12 ME22 / EM21 29 22 7519 41 7017 14 ME22 / EM23 21 14 6617 33 5619 16 ME22 / EM25 25 15 6010 31 5314 27 ME23 / EM25 35 25 7114 53 9114 36 ME24 / EM23 19 14 7317 47 8110 37 ME24 / EM24 23 15 6512 36 6210 59 ME26 / EM24 38 30 7819 50 8612 60 ME26 / EM25 36 32 8819 51 8719 65 ME26 / EM210 25 22 8810 54 9311 66 ME26 / EM211 23 20 8710 47 8110 68 ME27 / EM22 24 17 7018 38 6515 71 ME27 / EM25 24 15 6215 31 5314 75 ME27 / EM29 39 26 6617 58 100 77 ME27 / EM211 40 28 7010 54 9311 212 SRAP527, 58, Jaccardπs0155 0187, 0178 0167, 4 ( 2) 1, 45 ;, 2, 3 5 ; 8 4,, 1,,, 3 4
12 : SRAP 1841 F ig. 2 58 2 The dendrogram of phylogenetic rela tion sh ip of 58 cultivars of Petun ia hybrida 3 SRAP,,,, SRAP Cerny (1996) 5 10,,, 58, 1, 3 4,,,,,,
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