ISSN 100727626 CN 1123870gQ 16 3 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 3, 394 399 2000 6 3-1 2 3 3 3 (,, 100730) 21 2 3 (U CP1, 2, 3), R T 2PCR U CP 1, 2, 3 m RNA U CP 1. U CP 2, 3, U CP 2 ; U CP3 U CP1, 3 m RNA (P < 0101) ; U CP 2 m RNA, (P < 0105) ; U CP3 48 h, U CP 2, 3, U CP s U CP s, U CP s U CP3 (P < 0105) U CP s ; U CP s ; U CP s U CP s, 2,,,,, 2, R T 2PCR Q 51 Regula tory Effects of Fa sting and In sulin on Expression of Uncoupling Prote in-1, 2, 3 Genes J IN Jun2hua, ZHAN G T ie2m ei, ZHAN G En2yi,L I Y i (K ey L aboratory of Geriatrics, B eij ing Institute of Geriatrics, B eij ing H osp ital, M inistry of H ealth of P. R. China, B eij ing 100730, China) Abstract In o rder to investigate the effects of fasting (overn igh t and 48 hou rs) and in su lin on exp ression of uncoup ling p ro tein (U CP) 1, 2 and 3 genes, U CP1, 2 and 3 m RNA levels w ere ob2 served by reverse tran scrip tion g g po lym erase chain reaction (R T 2PCR ) assay in in terscapular b row n adipo se tissue (BA T ), periep ididym al w h ite adipo se tissue (W A T ) and gastrocnem iu s of rats. U CP 1 gene w as exp ressed exclu sively in BA T. U CP2 and 3 genes w ere exp ressed in these th ree types of tissues w hereas m RNA levels of U CP2 and U CP3 w ere rich in W A T and in skeletal m u scle respectively. U CP 1 and U CP 3 m RNA levels w ere decreased (P < 0101) in BA T bu t U CP 2 m RNA level w as increased in th ree types of tissues by overn igh t fasting. U CP3 gene exp ression w as no t influenced in W A T and skeletal m u scle by fasting. P ro longing fasting tim e to 48 hou rs, U CP s m RNA levels in all tissues w ere up regu lated to the sam e as o r over con tro l group excep t 3 (A ) 3 3 Tel: (010) 6513226622676, Fax: (010) 65237929, E2m ail: tm zhang@cenpok. net,, 1965 11, : 1999210229, : 2000202223
3 : 21 2 3 395 U CP2, 3 m RNA levels that w ere decreased in BA T. In su lin up regu lated U CP s gene exp ression in BA T,W A T and skeletalm u scle (P < 0105). U CP s gene exp ression of rats show ed tissue specifici2 ty. T he differen t exp ression of U CP s gene induced by fasting fim e suggests that the function of sub types of U CP s m ay p lay differen t ro les in energy m etabo lism regu lation. T hese resu lts have al2 so p rovided basis fo r fu rther understanding of the relation sh ip betw een diet2therm ogen sis and body w eigh t regu lation, and the association w ith disease and body w eigh t2m etabo lism equ ilib ri2 um. Key words U ncoup ling p ro tein, Fasting, In su lin,o besity, T ype 2 diadetes, R T 2PCR (uncoup ling p ro tein s, U CP s) U CP s,, 1, A T P,, 111 A T P,,U CP s 11111 : W istar 14 ( 8 U CP1, (BA T ), 11112 RNA TR Izo l [ 1 ] G IBCO, BA T P rom ega U CP2 112 U CP3, 11211 : (1) (n= U CP2 U CP3 3), ; (2) (n= 3),, U CP2 U CP3 5 8, ; (3) 73%, U CP1 59% 57%,U CP2 3 8, (1 IU g,,, ), 4 h ; (4) 48 h (n U CP2 3 U CP1 = 4), 8 2 d 8, [ 2, 3 ], (w h ite adipo se tissues, U CP s W A T ) (b row n adipo se tissues, BA T ) ( skeletal U CP2 m u scle, SM ) 100 m g, RNA, 2 [ 4 U CP3 ] U CP s 11212 RNA : G IBCO RNA, 100 m g, U CP s RNA 50 g, A 260g A 280 210 28S 18S, RNA U CP s U CP s 9, 200 g ), (n= 4), 11213 : U CP1 U CP2 Β2, actin U CP3 [ 1 3 ] U CP s cdna, Cybercyn T ab le 1 U CP s, 11214 R T 2PCR: RNA 10 g
396 16, U CP1 Β2actin o ligo (dt ), U CP2 U CP3 3 (an ti2sen se), g RNA M 2M L v 80 U, 75 l, 37 2 h, 5140 mu gl ), 48 h cdna ; PCR 410 l, 64% (P < 0105) 83% (P < 300 nm o lgl, T aq DNA 0101), 215 U, 50 l, PCR 92 (T ab le 2) 45 s, 55 1 m in, 72 1 m in, 35 Table 2 P lasm a gluco se and insulin levels in four group s of Table 1 DNA sequences of U CP1,U CP2,U CP3 and Β2actin gene p rim ers N am e P rim ers L ength of PCR p roducts U CP1 5 2GT GA GT TCGA CAA CT TCC GAA GT G23 Sense 620 bp 5 2CA T GA GGTCA TA T GTCA C CA GCTC23 U CP2 5 2AA CA GT TCTA CA CCAA GG GC23 Sense 479 bp 5 2A GCA T GGTAA GGGCA CA G T G23 U CP3 5 2GGA CTA CCA CCT GCTCA C T G23 Sense 302 bp 5 2CCCGTAA CA TCTGGA CTT T 23 Β2actin 5 2T T GTAA CCAA CT GGGA CG A TA T GG23 Sense 650 bp 5 2GA TCTTGA TCTTCA TGGT GCTA GG23 (P < 0101) (T ab le 2) 212 (2413 rats Gluco se (mmo lgl ) Insulin (mu gl ) Standard fed (contro l) (n= 3) overnigh t fasting (n= 3) overnigh t fasting + insulin (n= 4) 48 h fasting (n= 4) 8102 0101 3160 0148 3 3 2110 0133 3 3 6179 0125 3 24130 5140 8161 2140 3 3 13136 5123 4119 0101 3 3 Compared w ith contro l. 3 P < 0105 3 3 P < 0101 213 PCR U CP1, 2, 3 cdna, U CP1 H ind g 505, 115 bp U CP2 Sm a g 335, 145 bp U CP3 H p a g 219, 83 bp R T 2PCR 2%, (F ig. 1), 11215 PCR : PCR, 2% DNA 11216 m RNA : Im agem aster VD S 310 ( ) R T 2PCR Β2actin R T 2PCR A 100%, A g A Β2actin m RNA 11217 : ; 11218 : x θ s, t 2 F ig. 1 Restriction endonuclease m ap of U CP s PCR p rod2 211 214 UCP1 m RNA 48 h U CP1 ( 8102 0101 (F ig. 2A ) mm o lgl ) 55% 73% 15%, U CP1 m RNA ucts 1, 21U CP2 PCR p roduct digested w ithout o r w ith Sm a g ; 31PCR m arker; 4, 5. U CP3 PCR p roduct digested w ithout o r w ith H p a g ; 6, 7. U CP1 PCR p roduct digested w ithout o r w ith H ind g
3 : 21 2 3 397 47% (P < 0101), 48 h (P < 0101), ; U CP1 m RNA, U CP2 84% 99% (P < m RNA (P < 0105), 0101) (F ig. 2B ) 215 UCP2 m RNA 216 UCP3 m RNA U CP2 U CP3, U CP2 m RNA, U CP3 m RNA (F ig. 2A ), U CP2 m RNA, 48 h U CP3, 48h, U CP2 m RNA, U CP3 63% 65% (P < 0101) ; m RNA (P < 0105), U CP2 m RNA ( U CP3 25% 18% (P <, ) 0105),, 48 h U CP2 m RNA (P < 0105), 48 h 23% 28% (P < 0105), ; ; U CP2 m RNA 26% (P < 0101),, U CP3 m RNA, 48 h U CP3 m RNA U CP2 m RNA 34% 28% (F ig. 2C) (F ig. 2A ), U CP3, U CP3, 17% 19% (P < 0101) (F ig. 2D ) F ig. 2 U CP sgβ2actin mrna ratio of WA T, BA T and m uscle in rats of four group s w h ich are fed standard diet (contro l), overnight fasting (O F), overnight fasting w ith insulin adm inistration (O F+ Ins) and fasting fo r 48 hours (48hF) (A )Rep resentative bands of U CP s by RT2PCR; (B)U CP1gΒ2actin mrna ratio; (C)U CP2gΒ2actin mrna ratio; (D )U CP3gΒ2actin mrna ratio 3 P < 0105, 3 3 P < 0101, compared w ith contro l; + P < 0101, compared w ith O F
398 16 3,, U CP2 A T P, U CP2 U CP3 m RNA U CP2 [ 6 U CP3 m RNA ] (U CP1, 2, 3) 31213 U CP s, 40%, U CP s U CP3 U CP 3 U CP s m RNA, U CP 2 m RNA 311 UCPs U CP2 U CP3 m RNA 2 5 [ 7, U CP s ] [ 2, 3 ] U CP s U CP 3 U CP s, U CP 3 m RNA 312 UCPs 31211 U CP s, U CP s : 1) U CP s U CP1 2 3 2) U CP1 U CP2 3 U CP1 2 3 m RNA ;, U CP s U CP s,, U CP s U CP s U CP s, U CP s U CP s U CP1 2 U CP3, U CP2 3, 313 UCPs [ 8, ] U CP2 3 ; U CP2 3, m RNA U CP s U CP1 m RNA, [ 5 ], L ep tin ( ) [ 9 ], U CP2 U CP3, U CP s U CP2 3 U CP1,, U CP s,, 31212 U CP s, U CP s
3 : 21 2 3 399 U CP s, U CP s, U CP s,, U CP s,, ; [ 10 U CP2 U CP3 ] U CP s, U CP s U CP s Chem, 1998, 273: 5 8, (References) 1 N iso li E, Tonello C, Curruba M O. N erve grow th facto r, Β2 adreno recep to r and uncoup ling p ro tein 1 exp ression in rat brow n fat during po stnatal developm enṫ N eurosci L etṫ 1998, 246: 5 8 2 Sh im abukaro M, Zhou Y T, L ee Y. Induction of uncoup ling p ro2 tein 2 mrna by troglitazone in the pancreatic islets of Zucker diabetic fatty rats. B iochem B iop hy s R es Comm un, 1997, 237: 359 361 3 V idal2puig A, So lanes G, Grujic D, F lier J S, L ow ellb B. U CP3: A n uncoup ling p ro tein homo logue exp ressed p referentially and abundantly in skeletal m uscle and brow n adipo se tissue. B iochem B iop hy s R es Comm un, 1997, 235: 79 82 4 H jeltnes N, Fernstrom M, Zierath J R, Krook A. Regulation of U CP2 and U CP3 by m uscle disuse and physical activity in te2 trap legic subjects. D iabetolog ia, 1999, 42: 826 830 5 T eruel T, V alverde A M, N avarro P, Benito M, L o renzo M. In2 hibition of P I 32kinase and ras blocks IGF21 and insulin2induced uncoup ling p ro tein 1 gene exp ression in brow n adipocytes. J Cell P hy siol, 1998, 176: 99 109 6 M illet L, V idal H, A ndreelli F, L arrouy D, R iou J P, R icquier D, L aville M, L angin D. Increased uncoup ling p ro tein22 and23 mr2 NA exp ression during fasting in obese and lean hum ans. J C lin Invest, 1997, 100: 2665 2670 7 Bo ss O, Sam ec S, Kuhne F, B ijlenga P, A ssim acopoulous2jean2 net F, Seydoux J, Giacobino J2P,M uzzin P. U ncoup ling p ro tein2 3 exp ression in rodent skeletal m uscle is modulated by food in2 take but no t by changes in environm ental temperature. J B iol 8 W eigle D S, Selfridge L E, Schw artz M W, Seeley R J, Cum 2 m ings D E, H avel P J, Kuijpe J L, Beltrandelrio H. E levated free fatty acids induce uncoup ling p ro tein 3 exp ression in m uscle. A po tential exp lanation fo r the effect of fasting. D iabetes, 1998, 47: 298 302 9,,,. (Zhang T ie2 M ei, L in Ya2Hong, J in Jun2H ua, Yao W en2h ua. Effects of in2 sulin and food intake on OB gene exp ression in rats. Ch in J D ia2 betes), 1998, 6: 195 197 10 Sam ec S, Seydouz J, D ulloo A G. Intero rgan signaling betw een adipo se tissue m etabo lism and skeletal m usule uncoup ling p ro2 tein homo logues. Is there a ro le fo r circulating free fatty acid? D iabetes, 1998, 47: 1693 1698