2007,21 (3) :317 321 Journal of Nuclear Agricultural Sciences 317 :100028551 (2007) 032317205 (, 030801) :, ( + ) (0 013 016 019mgΠkg), LH FSH P 4 E 2,013 016mgΠkg 019mgΠkg,019mgΠkg ( P < 0105) 016mgΠkg,, 0167mgΠkg : ; ; ; EFFECTS OF SELENIUM PLEX ON SERUM REPRODUCTIVE HORMONE LEVEL DURING ESTROUS CYCLE IN SIMMENTAL HEIFERS LIU Qiang HUANG Ying2xiang YUE Wen2bin WANG Cong WANG Hao ( College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801) Abstract :Sixteen Simmental heifers were chosen and allocated into four treatments and supplemented selenium with levels of 0, 013, 016 and 019mgΠkg by selenium plex to evaluate the effects of selenium plex on reproductive hormone excretion during the oestrous cycle. The results showed that serum estradiol, luteinizing2hormone, follicle2stimulating hormone and progesterone concentrations were increased significantly ( P < 0105) by supplemental selenium plex at 013 and 016mgΠkg DM treatments compared with 019mgΠkg and control group ; and 019mgΠkg group was significantly higher than control group ( P < 0105). It was indicated that selenium plex had positive effects on reproductive hormone during estrus cycle by supplemental selenium at 016mgΠkg. Considering the selenium content in basal diet, the optimum selenium level of diet is 0167mgΠkg DM. Key words :Selenium plex ; estrous cycle ; reproductive hormone ; simmental heifer GSH2px,, [1,2 ], [3 5 ] Henry,, GSH2px 100 %, 290 % [6 ],, (LH FSH P 4 E 2 ),, 1 111, 16 18 380 kg 4, ( + ) ; :2006207219 : (02EFN211401036) ; (042S2640) : (19712),,,,,,E2mail : liuqiangabc @1631com
318 21, 013 016 019mgΠkg, 1gΠkg, Alltech,, 30d, 21d 112 ( %) : 6010 2018 410 616 418 210 016 014 (V A 150000IUΠkg V D3 60000IUΠkg V E 750IUΠ kg Fe 1500mgΠkg Cu 400mgΠkg Zn 1500mgΠkg Mn 2000mgΠkg I 1215mgΠkg Co 5mgΠkg) 018 : 6154MJΠkg 10174 % 56151 % 35159 % 0175 % 0152 % 0107mgΠkg 3 (07 :00 15 :00 23 : 00),,,, 113,,, 21h, 3h 1, 1, 1d 09 :00,, 20 ml, 30min, 2500rΠmin 10min,, - 40 114 GC2911 2 (luteinizing2hormone, LH) (follicle2 stimulating hormone,fsh) (estradiol, E 2 ) (progesterone,p 4 ), 115 SPSS 1010 One2 way2anova LSD, SPSS 1010 Regression Curve Estimation 2 211 1 1, 013 016mgΠkg LH 019mgΠkg,019mgΠkg ( P < 0105) 12h LH, 013 016mgΠkg ( P < 0105) 10d 2, 16d 3, 013 016mgΠkg 019mgΠkg,019mgΠkg ( P < 0105) 17d, LH : y = - 171861 x2 + 221426 x + 313252 ( R 2 = 019961) 1 Table 1 Effects of different supplemental selenium plex levels on serum LH at estrous period of Simmental heifers (miuπml) item 0 3131 0129 b 3 5199 0132 a 7110 0142 a 6166 0139 a 3 3186 0136 c 8168 0129 a 9126 0153 a 8120 0153 a 6 4118 0137 c 8162 0141 a 9138 0146 a 7168 0127 ab 9 5168 0135 c 10177 0152 a 12125 0138 a 9111 0129 b 12 23100 0152 b 27114 0136 a 28100 0159 a 24191 0164 ab 15 4144 0130 c 8165 0127 a 9106 0137 a 7190 0131 b 18 3149 0128 c 8161 0135 a 9173 0133 a 7185 0127 b 21 2164 0121 c 8186 0134 a 9167 0131 a 7126 0141 b 10 5107 0136 c 10118 0164 a 10157 0167 a 8176 0149 b 16 6183 0143 c 10184 0169 a 11161 0175 a 9130 0162 b 21 ( average) 4166 0151 c 9116 0169 a 9196 0172 a 8116 0153 b : ( P < 0105), Note : Data in the same row with different superscripts letters are significantly different ( P < 0105),the same as in the following tables.
3 319 019mgΠkg ( P < 0105) 16 FSH : y = - 911111 x 2 + 101756 x + 117408 ( R 2 = 019213) 212 1 Fig. 1 Effects of selenium plex on LH excretionduring the oestrous cycle of Simmental heifer 2 2, 013 016mgΠkg FSH 019mgΠkg,019mgΠkg ( P < 0105) 1 7, 8,013 016mgΠkg 019mgΠkg ( P < 0105) 9 10, 12 15,013 016mgΠkg 2 Fig. 2 Effects of selenium plex on FSH excretion 2 Table 2 Effects of supplemental selenium plex levels on serum FSH at estrous period of Simmental heifers (miuπml) 0 1142 0123 c 3 3176 0142 a 4165 0127 a 3121 0143 b 3 1149 0126 c 3114 0135 a 3198 0134 a 2153 0138 b 6 1176 0135 c 3192 0128 a 4183 0133 a 3142 0129 b 9 1154 0129 c 3174 0134 a 4168 0128 a 3126 0124 b 12 1182 0141 c 3159 0136 a 4131 0137 a 2134 0135 b 15 1168 0128 c 3124 0143 a 3195 0146 a 2151 0128 b 18 1176 0129 c 3115 0128 a 3161 0132 a 2168 0125 b 21 1151 0132 c 3136 0125 a 4112 0131 a 2172 0121 b 8 12168 0168 b 14131 0171 a 16127 0182 a 12135 0165 b 12 6149 0154 b 7170 0158 a 9136 0161 a 6142 0159 b 15 5108 0149 b 6128 0157 a 7195 0163 a 5101 0151 b 21d( average) 2152 0141 c 4103 0153 a 5112 0161 a 3141 0147 b 213 3 3, 013 016mgΠkg P 4 ( P < 0105) 1 7d,8 15d,013 016mgΠ kg ( P < 0105), P 4 : y = - 111389 x 2 + 113478 x + 015327( R 2 = 01974) 214 4 4, 013 016mgΠkg E 2 019mgΠkg,019mgΠkg ( P < 0105) 12h,013 016mgΠkg 019mgΠkg,019mgΠkg ( P < 0105), 4,5 8d,013 016mgΠkg 019mgΠkg,019mgΠkg ( P < 0105) E 2 : y = - 491806 x 2 + 651028 x + 215486( R 2 = 019905)
320 21 3 Fig. 3 Effects of selenium plex on P 4 excretion 4 Fig. 4 Effects of selenium plex on E 2 excretion 3 Table 3 Effects of supplemental selenium plex levels on serum P 4 at estrous period of Simmental heifers(ngπml) 0 0129 0116 b 0149 0112 a 0156 0109 a 0138 0108 ab 3 0138 0115 b 0155 0114 a 0163 0113 a 0148 0112 ab 6 0137 0117 b 0156 0121 a 0168 0111 a 0152 0113 ab 9 0142 0112 b 0154 0113 a 0163 0114 a 0145 0111 ab 12 0128 0111 b 0148 0112 a 0157 0122 a 0139 0112 ab 15 0140 0115 b 0179 0115 a 0188 0113 a 0165 0124 ab 18 0142 0111 b 0163 0120 a 0171 0111 a 0150 0116 ab 21 0141 0119 b 0174 0116 a 0185 0115 a 0162 0113 ab 8 0178 0121 b 1105 0122 a 1116 0121 a 0192 0123 b 9 1101 0119 b 1115 0124 a 1125 0123 a 1103 0120 b 10 1105 0118 b 1117 0117 a 1124 0121 a 1105 0123 b 11 1107 0115 b 1119 0121 a 1127 0117 a 1118 0122 b 12 1105 0123 b 1123 0119 a 1134 0122 a 1113 0127 b 13 1107 0120 b 1120 0114 a 1131 0124 a 1112 0119 b 14 1105 0118 b 1124 0120 a 1133 0120 a 1118 0128 b 15 1103 0115 b 1111 0124 a 1120 0124 a 1103 0121 c 21d( average) 0162 0125 b 0183 0131 a 0193 0136 a 0174 0129 ab FSH 3 [10 ] Akbar Bolt [11,12 ],, FSH LH FSH,, [7 ] Madej 5, 013 315ngΠml, 12h [7 ] P 4, P 4 FSH LH P 4 1 7,8 15,, 617 16ngΠml [8 ] 16, Larsson LH, LH [13 ] Smith Beckers 714h [9 ],, P 4, 6 12h P 4 [14,15 ]
Journal of Nuclear Agricultural Sciences 2007,21 (3) :317 321 321 Table 4 4 Effects of supplemental selenium plex levels on serum E 2 at estrous period of Simmental heifers(pgπml) 0 6188 1128 c 3 21108 1134 a 23101 1169 a 18115 1156 b 3 7109 1145 c 21135 1152 a 23197 1174 a 18142 1147 b 6 4198 1149 c 19107 1143 a 20104 1153 a 15199 1138 b 9 4126 1152 c 19145 1145 a 21162 1162 a 17154 1152 b 12 17164 1164 c 32117 1137 a 36109 1142 a 31119 1149 b 15 4130 1128 c 17158 1154 a 20179 1138 a 16148 1137 b 18 5106 1135 c 17173 1144 a 19161 1129 a 15139 1141 b 21 3147 1146 c 17145 1133 a 19152 1171 a 16163 1145 b 5 9184 0143 c 24199 0154 a 26185 0169 a 22172 0181 b 6 10128 0142 c 26152 0141 a 29102 0159 a 25113 0169 b 7 11107 0147 c 26195 0149 a 29146 0151 a 24188 0170 b 8 10173 0145 c 26143 0152 a 29194 0162 a 25134 0154 b 21d(,average) 6145 0178 c 20121 0181 a 22147 0198 a 18166 1102 b E 2 P 4,P 4 E 2,, [16 ], [17 ] P 4,12h, [18 ], P 4 E 2 Smith E 2, 1 1d, 2 6 8d,, 016mgΠkg DM, 0167mgΠ kgdm : [ 1 ],,,. E., 2000, (5) : 36 37 [ 2 ] Erskine R J, Eberhart R J, et al. Induction of Escherichia coli mastitis in cows fed selenium2deficient or selenium2supplemented diets. AmJ Vet Res, 1989, 50 : 2093 2100 [ 3 ] Church D C, Hansard S L, et al. Whanger. The trace elements. In : Church D C ed. Digestion Physiology and Nutrition of Ruminants. O &B Books. Inc Corvallis OR, 1971, 100 l56 [ 4 ] Paulson G D, Bauman C A, Pope A L. Metabolism of 75 Se2selenite, 75 Se2selenate, and 75 Se2selenomethionine and 35 S2sulfate by rumen microorganisms in vitro. J Anim Sci, 1968, 27 : 497 504 [ 5 ] Hudman J F, Glenn A R. Selenite uptake and incorporation by Selenomonas ruminatium. Arch Microbiol, 1984, 140 : 252 256 [ 6 ] Henry P R, Ammerman C B. Selemium bioavailability in bioavailability of nutrents for animals, Amino Acids, Minerals and Vitamins C. New York : American Acidemin Press, 1995 [ 7 ],,,.., 1996, 11 (3) : 121 127 [ 8 ] Madej A, Stupuicki R. Peripheral plasma levels of LH in the cow throught the estrus cycle. Endorkrionlogie, 1975, 65 (2) : 121 125 [ 9 ] Larsson B. Determination of ovulation by ultrasound examination and its relation to the LH2peak in heifers. Zentralbl Veterinarmed A, 1987, 34 (10) : 749 754 [10 ].. :, 1993 [11 ] Akbar A M, Reichert L E Jr, et al. Serum levels of follicle2stimulating hormone during the bovine estrus cycle. J Anim Sci, 1974, 39 (2) : 360 365 [12 ] Bolt D J, Rollins R. Development and application of radioimmunoassay for bovine follicle2stimulating hormone. J Anim Sci, 1983, 56 (1) : 146 154 [13 ],.. ( ), 2005, 23 (2) : 122 124,142 [14 ] Smith J F, Fariclough R J, Payne E. Plasma hormone levels in the cow. 11 changes in progestone and oestrogen during normal estrus cycles. New Zealand J Agri Res, 1975, 18 : 123 129 [15 ] Beckers J F, Ballman P, Ectors F. Radioimmunoa2ssay of progesterone in the cow. Sci Naturales, 1975, 28 (3) : 335 338 [16 ] Henricks D M, Dickey J F, et al. Plasma estrogen and progesterone levels after mating and during late pregnancy and postpartum in cows. Endocrinology, 1972, 90 (5) : 1336 1342 [17 ] Hafez E S E. Reproduction in Farm Animals 4th ed Edition. Publisher : Lea & Febigeer, 1980, 103 [18 ] Melampy R M, Emmerson M A, et al. The effect of progesterone on the estrous response of estrogencondition ovarieotomized cows. J Anim Sci, 1957, 16 : 967 978