Phospholipid Metabolism during the Liver Regeneration after Patial Hepatectomy Teruyoshi Yanagita, Hiroaki Yotsumoto, Kyosuke Yamamoto, Kenichi Yoshikawa
PC PC PE PC PC CHOL TG *Corresponding Authors ** 21
cell cycle HGF EGF PDGI TG Δ CHOL CHOL CoA HMG CoA PC PC PC PC CDP PE CDP CDP PC CTP CDP PC key PC PC PC Wistar mg/ml Abbott Laboratories North Chicago U. S. A methyl C methyl C New England Nuclear Boston Mass U. S. A Sintisol EX H Higgins Andersonn Wistar g g ml mg/ml No cm sham operation No g M mm Tris HCl ph mm ml Potter Elvejhem Folch g ml ml v/v ml 22
No ml KCl ml Bartlett μl ml ml ml ml nm Fletcher μl ml v/v g rpm ml ml g/dl mm ml ml nm Sperry Webb μl ml v/v g ml ml v/v rpm ml ml v/v nm Skipski μg H v/v v/v/v/v Rouser ml ml ml ml rpm nm ml ml v/v DEGS Smith Weinhold mm methyl C MTris HCl ph mm ATP Na mm MgCl ml ml mg protein rpm ml Dowex H mesh cm ml N HCl ml ml Aquasol Sintisol EX H ALOKA LSC methyl C methyl C Kent mm Tris HCl mm Mg CH COO mm CTP mm methyl 23
C μl μl mg μl TCA rpm ml charcoal mg/ml rpm charcoal ml mg/ml charcoal ml CDP rpm ml ml ml Aquasol Sintisol EX H methyl C methyl C CDP TG CHOL g CHOL TG Partial hepatectomy was performed on male wistar rats. Quiescent (0 h) and regenerating liver (from 6 h to 14 days ) were harvested and the phospholipid, triglyceride and cholesterol concentration were measured as described in Materials and Methods. In the figures, 1, 2, 3 and 4 days mean 24 h, 48 h, 72 h and 96 h respectively after partial hepatectomy. PC PE Sph LysoPC PA PS PI Partial hepatectomy was performed on male wistar rats. Quiescent (0 h) and regenerating liver (from 6 h to 14 days) were harvested and the phospholipid composition in liver total lipids were measured as described in Materials and Methods. In the figures, 1, 2, 3 and 4 days mean 24 h, 48 h, 72 h and 96 h respectively after partial hepatectomy. 24
Partial hepatectomy was performed on male wistar rats. Quiescent (0 h) and regenerating liver (from 6 h to 14 days) were harvested and the fatty acid composition in liver total lipids were measured as described in Materials and Methods. In the figures, 1, 2, 3 and 4 days mean 24 h, 48 h, 72 h and 96 h respectively after partial hepatectomy. Partial hepatectomy was performed on male wistar rats. Quiescent (0 h) and regenerating liver (from 6 h to 14 days) were harvested and the activity of choline kinase and cytidylyltransferase were measured as described in Materials and Methods. In the figures, 1, 2, 3 and 4 days mean 24 h, 48 h, 72 h and 96 h respectively after partial hepatectomy. Trigger PC key HMG CoA Fex P CDP PC Fex TG TG TG CHOL Fex CHOL CHOL HMG CoA CHOL HMG CoA 25
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Phospholipid Metabolism during the Liver Regeneration after Patial Hepatectomy Teruyoshi Yanagita, Hiroaki Yotsumoto, Kyosuke Yamamoto, Kenichi Yoshikawa Department of Health and Nutrition Sciences, Faculty of Health and Nutrition Sciences, Nishikyushu University Department of Applied Biochemistry and Food Science, Saga University Department of Internal Medicine, Saga University (Takagi Hospital) Accepted: February Abstract The present study was conducted to understand the alteration of lipid metabolism during liver regeneration after 70% partial hepatectomy. The alteration of liver phospholipid, triglyceride and cholesterol concentrations and enzyme activities responsible for phospholipid biosynthesis were measured after partial hepatectomy in rats until 14 days. The concentration of liver phospholipid tended to increase after 24 hr of partial hepatectomy. The ratio of phosphatidylcholine decreased whereas the ratio of phosphatidylethanolamine increased in the liver after partial hepatectomy. The activity of cytidylyltransferase was significantly enhanced from 12 hr after partial hepatectomy. The increase of cytidylyltransferase activity continued for 72 hr. On the other hand, the activity of choline kinase increased only 3 hr after partial hepatectomy. The ratio of linoleic acid in the liver increased about 2 times and arachidonic acid decreased from 6 hr to 72 hr, compared to the controls. During liver regeneration, cholesterol increased whereas triglyceride decreased after 24 hr of partial hepatectomy. The present study suggests that cytidylyltransferase is important for phospholipid biosynthesis during liver regeneration. Key words liver regeneration, phospholipid, triglyceride, cytidylyltransferase, hepatectomy 27