Chinese Journal of Zoology 2009,44 (3) :1 8 3 ( 410128 ; 410081) : 3 5 7 9 11 d, ( Hyriopsis cumingii), ( H. cumingii Plague Virus,HcPV) :, 7 d,,7 d ;,,, :, 7 d,7 d,, ;,,,,, : ; ; ; ; :Q955 :A :025023263 (2009) 03201208 Effects of Hyriopsis cumingii Plague Virus on Digestive Organs of the H1 cumingii XIAO Tiao2Yi LIU Qiao2Lin ZHANG Huai2Yun 3 GE Xi2Kai XU Bao2Hong SU Jian2Ming ZHONGLei ( College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128 ; Central South University of Forestry and Technology, Changsha 410081, China) Abstract :The pathological changes of digestive organs were observed by optical microscopy and electron microscopy at the 3rd, 5th, 7th, 9th, and 11th day after artificial infection of Hyriopsis cumingii with the pathological tissue liquid. The results showed that the H. cumingii Plague virus damaged the structure of the digestive organs. The liver was most seriously damaged. This digestive gland was swelling and its lumen was smaller at the 7th day of infection,and after that vacuolation and coenocytes were observed. Under electron microscope,the organelles such as mitochondria and endoplasmic reticulum were damaged and the virus particles were frequently observed. Pathological changes also included stomach and intestine damages. Their structure damage was not obvious within 7 days of infection,but after the 7th day the columnar cells became swellen and the cilia were detached from the epithelial cells. Under electron microscope, organelles damage and even vacuolation were observed. The virus particles became more frequently observed at later stage of infection,but less than observed in the liver. Key words : Hyriopsis cumingii ; H. cumingii Plague Virus ; Digest gland ; Enteron ; Histopathology (No. 06NK3049), (No. 07A012) ; 3,E2mail :hyzhang @public. cs. hn. cn ;,, ; : ; E2mail :tyx1128 @yahoo. com. cn :2008210231, :2009203216
2 Chinese Journal of Zoology 44 ( Hyriopsis cumingii) (Mollusca) (Lamellibranchia) ( Eulamellibranchia) (Unionidae), [1 ],, [2 ] 20 80, Bouin,, [3 8 ] 2005 9,, [9 ], 1 111 : ( ), 50 %, - 20 :, 618 1913 cm, 412 1018 cm, 117 512 cm,,,, 112 11211, 3, ( ), ph 712 PBS 1 10,4 3 000 rπmin 30 min,, ( ) 5 000 IUΠ ml,4 16 h, 11212 ( 10 ) ( 15 ), 015 mlπ PBS ; 015 mlπ, 23 28 11213, 3 5 7 9 11 d 3,, 4, 50 %, 11214 Bouin, ( 4 6 m),h. E,Motic1300 11215 215 %, 1 %,, Epon 812,, 2,J EM21230 80 kv 2 211 21111 ( :1 6),,,, H. E, ; ( :1) 3 d,, ( :2) ; 5 d, ( :3) ; 7 d ( :4) ; 9 d,,, ( :5) ; 11 d,,,, ( :6) 21112 ( :7 12),,,,
3 : 3,,, ( :7), 3 d, ( :8) ; 5 d ( :9) ; 7 d, ( :10) ; 9 d, ( :11) ; 11 d, ( :12) 21113 ( :13 18),,,, ( :13) 3 d,, ( :14) ; 5 d, ( : 15) ; 7 d, ( :16) ; 9 d, ( :17) ; 11 d,,, ( :18) 212 21211 ( :19 24),,,, ( :19) ; 3 d,,, ( :20) ; 5 d ( :21),,, ( :22) ; 9 d, ( :23) ; 11 d,, ( :24) 21212 (, :25 30), ( :25) ; 3 d,, ( :26) ; 5 d, ( :27) ; 7 d,, ( :28) ; 9 d ( :29) ; 11 d ( :30) 3,,, [10,11 ], ( ) [12 16 ], [17 ] ( Argopectens irradias lamarck) ; [18 ] ; [19 ] ( Chlamys f arreri) ; [20 ] ; [21 ] ; [9 ],,, 311,, ( Ostrea talienwhanensis crosse ) [22 ] ( Solen gouldii) [23 ] ( Mytilus galloprovincialis) [24 ] [25 ] ( Meretrix meretrix) [26 ],,,,
4 Chinese Journal of Zoology 44, 3 d,,,, [9 ], [18 ], [27 ] ( ),, [18 ] [28 ], [29 ] ( Haliotis diversicolor) ( ),,,,,, [30 ],,,9 d,9, [31 ],,, 312, :,, 7 d,7 d, [27 ], :,,, ;,,,,, [32 ] ( ) ( ) ( ),, [33 ],,,,,,,,, d,,,,,,,,,,,,, ( ),,,,,
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6 Chinese Journal of Zoology 44 [30 ],.. ( ),1998,35 (1) :102 1061 [31 ] Johnson M A, Paulet Y M, Donval A, et al. Histology, histochemistry and enzyme biochemistry in the digestive system of the endosymbiont bearing bivalve Loripes lucinalis (Lamarck ). Journal of Experimental Marine Biology and Ecology,1996,197 :15 381 [32 ],,.. ( ),1995,18(4) :134 1371 [33 ],,..,1994, (5) :3471 [34 ],,..,1995,19 (2) :187 1891 1 6,7 12,13 18, 400, = 50 m 11 ; 21 3 d ; 31 5 d ; 41 7 d ; 51 9 d ; 61 11 d ; 71 ; 81 3 d ; 91 5 d ; 101 7 d ; 111 9 d ; 121 11 d ; 131 ; 141 3 d ; 151 5 d ; 161 7 d ; 171 9 d ; 181 11 d Explanation of Plate Microstructural pathological changes of digestive organs in the H. cumingii 1-6 are livers,7-12 are stomaches,and 13-18 are midguts, 400,Bar = 50 m. 11Healthy mussels liver ; 21The liver 3 days after infection ; 31The liver 5 days after infection ; 41The liver 7 days after infection ; 51The liver 9 days after infection ; 61The liver 11 days after infection ; 71Healthy mussels stomach ; 81The stomach 3 days after infection ; 91The stomach 5 days after infection ; 101The stomach 7 days after infection ; 111The stomach 9 days after infection ; 121The stomach 11 days after infection ; 131Healthy mussels midgut ; 141The midgut 3 days after infection ; 151The midgut 5 days after infection ; 161The midgut 7 days after infection ; 171The midgut 9 days after infection ; 181The midgut 11 days after infection. GE. ; L. ; G. ; Va. ; EC. ; S. ; ML. ; MF. ; MC. ; CC. ; Pk. GE. Gland epithelium ; L.Lumen ; G. Gland ; Va. Vacuole ; EC. Epithelial cells ; S. Submucosa ; ML. Mucosal layer ; MF. Muscle fibers ; MC. Mucus cells ; CC. Columnar cells ; Pk. Polykaryon. 19 24,25 30, = 500 nm 191, 30 000 ; 201 3 d, 30 000 ; 211 5 d, 100 000 ; 221 7 d, 100 000 ; 231 9 d, 100 000 ; 241 11 d, 50 000 ; 251, 8 000 ; 261 3 d, 100 000 ; 271 5 d, 100 000 ; 281 7 d, 20 000 ; 291 9 d, 40 000 ; 301 11 d, 50 000 Explanation of Plate Ultrastructural pathological changes of digestive organs in the H. cumingii 19-24 are livers, 25-30 are visceral missions, Bar = 500 nm. 191Health mussels liver, 30 000 ; 201The liver 3 days after infection, 30 000 ; 211The liver 5 days after infection, 100 000 ; 221The liver 7 days after infection, 100 000 ; 231The liver 9 days after infection, 100 000 ; 241The liver 11 days after infection, 50 000 ; 251Health mussels visceral mission, 8 000 ; 261The visceral mission 3 days after infection, 100 000 ; 271The visceral mission 5 days after infection, 100 000 ; 281The visceral mission 7 days after infection, 20 000 ; 291The visceral mission 9 days after infection, 40 000 ; 301The visceral mission 11 days after infection, 50 0001 ER. ; Cb. ; VP. ; Mi. ; Va. ; CT. ER. Endoplasmic reticulum ; Cb. Cytoblast ; VP. Virus particle ; Mi. Mitochondrion ; Va. Vacuole ; CT. Connective tissue.
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