19 14 Vol. 19 No. 14 2009 7 China Journal of Modern Medicine Jul. 2009 1005-8982 2009 14-2094- 04 1,2 2 1 2 2 2 [1. 41 0008 2. 51 1 470] 2006 12 ~2009 1 204 108 204 163 79.9% 108 46 42.6% P <0.0001 89.6% 57.9% 70.0% P <0.0001 204 159 69.2% 5.7% 25.2% 45 11.1% 22.2% 66.7% P <0.0001 R 657.42 A Clonorchis sinensis eggs involved in the formation of gallbladder stones QIAO Tie 1,2, MA Ruihong 2, ZHANG Yangde 1, FENG Yuyang 2, WANG Xingqiang 2, WANG Xiaofeng 2 (1.National Hepatobiliary and Enteric Surgery Research Center, Central South University, Changsha, Hunan, 410008 P.R.China; 2. the Second People s Hospital Panyu, Institute of Gallbladder Disease of NHEC, Institute of Gallbladder Disease, Guangzhou, Guangdong 511470, P.R. China) Abstract: Objective To investigate the relationship between clonorchiasis infection and cholecystolithiasis. Methods In our present study, we chose 204 portions of gallbladder stones by random from 204 patients who accepted gallbladder-retention cholelithotomy during 2006.12~2009.01 at general surgery department in our hospital, and then we grinded them to explored the internal structure and construction. Meanwhile, 108 portions of homochronous bile were chose randomly, and bile sediment was obtained through centrifugalization, and then slides were smeared and tested by microscopy. Results 163 of the 204 cases of gallbladder stones were detected C. sinensis eggs in the core (79.9%), while 46 of the 108 cases of bile could be detected C. sinensis eggs (42.6%), and they had obvious statistical difference (P <0.0001). The detection rate of C. sinensis eggs was 89.6%, 57.9% and 70.0% in the pigment calculus, cholesterol and mixed calculus respectively, which had obvious statistical difference (P <0.0001). 159 of the 204 cases were from clonorchiasis-high-incidence area, such as Guangdong and Guangxi (Pigment calculus, cholesterol and mixed calculus occupied 69.2%, 5.7% and 25.2% respectively), 45 of them were from clonorchiasis-low-incidence area, such as Gansu and Sichuan (Pigment calculus, cholesterol and mixed calculus occupied 11.1%, 22.2% and 66.7% respectively.), the constituent ratio of the two groups had obvious statistical difference (P <0.0001). Conclusions Our data for the first time demonstrated that clonorchiasis infection had a close relationship with Cholecystolithiasis: we found that it was mainly pigment calculus in clonorchiasis-high-incidence area 2009-06- 11 2094
14 and cholesterol and mixed calculus in clonorchiasis-low-incidence area and the detection rate of C. sinensis eggs in pigment calculus was much higher than that in cholesterol and mixed calculus; microscopic examination of gallbladder stones maybe the economical, easy and effective method for the diagnosis of clonorchiasis infection. Keywords: Pigment Calculus; Cholesterol Calculus; Mixed Calculus; Clonorchis sinensis eggs; Cholecystolithiasis SPSS11.5 χ 2 LSD x±s P <0.05 [1] 2006 12 ~2009 1 204 2 108 2.1 1 1.1 2006 12 ~2009 1 204 123 81 45.3±12.4 204 108 >70% 1a A >70% 1b B <6 1c C 204 mm 19 9.31% 115 56.37% 70 34.31% CHiAO ZL200820188856.6 >5 mm <5 mm CHiAO ZL200720049447.3 1.2 BX51 100 400 10 10 40 1~3 0.3~1.5 cm 260 2 3 100 400 1.3 BX51 3 000 r/min 10 min 100 400 100 400 a A b B c C 1 2 1 2 100 1 400 2.2 1.4 2095
19 204 163 29μm 17μm 79.9% 19 3 11 57.9% 2a A 70 49 2 3 70.0% 1b B 115 2.3 103 89.6% 2c 108 46 C 10% 42.6% 46 2 90% 100% 62 36 58.1% 4 43.5% 90% 10% 100 400 100 400 a A b B c C 3 4 95 62 1997~2003 862 393 18% [2] 78.5%~85.0% [3] 10 100 2096
14 1 000 [4] [8] [5] [6] 204 163 79.9% 108 46 42.6% 3 P <0.0001 β- 204 19 9 10 115 110 5 70 40 30 [9] 5.7% 69.2% 25.2% 90% 22.2% 11.1% 66.7% 90% 1 P <0.0001 1 P 110 5 115 <0.0001 9 10 19 [7] 40 30 70 159 45 204 57.9% 70.0% 89.6% 2 2 P <0.0001 % P 103 12 115 89.6 <0.0001 11 8 19 57.9 49 21 70 70.0 163 41 204 79.9 2101 2097
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