JCVI Locus Protein Name. JCVI Role Category JCVI Locus (LT2) Annotation name

Additional File 4. Detailed information on all 41 genes that showed evidence for positive selection from positive selection analysis, in which gene Alignment Primary Gene Genbank Locus JCVI Locus Protein Name JCVI Role Category JCVI Locus (LT2) Annotation name (LT2) (CT18) Length, No. (LT2) bp pyridoxal kinase phosphoglucomutase, alpha-dglucose phosphate-specific PTS system IIA component, putative oligopeptide ABC transporter, ATPbinding protein conserved hypothetical protein TIGR00156 domain protein phosphoribosylaminoimidazole carboxylase, catalytic subunit Biosynthesis of cofactors, prosthetic groups, and carriers NT01ST1789 AAL20372 STM1450 NT03ST1716 666 pgm Energy metabolism NT01ST0841 AAL19642 STM0698 NT03ST0765 1638 oppf pure Transport and binding proteins Transport and binding proteins NT01ST4092 AAL22130 STM3258 NT03ST3550 462 NT01ST4531 AAL22487 STM3626 NT03ST4332 1011 Hypothetical proteins NT01ST1873 AAL20434 STM1515 NT03ST1581 384 Purines, pyrimidines, nucleosides, and nucleotides NT01ST0653 AAL19488 STM0534 NT03ST0601 507 rard protein rard Unknown function NT01ST4920 AAL22799 STM3955 NT03ST3727 879 malt regulatory protein. malt Energy metabolism NT01ST4393 AAL22377 STM3515 NT03ST4451 2703 glycyl-trna synthetase, beta subunit glys Protein synthesis NT01ST4569 AAL22514 STM3655 NT03ST4306 2067 nrdi protein nrdi Purines, pyrimidines, nucleosides, and NT01ST3491 AAL21691 STM2806 NT03ST3017 408 nucleotides hypothetical protein Hypothetical proteins NT01ST4989 AAL22854 STM4015 NT03ST4006 846 acetate operon repressor. iclr Energy metabolism NT01ST5191 AAL23011 STM4187 NT03ST4591 819 conserved hypothetical protein Hypothetical proteins NT01ST5283 AAL23082 STM4258 NT03ST4649 1386 exonuclease SbcC, putative DNA metabolism NT01ST0490 AAL19349 STM0395 NT03ST0438 3096 hypothetical protein Hypothetical proteins NT01ST1895 AAL20451 STM1532 NT03ST1566 678 aminotransferase, class I arat Central intermediary metabolism NT01ST0731 AAL19554 STM0603 NT03ST0667 1158 salmonella atypical fimbria outer membrane usher safc Cell Envelope NT01ST0369 AAL19258 STM0301 NT03ST0340 2508 lipoprotein, putative Cell Envelope NT01ST0899 AAL19687 STM0743 NT03ST0813 273 PipB pipb Unclassified NT01ST1346 AAL20020 STM1088 NT03ST1134 873

membrane protein, putative Cell Envelope NT01ST1778 AAL20363 STM1441 NT03ST1726 1995 oligopeptide ABC transporter, Transport and binding periplasmic oligopeptide-binding oppa NT01ST2082 AAL20596 STM1679 NT03ST1411 1605 proteins protein GDP-mannose mannosyl hydrolase Purines, pyrimidines, nucleosides, and nucleotides NT01ST2623 AAL21011 STM2107 NT03ST2358 435 proton/peptide symporter family protein Unclassified NT01ST4488 AAL22452 STM3592 NT03ST4374 1470 aldehyde dehydrogenase B aldb Energy metabolism NT01ST4599 AAL22539 STM3680 NT03ST4277 1536 3-hydroxyisobutyrate dehydrogenase, putative Energy metabolism NT01ST4997 AAL22862 STM4023 NT03ST3998 840 histidinol phosphatase-related protein Unknown function NT01ST0303 AAL19211 STM0248 NT03ST0283 573 glycosyl hydrolase, family 13 malz Energy metabolism NT01ST0499 AAL19355 STM0401 NT03ST0447 1815 conserved hypothetical protein Hypothetical proteins NT01ST1594 AAL20205 STM1280 NT03ST1882 396 multidrug resistance protein norm Cellular processes NT01ST1758 AAL20347 STM1425 NT03ST1745 1371 outer membrane protein c precursor ompc Cell Envelope NT01ST2829 AAL21169 STM2267 NT03ST2542 1134 CorE b2611 Viral functions NT01ST3328 AAL21567 STM2678 NT03ST2946 750 similar to Cell Envelope NT01ST3484 AAL21686 STM2801 NT03ST3011 300 ubih protein ubih Biosynthesis of cofactors, prosthetic groups, and NT01ST3824 AAL21932 STM3057 NT03ST3313 1176 carriers conserved hypothetical protein TIGR01212 Hypothetical proteins NT01ST4169 AAL22198 STM3329 NT03ST3620 927 conserved hypothetical protein Hypothetical proteins NT01ST4329 AAL22325 STM3463 NT03ST4512 201 acetyltransferase, GNAT family family Unknown function NT01ST4455 AAL22425 STM3565 NT03ST4412 381 PTS system, mannitol-specific IIC Transport and binding component subfamily, putative proteins NT01ST4604 AAL22544 STM3685 NT03ST4272 1914 catalase/peroxidase HPI katg Cellular processes NT01ST5107 AAL22946 STM4106 NT03ST3894 2178 99 pct identical to TraF of plasmid R64 Viral functions NT01ST5263 AAL23066 STM4242 NT03ST4633 1284 conserved hypothetical protein Hypothetical proteins NT01ST1047 AAL19797 STM0861 NT03ST0922 471 hypothetical protein Hypothetical proteins NT01ST2302 AAL20769 STM1854 NT03ST2029 162

a d N = number of non-synonymous changes per non-synonymous sites b d S = number of synonymous changes per synonymous sites c TO = overall test; Ch#= Choleraesuis branch specific test; Pty#= Paratyphi A branch specific test ; Ty# = Typhi branch specific test; Tym#= Typhimurium br d Proportion of sites under positive selection e w =omega (i.e., d N /d S ) f only sites with a probability >95% as being under positive seletion are shown; the first number indicates the aa sites identified by Bayes Empirical Bayes (B bracket indicates the posterior probability; ";" separates the aa sites; aa sites are based on site location in the alignment used for positive selection analyses File 5); BEB method is used to calculate posterior probabilities for site classes under alternative models (Model 2a and Model A if the LRT suggests presenc positive selection on the foreground branch) g Each distinct gene tree (n=18) was designated with a unique gene tree number h Gene trees used for positive seletion analyses are shown in Newick format; ATCC9150 = S. Paratyphi A, ch= S. Choleraesuis, CT18= S. Typhi CT18, Ty2= i Nc= effective number of codons used in a gene sequence was calculated using the program chips from EMBOSS package; Nc can take values from 20 (in where one codon is exclusively used for each amino acid) to 61 (which indicates that the use of alternative synonymous codons is equally likely (Wright. 199

es without recombination were used d N a d S b Estimated diversity No. of informative sites p-value for TO c q-value for TO Prop. of sites under pos sel. (TO) d ω (TO) e BEB (TO) f p-value for Ch# c q-value for Ch# 0.0109 0.0415 0.0100 7 0.0979 1.0000 0.0047 85.5050 1.0000 1 0.0069 0.0879 0.0140 24 0.5233 1.0000 0.0022 17.4805 1.0000 1 0.0622 0.1733 0.0510 39 0.0044 0.9246 0.1068 14.5336 139 (0.984) 1.0000 1 0.0069 0.1045 0.0160 19 0.0462 1.0000 0.0035 27.5550 1.0000 1 0.0436 0.1282 0.0330 17 0.0291 1.0000 0.0471 10.9797 108 (0.976) 1.0000 1 0.0169 0.1800 0.0320 25 0.0371 1.0000 0.0198 13.6957 1.0000 1 0.0070 0.1075 0.0130 14 0.0050 0.9246 0.0041 62.9697 293 (0.969) 0.9989 1 0.0053 0.0652 0.0100 26 0.4663 1.0000 0.0229 2.7717 0.94770 1 0.0205 0.4399 0.0640 204 0.8642 1.0000 0.0599 1.2638 1.0000 1 0.0152 0.0985 0.0150 5 0.0984 1.0000 0.0076 246.4417 1.0000 1 0.0196 0.0520 0.0160 19 0.0557 1.0000 0.0057 40.1930 1.0000 1 0.0053 0.0984 0.0140 7 0.4262 1.0000 0.0099 5.8102 1.0000 1 0.0160 0.0679 0.0130 20 0.1991 1.0000 0.0617 4.7258 1.00000 1 0.0071 0.0648 0.0110 34 0.9752 1.0000 0.0060 5.5276 1.0000 1 0.0142 0.0657 0.0170 15 0.2732 1.0000 0.0373 8.2401 1.0000 1 0.0025 0.0768 0.0120 16 0.1535 1.0000 0.0029 13.3770 1.0000 1 0.0254 0.0440 0.0120 29 1.0185E-05 0.0103 0.0688 9.1453 85 (0.951); 111 (0.955); 405 (0.954); 692 (0.950) 1.0000 1 0.0277 0.0521 0.0150 3 0.0437 1.0000 0.0545 14.3698 0.0005 0.183048 0.0241 0.0402 0.0130 13 0.0001 0.0688 0.0115 81.6436 173 (0.997) 0.0182 1

0.0066 0.0456 0.0080 14 0.0120 1.0000 0.0015 999.0000 2.85E-06 0.004334 0.0108 0.0508 0.0090 9 0.1146 1.0000 0.0019 136.1688 1.19E-05 0.012115 0.0424 0.1124 0.0240 10 0.1428 1.0000 0.0153 60.8052 1.0000 1 0.0061 0.0508 0.0090 10 1.3577E-05 0.0103 0.0021 999.0000 1.0000 1 0.0073 0.1526 0.0200 36 0.0879 1.0000 0.0020 163.0558 1.0000 1 0.0141 0.0318 0.0080 5 0.0572 1.0000 0.0424 17.2693 1.82E-05 0.013876 0.0456 0.1285 0.0250 4 0.0013 0.4820 0.0942 8.2212 175 (0.985); 184 (0.959) 3.36E-08 0.000102 0.0173 0.1270 0.0200 35 1.0000 1.0000 0.0782 1.0000 1.0000 1 0.0114 0.0578 0.0130 5 0.0718 1.0000 0.0174 21.6655 1.0000 1 0.0042 0.0374 0.0060 2 0.0046 0.9246 0.0026 999.0000 1.0000 1 0.0078 0.0683 0.0090 3 0.0354 1.0000 0.0057 52.9941 0.0002 0.098647 0.0058 0.0246 0.0050 4 0.0153 1.0000 0.0093 29.7078 0.0003 0.141156 0.0095 0.0902 1.2000 0 0.0088 1.0000 0.0102 41.1050 100 (0.955) 0.99887 1 0.0049 0.0798 0.0120 16 0.0237 1.0000 0.0026 182.7346 171 (0.983) 1.0000 1 0.0059 0.0431 0.0070 3 0.0196 1.0000 0.0037 71.4830 0.0003 0.147094 0.0136 0.0396 0.8000 0 0.0055 0.9332 0.0154 999.0000 1.0000 1 0.0201 0.1310 0.0200 2 0.0755 1.0000 0.0132 22.0877 121 (0.964) 1.0000 1 0.0022 0.1546 0.0190 41 2.2885E-06 0.0035 0.0016 999.0000 1.0000 1 0.0050 0.0657 0.0090 16 1.2396E-06 0.0035 0.0014 999.0000 1.0000 1 0.0132 0.0224 0.0070 8 0.0743 1.0000 0.0023 508.4250 1.0000 1 0.0102 0.0277 0.6000 0 0.0011 0.4716 0.0144 600.3252 1.0000 1 0.0886 0.1922 4.0000 1 0.0062 0.9526 0.1384 114.4740 45 (0.983) 1.00000 1

ranch specific test BEB), the number in s (provided as Additional ce of codons under = Typhi Ty2, LT2= Typhimurium LT2 case of extreme bias 90. Gene 87:23-29)

Prop. of sites under pos sel. ω(ch#) e p-value BEB (Ch#) f (Ch#) d for Tym# c q-value for Tym# Prop. of sites under pos sel. (Tym#) d ω(tym#) e BEB (Tym#) f p-value Ty# c q-value Ty# 0.0000 1.0000 1.0000 1 0.2121 1.0743 0.0000 0.014705 0.0822 1.0000 1.0000 1 0.0000 1.0000 0.0001 0.015691 0.0015 1.0000 1.0000 1 0.0000 1.0000 0.0001 0.015691 0.0000 1.0000 1.0000 1 0.0460 1.0000 0.0001 0.015691 0.1978 1.0000 1.0000 1 0.0000 1.0000 0.0001 0.01672 0.0000 1.0000 1.0000 1 0.0000 1.0000 0.0001 0.019379 0.0000 1.0000 1.0000 1 0.0000 1.0000 0.0001 0.019379 0.0200 1.0000 1.0000 1 0.0000 1.0000 0.0001 0.01979 0.1092 1.0000 1.0000 1 0.0000 1.0000 0.0003 0.039258 0.0000 1.0000 1.0000 1 0.0000 1.0000 0.0006 0.069333 0.0000 1.0000 0.4151 1 0.7238 228.8519 0.0007 0.069333 0.0000 1.0000 1.0000 1 0.0000 1.0000 0.0008 0.069333 0.0000 1.0000 1.0000 1 0.2313 1.0000 0.0010 0.088403 0.0089 1.0000 1.0000 1 0.0036 1.0000 0.0013 0.104098 0.1694 1.0493 1.0000 1 0.0923 1.0547 0.0022 0.161412 0.0000 1.0000 1.0000 1 0.0000 1.0000 0.0029 0.197573 0.0000 1.0000 1.0000 1 0.0000 1.0000 0.0114 0.503702 0.0242 999.0000 1.0000 1 0.0000 1.0000 0.2776 1 0.0213 999.0000 173 (0.991); 178 (0.973); 261 (0.973) 1.0000 1 0.0000 1.0000 1.0000 1

0.0018 999.0000 1.0000 1 0.0000 1.0000 1.0000 1 0.0051 933.5563 1.0000 1 0.0000 1.0000 1.0000 1 0.0000 1.0000 0.0003 0.127727 0.0056 999.0000 1.0000 1 0.0000 1.0000 1.0000 1 0.0000 1.0000 0.3894 1 0.0429 1.0000 1.0000 1 0.0214 1.0000 0.9989 1 0.0048 999.0000 1.0000 1 0.0051 1.0000 1.0000 1 0.0653 999.0000 175 (0.999);184 (0.997); 185 (0.992); 191 (0.982) 1.0000 1 0.0000 1.0000 ND ND 0.0982 1.0000 0.0001 0.100555 0.0055 766.8320 ND ND 0.0000 1.0000 0.0000 0.014475 0.0169 999.0000 ND ND 0.0000 1.0000 0.0000 0.014475 0.0023 999.0000 ND ND 0.0065 999.0000 274 (0.970) 1.0000 1 0.0000 1.0000 ND ND 0.0088 115.3601 1.0000 1 0.0000 1.0000 ND ND 0.0000 1.0000 0.9989 1 0.0000 1.0000 ND ND 0.0000 1.0000 0.0002 0.111486 0.0029 999.0000 ND ND 0.0037 999.0000 1.0000 1 0.0000 1.0000 ND ND 0.0033 1.0994 0.0001 0.100555 0.0154 999.0000 ND ND 0.7660 1.0000 1.0000 1 0.0000 1.0000 ND ND 0.0075 1.0000 1.0000 1 0.0000 1.0000 ND ND 0.0000 1.0000 0.8723 1 0.0458 1.7945 ND ND 0.0000 1.0000 1.0000 1 0.2490 1.0501 ND ND 0.1547 1.0499 0.0002 0.111486 0.0143 600.5735 ND ND 0.0001 1.0000 1.0000 1 0.0000 1.0000 ND ND

Prop. of sites under pos sel. (Ty#) d ω (Ty#) e BEB (Ty#) f p-value for Pty# c q-value for Pty# Prop. of sites under pos sel. (Pty#) d ω (Pty#) e BEB (Pty#) f Tree group no g 0.0068 999.0000 0.9977 1 0.0194 1.0981 7 0.0035 999.0000 1.0000 1 0.0253 1.0000 5 0.1064 50.4382 124 (0.967); 139 (0.996); 143 (0.984); 144 (0.970); 147 (0.977) 1.0000 1 0.0000 1.0000 5 0.0047 334.8240 1.0000 1 0.0000 1.0000 5 0.0449 998.9997 1.0000 1 0.0000 1.0000 5 0.0207 769.2387 1.0000 1 0.0000 1.0000 5 0.0076 999.0000 1.0000 1 0.0122 1.0705 5 0.0050 118.3799 801 (0.979) 1.0000 1 0.0000 1.0000 8 0.0126 619.1814 313 (0.966) 1.0000 1 0.1156 1.0000 508 (0.568) 7 0.0093 999.0000 1.0000 1 0.2174 1.0000 5 0.0047 365.6776 1.0000 1 0.1401 1.0512 7 0.0040 625.2594 1.0000 1 0.0077 1.0000 5 0.0078 99.4822 1.0000 1 0.1545 1.0000 8 0.0021 999.0000 1.0000 1 0.0000 1.0000 7 0.0050 999.0000 1.0000 1 0.1436 1.0000 7 0.0029 176.6151 1.0000 1 0.0000 1.0000 5 0.0015 252.8766 1.0000 1 0.0000 1.0000 5 0.8552 999.0000 10 (0.956); 31(0.955) 1.0000 1 0.0000 1.0000 5 0.0000 1.0000 1.0000 1 0.0000 1.0000 7

0.0451 1.0000 0.9977 1 0.0000 1.0000 7 0.1483 1.0000 1.0000 1 0.2629 1.0000 7 0.2336 1.0000 1.0000 1 0.1929 1.0000 5 0.9175 307.3132 0.5810 1 0.0439 11.2062 7 0.0117 1.0000 0.0000 0.020085 0.0021 999.0000 5 0.3294 1.0000 1.0000 1 0.0000 1.0000 7 ND ND ND 1.0000 1 0.0000 1.0000 1 ND ND ND 1.0000 1 0.0549 1.0000 2 ND ND ND 1.0000 1 0.1570 1.0479 1 ND ND ND 0.9952 1 0.0000 18.5210 1 ND ND ND 1.0000 1 0.0000 1.0000 1 ND ND ND 0.9970 1 0.9243 1.0833 1 ND ND ND 0.0000 0.020085 0.0104 999.0000 9 ND ND ND 0.8948 1 0.0018 13.2216 6 ND ND ND 0.4423 1 0.9482 574.0819 1 ND ND ND 1.0000 1 0.0000 1.0000 1 ND ND ND 0.0001 0.039146 0.0176 999.0000 1 ND ND ND 1.0000 1 0.0000 1.0000 1 ND ND ND 1.0000 1 0.0000 1.0000 2 ND ND ND 0.0000 0.032921 0.0030 999.0000 6 ND ND ND 1.0000 1 0.1392 1.0500 6 ND ND ND 0.0003 0.197335 0.1489 999.0000 32 (0.992); 40 (0.992); 9 44 (0.958); 45 (0.977)

Gene Tree h p value for NSS q-value for NSS p-value Max Chi q-value for Max Chi Permutationbased p-value for PHI q-value for PHI GENE- CONV inner p-value q-value for GENE-CONV Nc value for Paratyphi A i ((ATCC9150,(CT18,Ty2)),ch,LT2); 1 1 0.12 0.564158 1 1 0.0268 0.2203 50.37 ((ATCC9150,ch),(CT18,Ty2),LT2); 0.461 1 0.595 1 0.751 1 0.3602 0.7053 40.168 ((ATCC9150,ch),(CT18,Ty2),LT2); 1 1 0.531 1 1 1 0.5319 0.8173 41.777 ((ATCC9150,ch),(CT18,Ty2),LT2); 1 1 0.162 0.65189 1 1 0.0732 0.3644 42.234 ((ATCC9150,ch),(CT18,Ty2),LT2); 1 1 0.096 0.522952 1 1 0.1754 0.5236 44.925 ((ATCC9150,ch),(CT18,Ty2),LT2); 1 1 0.993 1 1 1 0.1444 0.4809 41.525 ((ATCC9150,ch),(CT18,Ty2),LT2); 1 1 0.427 0.947453 1 1 0.0955 0.4087 45.46 (ATCC9150,(ch,(CT18,Ty2)),LT2); 0.169 0.992744 0.011 0.154379 0.15 1 0.3114 0.6776 42.459 ((ATCC9150,(CT18,Ty2)),ch,LT2); 0.019 0.322208 0.008 0.122538 0.23 1 0.1544 0.4936 39.453 ((ATCC9150,ch),(CT18,Ty2),LT2); 1 1 0.761 1 1 1 0.2911 0.6693 49.358 ((ATCC9150,(CT18,Ty2)),ch,LT2); 1 1 0.697 1 1 1 0.6291 0.8808 50.473 ((ATCC9150,ch),(CT18,Ty2),LT2); 0.525 1 0.101 0.532869 0.941 1 0.0135 0.1558 45.438 (ATCC9150,(ch,(CT18,Ty2)),LT2); 1 1 0.339 0.860028 0.979 1 0.1989 0.5525 53.012 ((ATCC9150,(CT18,Ty2)),ch,LT2); 0.067 0.635187 0.72 1 0.454 1 0.5296 0.8173 47.339 ((ATCC9150,(CT18,Ty2)),ch,LT2); 1 1 0.106 0.545116 1 1 0.3993 0.7305 53.178 ((ATCC9150,ch),(CT18,Ty2),LT2); 0.657 1 0.278 0.788867 1 1 0.626 0.8808 48.212 ((ATCC9150,ch),(CT18,Ty2),LT2); 0.086 0.724894 0.036 0.32457 0.364 1 0.0842 0.3837 53.916 ((ATCC9150,ch),(CT18,Ty2),LT2); 1 1 0.237 0.759308 1 1 0.172 0.5207 57.767 ((ATCC9150,(CT18,Ty2)),ch,LT2); 0.022 0.346287 0.262 0.77271 0.129 1 0.0535 0.3147 47.563

((ATCC9150,(CT18,Ty2)),ch,LT2); 0.444 1 0.23 0.74888 0.243 1 0.147 0.4823 46.408 ((ATCC9150,(CT18,Ty2)),ch,LT2); 0.695 1 0.325 0.840986 0.972 1 0.5762 0.8477 48.278 ((ATCC9150,ch),(CT18,Ty2),LT2); 1 1 0.321 0.836715 1 1 0.6705 0.8934 43.449 ((ATCC9150,(CT18,Ty2)),ch,LT2); 0.123 0.873883 0.752 1 0.352 1 0.4576 0.7687 42.87 ((ATCC9150,ch),(CT18,Ty2),LT2); 0.367 1 0.068 0.435353 0.1 1 0.042 0.2802 44.866 ((ATCC9150,(CT18,Ty2)),ch,LT2); 0.675 1 0.075 0.455597 0.597 1 0.1759 0.5236 44.928 (((ATCC9150,ch),CT18),LT2,Ty2); 1 1 0.172 0.665654 0.506 1 0.0245 0.2080 50.312 (((ATCC9150,ch),LT2),CT18,Ty2); 0.211 1 0.637 1 0.186 1 0.0142 0.1585 43.691 (((ATCC9150,ch),CT18),LT2,Ty2); 1 1 0.418 0.942154 1 1 0.0332 0.2517 41.177 (((ATCC9150,ch),CT18),LT2,Ty2); 1 1 0.817 1 1 1 0.0978 0.4140 47.39 (((ATCC9150,ch),CT18),LT2,Ty2); 0.326 1 0.451 0.969004 0.323 1 0.0786 0.3756 33.909 (((ATCC9150,ch),CT18),LT2,Ty2); 1 1 0.348 0.870458 1 1 0.114 0.4324 48.505 (((ATCC9150,LT2),ch),CT18,Ty2); ND ND ND ND ND ND 0.5617 0.8368 55.467 ((ATCC9150,(ch,LT2)),CT18,Ty2); 0.478 1 0.115 0.56131 0.551 1 0.2734 0.6504 47.143 (((ATCC9150,ch),CT18),LT2,Ty2); 1 1 0.858 1 1 1 0.5067 0.8040 43.944 (((ATCC9150,ch),CT18),LT2,Ty2); ND ND ND ND ND ND 0.5016 0.8008 43.767 (((ATCC9150,ch),CT18),LT2,Ty2); 1 1 0.054 0.39651 1 1 0.0362 0.2619 46.598 (((ATCC9150,ch),CT18),LT2,Ty2); 0.221 1 0.315 0.830958 0.053 1 0.026 0.2148 36.194 (((ATCC9150,ch),LT2),CT18,Ty2); 0.074 0.658831 0.394 0.923873 0.053 1 0.2059 0.5552 40.58 ((ATCC9150,(ch,LT2)),CT18,Ty2); 1 1 0.267 0.777 1 1 1.0000 47.047 ((ATCC9150,(ch,LT2)),CT18,Ty2); ND ND ND ND ND ND 1 1.0000 48.47 (((ATCC9150,LT2),ch),CT18,Ty2); ND ND ND ND ND ND 0.09 0.3968 55.514

Nc value for Choleraesuis i Nc value for Typhi CT18 i Nc value for Typhimurium LT2 i Nc value for Typhi Ty2 i Average Nc i 50.305 49.368 49.611 49.264 49.7836 41.207 41.816 40.998 41.512 41.1402 42.195 48.207 42.403 48.207 44.5578 42.963 43.374 43.356 43.374 43.0602 46.98 50.605 47.235 50.605 48.07 39.518 39.712 39.518 39.712 39.997 46.522 44.789 46.037 44.789 45.5194 42.774 42.417 42.894 42.385 42.5858 37.439 39.877 37.641 39.877 38.8574 45.791 45.092 46.195 45.092 46.3056 49.958 51.793 50.313 51.793 50.866 44.335 44.775 44.084 44.775 44.6814 53.71 52.094 52.232 52.094 52.6284 47.216 47.922 47.831 47.87 47.6356 51.095 59.702 53.111 59.702 55.3576 48.133 47.428 47.848 47.428 47.8098 53.524 53.49 53.752 53.49 53.6344 54.646 50.286 52.073 50.286 53.0116 47.914 47.221 47.827 47.221 47.5492

46.268 46.375 46.178 46.375 46.3208 50.433 48.262 48.083 48.262 48.6636 43.668 43.994 44.313 43.994 43.8836 42.607 42.961 42.549 42.961 42.7896 45.584 45.627 44.902 45.627 45.3212 49.526 45.981 44.686 45.981 46.2204 51.848 51.519 53.53 51.519 51.7456 43.648 43.201 43.268 43.021 43.3658 40.893 40.344 40.618 40.479 40.7022 46.942 47.467 47.71 47.467 47.3952 35.144 33.788 33.919 33.788 34.1096 51.358 48.505 49.535 48.505 49.2816 51.544 51.419 53.85 51.419 52.7398 49.431 48.377 49.106 48.377 48.4868 43.897 44.527 44.906 44.527 44.3602 44.618 44.618 47.566 44.618 45.0374 48.219 49.176 48.706 49.176 48.375 36.796 36.352 36.307 36.352 36.4002 40.883 41.284 41.381 41.284 41.0824 47.566 48.114 47.761 48.114 47.7204 46.368 47.732 48.394 47.732 47.7392 52.746 52.746 50.674 52.746 52.8852