MEBAK Raw Materials Table of Contents

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1 Table of Contents Disclaimer... 3 Preface to the New Edition... 5 Information about the MEBAK website... 6 Information regarding the purchase of MEBAK volumes... 6 Contributors... 7 ADJUNCTS R [ ] Sampling Techniques R [ ] Moisture (EBC-Method) Extract R [ ] Extract DE CLERCK Method (EBC-Method) R [ ] Extract ASBC Method (EBC-Method) R [ ] Protein R [ ] Lipid Content (free crude fat) (EBC-Method) R [ ] Colour of Liquid Adjuncts (EBC-Method) R [ ] Gelatinisation Temperature for Adjuncts Viscometric Method R [ ] Extract Enzymatic Method for Maize (EBC-Method) R [ ] Extract ASBC Method for Liquid Adjuncts (EBC-Method)

2 BARLEY R [ ] Sampling techniques Visual Inspection R [ ] Odour R [ ] Moisture R [ ] Colour and lustre R [ ] Husk quality R [ ] Degree of contamination (Purity) R [ ] Pests R [ ] Grain Anomalies R [ ] Split grains R [ ] Split Grains Visual Inspection R [ ] Split Grains Detection using Iodine-Starch Reaction R [ ] Incomplete Lateral Husk Closure R [ ] Husk Damage R [ ] Immature second growth grains R [ ] Pre-germination R [ ] Mycological Status R [ ] Detection of Fusarium graminearum infection R [ ] Detection of Fusarium culmorum infection R [ ] Ergot R [ ] Size and shape of the grains Varietal Purity R [ ] Varietal purity HCl-Test Physical Tests R [ ] Sieving test (EBC-Method) R [ ] Thousand Grain Weight (EBC-Method) R [ ] Test Weight (Hectolitre weight) Physiological Tests Germinative capacity R [ ] Germinative capacity Staining Method (EBC-Method) R [ ] Germinative Capacity Hydrogen Peroxide Method (EBC-Method) Germinative Energy R [ ] Germinative Energy AUBRY Method

3 R [ ] Germinative Energy SCHÖNFELD Method (EBC-Method) R [ ] Germinative Energy Modified SCHÖNFELD Method R [ ] Germinative Energy BRF Method R [ ] Germinative Energy Germination Percentage and Germination Index (EBC-Method) R [ ] Water Sensitivity R [ ] Water Uptake Capacity (Swelling Capacity) Pre-germination (Number of Germinated Grains) R [ ] Pre-germination Copper Sulphate Method R [ ] Pre-germination Boiling Method R [ ] Pre-germination Fluorescein Dibutyrate Method (EBC-Method) Chemical-Technical Analyses Moisture R [ ] Moisture Drying Oven Method (EBC-Method) R [ ] Moisture Infrared Drying R [ ] Moisture Microwave Drying R [ ] Moisture Conductivity Measurement R [ ] Moisture Capacitance R [ ] Moisture Near Infrared Reflection Spectroscopy (NIR) R [ ] Moisture Near Infrared Transmission Spectroscopy (NIT) Nitrogen (Crude Protein) R [ ] Nitrogen KJELDAHL Method (EBC-Method) R [ ] Nitrogen DUMAS Combustion Method (EBC-Method) R [ ] Protein Near Infrared Reflection R [ ] Protein Near Infrared Transmission Spectroscopy (NIT) (EBC-Method) R [ ] Husk Content (EBC-Method) High Molecular Weight β-glucan R [ ] β-glucan Fluorimetric Method (EBC-Method) R [ ] β-glucan Fluorimetric Method (MTP-Format) R [ ] Varietal Identification using Electrophoresis (EBC-Method) R [ ] Micro-malting

4 MALT Barley Malt R [ ] Sampling Techniques R [ ] Visual Inspection R [ ] Odour R [ ] Flavour and Aroma R [ ] Colour and Lustre R [ ] Degree of Contamination (Purity) R [ ] Size and Shape of the Grains R [ ] Mycological Status Mechanical and physiological Tests R [ ] Sieving R [ ] Thousand Grain Weight (EBC-Method) R [ ] Test Weight (Hectolitre Weight, HW) R [ ] Sinker Test R [ ] Endosperm characteristics glassiness R [ ] Endosperm characteristics colour Friability R [ ] Friabilimeter (EBC-Method) R [ ] Acrospire Development R [ ] Malt Modification and Homogeneity (Calcofluor Carlsberg Method EBC) R [ ] Germinative Capacity Chemical-Technical Analyses R [ ] Moisture (EBC-Method) Total Nitrogen R [ ] Total Nitrogen KJELDAHL Method (EBC-Method) 149 R [ ] Total nitrogen DUMAS combustion method (EBC-Method) R [ ] Protein Near Infrared Reflection Spectroscopy (NIR) R [ ] Protein Near Infrared Transmission Spectroscopy (NIT) R [ ] β-amylase Photometric Method R [ ] Diastatic Power (EBC-Method) α-amylase activity R [ ] α-amylase Photometric method R [ ] α-amylase Viscometric Method (Reference method)

5 R [ ] α-amylase International Method (EBC-Method) 161 β-glucanase Activity R [ ] β-glucanase Photometric Method R [ ] β-glucanase Viscometric Method High Molecular Weight β-glucan R [ ] β-glucan (Malt) Fluorimetric Method (EBC-Method) R [ ] β-glucan (Malt) Fluorimetric Method (MTP Method) R [ ] Steam-Volatile Phenols for the determination of substances which cause a smoky flavour R [ ] Varietal Identification using Electrophoresis R [ ] Dimethyl sulphide (DMS) and its Precursors in Malt R [ ] Nitrosamines in Malt Gushing R [ ] Modified Carlsberg Test R [ ] Rapid Gushing Test for Malt and Adjuncts R [ ] Gelatinisation Temperature (Malt) Viscometric Method R [ ] Iodine Value of Laboratory Spent Grains Wort Production R [ ] Milling (EBC-Method) R [ ] Congress Mash Procedure R [ ] Isothermal 65 C Mash Procedure Wort Analysis R [ ] Extract (EBC-Method) R [ ] Odour of the Mash (EBC-Method) R [ ] Iodine Normality/Saccharification time (EBC-Method) R [ ] Filtration (EBC-Method) R [ ] Appearance R [ ] ph Value (EBC-Method) Wort Colour/Boiled Wort Colour R [ ] Spectrophotometric Colour Measurement (EBC-Method) R [ ] Visual Colour Determination (EBC-Method) R [ ] Boiled Wort Colour (EBC-Method) R [ ] Total Extract Yield Procedure Viscosity (EBC-Method) (for Congress Wort, 65 C Mash)

6 R [ ] Viscosity HÖPPLER Falling Ball Viscometer R [ ] Viscosity Microviscometer from Anton Paar R [ ] Viscosity Rotational Viscometer R [ ] Viscosity Capillary Viscometer Soluble Nitrogen R [ ] Soluble Nitrogen KJELDAL Method (EBC-Method ) R [ ] Soluble Nitrogen DUMAS Combustion Method (EBC-Method) R [ ] Soluble Nitrogen Spectrophotometric method (EBC-Method) R [ ] Ratio of Soluble to Total Nitrogen KOLBACH Index (EBC-Method) R [ ] Nitrogen Fractionation Phosphomolybdic Acid Precipitation Low Molecular Weight Nitrogenous Compounds R [ ] Free Amino Nitrogen (FAN) (EBC-Method) R [ ] Amino Acids in Wort High Molecular Weight β-glucan R [ ] β-glucan (Wort) Colorimetric Method (EBC-Method) R [ ] β-glucan (Wort) Fluorimetric Method (EBC-Method) R [ ] β-glucan (Wort) Fluorimetric Method (MTP Method) Fermentable Carbohydrates in Congress Wort Limit of Attenuation R [ ] Limit of Attenuation in Congress Wort (Fermentation Tube Method) R [ ] Limit of Attenuation in Congress Wort (Reference Method EBC-Method) R [ ] Limit of Attenuation in Congress Wort (Rapid Method EBC-Method) R [ ] Fermentable Sugars by HPLC R [ ] Fermentable Carbohydrates by HPLC (EBC-Method) R [ ] Thiobarbituric Acid Index (TBI) R [ ] Organic Acids in Congress Wort Atomic Absorption Spectroscopy Minerals R [ ] Iron R [ ] Sodium

7 R [ ] Potassium R [ ] Calcium (EBC-Method) R [ ] Manganese R [ ] Zinc (EBC-Method) R [ ] Copper R [ ] Magnesium R [ ] Aluminium R [ ] Tin R [ ] Metals detection by Atomic Absorption Spectroscopy using Inductively Coupled Plasma (ICP-AES or ICP-OES) Wheat Malts Special Malts R [ ] Moisture (EBC-Method) R [ ] Extract in Roasted and Caramel Malt (EBC-Method) R [ ] Colour in Roasted and Caramel Malt (EBC-Method) R [ ] ph-value (Sour Malt) R [ ] Titration Acidity (Sour Malt) R [ ] L-Lactic Acid (EBC-Method)/ D-Lactic Acid (Lactate) R [ ] Colour of Liquid Roasted Malt Extract Spectrophotometric Method R [ ] Colour of Liquid Roasted Malt Extract Visual Method

8 RAW HOPS AND HOP PRODUCTS R [ ] Sampling techniques R [ ] Sample preparation R [ ] Moisture (EBC-Method) Bitter Substances R [ ] Bitter Substances in Hops and Hop Products: Lead Conductance Value and Total Resin, Soft Resin and Hard Resin (EBC-Method) R [ ] α- and β-acids by Spectrophotometry (ASBC Method) R [ ] α- and β-acids by HPLC (EBC-Method) Aroma Components (Hop essential oils) R [ ] Volumetric Hop Oil Analysis (EBC-Method) R [ ] Hop Essential Oil Components R [ ] Four selected Hop Essential Oil Components (EBC-Method) R [ ] Total Polyphenols Spectrophotometric (EBC-Method) Ageing components R [ ] Proportion of Hard Resins R [ ] Hop Storage Index, HSI (EBC-Method) Raw Hops and Hop Pellets R [ ] Hand evaluation of the raw hops R [ ] Picking Quality R [ ] Colour and Lustre R [ ] Cone structure R [ ] Aroma R [ ] α-acids R [ ] Moisture content R [ ] Diseases, Pests and Seeds R [ ] Improper Handling R [ ] Overall Evaluation Isomerised Pellets R [ ] Sample Preparation R [ ] Sampling Techniques Bitter Substances R [ ] iso-α-, α- and β-acids in Isomerised Pellets (EBC-Method)

9 Hop Extract R [ ] Sample Preparation R [ ] Sampling Techniques R [ ] Moisture (Karl-Fischer-Method) Bitter substances R [ ] Bitter Substances in Hop Extract: Lead Conductance Value and Total Resin, Soft Resin and Hard Resin (EBC-Method) R [ ] α- and β-acids by HPLC (EBC-Method) Isomerised Hop Extract R [ ] Sample Preparation R [ ] Sampling Techniques Bitter Substances R [ ] iso-α-, α- and β-acids in Hop Extracts and Isomerised Hop Extracts (EBC-Method) R [ ] iso-α-acids and Reduced iso-α-acids in Hop Products Subject Index List of Figures List of Tables List of Advertisers

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1.4.2 Germinative Energy Germinative Energy AUBRY Method (EBC) Germinative Energy SCHÖNFELD Method (EBC) Table of Contents Preface to the New Edition... 3 Information about the MEBAK Website... 4 Contributors... 5 Table of Contents... 7 List of Abbreviations... 16 1 Barley... 14 1.1 Sample Collection... 16