GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime

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1 GeneCopoeia TM Expressway to Discovery ExProfile TM Human Lung Cancer Gene qpcr Array For focused group profiling of human lung cancer related genes expression Cat. No. QG071-A (6 x 96-well plate, Format A) Cat. No. QG071-B (6 x 96-well plate, Format B) Cat. No. QG071-C (6 x 96-well plate, Format C) Cat. No. QG071-D (6 x 96-well plate, Format D) Cat. No. QG071-E (6 x 96-well plate, Format E) Available as 1 set or 6 sets. Each set contains 504 unique gene primers deposited in six 96-well plates. Introduction The ExProfile human lung cancer gene qpcr array profiles 504 human genes to aberrantly expressed human genes involved in human lung cancer. These genes are carefully chosen for their close cancer correlation based on a thorough literature search of peer-reviewed publications. Abnormal gene expression is often observed in cancer development and progression. The ExPofile human lung caner gene array allows researchers to study the cancer-related genes to gain understanding of their roles of genes in head and lung cancer pathogenesis. QG071 plate 01: 84 unique gene PCR primer pairs QG071 plate 02: 84 unique gene PCR primer pairs QG071 plate 03: 84 unique gene PCR primer pairs QG071 plate 04: 84 unique gene PCR primer pairs QG071 plate 05: 84 unique gene PCR primer pairs QG071 plate 06: 84 unique gene PCR primer pairs Shipping and storage condition Shipped at room temperate Stable for at least 6 months when stored at -20 C Array format GeneCopoeia provides five qpcr array formats (A, B, C, D, and E) suitable for use with the following realtime cyclers. Important note: Upon receiving, please check to make sure that the correct array format was ordered to ensure the compatibility with your qpcr instrument. Plate format Instrument provider qpcr instrument model A (96-well) B (96-well) C (96-well) D (96-well) E (96-well) Applied Biosystems Applied Biosystems Bio-Rad Laboratories Bio-Rad Laboratories Roche Applied Science 5700, 7000, 7300, 7500, 7700, 7900HT (Standard 96-well block), ViiA TM 7 (Standard 96-well block) 7500 (Fast block), 7900HT (Fast block), StepOnePlus TM, ViiA TM 7(Fastblock) icycler iq,myiq,iq 5 CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 LightCycler 480 (96-well block) 1

2 Quality control 1. Each pair of primers in the ExProfile gene qpcr array has been experimentally validated to yield a single dissociation curve peak and to generate a single amplicon of the correct size for the targeted gene. 2. The positive PCR controls (PCR) have been verified to amplify a single amplicon of the correct size with Ct values around 20±2. 3. The Spike-in reverse transcription controls (RT) have been verified to amplify a single amplicon of the correct size with Ct values around 20±3. 4. R 2 > 0.99 was observed for high inter/ intra-array reproducibility. Materials required but not provided All-in-One TM First-Strand cdna Synthesis Kit All-in-One TM qpcr Mix Total RNA extraction kit (RNAzol RT RNA extraction reagent is recommended) DNase/RNase free tips, PCR reaction tubes, 1.5 ml microcentrifuge tubes 5 ml and 10 ml graduated pipettes, beakers, flasks, and cylinders 10 μl to1,000μl adjustable single channel micropipettes with disposable tips 5 μl to20μl adjustable multichannel micropipette, disposable tips, and reservoir qpcr instrument, compatible with gene qpcr arrays ordered Array layout Figure1. Illustration of QG071 plate 01 Figure2. Illustration of QG071 plate 02 Figure3. Illustration of QG071 plate 03 2

3 Figure4. Illustration of QG071 plate 04 Figure5. Illustration of QG071 plate 05 Figure6. Illustration of QG071 plate 06 Gene primer pairs: 84 wells (A row to G row) are designated for a real-time PCR assay for genes (see the primer list). HK1-6: Six pre-deposited housekeeping gene (HK1-6) primer pairs, which can be used as endogenous positive controls as well as for array normalization. GDC: Genomic DNA controls, which can be used to specifically detect genomic DNA contamination with a high level of sensitivity. RT: Spike-in reverse transcription controls, which can be used to monitor the efficiency of the RT reactions. These pre-deposited primer pairs specifically amplify the cdna template reversed transcribed from the spike-in control RNA in the sample. PCR: Positive PCR controls, which are used to verify the PCR efficiency by amplifying the predeposited DNA template with its specific pre-deposited primer pairs. Gene primer list Plate Position Catalog No. of Primer Accession No. of Gene Symbol QG A01 HQP NM_ EGFR QG A02 HQP NM_ TP53 QG A03 HQP NM_ CYP1A1 QG A04 HQP NM_ KRAS QG A05 HQP NM_ XRCC1 3

4 4 QG A06 HQP NM_ ERCC2 QG A07 HQP NM_ MPO QG A08 HQP NM_ ERCC1 QG A09 HQP NM_ OGG1 QG A10 HQP NM_ EPHX1 QG A11 HQP NM_ NQO1 QG A12 HQP NM_ NAT2 QG B01 HQP NM_ MTHFR QG B02 HQP NM_ CYP2E1 QG B03 HQP NM_ CHRNA5 QG B04 HQP NM_ XPC QG B05 HQP NM_ MDM2 QG B06 HQP NM_ CHRNA3 QG B07 HQP NM_ TNF QG B08 HQP NM_ APEX1 QG B09 HQP NM_ IL1B QG B10 HQP NM_ MMP1 QG B11 HQP NM_ XRCC3 QG B12 HQP NM_ PTGS2 QG C01 HQP NM_ ABCB1 QG C02 HQP NM_ IL6 QG C03 HQP NM_ CYP1B1 QG C04 HQP NM_ SOD2 QG C05 HQP NM_ ERCC5 QG C06 HQP NM_ GSTM3 QG C07 HQP NM_ VEGFA QG C08 HQP NM_ IL8 QG C09 HQP NM_ TGFB1 QG C10 HQP NM_ IL10 QG C11 HQP NM_ STK11 QG C12 HQP NM_ CCND1 QG D01 HQP NM_ FASLG QG D02 HQP NM_ CYP3A5 QG D03 HQP NM_ CLPTM1L QG D04 HQP NM_ MMP3 QG D05 HQP NM_ ERCC4 QG D06 HQP NM_ CYP2D6 QG D07 HQP NM_ CYP2C19 QG D08 HQP NM_ TP73 QG D09 HQP NM_ XRCC2 QG D10 HQP NM_ NOS3 QG D11 HQP NM_ NBN QG D12 HQP NM_ MLH1 QG E01 HQP NM_ ERCC6 QG E02 HQP NM_ DRD2 QG E03 HQP NM_ TYMS QG E04 HQP NM_ SULT1A1 QG E05 HQP NM_ BRCA2

5 5 QG E06 HQP NM_ PPARG QG E07 HQP NM_ MMP9 QG E08 HQP NM_ MMP2 QG E09 HQP NM_ LIG1 QG E10 HQP NM_ IL13 QG E11 HQP NM_ IL1RN QG E12 HQP NM_ IL1A QG F01 HQP NM_ PARP1 QG F02 HQP NM_ COMT QG F03 HQP NM_ EXO1 QG F04 HQP NM_ NAT1 QG F05 HQP NM_ BAT3 QG F06 HQP NM_ CASP8 QG F07 HQP NM_ XRCC4 QG F08 HQP NM_ BRAF QG F09 HQP NM_ SLC19A1 QG F10 HQP NM_ PTEN QG F11 HQP NM_ PSMA4 QG F12 HQP NM_ MTRR QG G01 HQP NM_ MSH2 QG G02 HQP NM_ MMP12 QG G03 HQP NM_ MET QG G04 HQP NM_ IL4 QG G05 HQP NM_ IGFBP3 QG G06 HQP NM_ HLA-A QG G07 HQP NM_ ALDH2 QG G08 HQP NM_ AHR QG G09 HQP NM_ CYP19A1 QG G10 HQP NM_ CYP17A1 QG G11 HQP NM_ CHRNB4 QG G12 HQP NM_ CHEK2 QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR QG A01 HQP NM_ CDKN2A QG A02 HQP BC TGFBR1 QG A03 HQP NM_ CCNH QG A04 HQP NM_ MBD4 QG A05 HQP NM_ GGH

6 6 QG A06 HQP NM_ TNFRSF10A QG A07 HQP NM_ CBS QG A08 HQP NM_ AKR1C3 QG A09 HQP NM_ CASP3 QG A10 HQP NM_ VDR QG A11 HQP NM_ RAD23B QG A12 HQP NM_ SERPINA1 QG B01 HQP NM_ MTR QG B02 HQP NM_ LIG3 QG B03 HQP NM_ IL12B QG B04 HQP NM_ IL12A QG B05 HQP NM_ IL8RA QG B06 HQP NM_ IL6R QG B07 HQP NM_ IL4R QG B08 HQP NM_ IL2 QG B09 HQP NM_ IFNAR2 QG B10 HQP NM_ GSTA4 QG B11 HQP NM_ AKT1 QG B12 HQP NM_ ACE QG C01 HQP NM_ CYP2C9 QG C02 HQP NM_ LOC QG C03 HQP NM_ MYCL1 QG C04 HQP NM_ RRM1 QG C05 HQP BC HLA-DRB1 QG C06 HQP NM_ CDA QG C07 HQP NM_ XRCC5 QG C08 HQP NM_ BCR QG C09 HQP NM_ POLB QG C10 HQP NM_ PCNA QG C11 HQP NM_ LIG4 QG C12 HQP NM_ IFNGR2 QG D01 HQP NM_ HSD17B1 QG D02 HQP NM_ RGS17 QG D03 HQP NM_ FGFR4 QG D04 HQP NM_ ESR1 QG D05 HQP NM_ DRD4 QG D06 HQP NM_ CYP2A13 QG D07 HQP NM_ CSF2 QG D08 HQP NM_ RASSF1 QG D09 HQP NM_ SOD3 QG D10 HQP BC RET QG D11 HQP NM_ CDH1 QG D12 HQP NM_ CDC25C QG E01 HQP NM_ RGS6 QG E02 HQP NM_ TP53I3 QG E03 HQP NM_ TNKS QG E04 HQP NM_ CASP7 QG E05 HQP NM_ TP53BP1

7 7 QG E06 HQP NM_ TERF2 QG E07 HQP NM_ TERF1 QG E08 HQP NM_ SFTPB QG E09 HQP NM_ NOD2 QG E10 HQP NM_ BHMT QG E11 HQP NM_ CCL2 QG E12 HQP NM_ RAD52 QG F01 HQP NM_ BARD1 QG F02 HQP NM_ ATR QG F03 HQP NM_ PIM1 QG F04 HQP NM_ PIK3CA QG F05 HQP NM_ REV1 QG F06 HQP NM_ NOS2A QG F07 HQP NM_ MYC QG F08 HQP NM_ ABCC1 QG F09 HQP NM_ LRMP QG F10 HQP NM_ LEP QG F11 HQP NM_ IL15RA QG F12 HQP NM_ IL15 QG G01 HQP NM_ IL7R QG G02 HQP NM_ IGF1R QG G03 HQP NM_ IGF1 QG G04 HQP NM_ HSD3B1 QG G05 HQP NM_ APC QG G06 HQP NM_ MSH6 QG G07 HQP NM_ GSTZ1 QG G08 HQP NM_ GSTA2 QG G09 HQP NM_ GPX3 QG G10 HQP NM_ GPX2 QG G11 HQP NM_ POT1 QG G12 HQP NM_ FHIT QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR QG A01 HQP NM_ ERCC3 QG A02 HQP NM_ ELA2 QG A03 HQP NM_ EGF QG A04 HQP NM_ DHFR QG A05 HQP NM_ CYP11A1

8 8 QG A06 HQP NM_ CTLA4 QG A07 HQP NM_ CSF3 QG A08 HQP NM_ SERPINA3 QG A09 HQP NM_ ADH1C QG A10 HQP NM_ POLI QG A11 HQP NM_ CHEK1 QG A12 HQP NM_ ALDH1L1 QG B01 HQP NM_ CDK5 QG B02 HQP NM_ ERBB4 QG B03 HQP NM_ DNMT3B QG B04 HQP NM_ ABCC4 QG B05 HQP NM_ SLC2A4 QG B06 HQP NM_ SHBG QG B07 HQP NM_ NR1H4 QG B08 HQP NM_ SLC23A1 QG B09 HQP NM_ CDC25B QG B10 HQP NM_ CDC25A QG B11 HQP NM_ RB1CC1 QG B12 HQP NM_ MATR3 QG C01 HQP NM_ CD81 QG C02 HQP NM_ CD40 QG C03 HQP NM_ MPDU1 QG C04 HQP NM_ GDF15 QG C05 HQP NM_ LITAF QG C06 HQP NM_ SCARB1 QG C07 HQP NM_ CD86 QG C08 HQP NM_ CYP7B1 QG C09 HQP NM_ CD80 QG C10 HQP NM_ CER1 QG C11 HQP NM_ CD4 QG C12 HQP NM_ SLC28A1 QG D01 HQP NM_ PDCD5 QG D02 HQP NM_ MBD2 QG D03 HQP NM_ CBR3 QG D04 HQP NM_ CBR1 QG D05 HQP NM_ ABCC3 QG D06 HQP NM_ ABCB11 QG D07 HQP NM_ CAV1 QG D08 HQP NM_ RGS5 QG D09 HQP NM_ CASR QG D10 HQP NM_ RAD54L QG D11 HQP NM_ PLA2G6 QG D12 HQP NM_ CASP5 QG E01 HQP NM_ FZD7 QG E02 HQP NM_ AXIN2 QG E03 HQP NM_ NCOA3 QG E04 HQP NM_ COL18A1 QG E05 HQP NM_ CALCR

9 9 QG E06 HQP NM_ ZNF230 QG E07 HQP NM_ XBP1 QG E08 HQP NM_ WRN QG E09 HQP NM_ VIL2 QG E10 HQP NM_ VCAM1 QG E11 HQP NM_ UCP3 QG E12 HQP NM_ TYR QG F01 HQP NM_ TSG101 QG F02 HQP NM_ TNFRSF1B QG F03 HQP NM_ TGM1 QG F04 HQP NM_ TFF1 QG F05 HQP NM_ TEP1 QG F06 HQP NM_ STAT1 QG F07 HQP NM_ SSTR3 QG F08 HQP NM_ SOD1 QG F09 HQP NM_ SLC4A2 QG F10 HQP NM_ SLAMF1 QG F11 HQP NM_ SFTPD QG F12 HQP NM_ ABCG8 QG G01 HQP NM_ BLM QG G02 HQP NM_ SEPP1 QG G03 HQP NM_ SELE QG G04 HQP NM_ CCL5 QG G05 HQP NM_ RXRB QG G06 HQP NM_ RXRA QG G07 HQP NM_ ROS1 QG G08 HQP NM_ RNASEL QG G09 HQP NM_ BCL2 QG G10 HQP NM_ ZNF350 QG G11 HQP NM_ RAC1 QG G12 HQP NM_ PCTP QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR QG A01 HQP NM_ BAK1 QG A02 HQP NM_ PTGS1 QG A03 HQP NM_ RAD18 QG A04 HQP NM_ MYNN QG A05 HQP NM_ HIF1AN

10 10 QG A06 HQP NM_ FBXW7 QG A07 HQP NM_ CASC1 QG A08 HQP NM_ WDR79 QG A09 HQP NM_ UGT1A4 QG A10 HQP NM_ UGT1A7 QG A11 HQP NM_ POLD1 QG A12 HQP NM_ TLR9 QG B01 HQP NM_ PMS2 QG B02 HQP NM_ PMS1 QG B03 HQP NM_ PLK1 QG B04 HQP NM_ PLAUR QG B05 HQP NM_ PLAU QG B06 HQP NM_ PLA2G2A QG B07 HQP NM_ SERPINE2 QG B08 HQP NM_ PHB QG B09 HQP NM_ PGR QG B10 HQP NM_ P2RX7 QG B11 HQP NM_ OPRM1 QG B12 HQP NM_ OCA2 QG C01 HQP NM_ ATP1B2 QG C02 HQP NM_ NINJ1 QG C03 HQP NM_ NFKBIE QG C04 HQP NM_ NFKBIA QG C05 HQP NM_ NFKB1 QG C06 HQP NM_ NCF2 QG C07 HQP NM_ NUBP1 QG C08 HQP NM_ MX1 QG C09 HQP NM_ MSH3 QG C10 HQP NM_ MIF QG C11 HQP NM_ MEST QG C12 HQP NM_ MBD1 QG D01 HQP NM_ MAOA QG D02 HQP NM_ ARNT QG D03 HQP NM_ LRP5 QG D04 HQP NM_ LRP6 QG D05 HQP NM_ LPL QG D06 HQP NM_ LIPC QG D07 HQP NM_ ARHGDIB QG D08 HQP NM_ LEPR QG D09 HQP NM_ LDLR QG D10 HQP NM_ LCAT QG D11 HQP NM_ KDR QG D12 HQP NM_ RAB15 QG E01 HQP NM_ JAK3 QG E02 HQP NM_ ITGB3 QG E03 HQP NM_ IRS1 QG E04 HQP NM_ IRF3 QG E05 HQP NM_ IRF1

11 11 QG E06 HQP NM_ IREB2 QG E07 HQP NM_ INSR QG E08 HQP NM_ IL18 QG E09 HQP NM_ IL12RB1 QG E10 HQP NM_ IL10RB QG E11 HQP NM_ IL2RB QG E12 HQP NM_ IL1R1 QG F01 HQP NM_ APOE QG F02 HQP NM_ IGFBP6 QG F03 HQP NM_ IGFBP5 QG F04 HQP NM_ IGFBP2 QG F05 HQP NM_ IGFBP1 QG F06 HQP NM_ IGF2 QG F07 HQP NM_ IFNGR1 QG F08 HQP NM_ IFNAR1 QG F09 HQP NM_ APOA4 QG F10 HQP NM_ APOA2 QG F11 HQP NM_ HUS1 QG F12 HQP NM_ HTR1D QG G01 HQP NM_ HTR1B QG G02 HQP NM_ BIRC5 QG G03 HQP NM_ BIRC2 QG G04 HQP NM_ HSD17B4 QG G05 HQP NM_ HSD17B2 QG G06 HQP NM_ HSD3B2 QG G07 HQP NM_ APAF1 QG G08 HQP NM_ HMGCR QG G09 HQP NM_ HIF1A QG G10 HQP NM_ HFE QG G11 HQP NM_ HADHA QG G12 HQP NM_ SLC39A2 QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR QG A01 HQP NM_ RPA4 QG A02 HQP NM_ GSK3B QG A03 HQP NM_ GRPR QG A04 HQP NM_ GPX4 QG A05 HQP NM_ AMT

12 12 QG A06 HQP NM_ DHDH QG A07 HQP NM_ GC QG A08 HQP NM_ HSPB8 QG A09 HQP NM_ KRT23 QG A10 HQP NM_ LMOD1 QG A11 HQP NM_ XRCC6 QG A12 HQP NM_ ALOX15 QG B01 HQP NM_ ALOX5 QG B02 HQP NM_ ALOX12 QG B03 HQP NM_ AMACR QG B04 HQP NM_ FPGS QG B05 HQP NM_ SEZ6L QG B06 HQP NM_ SEC14L2 QG B07 HQP NM_ FOS QG B08 HQP NM_ ABCA5 QG B09 HQP NM_ ABCA6 QG B10 HQP NM_ FOXC1 QG B11 HQP NM_ ITGA11 QG B12 HQP NM_ GPC5 QG C01 HQP NM_ FANCA QG C02 HQP NM_ ESR2 QG C03 HQP NM_ AKT2 QG C04 HQP NM_ EPHX2 QG C05 HQP NM_ DNAJC18 QG C06 HQP NM_ ABCA1 QG C07 HQP NM_ EFNB3 QG C08 HQP NM_ EDN1 QG C09 HQP NM_ DRD1 QG C10 HQP NM_ CYP27B1 QG C11 HQP NM_ CYP24A1 QG C12 HQP NM_ CYP2F1 QG D01 HQP NM_ CYBB QG D02 HQP NM_ CX3CR1 QG D03 HQP NM_ CTSH QG D04 HQP NM_ CTNNB1 QG D05 HQP NM_ CTH QG D06 HQP NM_ CSTF1 QG D07 HQP NM_ PARP4 QG D08 HQP NM_ CSF1R QG D09 HQP NM_ CCR5 QG D10 HQP NM_ SAT2 QG D11 HQP NM_ AKR1C4 QG D12 HQP NM_ CGA QG E01 HQP NM_ MTHFD2 QG E02 HQP NM_ CETP QG E03 HQP NM_ SLCO1B1 QG E04 HQP NM_ AKR1A1 QG E05 HQP NM_ CDKN1C

13 13 QG E06 HQP NM_ CDK7 QG E07 HQP NM_ CDK4 QG E08 HQP NM_ AKAP9 QG E09 HQP NM_ NUBP2 QG E10 HQP NM_ PTH QG E11 HQP NM_ PAK6 QG E12 HQP NM_ MASP1 QG F01 HQP NM_ OPRD1 QG F02 HQP NM_ LMO2 QG F03 HQP NM_ IGFALS QG F04 HQP NM_ ALAD QG F05 HQP NM_ FAM82A QG F06 HQP NM_ TNIP1 QG F07 HQP NM_ LOC QG F08 HQP NM_ SUMO1 QG F09 HQP NM_ KLF6 QG F10 HQP NM_ CDC2 QG F11 HQP NM_ KEAP1 QG F12 HQP NM_ HDAC9 QG G01 HQP NM_ GSTO1 QG G02 HQP NM_ SEP15 QG G03 HQP NM_ CD14 QG G04 HQP NM_ DDX18 QG G05 HQP NM_ NR1I2 QG G06 HQP NM_ CFLAR QG G07 HQP NM_ EIF3S3 QG G08 HQP NM_ MFSD2 QG G09 HQP NM_ HAVCR2 QG G10 HQP NM_ CASP6 QG G11 HQP NM_ MAD1L1 QG G12 HQP NM_ TFPI2 QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR QG A01 HQP NM_ SUV39H2 QG A02 HQP NM_ NEIL1 QG A03 HQP NM_ BHLHB3 QG A04 HQP NM_ FTO QG A05 HQP NM_ SEMA3B

14 14 QG A06 HQP NM_ PRDM2 QG A07 HQP NM_ SLC30A4 QG A08 HQP NM_ SLC30A2 QG A09 HQP NM_ SCGB1A1 QG A10 HQP NM_ UBE2I QG A11 HQP NM_ TWIST1 QG A12 HQP NM_ C5AR1 QG B01 HQP NM_ BUB1B QG B02 HQP NM_ TDG QG B03 HQP NM_ STAT3 QG B04 HQP NM_ SLC22A3 QG B05 HQP NM_ SLC30A5 QG B06 HQP NM_ CYP3A43 QG B07 HQP NM_ MAP2K4 QG B08 HQP NM_ CXCL12 QG B09 HQP NM_ SAT1 QG B10 HQP NM_ S100A2 QG B11 HQP NM_ RGS2 QG B12 HQP NM_ RFC1 QG C01 HQP NM_ RB1 QG C02 HQP NM_ RASA1 QG C03 HQP NM_ RAGE QG C04 HQP NM_ PXN QG C05 HQP NM_ PTPRJ QG C06 HQP NM_ PTPN13 QG C07 HQP NM_ CYP4F11 QG C08 HQP NM_ XPO5 QG C09 HQP NM_ PTGIS QG C10 HQP NM_ PTGER2 QG C11 HQP NM_ MAP2K1 QG C12 HQP NM_ APOM QG D01 HQP NM_ LAPTM4B QG D02 HQP NM_ PPP2R1B QG D03 HQP NM_ TRIT1 QG D04 HQP NM_ PPARD QG D05 HQP NM_ PPARA QG D06 HQP NM_ PON1 QG D07 HQP NM_ TERF2IP QG D08 HQP NM_ POLE QG D09 HQP NM_ HSPA14 QG D10 HQP NM_ SH3GLB1 QG D11 HQP NM_ GEMIN4 QG D12 HQP NM_ SERPINB2 QG E01 HQP NM_ SERPINE1 QG E02 HQP NM_ ATP2A3 QG E03 HQP NM_ NRAS QG E04 HQP NM_ NME1 QG E05 HQP NM_ NFE2L2

15 QG E06 HQP NM_ PPP1R12A QG E07 HQP NM_ MUC1 QG E08 HQP NM_ MSRA QG E09 HQP NM_ MPG QG E10 HQP NM_ MNAT1 QG E11 HQP NM_ MMP8 QG E12 HQP NM_ MLLT3 QG F01 HQP NM_ ARG2 QG F02 HQP NM_ ARG1 QG F03 HQP NM_ KIT QG F04 HQP NM_ ITGB8 QG F05 HQP NM_ AR QG F06 HQP NM_ IL8RB QG F07 HQP NM_ IL5RA QG F08 HQP NM_ IL5 QG F09 HQP NM_ IL1RAP QG F10 HQP NM_ IDH2 QG F11 HQP NM_ IDH1 QG F12 HQP NM_ APOB QG G01 HQP NM_ BIRC4 QG G02 HQP NM_ HSPB1 QG G03 HQP NM_ HSPA8 QG G04 HQP NM_ HRAS QG G05 HQP NM_ HPRT1 QG G06 HQP NM_ HPGD QG G07 HQP NM_ CARD10 QG G08 HQP NM_ GTF2H1 QG G09 HQP NM_ GSTM2 QG G10 HQP NM_ GSTA3 QG G11 HQP NM_ GSS QG G12 HQP NM_ GSR QG H01 HGDC QG H02 HGDC QG H03 HQP NM_ GAPDH QG H04 HQP NM_ ACTB QG H05 HQP NM_ B2M QG H06 HQP NM_ RPL13A QG H07 HQP NM_ HPRT1 QG H08 HQP NR_ RN18S1 QG H09 RT QG H10 RT QG H11 PCR QG H12 PCR 15

16 Limited Use License Following terms and conditions apply to use of ExProfile TM Lung Cancer Gene qpcr Array (the Product). If the terms and conditions are not acceptable, the Product in its entirety must be returned to GeneCopoeia within 5 calendar days. A limited End- User license is granted to the purchaser of the Product. The Product shall be used by the purchaser for internal research purposes only. The Product is expressly not designed, intended, or warranted for use in humans or for therapeutic or diagnostic use. The Product must not be resold, repackaged or modified for resale, or used to manufacture commercial products or deliver information obtained in service without prior written consent from GeneCopoeia. This Product should be used in accordance with the NIH guidelines developed for recombinant DNA and genetic research. Use of any part of the Product constitutes acceptance of the above terms. Limited Warranty GeneCopoeia warrants that the Product meets the specifications described in the accompanying Product Datasheet. If it is proven to the satisfaction of GeneCopoeia that the Product fails to meet these specifications, GeneCopoeia will replace the Product. In the event a replacement cannot be provided, GeneCopoeia will provide the purchaser with a refund. This limited warranty shall not extend to anyone other than the original purchaser of the Product. Notice of nonconforming products must be made to GeneCopoeia within 30 days of receipt of the Product. GeneCopoeia s liability is expressly limited to replacement of Product or a refund limited to the actual purchase price. GeneCopoeia s liability does not extend to any damages arising from use or improper use of the Product, or losses associated with the use of additional materials or reagents. This limited warranty is the sole and exclusive warranty. GeneCopoeia does not provide any other warranties of any kind, expressed or implied, including the merchantability or fitness of the Product for a particular purpose. GeneCopoeia is committed to providing our customers with high-quality products. If you should have any questions or concerns about any GeneCopoeia products, please contact us at GeneCopoeia, Inc. GeneCopoeia, Inc Medical Center Drive, Suite 101 Rockville, MD (301) (866) inquiry@genecopoeia.com GeneCopoeia Products are for Research Use Only Copyright 2016 GeneCopoeia, Inc. Trademarks: GeneCopoeia, ExProfile, All-in-One, (GeneCopoeia Inc.); RNAzol RT (Molecular Research Center, Inc.); SYBR (Molecular Probes); icycler iq, MyiQ, iq 5, CFX96, DNA Engine Opticon, DNA Engine Opticon 2, Chromo4 (Bio-Rad); LightCycler (Roche);Trizol, ABI,ROX, ViiA TM,StepOnePlus TM (Life Technologies). QG