Progress in fabrication of porous silk scaffolds and its application

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1 Vol. 20, No. 1 Feb., (2008) (, ) TS102.33; R A Progress in fabrication of porous silk scaffolds and its application LU Bao-yong, LI Min* (College of Life Science, Fujian Normal University, Fuzhou , China) Abstract: Silk fibres and silk-based materials such as spider and mulberry silkworm silk fibres have been widely used in tissue engineering and biomedical application due to its good biocompatibility. The content of this review is to discuss the recent progress in the research on the fabrication of silk scaffold, cellular compatibility in vitro as well as the implantation in vivo with silk fibres. Key words: tissue engineering; silk scaffold; cell culture; biocompatibility [1] 1 Hakimi [2] (Bombyx mori silkworm) [3-4] ( ) ( ); ( ) * mli@fjnu.edu.cn

2 [5] 1 ( ) 2 1 [6] (MPa): (Araneus MA silk) (B. mori) (MPa): (Araneus MA silk) (B. mori) B. mori [7-9] [10-12] 2.1 NaCl Marolt [13] 9.3M LiBr 17%(w/v) NaCl =1 20 NaCl ( mm) NaCl 95.5% 2.2, Li [14] (PEO) ( 2 BMP-2 ( ) nhap) PEO nm nm [15] 3

3 1 155 [16] 3.1 [17] Hofmann [18] 10% 24 h 5 µm - (vwf) Weibel-palade vwf vwf [19] 3.3 (SEM) (TEM) Gil [20] (SF) (G) 200 m (MeOH) 200 min SF G β 24h G/SF SF G G/SF SF MeOH SF β X CT MRI X CT MRI CT MRI [13] CT MRI 4 [21] (ISO) (GB/T) 16886

4 [1] Meinel [22] RGD(Arg-Gly- Asp) 6 RGD PLA Dal [19] Gobin [23] (SFCS) SFCS (HADM) SFCS SFCS SFCS 5 [24] [1]. [M]. :, 2002: 1-3, [2] Hakimi O, Knigh D P, Vollrath F, et al. Spider and mulberry silkworm silks as compatible biomaterials. Composites Part B: Engineering, 2007, 38(3): [3] Allmeling C, Jokuszies A, Reimers K, et al. Use of spider silk fibres as an innovative material in a biocompatible artificial nerve conduit. J Cell Mol Med, 2006, 10(3):770-7 [4] Huang J, Wong C, George A, et al. The effect of genetically engineered spider silk-dentin matrix protein 1 chimeric protein on hydroxyapatite nucleation. Biomaterials, 2007, 28 (14): [5] Sheu HS, Phyu KW, Jean YC, et al. Lattice deformation and thermal stability of crystals in spider silk. Int J Biol Macromol, 2004, 34(5): [6] Gosline JM, Guerette PA, Ortlepp CS, et al. The mechanical design of spider silks: from fibroin sequence to mechanical function. J Exp Biol, 1999, 202(Pt 23): [7],.., 2002, 18(3): [8],,,. RGD-., 2004, 21(6): [9] Fahnestock SR, Bedzyk LA. Production of synthetic spider dragline silk protein in Pichia pastoris. Appl Microbiol Biotechnol, 1997, 47(1): 33-9 [10],,.., 2006, 6(3): 1-5 [11],,,.., 2003, 25(3): [12],.., 2003, 16(1): 91-6 [13] Marolt D, Augst A, Freed LE, et al. Bone and cartilage tissue constructs grown using human bone marrow stromal cells, silk scaffolds and rotating bioreactors. Biomaterials, 2006, 27(36): [14] Li C, Vepari C, Jin HJ, et al. Electrospun silk-bmp-2 scaffolds for bone tissue engineering. Biomaterials, 2006, 27 (16): [15] Chen DL, Li M, Huang X, et al. Electrospinning and morphology of recombinant spider silk protein nanofibers [C]//

5 th East-Asian Polymer Conference. :, 2006: [16],,,. [C]//. :, 2006: 405 [17] Figallo E, Flaibani M, Zavan B, et al. Micropatterned biopolymer 3D scaffold for static and dynamic culture of human fibroblasts. Biotechnol Prog, 2007, 23(1): [18] Hofmann S, Hagenmuller H, Koch AM, et al. Control of in vitro tissue-engineered bone-like structures using human mesenchymal stem cells and porous silk scaffolds. Biomaterials, 2007, 28(6): [19] Dal Pra I, Freddi G, Minic J, et al. De novo engineering of reticular connective tissue in vivo by silk fibroin nonwoven materials. Biomaterials, 2005, 26(14): [20] Gil ES, Frankowski DJ, Hudson SM, et al. Silk fibroin membranes from solvent-crystallized silk fibroin/gelatin blends: Effects of blend and solvent composition. Materials Sci Engineering: C, 2007, 27(3): [21]. [M]. :, 2004: 494 [22] Meinel L, Hofmann S, Karageorgiou V, et al. The inflammatory responses to silk films in vitro and in vivo. Biomaterials, 2005, 26(2): [23] Gobin AS, Butler CE, Mathur AB. Repair and regeneration of the abdominal wall musculofascial defect using Silk fibroin-chitosan blend. Tissue Eng, 2006, 12 (2): [24],.., 2006, 29(5): (Bimonthly) ( )(1988 ) (Started in 1988) ( 118 ) Feb., 2008 Vol.20 No.1 2 ( ) cbls@sibs.ac.cn ( ) ( 782 ) by Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Edited by GUAN Xing-hua, YUE Dong-fang, YU Jian-rong Editorial Board of (319 Yueyang Road, Shanghai , China) Editor-in-Chief LIN Qi-shui Sponsored by Department of Life Sciences, National Natural Science Foundation of China; Bureau of Life Sciences and Biotechnology, Chinese Academy of Sciences; Division of Life Sciences and Medicine, Chinese Academy of Sciences; Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Published by Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Distributed by Shanghai Post Office Subscripted by Local Post Offices Overseas Distributed by China National Publishing Industry Trading Corporation (P.O.Box 782, Beijing, China) Cable: CNPITC ISSN CN /Q : : DK : 25.00

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