( GR, E. C )

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1 ( ), 2006, 28 ( 3) : CN / N ISSN Journal of Yunnan University Ξ 1, 2, 1, 3 (1., ;2., ; 3., ) : 3,,.,, GR,. : ; ;; GR :Q 945 :A : (2006) ( GR, E. C ), [1 ]., [2 ]., GR, GR [3 6 ]. GR,. ( ) [7 ], SOD,AsPOD,CA T, GR,. GR ( Zea m ays. L), 3., 0. 1 % HgCl 2 10 min, 5, 3 min, h, 6, 28 / 25 ( 12 h/ 12 h) 60 h,,, h 42 4 h, 28 4 h, ; 28, h ( 3) 10 h ( ), Ξ : : ( ) ; 211 ; (Z - 002). : ( ),,,.

2 3 : , lx 12 h, 8 d,. 8 d, [8 ],,. 6, 100, ( GR, EC ) GR Kngrzer [9 ] [10,11 ]. Bradford [12 ], GR Laemmli [13 ] Anderson [4 ]. GR GR 2, NADPH,NADP, GSSG, GSH Sigma.,,., GR 2,3, GR, GR. 3, GR,; 42 4 h, GR, 28 4 h, GR, GR, GR ( 4 h), 8 h,. GR,, GR, GR , % ; 312 %. 8 d, ( 1), Fig. 1 Effect of heat shock on survival percentage of two variety seedlings at 50 heat stress 1 8 d Tab. 1 Fresh weight,height and chlorophyll content of maize seedlings though 8 days recovery after different treatment ( ) / (mg g - 1 ) / g / cm

3 264 ( ) GR 3 ( 4) h 3 GR, 4 h 1 ; 4 h ; 5 h, GR ; 16 h, GR 2 2, 1 3. GR. ( 5), h 3 GR ; 4 h GR 1, 3 ; 4 h ; 5 h, 4,, 4 ; 10 h, 3 GR, 1,4., GR, GR. 2 3 GR Fig. 2 Changes of GR activity during heat shock,recovery and heat stressin maize seedling Qing No. 3 3 GR Fig. 3 Changes of GR activity during heat shock,recov2 ery and heat treatment in maize seedling Dahuang a :28 60 h GR ;b : 4 h GR ;c : 4 h GR ;d : 5 h GR ;e : 16 h GR 1 : ;2 : 4 3 GR Fig. 4 Electrophoresis pattern of GR isozymes duringheat shock, recovery andheat treatment in maize seedlings Qing No. 3 a :28 60 h GR ;b : 4 h GR ;c : 4 h GR ;d : 5 h GR ;e : 10 h GR 1 : ;2 : 5 GR Fig. 5 Electrophoresis pattern of GR isozymes duringheat shock, recovery andheat treatment in maize seedlings Dahuang

4 3 : ( ) [8,14 ]., [15 ]. [14,15 ]., [2 ]. GR, ( 6),,, [1 ]. 6 Fig. 6 The ascorbate - glutathione cycle in plants GR,,,., GR, Foyer GR [16 ], GR. GR, GR,. GR, GR, GR,,,.,GR. 1 GR Guy Carter,, GR [17 ]. [18 ]. GR,. : [1 ] MULL IN EAUX P M,CREISSEN G P. Glutathione re2 ductase : Regulation and role in oxidative stress [ M ]/ / Oxidative stress and the molecular biology of antioxidant defenses. United Kingdom : Cold Spring Harbor Labora2 tory Press,1997. [2 ] ALSCHER R G,DONAHU E J L,CRAMER C L. Re2 active oxygen species and antioxidants relationships in green cells[j ]. Physiol Plant,1997,100 : [3 ] FO YER C. Effects of elevated cytosolic glutathione re2 ductase activity on the cellular glutathione pool and pho2 tosynthesis in leaves under normal and stress conditions [J ]. Plant Physiol,1991,97 : [4 ] ANDERSON M D, PRASAD T K, STEWART C R. Change in isozyme profiles of catalas peroxidase and glu2 tathione reductase during acclimation to chilling in mesocotyls of maize seedlings[j ]. Plant Physiol, 1995, 109 : [5 ] EDWARDS. Synthesis and properties of glutathione re2 ductase in stressed peas[j ]. Planta,1994,192 : [6 ] ECHNOS. Salt stress2induced, responses in cucumber plants[j ]. J Plant Physiol,1997,150 : [7 ] PAR K S Y,SHIVAJ I R, KRANS J V,LU THE D S. Heat2shock response inheat tolerant and nontolerant variants of A grostis palust ris Huds[J ]. Plant Physiol, 1996,111 : [8 ] X H. [ M ].,,. :,1981.

5 266 ( ) 28 [9 ] KNȮ. RZER O C,DURN ER J,BȮ. GER P. Alterations in the antioxidative system of suspension2cultured soybean cells ( Glycine max) induced by oxidative stress [ J ]. Physiologia Plantarum,1996,97 : [10 ],,. ( ) [J ]. :,1999,21 (5) : [11 ],,. [J ]. :, 2003,25 (5) : [ 12 ] BRADFOR M M. A rapid and sensitive method for the quantification of microdram quantities of proteins uti2 lizing the principle of protein2dye2binding [ J ]. Analyt Biochem,1976,44 : [ 13 ] LACMML I U R. Clearage of structural proteins during the assembly of thehead of bacteriophage, T 4 [J ]. Na2 ture,1970,227 : [14 ] GON G M,VAN DER LU IT AH, KN IGHT M R,et al. Heat shock2induced changes of intracellular Ca 2 + level in tobacco seedlings in relation to thermotolerance [J ]. Plant Physiol,1998,116 : [15 ] SABEHA T A, WEISS D,LURIE S. Heat2shock pro2 teins and cross2tolerance in plants [ J ]. Physiol Plant, 1998,103 : [16 ] FO YER C. Photooxidative stress in plants [J ]. Plant physiol,1994,102 : [ 17 ] GU Y and CHARTER. Characterization of partially pu2 rified glutathione reductase from coldhardened and nonhardened spinach leaf tissue[j ]. Cryobiology,1984, 21 : [ 18 ] HAUSLADEN A. Purification and characterization of glutathione reductase isozymes specific for the state of coldhardiness of red spruce [J ]. Plant Physiol, 1994, 105 : Changes in activity of glutathione reductase and it s isozyme of maize seedlings during heat shock and heat stress GUO Li2Hong 1, CHEN Shan2na 2,WAN G De2bin 1, GON G Ming 3 (1. Department of Biology, Kunming Teachers College, Kunming ,China ; 2. School of Life Sciences, Yunnan University, Kunming ,China ; 3. Department of Life Sciences, Yunnan Normal University, Kunming ,China) Abtract : Two varieties of maize ( Zea m ays L.,cultivars Dahuang and Qing No. 3) were used in the study. The results showed that a heat shock ( HS) treatment significantly increased the survival percentage of two variety seedlings at heat st ress,confirming heat2shock increased heat tolerance of maize seedlings. More2 over,t he maize seedlings t hrough heat pret reat ment, retained relativelyhigher activity of glutat hine reductase ( GR, EC ) than the seedlings without heat2shock pretreatment. In the profiles glutathine reductase isozyme in the seedlings after heat2shock2pretreatment appeared a new band as compared with the seedlings wit hout heat2shock pret reat ment. Changes in isozyme profiles of GR had been different during heat st ress. These indicated t hat higher activity of GR and changes in isozyme profiles of GR were related to heat tolerance of maize seedlings. Key words : heat shock ; maize seedling ;glutat hine reductase ;glutat hine reductase isozyme