MALDI-TOF MS microbe taxonomy taxis = arrangement nimina = distribution. Cowan 3 classification. nomenclature.

Μέγεθος: px

Εμφάνιση ξεκινά από τη σελίδα:

Download "MALDI-TOF MS microbe taxonomy taxis = arrangement nimina = distribution. Cowan 3 classification. nomenclature."

Ημέρα Γιάνναρης
6 χρόνια πριν
Προβολές:

1 Kiyofumi OHKUSU microbe MALDI-TOF MS taxonomy taxis = arrangement nimina = distribution Cowan 3 classification nomenclature identification Department of Microbiology, Gifu University Graduate School of Medicine (1-1 Yanagido, Gifu)

2 8, International Code of Nomenclature of Bacteria ; ICNB strain species subspecies serovar phagovar pathovar biovar binomial nomenclature genus 2 specific epithet 1 DNA DNA DNA DNADNA DNA GC S rdna 16S rdna 1,500 bp S rrna 5

3 Mass Spectrometry Mass Spectrometry MS 3 MALDI-TOF MSMatrix Assisted Laser DesorptionIonization -Time of Flight Mass Spectrometer MALDI-TOF MS MALDI-TOF MS Matrix Assisted Laser DesorptionIonization Time of Flight Mass Spectrometer ; TOF MS 3 MALDI 337nm α-cyano-4- hydroxy cinnamic acidchca sinapinic acid - COOH H MALDI EI CI FAB ESI MALDI TOF

4 MALDI Laser DesorptionIonization K= mv 2 2 mv MALDI-TOF MS MS MALDI Biotyper mz

5 MALDI-TOF MS BD 3 1 AXIMA VITEK MS MALDI BiotyperVITEK MS MALDI-TOF MS MALDI-TOF MS MALDI-TOF MS 2,000 20,000 Da MALDI-TOF MS

6 MALDI BiotyperVITEK MS 1, 2 MALDI Biotyper Main SpectraMSP VITEK MS SuperSpectra TM

7 MALDI Biotyper MALDI-TOF MS 10 MALDI-TOF MS

8 MALDI Biotyper MALDI Biotyper 2,800 VITEK MS 2,980 MALDI-TOF MS Escherichia coli Shigella flexnerimycobacterium tuberculosis M. bovis Bacillus cereus B. anthracis Yersinia pestis Y. pseudotuberculosis DNADNA Staphylococcus 90 Streptococcuss S. pneumoniae S. mitis grroup MALDI-TOF MS 16S rdna MALDI-TOF MS MALDI-TOF MS MALDI Biotyper Mycobacterium

Candida Cryptococcus 8, 9 MALDI-TOF MS 10 12 70 80 Bruker MALDI Sepsityper MALDI-TOF MS MALDI Sepsityper TM 5 1 800 10 5 13, 14 MALDI-TOF

9 Candida Cryptococcus 8, 9 MALDI-TOF MS Bruker MALDI Sepsityper MALDI-TOF MS MALDI Sepsityper TM , 14 MALDI-TOF MS , 17-18, 19 MALDI-TOF MS 20 Family Genes Species Strain DNA-DNA 16S rrna GC mol PFGE Multilocus sequence typingmlst MALDI-TOF MS

10 1 Wayne LG et al.: Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol. : , Stackebrandt E, Ebers, J : Taxonomic parameters revisited : tarnished gold standards. Microbiology Today. : , Tanaka K, Ido Y, Akita S, et al.: Detection of High Mass Molecules by Laser Desorption Time-of-Flight Mass Spectrometry. SECOND JAPAN-CHINA JOINT SYMPO- SIUM ON MASS SPECTROMETRY ABSTRACT, Medical Technology. : , Senq P, Drancourt M, Gouriet F, et al.: Ongoing revolution in bacteriology : routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis. : , Bizzini A, Durussel C, Bille J, et al.: Performance of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Identification of Bacterial Strains Routinely Isolated in a Clinical Microbiology Laboratory. J Clin Microbiol. : , Sffert R, Cunningham S, Ihde S, et al.: Comparison of Bruker Biotyper Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometer to BD Phoenix Automated Microbiology System for Identification of Gram-Negative Bacilli. J Clin Microbiol. : , Marklein G, Josten M, Klanke U, et al.: Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Fast and Reliable Identification of Clinical Yeast Isolates. J Clin Microbiol. : , Dhiman N, Hall L, Wohlfiel S, et al.: Performance and Cost Analysis of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for Routine Identification of Yeast. J Clin Microbiol. : , Scola L, Raoult D : Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry. PLoS One. : e8041, Kok J, Thomas L, Olma T, et al.: Identification of bacteria in blood culture broths using matrix-assisted laser desorption-ionization Sepsityper TM and time of flight mass spectrometry. PLoS One. : e23285, Schmidt V, Jarosch A, Marz P, et al.: Positive blood culture by matrix-assisted laser desorption ionization time-offlight mass spectrometry. Eur J Clin Microbiol Infect Dis [Epub ahead of print]. 13Ferreira L, Sanchez-Juanes F, Gonzalez-Avila M, et al.: Direct Identification of Urinary Tract Pathogens from Urine Samples by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. J Clin Microbiol. : , Hyvang Hartmeyer G, Kvistholm Jensen A, Bocher S, et al.: Mass spectrometry : pneumococcal meningitis verified and Brucella species identified in less than half an hour. Scand Infect Dis. : , Proteomics. : , Warscheid B, Fenselau C : A targeted proteomics approach to the rapid identification of bacterial cell mixtures by matrix-assisted laser desorptionionization mass spectrometry. Proteomics. : , Bittar F, Ouchenane Z, Smati F, et al.: MALDI-TOF-MS for rapid detection of staphylococcal Panton-Valentine leukocidin. Int J Antimicro Agents. : , La Scola B, Campocasso A, et al.: Tentative characterization of new environmental giant viruses by MALDI-TOF mass spectrometry. Intervirology. : , Burckhardt I, Zimmermann : Using Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry To Detect Carbapenem Resistance within 1 to 2.5 Hours. J Clin Microbiol. : , Sparbier K, Schubert S, Weller U, et al.: Rapid detection of resistance against -lactam antibiotics. J Clin Microbiol [Epub ahead of print]. 20Welker M, Moore E.: Applications of whole-cell matrixassisted laser-desorptionionization time-of-flight mass spectrometry in systematic microbiology. Syst Appl Microbiol. : 2-11, 2011.

Σχετικά έγγραφα

c Key words: cultivation of blood, two-sets blood culture, detection rate of germ Vol. 18 No

c Key words: cultivation of blood, two-sets blood culture, detection rate of germ Vol. 18 No 2008 245 2 1) 1) 2) 3) 4) 1) 1) 1) 1) 1), 2) 1) 2) 3) / 4) 20 3 24 20 8 18 2001 2 2 2004 2 59.0 2002 1 2004 12 3 2 22.1 1 14.0 (CNS), Bacillus c 2 p 0.01 2 1 31.3 41.9 21.4 1 2 80 CNS 2 1 74.3 2 Key words: