BD OptEIA ELISA Sets ELISA

BD OptEIA ELISA Sets ELISA

BD OptEIA ELISA Sets ELISA CaptureDetection Detection 9620 170,000 ELISAEnzymed-Linked ImmunoSorbent Assay CaptureDetection BD OptEIA ELISA Set 2

BD OptEIA ELISA Set BD OptEIA ELISA Set2 1 1) Capture 2) Detection 3) 4) HRP 5) Instruction/Analysis Certificate 2 1) Capture 2) HRP Detection 3) 4) Instruction/Analysis Certificate Mouse IFN-γ BD OptEIA Mouse IFN-γ ELISA Set Cat. No. 555138 BD OptEIA Reagent Set BpH9.5 buffer Cat. No. 550534 BD Falcon 96 ELISA Cat. No. 353279 CaptureDetection 9620 Coating BufferWash Buffer Buffer 9620 ELISA OptEIA ELISA SetReagent SetPrice List67 96100 3

BD OptEIA ELISA Set Instruction/Analysis Certificate A Standard Preparation D Addition of Standard and Sample -80 B Coating 96Capture 1. CaptureCoating Buffer 2. Wash Buffer Assay DiluentStandard 5. Assay DiluentStandard 6. Capture StandardInstruction/Analysis Certificate duplicate2 7. 2. 300 µl 300 µl 300 µl 300 µl 300 µl 300 µl Stock Standard 500pg/mL 250pg/mL 125pg/mL 62.5pg/mL 31.3pg/mL 15.6pg/mL 7.8pg/mL C Blocking 3. Assay Diluent * 4. 2. * BD OptEIAAssay Diluent 4

E Detection G Stopping Reaction Instruction/Analysis CertificateWorking Detector 11. Stop Solution 1 DetectionAvidin-HRP 2 HRPDetection 8. Working Detector 9. H Caliculation of Results Detection Antibody Avidin-HRP Assay Diluent 12. 450nm 450nm570nm * Standard Curve * F Coloring Reaction TetramethylbenzidineTMBH2O2 10. Substrate Solution H2O2 TMB 1.5.BD OptEIA ELISA Set 5BD OptEIA Reagent Set A B9620 7 1. Coating Buffer 0.1M Sodium Carbonate, ph9.5 0.2M Sodium Phosphate, ph6.5 7 2. Assay Diluent10% FBSPBS 3. Wash Buffer0.05% Tween-20PBS 4. Substrate Solution TetramethylbenzidineTMB 5. Stop Solution1M H3PO42N H2SO4 5

Product List BD OptEIA ELISA Set CaptureDetectionStandard Human OptEIA ELISA Set 20 170,000 Humam Eotaxin 555175 6.3-400 pg/ml B Humam GM-CSF 555126 4.7-300 pg/ml B Humam IFN-γ 555142 4.7-300 pg/ml B Humam IL-1β 557953 3.9-250 pg/ml B Humam IL-2 555190 7.8-500 pg/ml B Humam IL-2 srα 559104 7.8-500 pg/ml B Humam IL-4 555194 7.8-500 pg/ml B Humam IL-5 555202 7.8-500 pg/ml B Humam IL-6 555220 4.7-300 pg/ml B Humam IL-8 555244 3.1-200 pg/ml B Humam IL-10 555157 7.8-500 pg/ml B Humam IL-12p40 555171 31.3-2000 pg/ml B Humam IL-12p70 555183 7.8-500 pg/ml B Humam IL-15 559268 7.8-500 pg/ml B Humam IP-10 550926 7.8-500 pg/ml B Humam LT-αTNF-β 550995 15.6-1000 pg/ml B Humam MCP-1 555179 7.8-500 pg/ml B Humam MIG 550998 31.3-2000 pg/ml B Humam TGF-β1 559119 62.5-4000 pg/ml B Humam TNF 555212 7.8-500 pg/ml B Humam TRAIL 550948 62.5-4000 pg/ml B Human OptEIA ELISA Set 5 78,000 Humam C5b-9 558315 B Humam Active Caspase-3 550930 B Mouse OptEIA ELISA Set 170,000 6 Mouse GM-CSF 555167 15.6-1000 pg/ml B Mouse IFN-γ 555138 31.3-2000 pg/ml B Mouse IFN-γAN-18 551866 3.1-200 pg/ml B Mouse IgE 555248 1.6-100 ng/ml B Mouse IgG2a 552576 3.1-200 ng/ml B Mouse IL-1α 550347 31.3-2000 pg/ml B Mouse IL-1β 559603 31.3-2000 pg/ml B Mouse IL-2 555148 3.1-200 pg/ml B Mouse IL-3 555228 7.8-500 pg/ml B Mouse IL-4 555232 7.8-500 pg/ml B Mouse IL-5 555236 15.6-1000 pg/ml B Mouse IL-6 555240 15.6-1000 pg/ml B Mouse IL-10 555252 31.3-2000 pg/ml A Mouse IL-12p40 555165 15.6-1000 pg/ml A Mouse IL-12p70 555256 62.5-4000 pg/ml A Mouse MCP-1 555260 15.6-1000 pg/ml A Mouse TNFMono/Mono 555268 15.6-1000 pg/ml A Mouse TNFMono/Mono 558534 15.6-1000 pg/ml B 20098

Product List ELISA Set Rat OptEIA ELISA Set 170,000 Rat IFN-γ 558861 31.3-2000 pg/ml B Rat IL-4 555198 1.6-100 pg/ml B Rat IL-6 550319 78-5000 pg/ml B Rat IL-10 555134 15.6-1000 pg/ml A Rat MCP-1 555130 31.3-2000 pg/ml B Rat TNF 558535 31.3-2000 ps/ml B ELISA Set Monkey OptEIA ELISA Set 170,000 Monkey IFN-γ 551492 B Monkey IL-2 551494 B ELISA Set BD OptEIA ELISA Set Description Cat.No. Size BD OptEIA ApH6.5 buffer 550536 1 set 60,000 BD OptEIA BpH9.5 buffer 550534 1 set 60,000 Contents1) Assay Diluent, 2) Substrate Reagent A, 3) Substrate Reagent B, 4) Coating Buffer, 5) Wash Concentrate (20X), 6) Stop Solution Set ACat.No.550536Set BCat.No.550534OptEIA ELISA Set Product List9620 Description Cat.No. Size Assay Diluent 555213 500 ml 30,000 Contentsanimal serum in a buffered solution with 0.15%ProClin-150 as preservative. Description Cat.No. Size TMB Substrate 555214 1 set 20,000 ContentsSubstrate reagent A - Hydrogen peroxide in a buffered solution Substrate reagent B - 3,3', 5,5' tetramethiylbenzidine in organic solvent + 2N H2SO4 or 1M H3PO4 ELISA Set BD OptEIA ELISA Set 34,000 Description Cat.No. Size BD Falcon 96ELISA 353279 100/ BD Falocn 96ELISA ELISA 4 3.5 3 2.5 2 1.5 1 0.5 0 8000 200 500 125 31 ng/ml Ig G Clear Enhanced 96-well (Cat. No. 353279) Clear TC treated 96-well (Cat. No. 353075) 20098 7

Product List OptEIA ELISA Kit 962CaptureDetection Human OptEIA TM ELISA Kit Description Cat.No Size C3a 550499 1 plate 7.3 pg/ml C4a 550947 1 plate 6.2 pg/ml C5a 557965 1 plate 0.047 ng/ml IFN-γ 550612 2 plates 1 pg/ml IL-1β 557966 2 plates 3.9 pg/ml IL-2 550611 2 plates 1 pg/ml IL-4 550614 2 plates 2 pg/ml 95,000 Description Cat.No Size IL-6 550799 2 plates 2.2 pg/ml IL-8 550999 2 plates 0.8 pg/ml IL-10 550613 2 plates 2 pg/ml IL-12p40 551116 2 plates 3.9 pg/ml IL-12p70 559258 2 plates 4 pg/ml MCP-1 559017 2 plates 1 pg/ml TNF 550610 2 plates 2 pg/ml Mouse OptEIA TM ELISA Kit Description Cat.No Size IFN-γ 558258 2 plates 0.762 pg/ml IL-6 550950 2 plates 3.8 pg/ml 95,000 Description Cat.No Size TNFMono/Poly 559732 2 plates 5 pg/ml Rat OptEIA TM ELISA Kit Description Cat.No Size TNF 550734 2 plates 13 pg/ml 95,000 BD OptEIA TM ELISA Kit Antibody Coated 96 breakable Wells12 strips8 wells Detection Antibody Standards Enzyme Concentrate Standard / Sample Diluent Enzyme DiluentCat.No.550499, 550947 ELISA Diluent Wash Concentrate TMB One-Step Substrate Reagent Substrate A & Substrate BCat.No.559017, 559258 Stop Solution Plate Sealers 20098 BDBDBecton, Dickinson and Company 2009 BD 66-006-05 R1-0706-003-048