2011 48 4 909 914 * 1 2** 2 2 2 2 2*** 1. 110161 2. 100081 026000 3 Calliptamus italicus L. 3 LC 50 LC 90 1. 34 14. 17 mg / L LC 50 LC 90 2. 09 45. 22 mg / L 50 mg / L 14 d 87% 100% 50 mg / L 50% The toxicity of three chitin synthesis inhibitors to Calliptamus italicus Othoptera Acridoidea ZHAO Zhong-Wei 1 2** ZHANG Yang 2 CAO Guang-Chun 2 GAO Song 2 Yasen Shali 2 ZHANG Ze-Hua 2*** 1. College of ShenYang Agricultural University Shenyang 110161 China 2. Key Laboratory for Biological Control of Ministry of Agriculture Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100081 China Xilinhot Key Field Station for Grassland Pest Control of Ministry of Agriculture Xilinhot 026000 China Abstract The effectiveness of three chitin synthesis inhibiting insecticides Diflubenzuron and were tested on the eggs and 3rd instar larvae of Calliptamus italicus L.. The results show that had the highest control efficacy with LC 50 and LC 90 of 1. 34 mg / L and 14. 17 mg / L respectively. Diflubenzuron was the next most toxic with LC 50 and LC 90 of of 2. 09 mg / L and 45. 22 mg / L respectively and the least toxic with a rate of population decrease < 50% at a concentration of 50 mg / L. and Diflubenzuron also decreased the larval molting and egg hatching rate of C. italicus. Key words Calliptamus italicus chitin synthesis inhibitor lethal concentration molting rate Calliptamus italicus L. hm 2 2001 Orthoptera Acridoidea Acridoidea Calliptamus Serville 2006 17 45 2006 2000 2010 Larami 1967 1. 33 hm 2 40 ~ 50 / m 2 2000 100 ~ 130 / m 2 6 667 hm 2 685. 7 * 20090321 201003079 nycytx-2009-37 **E-mail zhaozhong1985@ 163. com *** E-mail lgbcc@ 263. net 2011-4-20 2011-5-16
910 48 insect growth regulator IGR Fresco 100 10 1 0. 1 0. 01 mg / 1995 L 20 3 10 s 2 h Licht 2004 2006 20% 1993 Garside 1997 1 cm 30 L D = 14 10 2010 1998 1. 2. 2 3 3 3 1999 Post et al. 1974 80 2 h 1 1 1 1999 2 0. 08 mg / L 0. 08 mg / L 3. 125 mg / L 3 3 0. 4 mg / L 0. 4 mg / L 6. 25 mg / L 4 2 mg / L 2 mg / L 12. 5 mg / L 1 25 mg / L 1. 1 6 50 mg / L 50 mg / L 1. 1. 1 2010 8 50 mg / L 3 15 4 48 30 h 80 2 h 20% 33 ± 1 RH 45% 3 6% 21 cm 16. 5 cm 8 00 20 00 14 d 4. 6 cm 1. 1. 2 95. 09% 95% > 99% 100 mg / L 300 mg / L 300 mg / L 5 10 mg / L 10 mg / L 1. 2. 3 2010 1. 2 % = 1. 2. 1 3 100 % =
4 911-100 DPSV7. 05 2010 Duncan 1 2 0. 01 0. 1 1 mg /L 3 2. 1 3 2 10 mg / L 100 mg / L 1 mg / ml 1 10 mg / L 3% 5% 100 mg / L Table 1 1 3 The egg hatching rate of Calliptamus italicus effected by and mg / L Concentration Acetone 0. 01 0. 63 ± 0. 10a 0. 58 ± 0. 12a 0. 52 ± 0. 2a 0. 65 ± 0. 18a 0. 1 0. 50 ± 0. 13a 0. 55 ± 0. 28a 0. 53 ± 0. 2a 0. 73 ± 0. 13a 1 0. 57 ± 0. 13a 0. 57 ± 0. 08a 0. 42 ± 0. 08a 0. 52 ± 0. 26a 10 0. 68 ± 0. 28a 0. 50 ± 0. 26a 0. 05 ± 0. 05b 0. 03 ± 0. 06b 100 0. 52 ± 0. 15a 0. 77 ± 0. 32a 0. 00 ± 0. 00b 0. 0017 ± 0. 003b ± 0. 05 Data in the table are mean ± SD and followed by different letters in the same column indicate significant difference at 0. 05 level. The same below. 2. 2 3 mg / L 12 ~ 14 100% 2 3 3 1. 34 mg / L 2. 09 mg / L LC 90 14. 17 mg / L 45. 22 mg / L 3 50% 4 3 2 6 d 3 10 d LD 50 LD 90 50 mg / L LD 50 LD 90 43% 12. 5 mg / L 6 5. 6 3. 32 47% 10 mg / L 10 97% 50 LD 50 LD 90
912 48 Fig. 1 1 Egg hatching Calliptamus italicus A CK B C Fig. 2 2 Dead performance of Calliptamus italicus effected by and A CK B C Different treatments Table 2 2 3 3 The population decline rate of 3rd instar larvae of Calliptamus italicus effected by and 6 d 10 d 14 d 6 d 10 d 14 d 6 d 10 d 14 d 1 0. 07 ± 0. 06b 0. 06 ± 0. 03a 0. 17 ± 0. 06b 0. 07 ± 0. 06c 0. 17 ± 0. 06d 0. 17 ± 0. 06bc 0. 067 ± 0. 06c 0. 17 ± 0. 06d 0. 17 ± 0. 06d 2 0. 07 ± 0. 06b 0. 10 ± 0. 06a 0. 27 ± 0. 15ab 0. 27 ± 0. 06c 0. 50 ± 0. 17bc 0. 70 ± 0. 26b 0. 20 ± 0. 10bc 0. 20 ± 0. 10cd 0. 33 ± 0. 06bc 3 0. 27 ± 0. 12ab 0. 23 ± 0. 13a 0. 43 ± 0. 15ab 0. 17 ± 0. 06bc 0. 40 ± 0. 10c 0. 40 ± 0. 10a 0. 20 ± 0. 10bc 0. 40 ± 0. 17bc 0. 40 ± 0. 17c 4 0. 30 ± 0. 20ab 0. 15 ± 0. 09a 0. 47 ± 0. 06ab 0. 47 ± 0. 21ab 0. 70 ± 0. 10ab 0. 77 ± 0. 15a 0. 37 ± 0. 12ab 0. 53 ± 0. 21b 0. 60 ± 0. 20ab 5 0. 30 ± 0. 20ab 0. 30 ± 0. 17a 0. 50 ± 0. 26a 0. 57 ± 0. 12a 0. 77 ± 0. 06a 0. 77 ± 0. 06a 0. 50 ± 0. 10a 0. 97 ± 0. 06a 0. 97 ± 0. 06a 6 0. 40 ± 0. 17a 0. 15 ± 0. 09a 0. 43 ± 0. 15ab 0. 53 ± 0. 12a 0. 83 ± 0. 21a 0. 87 ± 0. 23a 0. 50 ± 0. 10a 0. 87 ± 0. 06a 1. 00 ± 0a 2. 3 3 0. 08 0. 4 mg / L 2 mg / L 5 10 mg / L 50 mg / L
4 913 Table 3 Pesticides 3 3 3 The lethal concentration of 3 chitin synthesis inhibitors on 3rd larvae of Calliptamus italicus LC 10 mg / L LC 50 mg / L LC 90 mg / L Virulence regression Related coefficient y = 4. 84073 + 1. 25124x 0. 9179 0. 127 ± 0. 033 1. 34 ± 0. 62 14. 17 ± 5. 54 y = 4. 3073 + 0. 2611x 0. 66216 0. 0056 ± 0. 135 450. 13 ± 12. 65 y = 2. 90616 + 2. 03906x 0. 9477 0. 81 ± 0. 2451 2. 09 ± 0. 76 45. 22 ± 17. 85 Table 4 4 3 3 The lethal dose of 3 chitin synthesis inhibitors on 3rd larvare of Calliptamus italicus Pesticides Virulence regression Related coefficient LD 10 μg LD 50 μg LD 90 μg y = 5. 024 + 1. 1613x 0. 9744 0. 075 ± 0. 0123 0. 95 ± 0. 37 12. 1 ± 4. 34 y = 4. 434 + 0. 1839x 0. 6687 0. 00013 ± 0. 13 y = 3. 942 + 1. 4577x 0. 9163 0. 70 ± 0. 091 5. 32 ± 2. 1 40. 27 ± 7. 16 Table 5 5 3 The molting rate of Calliptamus italicus effected by 3 chitin synthesis inhibitors Pesticides 1 13. 71 ± 0. 01a 13. 71 ± 0. 01a 13. 71 ± 0. 01a 2 12. 28 ± 0. 01a 12. 49 ± 0. 04a 12. 51 ± 0. 03a 3 13. 87 ± 0. 02a 14. 9 ± 0. 04a 11. 31 ± 0. 02a 4 12. 69 ± 0. 02a 13. 2 ± 0. 05a 9. 21 ± 0. 02ab 5 12. 43 ± 0. 06a 1. 19 ± 0. 02b 8. 42 ± 0. 01ab 6 13. 15 ± 0. 02a 0. 71 ± 0. 01b 5. 69 ± 0. 04b 50 mg / L 10 mg / L 5% 3 3 1955 3 20% 3 3 12. 5 mg / L 3 2 cm 4 5 6 47% 10 mg / L
914 48 10 97% 2001.. 50 mg / L 6 46 47. Larami EG 1967. Locusts of Kazakhstan Central Asia and 100% Adjacent Territories. Larami Association for Applied Akridology International University of Wyoming. 387. 2006... 10 mg / L 50 mg / L 576 578. 13. 701% 10 Licht O Jungmann D Ludwichowski K Nagel R 2004. Long-term effects of fenoxycarb on two mayfly species in artificial indoorstreams. Ecotoxicol. Environ. Saf. 58 50 mg / L 2 246 255. 1998.. 3 29 31. Post LC De Jong BJ Vincent WR 1974. 1-2 6- References 2006.. 83. 27 2 88 91. 2010. DPS 2000.... 315 398. 5 341 345. Garside CS Hayes TK Tobe SS 1997. Degradation of Dipallatostains by hemolymph from the cockroach Diplpptera punctata. Peptides 18 1 17 25. 2010. 6 256 258.. 17 2010. 3 536 542.. 3 67 69. 1993.. 19 4 31 33. disubstituted benzoyl -3-phenylurea insecticides inhibitors of chitin synthesis. Pestic. Biochem. Physiol. 4 4 73 1999... 36 41. 1955..