Salvia macilenta Boiss.

0 4 66664 (9) * 2 * : akhgar@iauk.ac.ir 2 : 9 : 9 : 9 Salvia 8 7.. 9. (GC) (GC/MS). 46 %99/7. (%6/4) (%7/6) (%/4) (%/). 26 %00. (%4/) (%0/) (%8/8) (%6/8). 8 %97/. (%2/) (%6/8) (%8/6) (%8/). 0 %88/2 (%8/) (%6/4) (%/8) (%0/6). S. macilenta. : ) (.. (Lamiaceae) ).(76. 87 000 49 ).(7 Salvia 900..(Walker & Sytsma, 2007) 8 7. ).(8 DOI: http://dx.doi.org/0.22092/ijmapr.204.9847

0 4 67.(Salimpour et al., 20).(Alizadeh & Shaabani, 202) Kunduhoglu et al., 20) Kamatou et al., 2008 (Yousefzadi et al., 2007 ) Loizzo et (al., 2008 (Kelen & Tepe, 2008) (Kamatou et al., 200) Eidi ) & Eidi, 2009 (Sairafianpour et al., 200 (Loizzo et al., 2007).. Salvia macilenta Boiss. ) (. 0. 20. 2 6. 4. 6 /. ) ( ) Rechinger,.(982 S. macilenta.(sonboli et al., 200).. Salvia macilenta 9 ) 700 800 (. (Rechinger, 982) 808.. 00.. GC Shimadzu A DB 0 0/2 0/2. 60 220 220. 20 FID ) ( 270. GC/MS Agilent 97C Agilent 7890A HPMS 0 0/2 0/2. 60 240 240.

68 20 270. 70 40 40. (GC). (GC/MS). (RI) (C 7 C 2 ) GC. Adams, 2004) (Davies, 990 WILEY NIST GC/MS.. S. macilenta %/6 %/ %0/6 % (w/w).. 46 %99/7. (%6/4) (%7/6) (%/4) (%/). 26. (%4/) (%0/) (%8/8) (%6/8). 8 %97/. (%2/) (%6/8) (%8/6) (%8/). 0 %88/2. (%8/) (%6/4) (%/8) (%0/6). Salvia macilenta tricyclene 92 2 αthujene 927 0/7 αpinene 97 6/4 4/ 2/ 4 camphene 948 /8 8/8 /0 sabinene 970 6 βpinene 97 7/6 0/ 2/ 7 myrcene 988 / 2/2 8 octanol 99 9 αphellandrene 00 0/

0 4 69 0 terpineneα 04 /0 /6 pcymene 02 /6 / 2 limonene 027 4/ 6/8 2/4,8cineole 00 /7 2/7 /8 4 (Z)βocimene 08 0/6 /2 (E)βocimene 048 / /2 6 γterpinene 08 2/ /0 7 terpinolene 086 0/7 0/8 8 linalool 098 0/7 9 αcampholenal 24 20 transpinocarveol 6 2 camphor 42 0/6 22 borneol 66 /4 / 8/ / 2 nnonanol 67 /7 24 terpinen4ol 7 /4 /2 / 2 αterpineol 88 0/8 26 myrtenol 94 0/2 27 neral 240 0/2 28 geranial 268 29 ndecanol 270 6/2 0 bornyl acetate 287 2/6 2/ 6/8 /8 αylangene 7 2 αcopaene 74 /6 /7 βcaryophyllene 49 / / /2 4 aromadendrene 48 αhumulene 4 0/ 6 alloaromadendrene 460 7 γmuurolene 476 /4 8 βselinene 487 0/6 9 αselinene 496 / 40 αmuurolene 498 4 γcadinene 0/6 42 myristicin 8 /6 4 δcadinene 2 2/4 2/ 44 αcalacorene 4

660 4 Elemol 47 2/6 46 Caryophyllene oxide 8 / /7 / 47 viridoflorol 9 2/8 /4 8/ 48 Humulene epoxide II 608 /2 49 0epiγeudesmol 622 0 epicubenol 628 0/2 epiαcadinol 64 2 βeudesmol 6 /2 8/6 6/4 αeudesmol 6 /6 4 apiol 679 2/2 dibutyl phthalate 846 0/6 6 transferruginol 20 /8 62/0 86/2 2/6 4/2 /0 6/0 8/9 /0 2/ 9/0 8/ 9/9 4/0 /8 20/ 99/7 00 97/ 88/2 S. macilenta... (%62) (%4/2) (%/0) (%8/). (%86/2) (%/0) (%2/) (%). (%2/6) (%6/0) (%9/0) (%9/9). (%8/9) (%4/0) (%/8) (%20/)... S. officinalis

66 4 0 S. (Shawl et al., 200) %26/9 S. moorcraftiana (Javidnia et al., 200) %26/ macrosiphon.. %46/6 S. virgata (Sefidkon & Mirza, 999) Sefidkon & Khajavi, ) %24/7 S. verticillata Rustaiyan et al., ) %27/ S. aethiopis (999 Mirza & Ahmadi, ) %6/ S. atropatana (999 Rustaiyan et al., ) %6/4 S. palaestina (2000 D. (200. Salehi et al., ) D %44 S. xanthocheila Mirza & Baher ) %4/4 S. grossheimii (200 Kunduhoglu ) %6/6 S. verticillata (Nik, 200. (et al., 20 (%8/) (Ahmadi & Mirza, 999) S. malticaulis S. persepolitana (%27) (%7/) (Habibi et al., 2008) S. rhytidea (Sajjadi & Shahpiri, 2004) S. limbata (%2/) S. mirzayanii (%4/6 %) S. urumiensis (Javidnia et al., 2002) (%22/4) (Khaliazadeh et al., 20) (Kunduhoglu et al., 20) S. dicroantha. S. macilenta 8..( ).76.. 970.( ).7.. 768.(Velickovic et al., 200) S. macilenta. (%77).(Sonboli et al., 200). (%/2) (%6/).. Salvia S. macilenta (Rustaiyan et al., 999) %26 S. multicaulis Sefidkon & Khajavi, ) %9/4 S. santolinifolia (Habibi et al., 2004) %24/ S. eremophila (999 (Rustaiyan et al., 200) %2/7 S. limbata (Kunduhoglu et al., 20) %6/2 S. wiedemannii.. %4/4 S. candidissima (Bayrak & Akgul, 987) %9/2 S. tomentosa (Rustaiyan et al., 2000) %2/7 S. lereifolia (Yousefzadi et al., 2008) %0/6 S. chloroleuca. (Tucker et al., 996) %/7 S. clevelandii (Chalchat et al., 998) %0/8 S. officinalis %0/8 S. aytachii %0. (Baser et al., 997) Bayrak & Akgul, ) S. fruticosa (Demirci et al., 2002) S. aramiensis (987. 8

662 Kamatou, G.P.P., Makunga, N.P., Ramogola, W.P.N. and Viljoen, A.M., 2008. South African Salvia species: a review of biological activities and phytochemistry. Journal of Ethnopharmacology, 9(): 664672. Kamatou, G.P.P., Viljoen, A.M., GonoBwalya, A.B., van Zyl, R.L., van Vuuren, S.F., Lourens, A.C.U., Baser, K.H.C., Demirci, B., Lindsey, K.L., van Staden, J. and Steenkamp, P., 200. The in vitro pharmacological activities and a chemical investigation of three South African Salvia species. Journal of Ethnopharmacology, 02(): 8290. Kelen, M. and Tepe, B., 2008. Chemical composition, antioxidant and antimicrobial properties of the essential oils of three Salvia species from Turkish flora. Bioresource Technology, 99(0): 4096404. Khaliazadeh, M.A., Esmaeili, A., Rustaiyan, A., Eslami, B. and Masoudi, S., 20. Chemical composition of essential oils of three Salvia species growing wild in Iran. Chemistry of Natural Compounds, 46(6): 98987. Kunduhoglu, B., Kurkcuoglu, M., Duru, M.E. and Baser, K.H.C., 20. Antimicrobial and anticholinesterase activities of the essential oils isolated from Salvia dicroantha Stapf., Salvia Verticillata L. subsp. amasiaca (Freyn and Bornm.) and Salvia wiedemannii Boiss. Journal of Medicinal Plants Research, (29): 64846490. Loizzo, M.R., Saab, A.M., Tundis, R., Statti, G.A., Menichini, F., Lampronti, I., Gambari, R., Cinatl, J. and Doerr, H.W., 2008. Phytochemical analysis and in vitro antiviral activities of the essential oils of seven Lebanon species. Chemistry and Biodiversity, (): 46470. Loizzo, M.R., Tundis, R., Menichini, F., Saab, A.M., Statti, G.A. and Menichini, F., 2007. Cytotoxic activity of essential oils from Labiatae and Lauraceae families against in vitro human tumor models. Anticancer Research, 27(A): 29299. Mirza, M. and Ahmadi, L., 2000. Composition of the essential oil of Salvia atropatana Bunge. Journal of Essential Oil Research, 2(): 776. Mirza, M. and Baher Nik, Z., 200. Chemical composition of the essential oil of Salvia grossheimii from Iran. Chemistry of Natural Compounds, 46(): 82282. Rechinger, K.H., 982. Labiatae, Flora Iranica, No. 0, Akademische Druck and Verlagsanstalt, Graz, Austria, 90p. Rustaiyan, A., Akhgar, M.R., Masoudi, S. and Nematollahi, F., 200. Chemical composition of essential oils of three Salvia species growing wild in Iran: Salvia rhytidea Benth., S. limbata C. A. Mey. and S. palaestina Benth. Journal of Essential Oil Research, 7(): 2224. Rustaiyan, A., Masoudi, S., Monfared, A. and..8.. 740 Adams, R.P., 2004. Identification of Essential Oil Components by Gas Chromatography/ Quadrupole Mass Spectroscopy. Allured Publishing Corporation, Illinois, USA, 46p. Ahmadi, L. and Mirza, M., 999. Essential oil of Salvia multicaulis Vahl from Iran. Journal of Essential Oil Research, (): 289290. Alizadeh, A. and Shaabani, M., 202. Essential oil composition, Phenolic content, antioxidant and antimicrobial activity in Salvia officinalis L. cultivated in Iran. Advances in Environmental Biology, 6(): 22226. Baser, K.H.C., Duman, H., Vural, M., Adiguzel, N. and Aytac, Z., 997. Essential oil of Salvia aytachii M. Vural et N. Adiguzel. Journal of Essential Oil Research, 9(4): 489490. Bayrak, A. and Akgul, A., 987. Composition of essential oils from Turkish Salvia species. Phytochemistry, 26(): 846847. Chalchat, J.C., Michet, A. and Pasquier, B., 998. Study of clones of Salvia officinalis L. yields and chemical composition of essential oil. Flavour and Fragrance Journal, (): 6870. Davies, N.W., 990. Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicon and Carbowax 20M phases. Journal of Chromatography A, 0: 24. Demirci, B., Baser, K.H.C. and Tumen, G., 2002. Composition of the essential oil of Salvia aramiensis Rech. Fil. growing in Turkey. Flavour and Fragrance Journal, 7(): 22. Eidi, A. and Eidi, M., 2009. Antidiabetic effects of sage (Salvia officinalis L.) leaves in normal and streptozotocininduced diabetic rats. Diabetes and Metabolic Syndrome: Clinical Research and Reviews, (): 4044. Habibi, Z., Biniaz, T., Masoudi, S. and Rustaiyan, A., 2004. Composition of the essential oil of Salvia eremophila Boiss. native to Iran. Journal of Essential Oil Research, 6(): 727. Habibi, Z., Yousefi, M., Aghaie, H.R., Salehi, P., Masoudi, S. and Rustaiyan, A., 2008. Chemical composition of essential oils of Salvia persepolitana Boiss. and salvia rhytidea Benth. from Iran. Journal of Essential Oil Research, 20():. Javidnia, K., Miri, R. and Jamalian, A., 200. Composition of the essential oil of salvia macrosiphon Boiss. from Iran. Flavour and Fragrance Journal, 20(): 424. Javidnia, K., Miri, R., Kamalinejad, M. and Nasiri, A., 2002. Composition of the essential oil of Salvia mirzayanii Rech. f. & Esfand. from Iran. Flavour and Fragrance Journal, 7(6): 46467.

66 4 0 4(): 446. Shawl, A.S., Raina, V.K., Srivastava, S.K. and Kumar, T., 200. Essential oil composition of Salvia moorcraftiana. Journal of Essential Oil Research, (4): 2829. Sonboli, A., Fakhari, A.R. and Sefidkon, F., 200. Chemical composition of the essential oil of Salvia macilenta from Iran. Chemistry of Natural Compounds, 4(2): 6870. Tucker, A.O., Maciarello, M.J. and Clebsch, B.B., 996. Volatile leaf oil of Salvia clevelandii (Gray) Greene Gilman. Journal of Essential Oil Research, 8(6): 669670. Velickovic, D.T., Randjelovic, N.V., Ristic, M.S., Velickovic, A.S. and Smelcerovic, A.A., 200. Chemical constituents and antibacterial activity of the ethanol extracts obtained from the flower, leaf and stem of Salvia officinalis L. Journal of the Serbian Chemical Society, 68(): 724. Walker, J.B. and Sytsma, K.J., 2007. Staminal evolution in the genus Salvia (Lamiaceae): molecular phylogenetic evidence for multiple origins of the lever. Annals of Botany, 00(2): 79. Yousefzadi, M., Sonboli, A., Ebrahimi, S.N. and Hashemi, S.H., 2008. Antimicrobial activity of essential oil and major constituents of Salvia chloroleuca. Zeitschrift Fur Naturforschung C, 6( 6): 740. Yousefzadi, M., Sonboli, A., Karimi, F., Ebrahimi, S.N., Asghari, B. and Zeinali, A., 2007. Antimicrobial activity of some Salvia species essential oils from Iran. Zeitschrift Fur Naturforschung C, 62(78): 48. Komeilizadeh, H., 999. Volatile constituents of three Salvia species grown wild in Iran. Flavour and Fragrance Journal, 4(): 276278. Rustaiyan, A., Masoudi, S., Yari, M., Rabbani, M., Motiefar, H.R. and Larijani, K., 2000. Essential oil of Salvia lereifolia Benth. Journal of Essential Oil Research, 2(): 60602. Sairafianpour, M., Bahreininejad, B., Witt, M., Ziegler, H.L., Jaroszewski, J.W. and Staerk, D., 200. Terpenoids of Salvia hydrangea: two new, rearranged 20norabietanes and the effect of oleanolic acid on erythrocyte membrane. Planta Medica, 69(9): 84680. Sajjadi, S.E. and Shahpiri, Z., 2004. Chemical composition of the essential oil of Salvia limbata C. A. Mey. DARU, 2(): 9497. Salehi, P., Tolami, L.B. and Sefidkon, F., 200. Essential oil composition of Salvia xanthocheila Boiss. ex Benth. from Iran. Journal of Essential Oil Research, 7(4): 44244. Salimpour, F., Mazooji, A. and Akhoondi Darzikolaei, S., 20. Chemotaxonomy of six Salvia species using essential oil composition markers. Journal of Medicinal Plants Research, (9): 7980. Sefidkon, F. and Khajavi, M.S., 999. Chemical composition of the essential oils of two Salvia species from Iran: Salvia verticillata L. and Salvia santholinifolia Boiss. Flavour and Fragrance Journal, 4(2): 7778. Sefidkon, F. and Mirza, M., 999. Chemical composition of the essential oils of two Salvia species from Iran: Salvia virgata Jacq. and Salvia syriaca L. Flavour and Fragrance Journal,

Iranian Journal of Medicinal and Aromatic Plants, Vol. 0, No. 4, 204 664 Chemical composition of the essential oils from leaves, flowers, stems and roots of M.R. Akhgar *, P. Rajaei 2 and S. Amandadi * Corresponding author, Department of Chemistry, Faculty of Science, Kerman Branch, Islamic Azad University, Kerman, Iran Email: akhgar@iauk.ac.ir 2 Department of Microbiology, Faculty of Science, Kerman Branch, Islamic Azad University, Kerman, Iran MSc. Student, Department of Chemistry, Faculty of Science, Kerman Branch, Islamic Azad University, Kerman, Iran Received: September 202 Revised: November 202 Accepted: November 202 Abstract The genus Salvia, belonging to the Lamiaceae family, contains 8 species in Iran, 7 of which are endemic. In this study, was collected from BamJiroft road, Jebalbarez area, Kerman Province, Iran, in May 202. The essential oils of leaves, flowers, stems and roots of the plant were separately extracted using hydrodistillation method and analyzed by GC and GC/MS. In the leaf oil, 46 components were identified, representing 99.7% of the total oil, with αpinene (6.4%), βpinene (7.6%), borneol (.4%) and βcaryophyllene (.%) as the main constituents. The flower oil was characterized by higher amount of αpinene (4.%), βpinene (0.%), camphene (8.8%) and limonene (6.8%) among the 26 components comprising 00% of the total oil detected. Furthermore, 8 compounds were identified in the stem oil, representing 97.% of the total oil. αpinene (2.%), bornyl acetate (6.8%), βeudesmol (8.6%) and borneol (8.%) were found to be the major constituents. In the root oil, 0 components were identified, representing 88.2% of the total oil, with transviridiflorol (8.%), βeudesmol (6.4%), transferruginol (.8%) and dibutyl phthalate (0.6%) as the main constituents. Consequently, the leaf, flower and stem essential oils of S. macilenta were rich in monoterpenes, while αpinene, the dominant component of these oils, was not identified in the root oil. Key words:, Lamiaceae, essential oil, αpinene.