: (Thymus vulgaris L.) GC/MS

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1 (DPPH) * : * (021) : (021) : : mbb@modares.ac.ir : 85/9/10 : 84/8/6 : : (Thymus vulgaris L.) GC/MS. :. (Syzygium aromaticum L.) (Satureja hortensis L.) :. (DPPH ) / ( ) :... DPPH :. : 1 54

2 ....[11 20]. 1.[20] 1.[11 20] [11 20].[18 19] [21] (DPPH ) DPPH ) 515 ( M -1 cm DPPH DPPH DPPH.[21] DPPH.[21 22].[23] DPPH.[21] DPPH DPPH.[21] 5 :.[24 25] Myrtus cummunis L. 2 2,2-diphenyl-1-picrylhydrazyl radical..[1 2 3]. -..[4 5]. TBHQ BHA BHT.[6 7 8].[9 10].[11] -.[ ]

3 Carvacrol Thymol Eugenol GC/MS. DPPH. ( ) GC/MS HP 6890N 0/25 30 split split. HP-5MS 0/25 :. 20:1 :ratio / cm/s HP 5973N. DPPH + AH DPPH LH + A DPPH + R DPPH R DPPH ( ) DPPH ( ).[21] ( ) IC50 50.[21] DPPH.. ١ ( ) 3 2 ( ) 1 Thymus vulgaris L. 3 Syzygium aromaticum L. 2 Satureja hortensis L. 56

4 ( ) 5 ( ). ( /5 0) ( 19/59) ( 33/14) ( 6/52) ( 16/00) 21/59) ( 22/16) 10/30) ( 12/21) ( ( 72/37) ( ( 13/14) ( 15/94).. DPPH EI 69/ ) Memert /5 DPPH (. (mg/ml) 1/4 DPPH 0/2 0/ /6 1/4. DPPH ١ DPPH. =A Sample (A %RSA = = %RSA. = A Control.[3] Control A A Control Sample ) 100 IC50. IC DPPH 1 Radical scavenging activity (RSA)

5 -1 ( ) 0/12 10/59 α-phellandrene 1/84 0/34 α-pinene 0/34 0/53 12/93 13/14 β -Pinene β-octanone 4 0/72 14/27 α-terpinene 6/52 15/23 ρ-cymene 0/44 15/35 Limonene 0/67 15/50 1,8-Cineol 2/43 16/77 γ-terpinene 16/00 19/07 Linalool 0/51 19/14 Ho-trienol 0/55 22/68 3-Cyclohexene 0/42 23/32 α-terpineol 0/81 23/57 Estragol 0/88 25/12 Methyl thymylether 19/59 25/67 Carvacrol 0/35 27/72 Trans-anethol 33/14 28/36 Thymol 0/50 30/90 Thymyl acetate 0/11 31/13 Neryl acetate 0/81 31/94 Granyl acetate 2/03 33/94 α-caryophyllene 0/17 35/41 β-humelen 1/52 40/83 Caryophyllene oxide 0/25 42/84 Vulgarol 0/15 43/58 Aromandrene 58

6 /24 1/77 0/15 0/92 1/98 0/12 2/93 10/30 1/55 12/21 0/17 21/59 22/16 0/75 0/34 ( ) 10/61 10/99 11/66 12/96 13/16 14/44 14/79 15/36 15/94 17/02 22/8 28/87 29/18 31/88 34/86 α-thujene α-pinene Camphene β-pinene Myrecene α-phellandrene α-terpinene ρ-cymene Limonene γ-terpinene Terpinene-4-ol Carvacrol Thymol Carvacryl acetate Aromanderene -3 0/10 0/34 72/37 15/94 2/62 0/44 13/14 0/18 1/06 ( ) 26/81 30/58 31/51 43/29 35/29 37/18 38/26 40/63 40/87 Chavicol Cubenere Eugenol β-caryophyllene α-humelene E,E-Frances Eugeyl acetate +Spathulenol Caryophyllene oxide

7 IC50 (mg) c T0 T120 T180 :T0.( 3) (IC50) :T :T A120 A180 IC50 (mg) ( ) A A IC50 (mg) 7 S S ( ) S S

8 C120 C180 IC50 (mg) ( ) C C ( ) /5 120 ( ) /

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