* TGF-β1 215600 diabetic nephropathies DN 24 urine protein quantitation in 24 hours 24hUTP serum creatinine Scr -β1 transforming growth factor-β1 TGFβ1 STZ DN SD 10 10 10 8 24hUTP Scr HE ELISA TGF-β1 24hUTP Scr TGF-β1 P < 0. 05 24hUTP Scr TGF-β1 P < 0. 05 P < 0. 8 DN DN DN 24hUTP TGF-β1. TGF-β1 J. 2018 33 11 2094-2098. TGF-β1 24 -β1 DOI 10. 16368 /j. issn. 1674-8999. 2018. 11. 496 R285. 5 A 1674-8999 2018 11-2094 - 05 Effect of Yiqi Yangyin Tongluo Prescription on TGF-β1 Expression in Model Rats with Diabetic Nephropathy DU Zhenfang QIANG Sheng HUANG Min MA Xiaowei Chinese Medicine Hospital of Zhangjiagang City Zhangjiagang Jiangsu China 215600 * SYSD2013156 7. 2 A Once-Daily Incretin Mimetic Injection for Type-2 Diabetes J. P CRP IL-6 TNF-α J. T 2017 42 11 676-711. 2017 12 7 1009-1012. 8. 2 60 J. 2014 29 3 30-34. Meta J. 2017 37 7 757-761. 9. 2 2014 6 19 114-115. J. 2017 23 8 15. 2 220-225. J. 2016 27 22 3151-10 SUWITA B M FRISKA D WIDJAJA D S. Multidiscipline Care for Type 2 Diabetes Patients from General to Asian Population J. Acta Med Indones 2017 49 3 259-266. 11. 2 hs- 1977 - CRP IL-6 J. 2014 10 209-212. 12 LEON N LACOURSIERE R YAROSH D. Lixisenatide Adlyxin 2094 13. 2 14. 2 J. 3153. 2018-07 - 16
Abstract Objective To analyze the effect of Yiqi Yangyin Tongluo Prescription on 24 hour urine protein quantitation 24hUTP serum creatinine Scr level and transforming growth factor-β1 TGF-β1 expression in diabetic nephropathy DN rats. Methods The DN model was prepared by high-sugar and high-fat diet combined with STZ intraperitoneal injection. The model SD rats were randomly divided into 10 model groups 10 high-dose groups 10 medium-dose group 10 low-dose group and 10 normal groups. After 8 weeks of drug treatment 24hUTP and Scr were detected in each group. The pathological changes of residual kidney and the expression of TGF-β1 in kidney tissue were detected by HE staining and ELISA. Results Compared with the normal group 24hUTP Scr and TGF - β1 were increased in the other groups P < 0. 05. After treatment compared with the model group the 24hUTP Scr and TGF-β1 of the TCM group were significantly decreased P < 0. 05 but still higher than the normal group P < 0. 05. After 8 weeks of drug treatment the pathological damage of the kidney in the Chinese medicine group was significantly reduced compared with the model group. The glomerular volume is smaller than the model group. Compared with the model group some of the basement membrane thickened the mesangial matrix increased and the infiltration of inflammatory cells in the glomeruli was reduced. Conclusion Yiqi Yangyin Tongluo Prescription can significantly improve the renal function of DN rats and delay the progression of DN. The mechanism may be related to the reduction of 24hUTP serum creatinine and down-regulation of TGFβ1 protein expression in DN rats. Reference citation DU Zhenfang QIANG Sheng HUANG Min et al. Effect of Yiqi Yangyin Tongluo Prescription on TGF-β1 Expression in Model Rats with Diabetic Nephropathy J. Acta Chinese Medicine 2018 33 11 2094-2098. Key words diabetic nephropathy Yiqi Yangyin Tongluo Prescription TGF-β1 renal function 24 hour urine protein quantification serum creatinine transforming growth factor-β1 rat 2 h 12 h 7 d 1 diabetic nephropathy DN 30 g 10 g 20 g 10 g 1. 5 g 15 g 15 g DN 2 g ml - 1 extracellular STZ Sigma TGF-β1 matrix ECM - Santa Curz DN 2-3 - 1. 3 β1 transforming growth factor-β1 TGF-β1 STZ DN SD 1 10 ECM ECM 40-4-5 63 4 30 20 2 DN 6 8 STZ 6 DN TGF-β1 40 mg kg - 1 72 h 24 h DN 24 urine protein quantitation DN in 24 hours 24hUTP 16. 7 mmol L - 1 > 150% 24hUTP > 30 1 mg 2 DN 1. 1 SD 100 1. 4 SCXK 2 g ml - 1 2013-0006 180 ~ 220 g 6 ~ 9 22 ~ 25 50% ~ 70% 12 1. 2 32. 4 ml 64. 8 g 64. 8 ml 129. 6 g 129. 6 ml 259. 2 g 200 ml 2095
3 1 9 PBS 4 5 000 g 5 ~ 10 min SD 10 ELISA TGF-β1 10 1. 6 SPSS 18. 0 10 8 ± x 珋 ± s t P < 0. 05 g ml 2 8 2. 1 1. 5 1 STZ 224hUTP 1 1 8 16 24 h 24hUTP 7 3 12 h 4 h 2 1 8 200 g 1 ~ 2 ml 1 2 1 2 SD 4 ~ 5 ml2. 2 1 h 3 000 r min - 1 4 10 min 7020 40 g L - 1 4 TGF-β1 PBS 0. 01 mol L - 1 ph 7. 4 1 A B C D E 1 HE 200 2. 3 24hUTP 2. 4 8 8 P < 0. 05 P < 0. 05 16 16 P < P < 0. 05 0. 05 1 2 2096
2. 5 TGF-β1 ELISA 3 TGF-β1 OD y = 82. 898x 2 + 180. 72x + 1. 344 r = 0. 999 9 1. 000 ~ 15. 625 ng L - 1 2 1 24hUTP x 珋 ± s mg n 1 8 16 10 2. 32 ± 0. 34 2. 46 ± 4. 52 3. 01 ± 4. 05 8 3. 24 ± 0. 20 35. 74 ± 5. 78 * 32. 47 ± 8. 23 * 8 1. 90 ± 0. 19 34. 15 ± 4. 82 * 6. 81 ± 7. 02 * 9 1. 35 ± 0. 73 33. 73 ± 5. 56 * 8. 20 ± 6. 62 * 9 2. 29 ± 0. 28 34. 83 ± 4. 02 * 12. 78 ± 6. 74 * * P < 0. 05 P < 0. 2 x 珋 ± s μmol L - 1 n 1 8 16 10 48. 57 ± 1. 34 45. 75 ± 2. 01 43. 32 ± 0. 31 8 43. 64 ± 0. 86 104. 10 ± 0. 09 * 112. 34 ± 2. 20 * 8 49. 10 ± 0. 75 98. 97 ± 1. 29 * 52. 84 ± 1. 95 9 45. 62 ± 1. 35 100. 02 ± 0. 83 * 60. 71 ± 0. 62 * 9 47. 75 ± 0. 94 96. 53 ± 1. 02 * 75. 04 ± 0. 89 * * P < 0. 05 P < 0. 2 TGF-β1 OD OD DN TGF-β TGFβ1 Ranjan 12 DN TGF-β1 TGF-β1 P < TGF-β1 TGF-β 11 0. 05 DN TGF-β1 TGF-β1 TGF-β1 P < 0. 05 3 TGF-β1 n TGF-β1 x 珋 ± s ng L - 1 10 54. 63 ± 0. 72 8 88. 83 ± 0. 26 * 8 60. 38 ± 0. 48 * # 9 64. 66 ± 0. 85 * # 9 71. 35 ± 0. 97 * # * P < 0. 05 #P < 0. 3 DN DN DN 8 DN 9-10 DN DN 2097
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