Tetranychus urticae Koch (Acari: Tetranychidae)

42 1 1390 (7583) : * : 09111438400 Email: mghadamyari@gmail.com Tetranychus urticae Koch (Acari: Tetranychidae) 1 2 * 3 4 1 2 3 4 ) : 89/8/15 : (90/3/24 Tetranychus urticae Koch... (EC 25%). 30<) ( 3000. 2/14 1/33. 1/71 K m V max CDNB 1/43 1/15. P 450 1/37.. :.P 450 Tetranychus urticae Koch Tetranychidae. 0

76 42 1390 1.(Jeppson et al., 1975)..(Herron et al., 1998).(Jeppson et al., 1975) (Brown,.03). %90 ) ( ) ( (Feyereisen, 1995; Roush &.Tabashnik, 1990). 50.(Devorshak & Roe, 1998) ) (.. P 450. (Pickett & Lu,.1989; Prapanthadara et al., 1993) (Mosalanejhad et al., 02)..(Scott, 1995).. Convolvulus sp.. T. urticae. (Phaseolus vulgaris).

: Tetranychus urticae... 77. 15 24. 24±2 70± 16:8 ) : ( 90. (LeafDip Bioassay) (Cahill et al., 1995;.Morin et al., 02) ) (.. ) 3/5 (. 45. 24±2 70± 16:8 ) : (. 48.. (αna) TEMED (GSH) CDNB (1chloro2,4dinitrobenzene) () ) (. 3, 3`, 5, 5` tetramethyl benzidine (TMBZ). Panreac 0 0/1 ph 7 (NaH 2 PO 4 + Na 2 HPO 4 ) X0 (v/v) MFO 000g 15. (Supernatant). van Asperen (1962). 4 18. 12/5 25.. (STAT FAX 30) 450..

78 42 1390 1 nmol/min/mg protein. CDNB Habig et al. (1974). 25 GSH ( 0) CDNB 50) ( 2 7 ph) 0/1 ( 340 (Kinetic) STAT. FAX 30 15 ) ph) (7 135 50 CDNB ) 0/05 0/1 0/2 0/4 0/6 0/8 1 1/6 ( 0 GSH ) ( 340. K m V max.. P 450 hemeperoxidase.(brogdon et al., 1997) 80 0/625 ph) 0 (7/2 3, 3`, 5, 5` tetramethyl benzidine (TMBZ) 25 H 2 O 2 (%3). 2 450. C. Bradford (1976).. SAS t.... Wang & Wu (07) Bemisia tabaci Genn... 1..(Leora Software, 1978). ) ppm) ((30 ).( 50 3000.

79... Tetranychus urticae : (1995) Campos et al.. ppm 0/1 (Leaf Residue Assay). 242 125. ppm Stumpf & Nauen 1/06 COL00 ppm NL00 (02) 0/04 26 54 95 50 1 b (%95 ) >>3000 χ 2 c (df) 0 /56(3) SE± 0/22 ± 1/08 %95 LC50 a (%95 ) d 1 /06 ( 0/ 72 2 / 06) 290 %95 (ppm) LC50 LC50 a =(Relative Potency) b c. χ 2 %5 χ 2 d. LC50 (02) Stumpf & Nauen. Stumpf & (02) Nauen.. (αna). (αnp) %1.(2 ) (09) Lin et al.. T. cinnabarinus 2/7 (06) Wang & Wu. B. tabaci. 2 (nmol/min/mg protein) ±SE ( /) ** 2 /14± 0/ 187 609 /8± 8/ 35 289 /16± 27/ 82 αna 1 /33± 0/ 001 ** 569 /64± / 60 428 /92± 4/ 81. %5 αnp **

1390 1 42 80 V max. 92/47±1/81 1/15. V max. Sitophilus zeamais K m GSH CDNB 2 V max. 2 GSH CDNB. DCNB GSH. Sitophilus zeamais.(fragoso et al., 07) K m (04) Konanz & Nauen GST V max (GSS WI) (NL00). GSH CDNB K m WI GSH CDNB 90 75 79 NL00 GSS 2 V max (GSS) (Konanz &.Nauen, 04). CDNB 1/71.(1 ) (02) Stumpf & Nauen GST P 450 11 13. Stumpf &. (02) Nauen 3/4. (02) Stumpf & Nauen GST T. cinnabarinus (09) Lin et al. Wang & Wu. 2 (06). B. tabaci V max K m V max /K m V max K m. 3 CDNB.. K m 1/43 K m 3 V max /K m V max (mm/min) K m (mm) 1844 /4± 1 / 9 80 /09± 2/ 22 0 /043± 0/ 002 3064 /5± 174/ 13 ** 92 /47± 1/ 81 * 0 /030± 0/ 001 *. %5 *

81... Tetranychus urticae : van Leeuwen et al. TMBZ 2 (06).. 2/14 P 450 %1.(2 ) 1/37. P 450 (07) Enayati et al. Blattella germanica L. P 450 1/58 4/6. (nmol/min/mite) 80 70 60 50 40 30 0 1 (units of cytochrome p450/mg protein) 0.0007 0.0006 0.0005 0.0004 0.0003 0.0002 0.0001 0 P 450 2

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