29 4 2003 7 551 556 ACTA AGRONOMICA SINICA Vol. 29, No. 4 pp. 551 556 July, 2003 (, 150030) 62,, ;,, 19 3, 9 6, Ξ, ; ; ; : S565 : A Composition Analysis of Protein and Oil and Amino Acids of the Soybean Varieties in Heilongjiang Province of China NING Hai2Long LI Wen2Xia WANG Ji2An CHEN Qing2Shan KONG Fan2Jiang ZHANG Shu2Zhen YANG Qing2Kai ( Soybean Research Institute, Northeast Agricultural University, Harbin, Heilongjiang 150030, China) Abstract The seed nutrition quality of 62 soybean varieties in the Northeast China were analyzed in this paper. For the variation coefficient of seed nutrition of amino acids, proline and glycine took the first place and last place, respectively. But the parameter for protein and fat content were very stable among varieties. According to principle component analysis, in which 3 principle components were selected from 19 to evaluate the qualities further, 9 varieties were screened out for their better integrated properties. By clustering analysis all varieties were divided into 6 groups,which were different in quality. Parents used in cross breeding should be selected from different clusters considering the complementarities of prin2 ciple components. Key words Soybean ;Quality properties ; Principle components ;Clustering analysis, [1,2 ], [8 ],,, ; [3 7 ] ;,,,, Ξ : (30130120) 863 (2001AA211041) : (1975 - ),,,, Received( ),2002205224,Accepted( ) 20022102291
552 29 1 111 17, 62 ( 1) Perton28620 1. 2, 3 2, 113 0165 m, 0108 m, 6 m 4 28,9 3 25 10 Excel SAS Statistica Table 1 1 Soybean cultivars tested in this experiment No. Name No. Name No. Name No. Name 1 8647 8647 17 7828 ND 7828 33 9303 JN 9303 49 962970 H 962970 2 3 JF 3 18 808421 G 808421 34 9473 JN 9473 50 892629 G 892629 3 19 HH 19 19 4 SN 4 35 7714 JN 7714 51 9623238 K 9623238 4 454 B 454 20 8 SN 8 36 956821224 JN 956821224 52 9725293 S 9725293 5 5 HF 5 21 14 SN 14 37 910721 GJ 910721 53 9725033 S 9725033 6 18 FSH 18 22 15 SN 15 38 7335 GJ 7335 54 918222 G9 18222 7 9 HF 9 23 932355 S 932355 39 E3147 E 3147 55 D942841 KD 942841 8 932111 H 932111 24 9525672 S 9525672 40 4 XY 4 56 952865 HJ 952865 9 972402 K 972402 25 38 DN 38 41 5 XY 5 57 3049121 DN 3049121 10 9823910 K 9823910 26 434 DN 434 42 6 XY 6 58 163 DN 163 11 85423 85423 27 962868 DN 962868 43 982903 SHN 982903 59 5976 ND 5976 12 85425 85425 28 823 DN 823 44 301 DN 301 60 97402 K 97402 13 85426 85426 29 9228033 DN 9228033 45 5129 ND 5129 61 42 DN 42 14 854210 854210 30 922070 DN 922070 46 4900 ND 4900 62 L13 15 854211 854211 31 932046 DN 932046 47 9726630 H 9726630 16 3360 ND 3360 32 22 JN 22 48 118 BY 118 DN L13 2 211 2 :,, ; 8106,, 6138 :,, 15 ;, 43113 %, 20176 %,,, 14, ;, Table 2 2 Variation of quality property in different soybean Quality properties Range Average CV Cys 0149 0197 017250 0112978 Met 01440 01930 0165069 0114417 Asp 21979 61518 4161940 011197 Thr 01993 21211 1157215 011179 Ser 11353 31041 2111432 011229 Glu 11747 101225 7111827 011553 Gly 01997 21229 1159715 011178 Ala 01746 21749 1161556 011650 Val 11193 21695 1186842 011226 Ile 11169 21423 1177171 011262 Leu 11838 41216 2192458 011195 Tyr 01599 11504 1102952 011683 Phe 11261 21919 1196961 011211 Lys 11422 31562 2139440 011494 His 01669 11634 1108514 011266 Arg 11474 41487 2187079 011450 Pro 01511 21042 1101853 012193 Protein 38172 46178 4311374 0104087 Oil 17152 23190 2017642 0106125
4 : 553 212 19,, ( ) (A),,,,, 19 3 1 2 3, 9188 % 3 A 1 A 2 A 3 3, 8712 %, 3, 19 3 Quality properties A 1 A 2 A 3 Cys 01233 01124-0111 Met 0124 01067 01024 Asp 01263-0104 01083 Thr 01262 0102 01167 Ser 01258-01074 01156 Glu 01221-0101 01086 Gly 01261-01016 01141 Ala 01223 01061 01053 Val 01264 01084-01076 Ile 01252 01136-01098 Leu 01267 01012 01074 Tyr 01161-01197 01674 Phe 01261 01041-01059 Lys 01231 01199-01298 His 01257 01049-01135 Arg 01248-01142 - 01058 Pro 01172 01001-01454 Protein 01069-01668 - 01057 Oil - 01069 01628 01315 Principal components The first The second The third 2 Amino acids Name Protein oil factor factor Tyr2Pro factor 71182 8117 8712 Cumulative percent ( %) 42115 %,,,, 3 5155 %, Table 3 Principal component analysis of quality properties in soybean 2 1 2 3 Eigenvalues 131645 11873 11054 Eigenvector,,,,,,,,,,,,,,, 62 3 ( 4) 4 gz 1 = 101511 S 1 = 11161, gz 2 = - 151516 S 2 = 11911, gz 3 = 41906 S 3 = 01507, : : Z 1 > 111511,111511 Z 1 < 91511, Z 1 91511 ; : Z 2 > - 131516 Z 2 < - 171516, - 161516 Z 2 < 141516, ; : Z 3 > 51706 Z 3 41106, 41506 < Z 3 51306, 4,3 2 : 3, 4 ( 301),48 ( 118) ; 2, 15,, 8 ; 17,
554 29 Table 4 4 Principal component value of soybean varieties 3 No1 Z 1 Z 2 Z 3 No1 Z 1 Z 2 Z 3 Evaluation 3 Evaluation 1 91704-141908 41942 1b2c3c 32 101556-141575 41631 1b2c3c 2 9181-141885 41912 1b2c3c 33 101127-151138 41955 1b2c3c 3 101624-141072 515 1b2a3b 34 10187-131515 41261 1b2a3b 4 111497-151088 5134 1b2c3b 35 101489-121548 41955 1b2a3c 5 101319-16188 41509 1b2c3c 36 101731-111834 51716 1b2a3a 6 81361-161462 41489 1c2c3b 37 101264-151335 41958 1b2c3c 7 11163-131119 51681 1a2a3b 38 91965-151125 41794 1a2c3c 8 121102-131552 51551 1a2b3b 39 91989-131479 41861 1b2a3c 9 71233-141574 4187 1c2c3a 40 101755-131397 51722 1b2a3a 10 111617-151495 41877 1a2c3c 41 111422-17188 4156 1b2a3c 11 81083-131636 51317 1c2b3b 42 111134-14171 51327 1b2c3b 12 101861-161402 41746 1b2c3c 43 121412-151374 51146 1a2c3c 13 111342-181177 41207 1b2a3b 44 151005-191909 31969 1a2a3a 14 101796-181448 41067 1b2a3a 45 101095-151969 51108 1b2c3c 15 101568-151888 4118 1b2c3b 46 81198-141502 41967 1c2b3c 16 101802-171466 41695 1b2b3c 47 91394-141142 51263 1c2b3c 17 91864-151899 5114 1b2c3c 48 121359-18194 3183 1a2a3a 18 9168-151544 41815 1b2c3c 49 111713-181344 41544 1a2a3b 19 101601-161692 41826 1b2b3c 50 10115-151505 51046 1b2c3c 20 101846-151945 51187 1b2c3c 51 101464-161911 41541 1b2b3c 21 111041-161774 51012 1b2b3c 52 111355-151008 5135 1b2c3b 22 111392-16131 51042 1b2c3c 53 101124-181009 41532 1b2a3c 23 91498-171467 41433 1c2b3b 54 9187-131065 51241 1b2a3c 24 111136-17147 41556 1b2b3b 55 121143-151993 41777 1a2c3c 25 101226-131821 51522 1b2b3b 56 91616-151291 41855 1c2c3 26 101061-121338 61084 1b2a3a 57 101377-131056 51646 1b2a3b 27 91102-151371 41551 1c2c3c 58 91967-131752 51026 1b2b3c 28 101917-181049 41662 1b2a3c 59 111315-171475 31769 1b2b3a 29 91558-101598 6121 1b2a3a 60 91755-131802 51349 1b2b3b 30 3 111532-181028 41377 1a2a3b 61 101132-161954 41665 1b2b3c 31 91877-151685 41969 1b2c3c 62 101255-171441 41546 1b2b3c 3 : a b c 3 : a,b,c mean good,middle and bad respectively. 213 ( 1), 6, ( 5), :
4 : 555 Fig11 1 62 Clustering analysis of 62 soybean varieties in Heilongjiang province Table 5 5 Average and standard deviation of quality properties in different clusters Quality properties Average SD Average SD Average SD Average SD Average SD Average SD Cys 01761 01090 01718 01082 01831 01070 01736 01074 01575 01063 01677 01401 Met 01715 01080 01632 01072 01770 01056 01654 01043 01487 01029 01598 01069 Asp 51044 01521 41062 01344 51283 01234 41505 01229 31617 01701 41351 01170 Thr 11693 01196 11555 01122 11799 01071 11557 01087 11239 01217 11485 01055 Ser 21339 01248 21093 01168 21414 01126 21061 01114 11658 01299 11980 01045 Glu 71885 01831 71164 01524 81236 01397 71045 01381 41508 11575 61795 01235 Gly 11732 01172 11584 01118 11835 01082 11565 01084 11249 01228 11514 01061 Ala 11811 01242 11561 01257 11860 01092 11646 01110 11097 01219 31526 01071 Val 21028 01240 11829 01518 21114 01105 11880 01116 11452 01231 11767 01113 Ile 11888 01191 11734 01179 21064 01137 11775 01144 11419 01219 11660 01124 Leu 31180 01370 21897 01208 31330 01163 21896 01150 21273 01388 21751 01104 Tyr 11116 01164 11036 01140 11198 01094 01963 01206 01865 01120 01978 01114 Phe 21148 01287 11947 01161 21193 01114 11973 01107 11571 01254 11835 01117 Lys 21549 01405 21341 01283 21677 01157 21506 01302 11828 01315 21221 01199 His 11198 01171 11058 01100 11209 01054 11088 01056 01866 01114 11017 01059 Arg 31308 01450 21833 01188 31169 01195 21790 01187 21121 01567 21714 01144 Pro 11178 01337 01963 01120 11178 01229 11006 01129 01769 01164 01957 01157 Protein 45149 01604 44132 01558 42149 01869 40197 11028 42147 01869 42139 01441 Oil 19103 01803 20138 01713 21145 01762 22121 01860 21110 01740 20158 01261 1 : 11, 19174 % 25181 %, ;, ;, 2 : 16, 3 : 7,
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