2 011 2 6 2 4 8-252 NS 1 1 1 1 1 1 2 2 2 3 3 4 4 4 1. 530001 2. 100101 3. 530004 4. 530001 /28 90 8. 99 ± 1. 60 kg 3 3 A B 0. 2% m /m NS C 0. 2% m /m 0. 025% m /m 0. 012 5% m /m 3 30 d NS NS A 9. 12% 7. 64% 7. 67% P < 0. 05 9. 70% P < 0. 05 50% P < 0. 01 B GLU A 20% SOD 10. 9% P < 0. 05 NS S828 A 1000-7091 2011-0248 - 05 The Effects of NS-lactobacillus Complex on Productivity Performance Plasma Biochemical Indexes and Dilarrhea Rate in Weaned Piglets HE Ying 1 ZHAO Wu 1 CHEN Zhong-wei 1 QIN Yi-bin 1 LIANG Bao-zhong 1 LIANG Jia-xing 1 HU Xu 2 JIN Feng 2 SUN Zhi-yong 2 HU Ting-jun 3 LAI Sheng-ji 3 WEI Ming-yu 4 ZHANG Heng-bo 4 LAN Bing-shun 4 1. Guangxi Veterinary Research Institute Guangxi Key Laboratory of Animal Vaccines and New Technology Nanning China 2. Key Laboratory of Mental Health Institute of Psychology Chinese Academy of Sciences Beijing 3. University of Guangxi Nanming 530004 China 4. Livestock Research Institute of Guangxi Nanning 053001 100101 China 053001 China Abstract A total 90 of crossbred piglets 1 /2 Large white 1 /2 Duroc 28-day-old 8. 99 ± 1. 60 kg BW were divided into 3 groups by weight and gender with 3 replicates in each group and 10 piglets in each replicate. The 3 groups were as follows control group A fed corn-soybean basal diet NS-Lactobacillus group B added 0. 2% m /m NS-Lactobacillus complex in basal diet antibiotic group C added 0. 2% m /m aureomycin 0. 025% m /m amoxicillin and 0. 012 5% m /m tiamulin in basal diet. The experiments included 30 days. The productivity performance blood biochemical indexes and the diarrhea rate were assayed. The results showed as follows the average final weight average weight gain and average daily gain in NS-Lactobacillus group were improved by 9. 12% 7. 64% and 7. 67% compared with control group P < 0. 05 the feed conversion and diarrhea rate in NS-Lactobacillus group were decreased by 9. 70%. and 50% P < 0. 01 In NS-Lactobacillus group blood GLU level and SOD activity were improved by 20% and 10. 9% compared with control group P < 0. 05 which indi- 2011-08 - 30 0992014-5 1979-1982 - 1965-1956 -
NS 249 cates the anti-oxidation and stress reducing effect of NS-Lactobacillus. Key words NS-Lactobacillus complex Piglets Growth performance Blood biochemical parameters The diarrhea rate 1 1. 1 1 1. 1. 1 20 8. 99 ± 1. 60 kg / 90 3 ge 14 2 1. 1. 2 NS 10 9 10 8 10 5 10 5 10 8 1. 1. 3 100 ppm 450 mg /g NS 100619 100607 870 mg /g 1008304033 1. 1. 4 1 NS / Composition / content Tab. 1 1 Composition and nutrient level of basal diet Creep feed Nursery feed / Composition / content Creep feed Nursery feed /% Corn 51 53 /% Fatty powder - 2. 5 /% Bean pulp 9 21 K81 # /% Concentrate feed #K81-19. 43 K76 # /% Concentrate feed #K76 39. 76 - /% Feed additives 0. 24 0. 57 /% Improted fish powder - 3 /% Summation 100 100 / MJ /kg Digestive energy 3242 3232 /% 19. 31 18. 43 * A 8 500 U D1 300U E 12 U K 2. 0 mg B1 1. 2 mg B24. 0 mg B6 0. 2 mg 10. 0 mg 600 mg 16. 0 mg 0. 3 mg 0. 06 mg 220 mg 100 mg 60 mg 120 mg 0. 3 mg 0. 3 mg Note * mean that it will supply Vitamin A 8 500 U Vitamin D 1 300 U Vitamin E 12 U Vitamin K 2. 0 mg Vitamin B1 1. 2 mg Vitamin B 24. 0 mg Vitamin B6 0. 2 mg Calcium pantothenate 10. 0 mg choline 600 mg Niacin 16. 0 mg Folate 0. 3 mg Biotin 0. 06 mg Cu 220 mg Fe 100 mg Mn 60 mg Zn 120 mg Se 0. 3 mg I 0. 3 mg for the basic ration. 1. 1. 5 AST ALT BUN Cr TC TG GLU TP ALB 3 10 3 A B C A ALP SOD 1 B 0. 2% m /m NS CE 0. 2% m /m 0. 025% m /m 0. 012 5% m /m 1. 2 90 3 30 d 1. 2. 2 0 15 30 28 33 58 1. 2. 1 3 3 ml
250 26 9 37 30 min CE - 20 1. 2. 3 0 30 16 h 1. 3 SPSS17. 0 1. 2. 4 8 00 ± N = / 100% 2. 1 NS 1. 2. 5 OLYMPU- SAU-400 15 ~ 30 d B AST ALT BUN KY-2000 Duncan's α = 0. 05 2 GLU TP ALB A 9. 12% 7. 64% 7. 67% ALP 9. 70% P < 0. 05 SOD 1 0 ~ 15 d A 13. 03% P < 0. 01 Cr TC TG 30 d B Tab. 2 2 Each group of growth performanee the raising test result Composition A A Group B B Group C C Group Number of threads 30 30 30 / d Proving time 30 30 30 /kg Average weight of begin 8. 98 ± 1. 75 9. 01 ± 1. 77 8. 98 ± 1. 27 15 d /kg 15 d average weight 15. 65 ± 2. 59 15. 79 ± 2. 60 15. 70 ± 1. 93 /kg Average weight of ends 23. 25 ± 3. 44 25. 37 ± 3. 35 a 23. 79 ± 3. 12 15 d /kg Dynamiting of 0 d to 15 d 6. 67 ± 1. 25 6. 77 ± 1. 28 6. 72 ± 0. 98 15 d /kg Dynamiting of 16 d to 30 d 7. 60 ± 1. 17 8. 59 ± 1. 42 A 8. 09 ± 1. 02 /kg Average dynamiting 14. 27 ± 2. 07 15. 36 ± 1. 96 a 14. 81 ± 1. 57 /g Average daily gain 475. 51 ± 69. 12 512. 10 ± 65. 25 a 493. 63 ± 52. 31 FCR 1. 65 ± 0. 10 1. 49 ± 0. 64 a 1. 51 ± 0. 44 ± x ± SD 0. 01 a A A marked with capitalized letter are extreamly different P < 0. 01. The a and A means that data in the row compare with the A group are significantly different or extreamly different. 2. 2 NS C A 3 B 25% P < 0. 05 B C A P < 0. 01 50% C 3 Tab. 3 33. 35% P < 0. 05 Effect of the group on diarrheal of piglets /% /% Group Total of piglets Diarrhoea frequency Mortality Rate of diarrhoea Rate of mortality A A Group 30 145 1 26. 67 ± 1. 5 3. 33 B B Group 30 64 0 13. 33 ± 1. 1Ac 0 C C Group 30 89 0 20. 0 ± 1. 0 ab 0 ± x ± SD 0. 01 a A A b B B c C C marked with capitalized letter are extreamly different P < 0. 01. The a and A means that data in the group compare with the A group are significantly different or extreamly different The b and B means that data in the group compare with the B group are significantly different or extreamly different The c and C means that data in the group compare with the C group are extreamly different.
NS 251 2. 3 NS 30 d B GLU 0 d 16. 5% P < 0. 01 30 d A 3 30 d 0 d ALP Cr TG A B C P < 0. 05 30 d NS Cr ALT 55. 8% 92. 2% 71. 1% P < 0. 01 53. 1% 63. 7% 41. 7% P < 0. 01 TC Cr CE P < 0. 05 30 d B TP 37. 6% 48. 1% 39. 2% A B GLU A P < 0. 01 ALB 20% SOD 10. 9% 28. 2% 29. 2% 30. 9% P < 0. 01 NH 3 14. 3% 19. 8% 16. 1% P < 0. 01 30 d A TG 0 d 29. 3% P < 0. 05 Biochemical Unit indicator Tab. 4 4 GLU P < 0. 01 30 d C P < 0. 05 30 d C ALP A 41. 1% P < 0. 05 CE A 124. 1% P < 0. 01 Effect of the LactobacilIus on plasma biochemical indexes of piglet A A Group 0 d 30 d B B Group C C Group A A Group B B Group C C Group AST U /L 60. 46 ± 22. 89 59. 91 ± 14. 27 58. 20 ± 25. 16 60. 64 ± 25. 79 67. 12 ± 19. 39 73. 46 ± 29. 30 ALT U /L 41. 81 ± 7. 18 41. 07 ± 7. 48 44. 90 ± 6. 12 65. 13 ± 17. 74 A 78. 94 ± 22. 22 A 76. 84 ± 23. 0 A ALP U /L 67. 19 ± 16. 41 75. 63 ± 15. 0 80. 37 ± 34. 23 48. 89 ± 7. 82 A 63. 65 ± 7. 89 69. 0 ± 17. 32 b BUN mmol /L 2. 22 ± 0. 69 2. 75 ± 1. 48 2. 43 ± 0. 36 3. 02 ± 0. 66 3. 07 ± 1. 16 3. 01 ± 0. 60 Cr μmol /L 90. 18 ± 24. 20 88. 50 ± 14. 32 96. 30 ± 15. 16 109. 51 ± 11. 08A 118. 17 ± 18. 03 A 114. 14 ± 19. 89 TC mmol /L 2. 07 ± 0. 34 2. 02 ± 0. 46 2. 06 ± 0. 26 3. 17 ± 0. 39 A 3. 31 ± 0. 34 A 2. 92 ± 0. 51 A TG mmol /L 0. 58 ± 0. 19 0. 57 ± 0. 15 0. 63 ± 0. 12 0. 75 ± 0. 23 A 0. 68 ± 0. 20 0. 68 ± 0. 84 GLU mmol /L 7. 03 ± 0. 54 7. 60 ± 0. 57 7. 43 ± 1. 17 7. 95 ± 1. 36 9. 54 ± 1. 93 A b 7. 51 ± 1. 70 TP g /L 43. 59 ± 8. 34 44. 26 ± 7. 56 42. 91 ± 3. 87 59. 97 ± 3. 57 A 65. 57 ± 8. 98 A 59. 74 ± 12. 59 A ALB g /L 32. 04 ± 6. 06 32. 89 ± 5. 74 31. 51 ± 3. 23 41. 08 ± 3. 52 A 42. 49 ± 3. 96 A 41. 24 ± 8. 27 A SOD U /L 96. 30 ± 22. 04 97. 92 ± 6. 32 95. 92 ± 6. 18 94. 71 ± 7. 71 105. 07 ± 5. 29b 92. 05 ± 10. 98 CE EU 0. 63 ± 0. 29 0. 57 ± 0. 28 0. 84 ± 0. 35 0. 54 ± 0. 21 0. 89 ± 0. 34 1. 21 ± 0. 87 B NH3 mmol /L 65. 31 ± 8. 22 68. 54 ± 10. 15 66. 13 ± 13. 81 55. 94 ± 6. 47 A 54. 94 ± 6. 47 A 55. 49 ± 5. 33 A ± x ± SD 0. 01 a A 0 d b B 30 d 30 d A marked with capitalized letter are extreamly different P < 0. 01. The a and A means that data in the group compare with the same group in the 0 day are significantly different or extreamly different The b and B means that data in the group compare with the A group in the 30 day are significantly different or extreamly different. 3 ph D 3 3. 1 NS NS 3. 2 NS NS 15 d NS 15 d 4 NS Gibson 5
252 26 C A B 124% 36% ph G - 3. 3 NS 12 3 30 d C ALP A 41. 13% NS ph ALT AST G - BUN 6 ALT AST IgA ALP Yablanski 7 8 4 NS 30 d A 20% NS SOD A NS 9 20% 1. J. 2006 6 7 102-103. 2. SOD J. 2001 3 1 45-47. 3. J. 2004 16 6 378-379. 10 11 4 Barro P A. The attachment of bacteria to the gastric epithelium of the pig and its importance in the microecology B of the intestine J. J Appl Bacteria1 1980 48 9 147-154. 5 Gibson G R. Regulatory effects of bifidobacteria on the growth of other colonic bacteria J. J Appl Bacterial 1994 77 6 412-420. 6. NS J. 2002 17 1 86-90. SOD 7. A NS J. 2001 1 34-37. 8. J. 1999 1 34-36. 9. α- 3. 4 NS J. 2010 31 16 23-25. 10 Balázs Borsiczky Zsolt Szabó Mohammad T et al. Activated PMNs lead to oxidative stress on chondrocytes J. Acta Orthop Scand 2003 74 2 190-195. G - 11. J. 2008 40 5 5-8. 12 Khinev S Tsoneva D Dafinova K et al. Bacterial translocation from the gastrointestinal tract. catalyst of multiple organ dysfunction syndrome J. Khirurgiia 2000 56 30 d 2 20-23.