(, 100021) : (), Ab 2 (internal image), :, ; ; Advances in Research of Anti2idiotypic Antibody LI Min, J I Rong (National Institute for Nutrition and Food Safety, Chinese CDC, Beijing 100021, China) Abstract : Anti2idiotypic antibody is a kind of special antibody activated by antigenic determinant of antibodyπs variable region. The anti2idiotypic antibody Ab 2has been applied to a number of domains of biological research such as vaccine development, tumor immunity, transplantation tolerance, autoimmune diseases and food safety, because it is the internal image of initial antigen in the body and can simulate the initial antigen. The recent advances in the research of anti2idiotypic antibody were introduced. Key word : Antibody, Anti2idiotypic ; Antigens ; Allergy and Immunology Jerne 1974 (immune network theory),,,, (antibody,ab 1 ), 2, (Ab 2 ), 3 (Ab 3 ),4 (Ab 4 ),,,,,,,,, (idiotype,id),, Id (V L ) (V H ),,(idiotope),,(anti2idiotypic antibody, AId) 1 AId Id AId,AId Ab 2 Ab 2 Ab 2 Ab 24 [1 ] Ab 2 Ab 1,Ab 2 Ab 1 Ab 1, Ab 2 Ab 2 Ab 1, Id,, (internal image) Ab 2: (1) Ab 1 ; (2) Ab 1 ; (3) Ab 2 Ab 1, ; (4),Ab 2 Ab 2 Ab 1 : :, Ab 1, Ab 2 Ab 2 Ab 1 56 2006 18 1
, 2 Ab 2 Ab 2,, Ab 2 Ab 2 2 2. 1 2. 111 AId AId,,,, AId Ab 1, 2001 000gΠ,,, AId,,,,, AId, [2 ] 21112,,,, Ab 1,Ab 1,,, Fc,,,,,, [3 ] Ab 1,,Ab 1 Fab (fragment antigen binding,fab) [4 ] Ab 1 Fab, Ab 1 Fab [5 ] Fab,(N2ethyl2 42vinylpyridinium bromide),,,(methacrylic), ph Fab, Fab,Fab,Fab A G,Gradiflow Fab [6,7 ], Fab,Fab Fc IgG,,, Fab Fc 50 kda, ;Fab IgG Gradiflow Fab, 10 min Fab Gradiflow IgG F ( ab ) 2 [7 ] AId,20 %,1 %, Ab 1 [3,8 ],Ab 1, 212 AId 2 : AId ;DNA AId, AId, AId (phage display technology) 1985, DNA,, [9 ] AId BalBΠc, mrna,rt - PCR V H V L cdna(complementary DNA,cDNA), DNA ScFv ( single2chain fragment variable) DNA,ScFv DNA DNA, ScFv, ScFv [10 ], [11 ],AId,, 57
3 Ab 2 311 31111 ( enzyme2linked immunosorbent assay, ELISA) - Ab 2 [12 ], Ab 2, AId,AId ( ),Ab 2 (AId ),, 450 nm 490 nm () ( A),,AId, AId Ab 1 37 1 h, Ab 1,ELISA A A Ab 1,, [13 ] 31112 (flow cytometry method,fcm) Ab 2,Ab 2, Ab 2 [14 ] 31113 ( immunohistochemical localization),, Ab 2,,Ab 2, Ab 2 [15 ] 312 Ab 2 (Ab 2 ) BalBΠc,(50 d 65 d), ELISA,Ab 2 [3,15 ] Ab 3, Ab 3 Ab 1, Ab 2 AId [16 ] Ab 2, Ab 2,,, Ab 2 4 Ab 2 411 Ab 2,, [17 ],, [18 ],,, Cp G CFA [19 ] ;- 6 ( IL - 6), IL - 6 B, B, [20 ],, ( single2chain fragment variable, ScFv),AId ScFv,, [21 ] 412 Ab 2,,, (major histocompatibility complex,mhc) Ab 2 MHC Ab 2,, [22 ],Ab 2, [23,24 ] 413 Ab 2 Ab 2,, ( human anti2mouse antibody, HAMA),,,, [25 ], 58 2006 18 1
Ab 2,, Ab 2 ScFv DNA, [26 ], ScFv,,, [22,27 ] 414 Ab 2 [28 ] AId,,, AId 415,,,,,,,,,,,,,68 h,,,,,,, [1 ],. [ M].., 2003. 27. [2 ]. [J ].,1999,14 (4) :92295. [3 ],,,. [J ]., 2000,16 (6) :4812483. [4 ] Gaulton G N, Sharpe A H, Chang D W, et al. Syngeneic monoclonal internal image anti2idiotypes as prophylactc vaccines[j ]. Immunology,1986,137 (9) :293022936. [5 ] Dainiak M B, Muronetz V I, Izumrudov V A, et al. Production of fab fragments of monoclonal antibodies using polyelectrolyte complexes [ J ]. Analytical Biochemistry, 2000,277 :58266. [6 ] Coleman L, Mahler S M. Purification of Fab fragments from a monoclonal antibody papain digest by Gradiflow electrophoresis [ J ]. Protein Expression and Purification, 2003,32 :2462251. [7 ] Cheung L M, Thomas T M, Rylatt D B. Purification of antibody Fab and F ( abπ) 2 fragments using gradiflow technology[j ]. Protein Expression and Purification,2003,32 : 1352140. [8 ],. [J ].,1993,9 (4) :46250. [9 ],,. [M]. :,2001. 1092127. [10 ],. [J ].,2001,17 (6) :7862789. [11 ],,,. [J ]., 2003,28 (1) :28230. [12 ],,,.. HbcAb [J ].,1991,5 (2) :1862189. [13 ],,,. [J ].,2003,3 (23) : 1872189. [14 ],,,. 9. 1C3 [J ].,1997,13 (3) :125. [15 ],,,. anti2 anti2id [J ].,2001,13 (4) :2042206. [16 ],,,. [J ].,1998, 14 :3782378. [17 ] Bailey N C,Fidanza V, Mayer R,et al. Activation of clones producing self2reactive antibodies by foreign antigen and antiidiotype antibody carrying the internal image of the antigen[j ]. Chin Invest,1989,84 (3) :7442756. [18 ] Samonigg H, Wilders2Truschnig M, Kass I,et al. A double2 blind randomized2phase II trial comparing immunization with antiidiotype goat antibody vaccine SCV 106 versus unspecific goat antibodies in patients with metastatic colorectal cancer [J ]. Immunother,1999,22 (6) :4812488. [19 ] Baral R N, Saha A, Chatterjee S K, et al. Immunostimulatory CpG oligonucleotides enhance the immune response of anti2idiotype vaccine that mimics carcinoembryonic antigen[j ]. Cancer Immunol Immunother, 2003,52 (5) :3172327. 59
[20 ] Reinartz S, Hombach A,Kohler S,et al. Interleukin26 fused to an anti2idiotype antibody in a vaccine increases the specific humoral immune response against CA125 + (MUC2 16) ovarian cancer [J ]. Cancer Research,2003,63 (12) : 323423240. [21 ],,,. NS3 [J ].,2001,15 (2) :1862188. [22 ] Ye Y,Yan L,Zhang X,et al. Influence of idiotypic nerwork regulation on renal transplant[j ].,1997,35 (9) :5272529. [23 ],,,.., 2002,15 (5) :3982401. [24 ],,,. [J ].,2000,16 (6) :4842485. [25 ],. [J ].,1996,23 (40) :1962200. [26 ],. [J ]., 2002,18 (6) :6782 681. [27 ] Cesco2Gaspere M, Benvenuti F, Burrone O R. BCL1 lymphoma protection induced by idiotype DNA vaccination is entirely dependent on anti2idiotypic antibodies [J ]. Cancer Immunol Immunother,2005,54 (4) :3512358. [28 ],,. [J ].,1996,22 (4) :2132215. [ :2005-08 - 02 ] :R15 ;Q933 :E :1004-8456 (2006) 01-0056 - 05 (2005) 109 : ([ 2005 ]18 ),: ( GB 19295 2003), 5 (),5 () 406, 300 000 cfuπg 87163 % ; 264, 10 000 cfuπg 98148 %,5 () 82, 150 cfuπg97156 % ; 40, 50 cfuπg 100 %,,,,,,,,, 60 2006 18 1