0 * EPS 2. 0004 2. 0044 MBR B-EPS NaOH + 6 B-EPS B-EPS B-EPS B-EPS NaOH + B-EPS B-EPS B-EPS DOI 0. 767 / j. issn. 000-8942. 203. 03. 003 EXTRACTION OF EXTRACELLULAR POLYMERIC SUBSTANCES FROM ACTIVATED SLUDGE IN MEMBRANE BIOREACTOR Li Jihong Shan Shiliang Li Liang Hu Xiaomin Dong Yihua 2. College of Resources and Civil EngineeringNortheastern UniversityShenyang 0004China 2. School of Biology and Environment EngineeringShenyang UniversityShenyang 0044China Abstract Taking the content of proteinpolysaccharide and nucleic acid as measurement index for bound extracellular polymeric substances B-EPS total quantitythe extraction of B-EPS from activated sludge in the membrane bioreactor MBR was studied by six methods including thermal treatmentultrasonicationnaoh + formaldehyde treatmentsulfuric acid treatmenthigh speed centrifugation and steam treatment. The optimal extraction conditions were determined by comparative analysis of proteinpolysaccharide and nucleic acid extraction amount. The results show that ultrasonicationhigh speed centrifugationsteam treatment and sulfuric acid treatment have a low performance on B-EPS extraction. NaOH + formaldehyde treatment is effectivebut causes measurement deviation and effects operation process. Thermal treatment is determined as the optimal extraction method with high extraction efficiency of B-EPS little cell disruption and simple process. Keywords membrane bioreactor extracellular polymeric substances extraction protein 0 membrane bioreactormbr EPS 3 EPS -3 4 4-9 MBR 70% ~ 80% 5 EPS 0-2 MBR * 578088 extracellular polymeric substances
EPS 2 EPS bound EPS B- EPS EPS soluble EPS S-EPS S-EPS B-EPS B-EPS EPS B-EPS 3 500 r / min 0 min NaOH 6-9 3 20 B-EPS B-EPS EPS 2 3 B-EPS NaOH + 6 MBR B-EPS. MBR 0. 2 m. 5 0. 08 m 0. 35 m 4. 5 L B-EPS PVDF 20 B-EPS Folin- Lowry 2 22 Longer pump YE55x 8 min 2 min 253Y 24 2 2 h ACO-38 LZB-6 EPS 0. m 3 / h ρ DO 4. 8 mg / L. 2. 3 5 ml 4. 4 EPS 5 000 mg / L 50 ml 2 3 4 5 6 7 8 9 0 2 3 2 B-EPS 23 2. B-EPS 2.. B-EPS 60 70 80 90 40 min 2 000 r / 5 min 0. 22 μm
2 3 3 B-EPS 5 ml 4 40 50 60 70 W 3 B-EPS 2 min 2 000 r / min 5 min 0. 22 μm 80 5 25 80 EPS B-EPS 80 2.. 2 B-EPS 5 ml 4 80 0 20 40 60 min 2 000 r / min 5 min 0. 22 μm 5 DNA 4 4 B-EPS B-EPS DNA 40 min B-EPS 80 40 min B-EPS 252. 4 mg / g 44. 66 mg / g 5. 45 mg / g 2. 2 B-EPS EPS 4 40 min EPS B-EPS 40 min 0. 5. 0 5 B-EPS B-EPS 50 W 2 min B-EPS 67. 34 mg / g 24. 63 mg / g DNA 5. 40 mg / g 2. 3 NaOH + B-EPS
3. 5 2. 0 mol / L NaOH 0 ml 30 ± 00 r / min 3 h 2 000 r / min ph B-EPS 5 min 0. 22 μm EPS EPS 6 29 NaOH B-EPS 2. 4 B-EPS 3 2 000 r / min 20 0% 20 min h. 5 h 6 NaOH B-EPS 2 6 NaOH. 5 mol / L 350. 67 35. 67. 5 mg / g NaOH B-EPS 67. 34 24. 63 5. 4 250. 67 40. 82 8. 28 ph NaOH 350. 67 35. 07. 5 26 27 NaOH + 65. 2 332. 5. 43 0 ml NaOH 66. 32 3. 56 3. 58 0. 0. 2 0. 3 0. 4 ml 2 B-EPS B-EPS 7 NaOH + > > 7 B-EPS 2. 5 B-EPS 2 B-EPS mg / g 96. 98 26. 05. 6 252. 24 44. 66 5. 45 > > > NaOH + > > > > > > > > > NaOH + B-EPS EPS EPS King Forster EPS 30 NaOH + 7 2% B-EPS 65. 2 332. 5. 43 mg / g NaOH Wingender 28 + NaOH B-EPS
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