Research Progress in Papaverine Determination

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17 5 2005 9 PROGRESS IN CHEMISTRY Vol. 17 No. 5 Sep., 2005 3 3 3 ( 100871),,,, : O65 ; R284. 1 : A : 10052281X(2005) 0520897208 Research Progress in Papaverine Determination Yan Jin Zhao Meiping 3 3 Li Yuanzong Chang Wenbao ( Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China) Abstract Being commonly used in clinical treatment and illegally used as additive in food products, papaverine and pericarpium papaveris have attracted many researchers to establish reliable determination methods. The structural characters and clinical use of papaverine and pericarpium papaveris are systematically summarized. Introduction and evaluation of related determination methods, including chemical analysis, spectral analysis, electrochemistry, chromatographic techniques, electrophoresis and immunoassay method are presented. The developing trend of papaverine determination is also proposed. Key words papaverine ; pericarpium papaveris ; determination (12[ (3,42 ) ]26,72 ), 1848,Merck [1 ] The structure of papaverine C 20 H 21 NO 4 Goldschmiedt [2 ] 1883, 1888 ( 1 %), ( ), Pictet Gams [1 ] 1909,, : 2004 9, : 2004 11 3 (No. 2001BA804A21) 3 3 e2mail : mpzhao @pku. edu. cn

898 17,, 011 molπl [3, ], [ 4], 2., 5 %,, 5 %,,, 100 500mgΠkg Papaver somniferum L.,, 1. [4, ],,, Abdel2Ghani [6 ], chromotrope 2B, (C2B) chromotrope 2R(C2R), 4 (C2B, 536 524nm, C2R 540 528nm), ( ) 9918 % ( ) 10011 %,, El2Zeany [7 ] 54 %, 90 %,, 015 2h, 24h 310nm, 287nm 42, Vierordt, 100 % [8 ] 2 %, : [ 5] 1.,, 92 %,, 87 %, Mirzaeva [9 ] Dimedrol ( ), Sulfonazo,, (5 3)

5 899 580nm (CL) Dimedrol, 9615 % 9715 % Zhuang [13 ] 2., : [10, ] - - HSO 3 + MnO 4 2-2 HSO 3 S 2 O 6 + 2H +, 2-2- 3 2 Tris2HCl S 2 O 6 SO 4 + SO 2 252nm 3 SO 2 SO 2 + h, 500nm, 012 10 molπl, ; 2 110 molπl (3S),RSD 210 %, 2,,, OH, 2,, Zhang [14 ], ( ), 2, 2 ( ),, 1106 10-7 3118 10-6 molπl, 1210ngΠmL [11, ], (BSA),, ( ) BSA, 011 10 molπl, BSA 019991,RSD 210 %, 87nmolΠL (3, ) Baudot [12 ] 3 4., Eisman [15 ] (AAS) 3. 2- HSO 3 + MnO 4 ( ) 360nm, ( ) Reinecke AAS 150 h - 1,, 5 85mgΠmL 50, 850mgΠmL,RSD 113 % 312 %,,

900 17,,, [16 ], NH 3 2NH 4 Cl TLC,,, Rajananda [20 ] TLC, 1975 1985 10 Frumkin, 2 14 38 0186 : TLC, TLC Szabo [22 ] TLC,, 810 10-9 HPTLC, 6 110 10-7 molπl, 110 10-9 molπl 2 2 2 Yegorov [17 ] PVC 25 % (20 20 3 1,vΠv) ; 2 2 2 (20 20 3 1,vΠv) [18 ; Dragendorff ph ] Marquis 600nm 520nm [23 ] 2 (9 1,vΠv) 2182 10-3 1100 10-6 molπl, G, 2 3130 10-7 molπl 2, [19, 9910 % 10110 % ], 4 gπl ( 21 015g ΠL ) A 1 B 1 PVC TLC HPTLC 1100 10-2 1100 ( 15 10-5 molπl, 1199 10-6 molπl ),, 98 % 103 %,RSD 2. ( GC) 314 % GC, GC, GC 1. ( TLC) ( HPTLC) TLC HPTLC [21 ] GS2910 GF 254, 2 (4 1) 5, 01007 %, 01004 %, (FID)

5 901 [24 ] 115 %OV217, 2166 10-4 7198 10-4 molπl (011 013mgΠmL, R, FID = 019972), 50ng (3 SΠN) 01444, 1111 gπml Kikura2Hanajiri [39 ] (APCI2MS) 01010 % 01033 % [25 ] 0153mm ( HP21 M3G ), FID, ODS23,, 3 8 ( ) 0101 0104 g 4 Bakkali [32 ] 160 gπml, 0150 gπml 72h 3 (SPE), C 18 [26 ] GC2FID ( 212nm) 4 4, ( ) 80 % Doner [36 ],, HP25,, HPLC 201025molΠL KH 2 PO 4 (75 25, vπ v) 286nm Paul [27 ] GC, 16min, 014ngΠmL, HPLC, 67 gπg, 0117 gπg, GC 3. ( HPLC) HPLC TLC HPTLC HPLC HPLC, 10,HPLC, HPLC, GC, HPLC [28 ] [29 34 ] [35, 36 ] [37, 38 ] 4., (CE) 20 80 HPLC,, [30 ] C 18, CH 3 OH225mmolΠL KH 2 PO 4 (90 10,vΠv), (254nm, ), (CZE) (MEKC), 3, 011 gπml Borojevic [33 ] [40 C 18 2 ] MEKC, 62 8 ;

902 17, 10mmol 25mmol SDS 10 % (vπv) (ph 9115 9140),8, 018 115mgΠL Bjgrnsdottir [41 ] CE, DMF DMSO, (25mmolΠL)2 (1molΠL) [45, 46, 25 % 2 ] [47 ], 25, [48 ], (DAD), 214nm, [42 ] Yeremenko [49 ] 1992 MEKC, 5, ( ELISA) 62 (0105molΠL,pH = 410), 32 2, ( 220 SDS) 5 (BSA) ELISA (CD) : (25mmolΠL 220 12mmolΠL SDS) ( HRP) ;,,, 1 gπml ; [50 ] CD,, Yeremenko 30mmolΠL DM2 2CD, ELISA Reddy [43 ] CZE 5, ; ph 3,,, 215 % 3 ( = 3), 450ngΠmL ( ) 500ngΠmL ( ) 550ngΠmL,6,72 ( ) 500ngΠmL ( ) RSD RSD 1103 % 3156 % 0134 % 0169 %, 0192 % [51 2137 % ] [44 ] HPCE, BSA 3, : 50mmolΠmL 6,72 (ph = 710) ;,9min 3 1 (10 5 10 6 ), ;, 01033 % ;, 1 % HPLC,CE 2

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