41 2 ( ) Vol.41 No.2 2010 4 Journal of Central South University (Science and Technology) Apr. 2010 1 2 2 2 (1. 330013 2. 410083), 79 70 74 85.81% 87.35% 86.76% 44 53 / O657 A 1672 7207(2010)02 0440 06 Comparison analysis of common volatile constituents in herbal pair radix saposhnikoviae-rhizoma seu radix notopterygii and its single herbs CHEN Shuai-hua 1, YU Lian-fang 2, LI Xiao-ru 2, LIANG Yi-zeng 2 (1. School of Chemistry and Chemical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330013, China; 2. School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China) Abstract: Comparison analysis of common volatile constituents in herbal pair (HP) radix saposhnikoviae (RS)-rhizoma seu radix notopterygii (RSRN) and its single herbs was performed using two-dimensional gas chromatography-mass spectrometry (GC-MS) data coupled with alternative moving window factor analysis (AMWFA). In total, 79, 70 and 74 volatile chemical components in volatile oil of RS, RSRN and HP RS-RSRN were separately determined qualitatively and quantitatively, accounting for 85.81%, 87.35% and 86.76% of total contents of volatile oil in RS, RSRN and HP RS-RSRN respectively. The results show that there are 44 common volatile constituents in HP RS-RSRN and single herb RS, and 53 common volatile constituents in HP RS-RSRN and single herb RSRN. The experimental results also show that the volatile chemical components in HP RS-RSRN are mainly from single herb RSRN. Key words: radix saposhnikoviae; rhizoma seu radix notopterygii; herbal pair; gas chromatography-mass spectrometry; alternative moving window factor analysis [1] (RS) (RSRN) 2009 01 28 2009 04 14 (2017503 20235020) (1963 ) 0731-88836376 E-mail: xrli@mail.csu.edu.cn
2 441 [2 3] GC-MS (AMWFA) [4 7] [8 10] AMWFA 2 2 [4 7] 1 1.1 QP2010 C 8 ~C 20 ( Fluka Chemika ) 1.2 100 g [11] 100 g 1.3 OV-1( 30 m 0.25 mm) 50 2 /min 130 10 /min 230 20 min He 1.0 ml/min 250 280 EI 70 ev 230 1.28 kv 20~600 3.8 /s 2 min 1.4 Matlab 6.1 NIST107 2 2.1 AMWFA 1 GC-MS (TIC) TIC 13.331~13.998 min X TIC 13.498~14.162 min Y 2 X Y 2 X Y 2 AMWFA 3 2 AMWFA 1 X Y ( Y ) ( 3) 4(a) 2 4(b) 3 1 (13~38 88~111 132~144) 3 1 3 NIST107 1 2 3 Furan 2-pentyl- Octanal beta-myrcene 99.47% 94.83% 95.21% X Y 5 X Y 1 2.2 Vanden [12] R I =100n+100[(t x t n )/(t n+1 t n )] t x n n+1
442 ( ) 41 (a) (b) (c) 1 Fig.1 Total ion chromatograms patterns of volatile oils of RS, RSRN and HP RS-RSRN (a) X (b) Y 2 X Y Fig.2 Total ion chromatograms for X peak cluster and Y peak cluster
2 443 3 Fig.3 Results of common rank analysis 1 2-pentylfuran; 2 octanal; 3 β-myrcene (a) X (b) Y 5 X Y Fig.5 Resolved chromatograms for peak clusters X and Y (a) (b) 4 Fig.4 Resolution results of common components [13 14] 2.3 79 70 74 ( ) 85.81% 87.35% 86.76% 1 2.4 1 (14.85%) (10.78%) D- (5.53%) (4.98%) (S)-1- -4-(5- -1- -4- )- (4.86%) α- (20.93%) D- (9.57%) α- (8.05%) 4- -1-( )-3- -1- (5.74%) γ- (4.83%) (3.28%) - α- (22.32%) α- (8.18%) D- (7.71%) 4- -1-( )-3- -1- (5.98%) (3.38%) (2.36%) (1.89%) 2 44 53 ( ) ( ) [15 16]
444 ( ) 41 1 Table 1 Main chemical components of volatile oils from RS, RSRN and HP RS-RSRN r c /% RS RSRN RS-RSRN R I 1 C 7 H 14 O 1.17 0.41 865.394 2 α- C 10 H 16 3.42 20.93 22.32 925.164 3 C 10 H 16 0.32 3.28 3.38 931.962 4 C 8 H 16 O 4.98 0.27 1.89 974.162 5 D- C 10 H 16 5.53 9.57 7.71 1014.58 6 γ- C 10 H 16 1.79 4.83 1.73 1039.94 7 4- -1-( )-3- -1- C 10 H 18 O 0.99 5.74 5.98 1151.77 8 α- C 10 H 18 O 8.05 8.18 1170.17 9 C 10 H 8 O 2 14.85 0.77 1.75 1219.49 10 C 12 H 20 O 2 0.29 2.35 2.36 1257.15 11 (S)-1- -4-(5- -1- -4- )- C 15 H 24 4.86 0.35 1485.25 12 C 17 H 24 O 10.78 1.78 1996.98 : r c R I 3 (1) 79 70 74 85.81% 87.35% 86.76% (2) 44 53 (3) 2 [1],,. [M]. :, 1996: 363. XU Qing-hua, LIU Li-hua, ZHAO Rui-hua. Collection of drug pairs in traditional Chinese medicine[m]. Beijing: China Press of Traditional Chinese Medicine, 1996: 363. [2],. [M]. :, 2004: 52 54. TAN Tong-lai, LIU Qing-ling. Common-used herbal pairs and contraindictions of traditional Chinese medicine[m]. Taiyuan: Shanxi Press of Science and Technology, 2004: 52 54. [3]. [M]. :, 2000: 984 985, 1254 1256. TIAN Dai-hua. Practical dictionary of traditional Chinese herbs[m]. Beijing: People s Medical Press, 2000: 984 985, 1254 1256. [4] ZENG Zhong-da, LIANG Yi-zeng, WANG Ya-li, et al. Alternative moving window factor analysis for comparison analysis between complex chromatographic data[j]. J Chromatogr A, 2006, 1107: 273 285. [5],,,. [J]., 2006, 27(11): 2052 2055. YI Lun-zhao, LIANG Yi-zeng, ZENG Zhong-da, et al. AMWFA method applied to comparative analysis of two-dimensional data with overlapped peaks[j]. Chem J Chin Univ, 2006, 27(11): 2052 2055. [6],,,. GC-MS (AMWFA) 3 [J]., 2006, 27(9): 1626 1630. YI Lun-zhao, LIANG Yi-zeng, ZENG Zhong-da, et al. Comparative analysis of volatile constituents in Chenpi of different original plants by GC-MS and AMWFA[J]. Chem J Chin Univ, 2006, 27(9): 1626 1630. [7],,,. -
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