Chinese Journal of Zoology 2009,44 (3) :63 69 3 ( 710061 ; 710400 ; 710032) :2007 11 12 18 75 Π, 11, 8819 %, ( Blastocystis sp. ) : ; ; :Q954 :A :025023263 (2009) 03263207 Observation on Intestinal Parasitic Morphology and Infection in Captive Rare Wildlife in Shaanxi Province CAI Juan QIAO Ji2Ying 3 ZHANG Xu J IN Xue2Lin REN Jian2She MA Qing2Yi WU Xiao2Min ( College of Medicine, Xi an Jiaotong University, Xi an 710061 ; Shaanxi Rescuing & Raising Research Centre for Rare Wild Animals, Xi an 710400 ; Shaanxi Institute of Zoology, Xi an 710032, China) Abstract: Intestinal parasitic infection of rare wildlife in the protection institution, Shaanxi Province, China was investigated from November to December,20071 Feces of 75 individuals of 18 species were examined by using wet mount smear and iodine staining. Digital micro2photograph was taken when positive specimens were detected. Eleven species of parasites were detected in this study and the infection rate in the examinate samples was as high as 8819 %, Blastocystis sp. and amebic protozoa were the most common parasites to infect the captive individuals of wildlife in the protection institution. Key words :Rare wildlife ; Intestinal parasites ; Morphology,,,,,,, (No. 2006BAD03A2 1202), (2007ZD201) ; 3,E2mail :qiaojiying2000 @sina. com. cn ;,, ; : ; E2mail :xjbolecj @sohu. com :2008208219, :2008212231
64 Chinese Journal of Zoology 44 [1 4 ] ( Budorcas taxicolor) 16 [5 ] 2007 11 12 1 111 ( Cygnus cygnus) ( Pavo muticus ) ( Crossoptilon mantchuricum) ( Budorcas taxicolor) ( Capricornis sumatraensis ) ( Naemorhedus goral ) ( Ursus thibetanus ) ( Ailuiopodidae melanoleuca ) ( Ailurus f ulgens) ( Tragopan temminckii) ( Chrysolophus pictus) ( Sus scrof a) ( Rhinopithecus roxellanae) ( Macaca mulatta ) ( Moschus berezovskii ) ( Muntiacus reevesi ) ( Cervus nippon temminck) ( Panthera pardus) 18 75 Π 112, 80 %, 4 113, 3 2 211 2007 11 12 18 75 Π, 11, 2 ; 1 ; 1 ; 7 8819 %, 1 3 6616 %, 3 4 %, 1 212 11 ( 2) 213 21311 (1) :,, 80 m 60 m,,,, ( : 1) (2) :,, 90 m 40 m,,, ( :2) (3) :,, 75 m 50 m,,,, ( :3) (4) :,, 60 m 24 m,, ( :4 5) (5) :,, (38 45) m (22 30) m,, ( :6) (6) :, 25 40 m,,,,, ( : 7) 21312 (1) :,,,,, 12 m( :8) (2) : 22 m,,,, ( :9)
3 : 65 Host species 1 18 Table 1 Parasitic infection in the samples of 18 wildlife species Infected Infection rate Samples samples ( %) Parasites Cygnus cygnus 2 2 100 Blastocystis sp. ++++ Pavo muticus 9 3 3313 B. sp. + Infection intensity 3 3313 Endolimax nana + 2 2212 Cestode + 2 2212 Capillaria sp. + 2 2212 Ascaridia galli + 1 1111 Coccidia + Crossoptilon mantchuricum 2 2 100 Blastocystis sp. + Budorcas taxicolor 3 2 6617 Entamoeba coli ++ Capricornis sumatraensis 1 1 100 E. coli + 1 100 E. histolytica + Naemorhedus goral 11 2 1812 Blastocystis sp. ++ 3 2713 E. histolytica ++ 1 911 Trematode + Ursus thibetanus 2 1 50 E. histolytica + Ailuiopodidae melanoleuca 2 0 0 Ailurus fulgens 2 0 0 Tragopan emminckii 2 1 50 B. sp. +++ 1 50 E. coli + Chrysolophus pictus 11 10 9019 B. sp. + 1 911 A. galli + Sus scrofa 5 1 20 B. sp. + 1 20 Endolimax nana + 3 60 Ascaris suum ++ Rhinopithecus roxellanae 12 8 6617 B. sp. ++ 8 6617 Entamoeba histolytica + 3 25 E. coli + 10 8313 Capillaria sp. + Macaca mulatta 1 1 100 E. histolytica + Moschus berezovskii 2 1 50 Cryptosporidium + Muntiacus reevesi 2 2 100 B. sp. + 1 50 Cestode + Cervusnippon temminck 3 1 3313 B. sp. + Panthera pardus 3 0 0 2 6617 Cryptosporidium + 1 3313 Giardia lamblia + ; + 1 3 ; ++ 4 6 ; +++ 7 represents no egg detected ; + 1-3 eggs observed in each field of vision ; ++ 4-6 eggs in each field of vision ; +++ 7 + eggs in each field of vision.
66 Chinese Journal of Zoology 44 2 Table 2 Hosts infected by parasites Parasitic species ( %) Infection rate Infected wild animals distribution Blastocystis sp. 4617 Entamoeba histolytica 1817 E. coli 913 Endolimax nana 513 Giarda lamblia 113 Cryptosporidium 410 Coccidia 113 Ascaridia galliπascaris sum 810 Capillaria sp. 1610 Cestode 410 Trematode 113 (3) :,,,, 11 m( :10 11) (4) :,, 215 813 m( : 12) (5) :, 2717 m 1712 m,,,,,, ( :13) 3 311 11,,, [6 ],,, ( Canis f amiliaris) ( Felis catus) ( Sus domestica) ( Mus musculus) ( Oryctolagus cuniculus) ( Camelus ferus) ( Panthera leo) [7 ],, [8 ], [1 ],, (7 ) Gomez 17 34 183, 6, [9 ],,,, 312
3 : 67,,,,,,, ;,, [10 ],,, [ 1 ],,..,2007,34 (10) :104 105. [ 2 ],,..,2007,26 (2) :451 453. [ 3 ],,..,2001, (1) :25 26. [ 4 ],,..,2002, 10 (3) : 63 64. [ 5 ]..,1994, (6) : 38 39. [ 6 ],,. RPMI1640.,2006, 6 (2) : 161 164. [ 7 ]..,2002,8 (3) :104 107. [ 8 ],,..,2007,15(5) :45 48. [ 9 ] Gomez M S, Vila T, Feliu C, et al. A survey for Cryptosporidium spp. in mammals at the Barcelona Zoo. Int J Parasitol,1996,26(1) :1 332 1 333. [10 ],..,2006, (16) :26. 11, 100 ; 21, 400 ; 31, 400 ; 41, 400 ; 51, 400 ; 61, 400 ; 7., 400 = 1 m Explanation of Plate 11 Fertilized egg of Ascaris suum in Sus scrofa,wet mount smear 100 ; 21 Unfertilized egg of A. suum in S. scrofa,iodine staining 400 ; 31 Egg of A. galli in Pavo muticus,wet mount smear 400 ; 41 Egg of Capillaria sp. in P. muticus,wet mount smear 400 ; 51 Egg of Capillaria sp. in Rhinopithecus roxellanae,iodine staining 400 ; 61 Egg of D. dendriticum in Naemorhedus goral,iodine staining 400 ; 71 Cestode egg in P. muticus,wet mount smear 400. Bar = 1 m 81, 400 ; 91, 400 ; 101, 400 ; 111 ( ), 400 ; 121, 400 ; 131, 400 = 1 m Explanation of Plate 81 Trophozoite of Entamoeba coli in Rhinopithecus roxellanae,wet mount smear 400 ; 91 Cyst of E. coli in R. roxellanae (arrow),iodine staining 400 ;101 Cyst of E. histolytica in Muntiacus reevesi,iodine staining 400 ;111 Cyst of E. histolytica in R. roxellanae,iodine staining 400 ;121 Blastocystis sp. in Crossoptilon mantchuricum,iodine staining 400 ;131 Eimeria tenella in Pavo muticus,wet mount smear 400. Bar = 1 m
68 Chinese Journal of Zoology 44 : CAI J uan et al. :Observation on Intestinal Parasitic Morphology and Infection in Captive Rare Wildlife in Shaanxi Province Plate
3 : 69 : CAI J uan et al. :Observation on Intestinal Parasitic Morphology and Infectionin Raised Rare Wildlife in Shaanxi Province Plate