(Ch inese J R ice S ci) 2001 15 (1) : 35 40 Rub isco Rub isco ( 310029) R ub isco A ctivase and Its R egu lation on D iu rnal Changes of Pho to syn thetic R ate and the A ctivity of R ibu lo se 1 52B ispho sphate CarboxyasegO xygenase (R ub isco) W EN G X iao2yan LU Q ing J IAN G D e2an (D ep artm ent of B iology Z hej iang U niversity H ang z hou 310029 Ch ina) Abstract: To clarify the facto rs determ ining the diurnal courses of pho to synthesis in a day pho to synthetic rate (P n) pho2 to synthetic pho ton flux (PPF ) stom atal conductance (S c) intercellular CO 2 concentration (C i) relative hum idity (RH ) tem 2 perature (T ) transp iration (T rans) initial Rubisco activity (R I) to tal Rubisco activity (RT ) Rubisco activase activity (RCA ) w ere m easured. The m axim al pho to synthetic rate occurred at about 8: 30 a. m. w hen initial Rubisco activity reached the m axi2 m um. Then bo th of them gradually decreased dow n. A fter 13: 00 p. m. they ro se up po sitively co rrelated w ith P n and the re2 gression coefficient betw een them w as 0. 9368. By the co rrelation analysis and path analysis stom atal conductance and initial Rubisco activity w ere found to be m ajo r facto rs lim iting pho to synthetic rate. The interaction of Rubisco activase activity and o ther facto rs on pho to synthetic rate w ere studied by using response surface and the response surface of every m athem atical model w as draw n to exp ress their intrinsic relationship. The study show ed that Rubisco activase activity had an effect on pho2 to synthetic rate by its regulating initial Rubisco activity. Key words: pho to synthesis; enzym e activity; environm ental facto r; co rrelationship; facto r analysis; rice : Rubisco Rubisco CO 2 Rubisco Rubisco Rubisco Rubisco Rubisco : ; ; ; ; ; : Q 945; S311 : A : 100127216 (2001) 0120035206 R ubisco R ubisco R ubisco 1 2 R ubisco 3 4 R ubisco 1996 R ubisco R ubisco 5 R ubisco R ubisco R ubisco R ubisco R ubisco R ubisco R ubisco 1 6 R ubisco R ubisco 1. 1 7 9 R ubisco 11 ph A T P 1992 L an 10 R ubisco R ubisco R ubisco : 1999212206; : 2000204203 : (39970440) : (1966- )
36 15 1 (2001 1 ) 1. 2 R ubisco [RCA CO 2 Λmo lg(m 2 s) ] L I26000 ( ) 1 L 2 2 8: 00 18: 00 14 1. 3 Rubisco Rubisco CO 2 (P n) 8: 30 14 C 13: 00 12 15: 00 (PPF ) 11: 00 14: 00 0. 1 13: 00 g 12 000 g 10 m in R ubisco R ubisco 2 (S c) 8: 30 11: 00 [ CO 2 Λmo lg(g s) = dpm V 10 1. 5g(Ν t V 60) : dpm dpm - dpm V (C i) 12: 00 (ΛL ) Ν 1 mo l N ah 14 CO 3 dpm t (s) V (ΛL ) R ubisco (RH ) 13: 00 14: 00 ; R ubisco 60% 36% 1. 4 Rubisco [ P n R ubisco R ubisco CO 2 Λmo lg(m 2 s) ] 4 R ubisco : x 1- [ PPF Λmo l g(m 2 s) ; x 2- R ubisco R ubisco (RH % ) ; x 3- [S c H 2O mo lg(m 2 s) ; x 4- [T rans H 2O mo lg(m 2 s) ; x 5 - CO 2 (C i ΛL gl ) ; x 6- (T ) ; x 7 - R ubisco [R I CO 2 Λmo lg(m 2 s) ; x 8-2. 1 ( 1) 12: 00 13: 15: 00 CO 2 3 (T ) 2. 2 R ubisco 12: 00 15: 00 ; R ubisco R ubisco 1 F ig. 1. The diurnal change of pho to synthetic rate (P n) and pho to synthetic pho ton flux (PPF).
: Rubisco Rubisco 37 2 CO 2 F ig. 2. The diurnal change of intercellular CO 2 concentration (C i) and stom atal conductance (S c). 3 F ig. 3. The diurnal change of temperature and relative hum idity. (P n) ( 1) R ubisco (R I) (S c) (P n) 0. 9368 0. 7300 ; R ubisco (RCA ) (T rans) (P n) 0. 6466 0. 6685 2. 4 Py x i : 4 Rubisco Rubisco Py x 7 = 018125 Py x 5 = - 015581 Py x 3 = 014747 Py x 1 = - 014009 Py x 4 = - 011786 Py x 6 F ig. 4. The diurnal change of Rubisco activity and Rubisco acti2 = - 010205 Py x 2= - 010195 Py x 8= 010090 vase activity. Ηy x i 8: 30 Ηy x i= 2Py x irij j 1 i = 1 2 8; 14: 00 1 Py x irij x i x j : (P n) R ubisco Ηy x 3 = 018471 Ηy x 1 = 017844 Ηy x 8 = 016376 R ubisco Ηy x 5= 013331 Ηy x 4 = 012554 Ηy x 2= 012161 Ηy x 6 = - 012053 Ηy x 7= 011242 2. 3
38 15 1 (2001 1 ) 1 (x i) (y ) Table 1. The correlation analysis between photosynthetic rate and factors and rank of general effects of factors on photosynthetic rate. Facto r CO 2 R ubisco R ubisco PPF (x 1) RH (x 2) S c (x 3) T rans (x 4) Coefficient 0. 3836 0. 1966 0. 7300 3 3 0. 6685 3-0. 2251-0. 2258 0. 9368 3 3 0. 6466 3 Rank of general effects D irect 4 7 3 5 2 6 1 8 Indirect 2 6 1 5 4 7 8 3 3 3 3 0. 05 0. 01 3 3 3 Significant at 0. 05 and 0. 01 levels respectively. The sam e as in the tables below. C i (x 5) T (x 6) R I (x 7) RCA (x 8) 2. 5 Rubisco 2 Rubisco ( 1) 1 x 3 ( ) x 7 (R ubis2 co ) R ubisco 5 R u2 Po rtis 13 R ubisco bisco ; R ubisco R ubisco R ubisco R ubisco R ubisco R ubisco ( 2) y 18 = - 313838 + 010369x 1-014250x 8 + 016003x 2 8-010036x 1x 8 y 78 = 515880-010829x 7 + 015904x 8 + 010133x 2 7-010858x 2 8+ 010078x 7x 8 y 58 = - 7617224 + 018610x 5-1619915x 8-010019x 2 5 + 013026x 2 8+ 010569x 5x 8 y 28 = - 1919466 + 212516x 2-1117389x 8-010347x 2 2 + 011306x 2 8+ 012590x 2x 8 y 68= - 23311326+ 1313985x 6 + 916740x 8-011845x 2 6 + 011061x 2 8-012614x 6x 8 y 18 y 78 y 58 0. 01 y 28 y 68 0. 05 Table 2. Correlation between Rubisco activase activity and factorṡ R ubisco 6 R ubisco 35 ; 35 2. 5. 1 R ubisco ; R ubisco R ubisco R ubisco 30 Facto r Coefficient Significance ( P ) PPF (x 1) 0. 9508 3 3 0. 0001 R ubisco R I(x 7) 0. 9400 3 3 0. 0002 CO 2 C i (x 5) 0. 9333 3 3 0. 0003 RH (x 2) 0. 8754 3 0. 0190 T (x 6) 0. 8400 3 0. 0300 R ubisco R ubisco R ubisco R ubisco ; R ubisco R u2 bisco R ubisco 2. 5. 2 R ubisco
: Rubisco Rubisco 39 5 Rubisco Rubisco 6 Rubisco F ig. 6. Relationship among the pho to synthetic rate Rubisco ac2 F ig. 5. Relationship among the pho to synthetic rate Rubisco ac2 tivase activity and intitial Rubisco activity. tivase activity and temperature. R ubisco 2. 5. 4 R ubisco 2. 5. 3 R ubisco CO 2 CO 2 ( 7) R ubisco R ubisco 8 CO 2 R ubisco ; R ubisco R ubisco ; CO 2 CO 2 R ubisco R ubisco CO 2 R ubis2 co CO 2 R ubisco 2. 5. 5 R ubisco ; CO 2 CO 2 R ubisco 7 Rubisco CO 2 F ig. 7. Relationship among the pho to synthetic rate Rubisco ac2 tivase activity and intercellular CO 2 concentration. 8 Rubisco F ig. 8. Relationship among the pho to synthetic rate Rubisco ac2 tivase activity and relative hum idity.
40 15 1 (2001 1 ) and canopy pho to synthetic rates [J. P lant P hy siol 1987 83: 9 Rubisco 573 578 F ig. 9. Relationship among the pho to synthetic rate Rubisco ac2 tivase activity and pho to synthetic pho ton flux. 9 R ubis2 co 5. R ubpcase ; R ubisco [J. 1996 32 (4) : 257 260 ; R ubisco 6. R ubisco 1999 25 (3) : 263 266 R ubisco ; R ubisco 3 1700 Λmo lg(m 2 s) 38 60% 36% 12. (R ubp) 14 [J. 1986 12: 33 39 2 15 R ubisco R ubisco 14: 00 14. [J. Po rtis R ubisco 1998 12 (2) : 105 108 R ubisco R ubisco R ubisco R ubisco ph A T P 13 a facto r[j. ph A T P 17 R ubisco R ubisco : 1. CO 2 [J. 1992 18 (1) : 1 7 2 V u J C V A llon L H Gow es G. D rought stress and elevated CO 2 effects on soybean ribulo se bispho sphate carboxylase activity 3. [J. 1990 26 (6) : 5 10 4 Tenhum en J D L ange O L Gebel J et al. Change in pho to syn2 thesis capacity carboxylation efficiency and CO 2 compensation po int associated w ith m idday stom atal clo sure and m idday dep res2 sion of net CO 2 exchange of leaves Q uercussuber [J. P lanta 1984 162: 193 203 [J. 7 Feller U C rafts2b randner S J Salvucci M E. M oderately high temperatures inhibit ribulo se21 52bispho sphate carboxylasegoxy2 genase activasegm ediated activation of Rubisco [ J. P lant P hy siol 1998 116: 539 546 8 L illey R M Po rtis A R J r. A TP hydro lysis activity and po lym er2 ization state of ribulo se21 52bispho sphate carboxylasegoxygenase activase[j. P lant P hy siol 1997 114: 605 613 9 Po rtis A R J r. The regulation of R ubisco by R ubisco activase[j. J E x p B ot. 1995 46: 1285 1291 10 L an V W oodrow I E M o tt K A. L ight2dependent changes in ribulo se bispho sphate carboxylase activase activity in leaves [J. P lant P hy siol 1992 99: 304 309 11. [J. 1987 13 (4) : 441 444 13 Po rtis A R J r. Regulation of ribulo se21 52bispho sphate car2 boxyllasegoxygenase activity [J. B iol 1992 43: 415 437 A nn R ev P lant P hy siol M ol 15 Farquhar G D Sharkey T D. Stom atal conductance and pho to2 synthesis [J. A nn R ev P lant P hy siol 1982 33: 317 345 16 Streusand V I Po rtis M. Rubisco activase m ediates A TP2depen2 dent activation of ribulo se bispho sphate carboxylase [ J. P hy siol 1987 85: 152 154 P lant 17 Berkow itz G A Gibbs M. R educed o smo tic po tential effects on pho to synthesis identification of strom al actification as m ediation P lant P hy siol 1983 71: 905 911