Supplementary Figure 1

NSC N2d N5d TNFRSFa TNFRSFb RIPK RelA Madd I-TRAF/TANK GAPDH γ-actin Supplementary Figure

A 2 hrs Day : Cell seeding Day 9: +ng/ml TNF-α Day 9: Fixation B Merge Nestin PH-3 Hoechst Acute TNF-α Control C % positive cells 9 8 7 6 5 4 3 2 Control Nestin TNF-α acute D % positive cells 3 25 2 5 5 Control * PH3 TNF-α acute Supplementary Figure 2

35 Apoptosis in NSC/Diff 3 % double-positive cells 25 2 5 5 Nestin / c-casp 3 TuJ / c-casp 3 GFAP / c-casp 3 Control Chronic TNF-α Supplementary Figure 3

Merge Hoechst EdU Dcx Cell Profiler Output Cell Profiler Input Supplementary Figure 4

A Day : Surgery 6-OHDA or sham Day 5: Start daily IP Minocycline or NaCl Day 2: 2x EdU IP Day 24: Perfusion LV RMS Striatum SN B Hoechst TH TH Ipsilateral Contralateral IF IF Supplementary Figure 5

5 Sham Sham + Minocycline 2 4 35 3 25 2 5 5 m] m] 5 5 5 5 Distance to LV [µ Distance to LV [µ 5 27 253 379 55 63 757 883 9 35 7 33 49 65 8 97 3 29 45 8 6 24 32 4 48 56 64 72 8 235 352 469 586 73 82 937 54 2 5 Distance to LV 55 5 45 4 35 3 25 2 5 5 4 35 3 25 4 35 3 25 Distance to LV Disatnce to LV 2 5 5 5 2 5 5 8 235 352 469 586 73 82 937 54 87 73 259 345 43 57 63 689 775 2 23 34 45 56 67 78 89 9 2 42 63 84 5 26 47 68 89 4 55 5 3 35 5 45 45 3 4 25 4 35 25 35 2 3 2 3 25 25 5 Distance to LV Distance to LV to LV to LV 2 5 5 82 63 2444 325 46 487 568 649 73 Distance 5 5 5 64 327 49 653 86 979 42 35 468 85 69 253 337 42 555 589 673 7577 Distance 2 5 5 356 7 66 42 776 23 2486 284 396 Supplementary Figure 6a

6 OHDA 6 OHDA+ Minocycline 25 2 ] 5 5 5 Distance to LV [µm 2 5 5 7 33 49 65 8 97 3 29 45 6 9 27 325 433 54 649 757 865 973 8 24 47 7 93 6 39 62 85 28 23 # Cell # Cell 3 25 2 Distance to LV 45 4 35 3 25 2 5 5 5 5 43 285 427 569 7 853 995 37 279 42 43 285 427 569 7 853 995 37 279 42 5 45 4 35 3 25 2 35 3 25 2 5 # Cell Disatnce to LV to LV [mm] 5 97 93 289 385 48 577 673 769 865 96 Distnce 5 5 53 35 457 69 76 93 65 27 369 52 Supplementary Figure 6b

A Day : Surgery 6-OHDA Day 5: Start daily IP NS-398 or control Day 2: 2x EdU IP Day 24: Perfusion B merge 6-OHDA Hoechst EdU 6-OHDA + NS-398 C Fold increase EdU+ / total cells (6-OHDA=).6.4.2.8.6.4 *.2 6-OHDA 6-OHDA + NS-398 EdU e D 6-OHDA Hoechst Dcx E Migration: Dcx+ cells per distance category (6-OHDA = ) 6 5 4 3 2 * 6-OHDA + NS-398-5μm 6-OHDA >5μm 6-OHDA + NS-398 Supplementary Figure 7

6 OHDA 35 3 4 35 3 Distance to LV [µ µm] 25 2 5 5 25 2 5 5 Distance to LV 5 5 2 5 5 87 73 259 345 43 57 63 689 775 86 3 6 9 2 5 8 2 24 27 3 Dista nce to LV 5 29 33 47 52 625 729 833 937 Dista 85 69 253 337 42 55 589 673 757 nce to LV 45 4 35 3 25 2 3 25 2 35 3 25 2 5 2 LV Distance to LV stance to LV 5 5 5 5 5 5 5 22 423 634 845 56 267 478 689 9 2 2 Distance to 93 85 277 369 46 553 645 737 829 92 2 3 4 5 6 7 8 9 4 27 3 43 56 69 722 825 928 3 Di 88 75 262 349 436 523 6 697 784 87 2 3 4 5 6 6 7 8 25 2 2 5 5 5 5 2 5 5 86 7 256 34 426 5 596 68 766 85 Distance to LV [µ µm] 47 293 439 5855 73 8777 23 69 35 46 8 359 538 777 896 75 254 4333 62 79 Supplementary Figure 8a

45 4 35 3 25 2 5 6 OHDA + NS398 5 2 5 5 2 5 5 25 2 5 5 Distance to LV [ µm] 76 5 226 3 376 45 526 6 676 75 Distance to LV Distance to LV Distance to LV 4 27 3 43 56 69 722 825 928 3 26 5 76 26 5 76 2 226 25 52 33 454 65 756 97 58 29 36 5 5 55 5 45 4 35 3 25 2 45 45 5 4 35 4 3 35 25 3 2 25 2 5 5 Distance to LV 5 5 5 5 5 29 257 385 53 64 769 897 25 53 27 43 69 825 3 237 443 649 855 26 22 43 64 85 6 27 48 69 8 2 235 469 73 937 7 45 639 873 27 234 4 35 3 4 35 3 Distance to LV 25 2 5 5 2 5 5 25 2 5 5 Distance to LV Distance to LV 65 29 93 257 32 385 449 53 577 64 88 375 562 749 936 23 3 497 684 87 26 25 376 5 626 75 876 26 25 Supplementary Figure 8b

A Day : Surgery 6-OHDA or sham Day 5: Start daily IP Minocycline or control Day 2: 2x EdU IP Day 24: Perfusion B sham 6-OHDA + Minocycline C merge Hoechst EdU Fold increase EdU+ / total cells (sham=) 6 5 4 3 2 * sham EdU 6-OHDA + Minocycline Supplementary Figure 9

Control Mouse # Edu+ Dcx+ EdU+/Dcx+ %EdU+ %Dcx + %EdU+/Dcx+ Edu+ Dcx+ EdU+/Dcx+ % EdU+ % Dcx + %EdU+/Dcx+ 396 253 345 5823 6.8 37 5.9 9 2738 7875 264 442 23.9 68.8 22.8 77 37 57 4599 3.8 24.7 3.4 6 775 299 543 4247 8.2 5.8 2.8 7 2 555 277 5.8 26.7 4.8 8 285 867 268 359 2 63.8 9.7 4 2 727 222 6 36 4.9 6 9 3 87.7 2.9 8 3 473 66 3723 3.5 2.7.8 2 85 93 66 2476 7.5 37.6 6.7 3 39 99 93 7646 5..9 2.5 9 235 427 2 647 4.3 25.9 2.8 2 26 545 247 4387 5.9 35.2 5.6 2 394 45 379 336.9 3.6.5 22 294 76 278 2494.8 3.5. 23 42 284 28 856 6.6 33.2 5 24 95 79 72 828.7 38.8 9.4 Sum / Mean 4492 4786 47 29547 3.3 45.5.6 2367 7842 939 3472 8.6 26.9 7.4 Table S SVZ counts of EdU +, Dcx +, EdU + /Dcx + -doublepositive cells after TNF-α or control infusion TNF α Control Mouse # Edu+ Dcx+ EdU+/Dcx+ Cell % EdU+ % Dcx + %EdU+/Dcx+ Edu+ Dcx+ EdU+/Dcx+ % EdU+ % Dcx + %EdU+/Dcx+ 597 279 595 444 3.4 62.8 3.4 2 294 364 29 227 24 29.7 7.8 9 2 573 973 96 22 59.4 2.5 8 583 47 66.2 43.8 9.9 6 92 956 8 284 8.8 43.8 8.2 7 382 233 376 498 9. 48.4 9 8 233 386 76 269 8.4 3.4 3.9 4 9 34 47 25 5.6 28 2. 6 695 8 2583 3.9 26.9 3. 8 836 2322 85 3779 22. 6.4 2.3 2 552 5868 248 92 6.9 63.8 3.6 3 659 69 52 7856 8.4 2.5 6.4 9 65 334 28 788 9.2 8.7 7.2 2 245 6 24 393 6.3 25.8 5.5 2 237 87 2 362 6.5 22.3 5.8 22 29 764 8 353 6.2 2.7 5. 23 69 29 6 2 6.9 2.9 6. 24 5 352 87 735 6. 2.3 5 Sum / Mean 4989 7326 4666 34545 5. 45.5 3.2 4378 4226 3663 494 9.8 29.3 8.3 Table S2 prms counts of EdU +, Dcx +, EdU + /Dcx + -doublepositive cells after TNF-α or control infusion TNF α Sham Sham + Minocycline 6 OHDA 6 OHDA + Minocycline Mouse # EdU+ % EdU+ EdU+ % EdU+ EdU+ % EdU+ EdU+ % EdU+ 9 87 232 8.8 3 3 44 6.8 33 294 356 8.3 34 488 4449. 44 58 652 8.9 46 62 826 7.3 24 389 4838 8. 3 34 344 9. 32 246 3849 6.4 39 287 366 7.9 43 58 566.4 45 8 77.3 2 45 383.9 22 469 676 7. 29 557 5249.6 36 459 5847 7.9 4 65 323 2.8 8 68 577 3.4 2 595 683 9.6 Sum/Mean 255 2492 8.5 267 2842 8.9 9 2625 9. 927 4383 4.6 Table S3 prms counts of EdU + cells after sham or 6-OHDA injection and control or minocycline treatment, respectively

total per animal per animal 5 µm 5 5 µm >5 µm Treatment Mouse # Dcx+ Mean Dist. Median Dist. Dcx+ cells % of Dcx cells Dcx+ % of Dcx cells Dcx+ % of Dcx cells 9 255 2.3 5.3 82 94.2 73 5.82. 3 53 2.9 9. 52 99.3..65 Sham 33 6 2. 5.7 7 95.4 42 3.62.95 34 852 2.7 4.5 82 95.3 3 3.64 9.6 44 622 8.2 2.9 559 96. 56 3.45 7.43 46 3542 22.4 5. 333 93.3 2 5.93 29.82 Sum / Mean 8584 2.8 5.4 85 95.6 42 3.74 57.65 24 793 4.3.2 779 98.2.26 4.5 3 68 5.8 3. 58 99..86. Sham + Minocyclin 32 7 2.4 5.4 926 92. 78 7.75 3.3 39 298 22.3 8. 227 96.6 66 3.5 5.24 43 85 23.8 2.4 732 9.9 56 6.96 7 2. 45 838 6.5.9 795 94.9 39 4.65 4.48 Sum / Mean 679 9. 3.5 647 95.3 259 4. 33.6 2 72 7.2 4. 64 95.3 8 4.65. 22 82 22.8 8.6 2 94.8 6 5.8 2.7 6 OHDA 29 555 2. 5.3 56 97.5 26.67 3.84 36 662 24.8 2.8 58 95. 75 4.5 6.36 Sum / Mean 457 2.2 7.2 438 95.7 69 3.98 2.34 4 247 29. 6.4 2 8.4 43 7.4 3.2 6 OHDA + Minocyclin 8 56 27. 2.5 49 9.3 4 8.97.7 2 46 28.7 8.6 3 87.5 2.47 23 2. Sum / Mean 2953 28.3 8.5 263 86.4 33 2.28 37.3 Table S4 Intrastriatal immigration distances of Dcx+ cells from LV-border in Sham or 6-OHDA-lesioned and control or Minocycline treated animals 6 OHDA control 6 OHDA + NS 398 Mouse # Edu+ % EdU+ Edu+ % EdU+ 2 7 397 4.3 4 95 3936 2.4 7 72 463.7 62 53.2 3 235 4356 5.4 7 6 3654 3.2 9 24 3597 5.7 34 88 2863 3. 36 93 2982 3. 37 87 327 2.7 4 2 3847 2.9 3 2399 5.5 3 276 497 5.6 5 43 23 3.5 8 24 48 2.6 6 2946 3.9 6 323 493 7.7 2 68 3864 4.3 25 3 3772 3.4 28 69 327 5.2 29 236 4282 5.5 39 65 277 2.4 Sum / Mean 334 4589 3.2 78 38393 4.5 Table S5 prms counts of EdU + after 6-OHDA injection and control or NS-398 treatment, respectively

total per animal per animal <5µm >5µm Mouse # Dcx+ Mean Dist. Median Dist. Dcx+ cells % of Dcx cells Dcx+ cells % of Dcx cells 2 87 3.6 9 792 96.9 25 3.6 4 329 3 8.5 32 97.6 8 2.43 7 35 4.3 2.7 2 98.6 4.35 835 3.6 2 829 99.3 6.72 6 OHDA 7 3 5.9 986 98.3 7.69 9 3 24.2 3.2 6 89 24.97 34 953 2.4 9.4 925 97. 28 2.94 36 93 3.9.2 924 99.4 6.65 37 6 3.4 57 98. 3.88 4 966 4.6.3 96 97.5 5 2.54 Sum / Mean 598 4.8.9 29 97.2 38 2.82 6 OHDA + NS 398 943 23.4 2.9 829 87.9 4 2.9 3 32 7.5.2 56 93.3 76 6.7 5 268 4.9 9. 264 98.5 4.49 8 654 3.9. 646 99.5 8.48 277 8.8 5.7 258 98.5 9.49 6 2262 37.3 24. 85 79.8 457 2.2 2 22 42.5 3.2 529 69.2 68 3.8 25 257 3.4 7 24 79.4 53 2.6 28 7 4. 7.7 67 95.9 29 4.4 29 253 9.6 2.8 935 94.3 8 5.75 39 788 34.6 2.8 385 77.5 43 22.54 Sum / Mean 6858 24.3 5.9 449 88.5 2439.48 Table S6 Intrastriatal immigration distance of Dcx+ cells from LV-border in 6-OHDA-lesioned and control or NS- 398 treated animals, respectively Sham 6 OHDA + Minocycline Mouse # Edu+ % EdU+ Edu+ % EdU+ 3 2647 4.9 2 5 2782.8 3 34 2974 4.5 6 53 857 2.9 7 75 3869 4.5 8 32 5 2. 8 95 329 3. 9 68 276 2.5 2 5 3492 3. 9 5 34 5. 8 295 3.7 29 388 5.5 4 68 2763 2.5 5 263 397 6.6 7 8 362 3.5 Sum / Mean 844 252 3.3 97 9523 4.5 Table S7 Neuroinflammatory effects on Neurogenesis in aged mice. prms counts of EdU + after sham or 6-OHDA injection and control or Minocycline treatment, respectively 6 OHDA control 6 OHDA + Minocycline longterm Mouse # counts Measuretime [Min] counts per min Mouse # counts Measuretime [Min] counts per min M3 37 3.2 43.6 M 925 29.9 3. M7 252 3.7 4.7 M6 972 3.6 3.8 M8 93 3.6 39. M 988 3.3 32.6 M9 27 3.5 33.6 M2 95 29.6 32. Sum / Mean 4789 3.5 39.2 3835 3. 3.9 Table S8 Results of behavioral experiments on Rotameter after 5mg/kg injection of amphetamine in 6-OHDA lesioned mice with either longterm control or Minocycline treatment (recorded by harness measurement; 4 datapoints per turn)

6 OHDA control Dorsal Striatum Corpus Callosum Mouse # Section EdU + cells Mean EdU + cells EdU + ;GST π + cells % (EdU+;GST π+)/edu+ Mean % (EdU+;GST π+)/edu+ EdU + cells GST π + cells % GST π + cells 3 8 5 2 9.7 5 5 2 2 8 2 9 75 7 3 3.5 8 25.3 2 2 2 4 6 43 9 9 8 7 6. N/A N/A N/A 2 5 7 5 7 9 8 6 6 33 23.8 2 9 95 2 4 2 4 8 5 83 5 7 2 5.6 6 2 33 2 9 7 6 86 Sum 5 2 87 72 Mean.5.5 2 4.87 4.9 9.67 8 82 6 OHDA + Minocycline longterm Dorsal Striatum Corpus Callosum Mouse # Section EdU + cells Mean EdU + cells EdU + ;GST π + cells % (EdU+;GST π+)/edu+ Mean % (EdU+;GST π+)/edu+ EdU + cells GST π + cells % GST π + cells 5 5.5 4 27 22.7 4 4 2 6 3 9 2 83 2 6 2 3 9 26. 5 73 2 24 8 33 2 9 75 5 2 4.5 4 33 4.2 7 5 7 2 7 8 47 4 79 6 9 2 7 37 35. 4 8 57 2 2 7 33 8 7 88 N/A 4 N/A N/A 7. N/A N/A N/A 2 4 7 7 4 57 2 2 3 6 5 32. N/A N/A N/A 2 4 2 4 7 6 86 Sum 8 53 75 Mean 6.36 6.7 4.82 29.5 27.22 7.5 77 Table S9 EdU and GST- π prevalence in the striatum and corpus callosum of 6-OHDA lesioned mice with either longterm control or Minocycline treatment

Aditional Files Additional File Title: Supplementary Figures and Tables Description: this file includes supplementary figures and tables