BIOΓΡΑΦΙΚΟ ΣΗΜΕΙΩΜΑ Γεώργιος Θηραίος Ερευνητής Α

BIOΓΡΑΦΙΚΟ ΣΗΜΕΙΩΜΑ Γεώργιος Θηραίος Ερευνητής Α Ηµ/νία και τόπος γεννήσεως: 16 Νοεµβρίου 1951, Αθήνα ΕΚΠΑΙ ΕΥΣΗ - Πτυχίο Βιολογίας, Πανεπιστήµιο Αθηνών, 1974 - ιδακτορικό ίπλωµα στη Βιολογία στο Πανεπιστήµιο Harvard, Cambridge, MA, 1980 ΘΕΣΕΙΣ 1980-1982 : Μεταδιδακτορικός Ερευνητής, Πανεπιστήµιο Harvard, USA. 1982-1984 : Ερευνητής, Πανεπιστήµιο Harvard, USA. 1984-1987 : Επίκουρος Καθηγητής, Βιολογικό Τµήµα Πανεπιστηµίου Κρήτης (εκλογή - µη αποδοχή της θέσης). 1987-1992 : Ερευνητής Β βαθµίδας, Ινστιτούτο Μοριακής Βιολογίας και Βιοτεχνολογίας, Ίδρυµα Τεχνολογίας και Έρευνας, Κρήτη. 1992 - σήµερα : ιευθυντής του Ινστιτούτου Μοριακής Βιολογίας και Βιοτεχνολογίας, Ίδρυµα Τεχνολογίας και Έρευνας, Κρήτη. 1995 : Καθηγητής Μοριακής Βιολογίας, Βιολογικό Τµήµα Πανεπιστηµίου Κρήτη (εκλογή µη αποδοχή της θέσης). ΕΡΕΥΝΗΤΙΚΗ ΡΑΣΤΗΡΙΟΤΗΤΑ 1974-1980 : Μεταπτυχιακή έρευνα για ιδακτορική ιατριβή υπό την επίβλεψη του Καθηγ. Φ.Κ. Καφάτου, Πανεπιστήµιο Harvard. «Μοριακή Βιολογία της Χωριογένεσης των Εντόµων». 1980-1982 : Μεταδιδακτορική έρευνα στο εργαστήριο του Καθηγ. Φ.Κ. Καφάτου, Πανεπιστήµιο Harvard. «Μοριακή Ανάλυση της Έκφρασης των Γονιδίων του Χωρίου στη Drosophila melanogaster». 1982-1984 : Μεταδιδακτορική έρευνα υπό την επίβλεψη του Καθηγ. H. Greer, Πανεπιστήµιο Harvard. «Φυσιολογική και Μοριακή Ανάλυση του Γενικού Ελέγχου της Βιοσύνθεσης των Αµινοξέων στον Saccharomyces cerevisiae». 1984 - σήµερα : Έρευνα στο Ινστιτούτο Μοριακής Βιολογίας και Βιοτεχνολογίας του Ιδρύµατος Τεχνολογίας και Ερευνας. «Μεταγραφική Ρύθµιση της Γονιδιακής Έκφρασης στον Saccharomyces cerevisiae». ΕΠΙΣΤΗΜΟΝΙΚΗ ΠΑΡΑΓΩΓΗ 38 Πρωτότυπες ηµοσιεύσεις >2000 αναφορές Μέσο όρο impact factor: 12 (οι δηµοσιεύσεις έχουν γίνει στα περιοδικά Nature, Cell, PNAS, EMBO J, MCB, JBC και αλλού) 4 πατέντες 3 διεθνείς (µία µε την Bayer AG) και µία ελληνική ΧΡΗΜΑΤΟ ΟΤΗΣΕΙΣ >4 εκατοµµύρια Ευρώ για υποδοµές του ΙΜΒΒ >1.5 εκατοµµύρια Ευρώ για την έρευνα ΤΙΜΗΤΙΚΕΣ ΙΑΚΡΙΣΕΙΣ Εκλεγµένο µέλος της ΕΜΒΟ [1990] Τακτικό µέλος του συντακτικού συµβουλίου του EMBO Journal [1991-1994] ΙΟΙΚΗΤΙΚΕΣ ΘΕΣΕΙΣ 1988-1993 : ιευθύνων του Ινστιτούτου Μοριακής Βιολογίας και Βιοτεχνολογίας του Ιδρύµατος Τεχνολογίας και Έρευνας 1994 - σήµερα : ιευθυντής του Ινστιτούτου Μοριακής Βιολογίας και Βιοτεχνολογίας και µέλος του ιοικητικού Συµβουλίου του Ιδρύµατος Τεχνολογίας και Έρευνας 2004-2006 : Αντιπρόεδρος του Ιδρύµατος Τεχνολογίας και Έρευνας, Αναπληρωτής ιευθυντής της Κεντρικής ιοίκησης, Πρόεδρος του Τεχνικού Συµβουλίου και Προϊστάµενος της Τεχνικής Υπηρεσίας του Ιδρύµατος Τεχνολογίας και Έρευνας

ΙΠΛΩΜΑΤΑ ΕΥΡΕΣΙΤΕΧΝΙΑΣ 1. Thireos G, and Kafetzopoulos D. DNA encoding chitin deacetylase. US5525502, EP673425, CA2151257, AU5698494, AU663991, WO9413815. 11 June 1996, Licenced (Marine Biopolymers Inc, USA, KITOZYME, Belgium. 2. Thireos G. and Kafetzopoulos D. DNA encoding chitin deacetylase preparations. US6004795, (21- DEC-1999). 3. Thireos G. and Kafetzopoulos D. DNA encoding an arthropod chitin synthase. EP 97 108 240.9, US 09/230,041 JP549954/98, NO 19990244, WO9853053. 26 Nov 1998, Licensed to Bayer A.G. 4. Fotakis K, Thireos G., Zergioti I, Kafetzopoulos D. Fabrication of biopolymer patterns by means of laser transfer. GR 20010100603 (28-DEC-2001). ΕΙΣΡΟΕΣ ΑΠΟ ΕΡΕΥΝΗΤΙΚΑ ΠΡΟΓΡΑΜΜΑΤΑ Για το Ινστιτούτο: 1. Μετεγκατάσταση εργαστηριακής υποδοµής του ΙΜΒΒ [1/10/1995-31/3/1996] Προϋπολογισµός: 107.000.000 ρχ. [314.013 ] 2. Ερευνητικός Ιστός [28/11/1995-31/12/1998] Προϋπολογισµός: 300.000.000 ρχ. [880.410 ] 3. Επέκταση εξοπλισµού εργαστηρίου γονιδιωµατικών ερευνών [1/1/1999-31/12/1999] Προϋπολογισµός: 23.000.000 ρχ. [67.500 ] 4. Επέκταση υποδοµής ΙΜΒΒ-ΙΤΕ: Εξοπλισµός απαραίτητος για την εκτέλεση πειραµάτων ποσοτικής µοριακής βιολογίας [17/12/1999-17/8/2000] Προϋπολογισµός: 91.000.000 ρχ. [267.060 ] 5. Κτήριο Πειραµατόζωων [14/6/1999-31/7/2001] Προϋπολογισµός: 350.000.000 ρχ. [1.027.146 ] 6. Ολοκληρωµένη βιολογία στη µετα-γονιδιωµατική εποχή «Αριστεία» [1/1/2002-31/12/2004] Προϋπολογισµός: 500.000 7. Αριστεία 2005-2007: 946.000 Για την Ερευνητική Οµάδα: 1. ιαρθρωτικό ΓΓΕΤ - ΕΠΕΤ 20/ΚΠΣ1.4Β [1/10/1990-31/12/1993] Προϋπολογισµός: 200.000.000 ρ. [ 620.000 ] 2. Ανταγωνιστικό ΕΕ - ΒΙΟTCT90-0167 [1/1/1989-30/6/1996] Προϋπολογισµός: 75.000 3. Ανταγωνιστικό ΕΕ - CHRX-CT92-0022 [1/7/1993-30/6/1996] Προϋπολογισµός: 37.600 4. ιαρθρωτικό ΓΓΕΤ - ΕΠΕΤ 20/ΑΞΙΑ [1/1/1997-31/10/1997] Προϋπολογισµός: 15.000.000 ρχ. [44.020 ] 5. Ανταγωνιστικό ΕΕ FMRX-CT96-0064 [1/1/1996-30/9/2000] Προϋπολογισµός: 220.000 6. ιαρθρωτικό ΓΓΕΤ ΕΠΕΤ 124 [1/10/1998-31/12/2002] Προϋπολογισµός: 13.537.000 ρχ. [39.730 ] 7. ιαρθρωτικό ΓΓΕΤ ΠΕΝΕ 99Ε 456 [1/1/2000-30/6/2001] Προϋπολογισµός: 18.620.000 ρχ. [54.650 ] 8. Ανταγωνιστικό ΕΕ HPMD-CT2000-00002 [9/8/2000-8/8/2004] Προϋπολογισµός: 45.600 9. Ανταγωνιστικό ΕΕ QLK6-CT2001-51030 [3/9/2002-31/12/2003] Προϋπολογισµός: 75.200 10. ιαρθρωτικό ΓΓΕΤ ΠΕΝΕ 01Ε 509 [1/11/2002-31/10/2005] Προϋπολογισµός: 161.850 11. ιαρθρωτικό ΓΓΕΤ ΠΕΝΕ [1/2006-31/12/2008] Προϋπολογισµός: 58.796,55

ΗΜΟΣΙΕΥΣΕΙΣ Kafatos F.C., A. Efstratiadis, M.R. Goldsmith, C.W. Jones, T. Maniatis, G. Rodatkis, J.C. Regier, N. Rosenthal, G.K. Sim, G. Thireos and L. Villa-Komaroff. (1978). The developmentally regulated multigene families encoding chorion proteins in silmoths. In: Differentiation and development, Miami Winter Symposia, vol. 15, Academic Press, New York, pp. 299-315. Thireos G., R.G. Shea, and F.C. Kafatos. (1979). Identification of chorion protein precursors and the mrnas that encode them in Drosophila melanogaster. Proc. Natl. Acad. Sci. USA 74, 3851-3856. Thireos G., and F.C. Kafatos. (1980). Cell-free translation of silkmoth chorion mrnas: Identification of protein precursors, and characterization of cloned cdnas by hybrid-selected translation. Dev. Biol. 78, 36-46. Shea R.G., G. Thireos, F.C. Kafatos, W.H. Petri and L. Vill-Komaroff. (1980). Chorion cdna clones of Drosophila melanogaster and their use to study sequence homology and chromosomal location of chorion genes. Cell 19, 915-922. Nadel, M.R., G. Thireos, and F.C. Kafatos. (1980). Effect of the pleiotropic GrB mutation of Bombyx mori on chorion protein synthesis. Cell 20, 649-658. Thireos G., R.G. Shea, and F.C. Kafatos. (1980). Untranslated mrna for a chorion protein of Drosophila melanogaster accumulates transiently at the onset of specific gene amplification. Proc. Natl. Acad. Sci., USA 77, 5789-5793. Kafatos F.C., G. Thireos, C.W. Jones, S.G. Tsitilou, and K. Iatrou. (1981). Dot hybridization and hybridselected translation: Methods for determing nucleic acid concentrations and sequence homologies. In: "Gene amplification and Analysis" vol. 2, pp. 538-551. Ed. J.G. Chiizikijian and T.S. Papas. Shea R.G., G. Thireos, and F.C. Kafatos. (1982). Organization of a cluster of four chorion gene in Drosophila melanogaster and its relationship to developmental expression and amplification. Dev. Biol. 91, 325-336. Penn M.D., G. Thireos, and H. Greer. (1984). Temporal analysis of general control of amino acid biosynthesis in Saccharomyces cerevisiae: Role of postive regulatory genes in initiation and maintenance of mrna derepression. Molec. Cell. Biol. 4, 520-528. Thireos G., M.D. Penn, and H. Greer. (1984). 5' untanslated sequences are required for the translational control of a yeat regulatory gene. Proc. Natl. Acad. Sci., USA 81, 5096-5100. Tzamarias D., D. Alexandraki, and G. Thireos. (1986). Multiple cis-acting elements modulate the translational efficiency of GCN4 mrna in yeast. Proc. Natl. Acad. Sci., USA 83, 4849-4854. Kafatos F.C., C. Delidakis, W. Orr, G. Thireos, K. Komitopoulou, and Y.C. Wong. (1986). Studies on the developmentally regulated amplification and expression of Drosophila chorion genes. Molecular Developmental Biology, 85-101, Alan R. Liss, Inc. Rousou E., B. Hauge, and G. Thireos. (1988). Transcriptional-translational regulatory circuit in Saccharomyces cerevisiae, which involves the GCN2 transcriptional activator and the GCN2 protein kinase. Molec. Cell. Biol., 8, 2132-2139. Moschonas N.K., G. Thireos, and F.C. Kafatos. (1988). Evolution of Chorion structural genes and regulatory mechanisms in two wild silkmoths: A preliminary analysis, J. Mol. Evol. 27, 187-193. Tzamarias D., and G. Thireos. (1988). Evidence that the GCN2 protein kinase regulates reinitiation by yeast ribosomes. EMBO J. 7, 3547-3551. Tzamarias D., Rousou E., and G. Thireos. (1989). Coupling of GCN4 mrna translational activation with decreased rates of polypeptide chain initiation in yeast cells. Cell 57, 945-754. Krupitza G., and G. Thireos. (1990). Translational activation of GCN4 mrna in a cell free system is triggered by uncharged trnas. Molec. Cell. Biol. 10, 4375-4378. Georgatsou E., T. Georgakopoulos and G. Thireos (1992). Cloning of an essential gene encoding a proteasomal subunit in yeast. FEBS Letters 299: 39-43. S.G. Oliver,... (6) D. Alexandraki,... (119) G. Thireos, M. Tzermia,... (137) J.G. Sgouros (1992). The complete DNA sequence of yeast chromosome III. Nature 7: 38-46 Georgakopoulos T. and G. Thireos (1992). Two distinct yeast transcriptional activators require the function of the GCN5 protein to promote normal levels of transcription. EMBO J. 11, 4146-4152. Kafetzopoulos, D., G. Thireos, J.N. Vournakis and V. Bouriotis. (1993). The primary structure of a fungal chitin deacetylase reveals the function for two bacterial gene products. Proc. Natl. Acad. Sci. USA 90: 8005-8008. Diallinas, G. and G. Thireos (1994). Genetic and biochemical evidence for yeast GCN2 protein kinase polymerization. Gene 143: 21-27. Gounalaki, N. and G. Thireos (1994). Yap1p, a yeast transcriptional activator that mediates multidrug resistance, regulates the metabolic stress response. EMBO J. 13 4036-4041.

Georgakopoulos, T., Gounalaki, N. and G. Thireos (1995). Genetic evidence for the interaction of the yeast GCN5 and ADA2 transcriptional co-activator proteins. Mol. Gen. Genet. 246, 723-728 Tavernarakis N. and Thireos G. (1995). A recombinatorial method useful for cloning dominant alleles in Saccharomyces cerevisiae. Nucl. Acids Res. 23, 537-538 Tavernarakis N. and Thireos, G. (1995) Transcriptional interference by GCN4 overexpression reveals multiple interactions mediationg transcriptional activation. Molec. Gen. Genet. 247, 571-578. Kyrpides N., Papamatheakis S., Tavernarakis N. and G. Thireos (1995) A transient GCN4 mrna destabilization follows GCN4 translational derepression. J. Biol. Chem. 270, 17317-17320. Christodoulidou A., Bouriotis V. and G. Thireos (1996) Two sporulation-specific chitin deacetylaseencoding genes are required for the ascospore wall rigidity of Saccharomyces cerevisiae. J. Biol. Chem. 271, 31420-31245. Tavernarakis, N. and G. Thireos (1996) Genetic evidence for functional specificity of the yeast GCN2 kinase. Mol. Gen. Genet. 251, 613-618. Tavernarakis, N., Alexandraki, D., Liodis, P., Tzamarias, D. and G. Thireos (1996) Gene overexpression reveals alternative mechanisms tha induce GCN4 mrna translation. Gene 179, 271-277. Tavernarakis N. and G. Thireos (1997) The DNA target sequence influences the dependence of the yeast transcriptional activator GCN4 and co-factors. Mol. Gen. Genet. 253, 766-769. Goffeau, A., Alexandraki, D., Horaitis, O., G. Thireos, Tzermia, M. (1997) The yeast genome directory. Nature 387 (suppl.). Syntichaki, P. and Thireos, G. (1998). The Gcn5/Ada complex potentiates the histone actyltransferase activity of Gcn5. J. Biol. Chem. 273, 24414-24419. Benos, P., Tavernarakis, N., Brognia, S., Thireos, G., Savakis, C. (2000). Acquisition of a potential marker for insect transformation: isolation of novel alcohol dehydrogenase gene from Bacterocera oleae by functional complementation in yeast. Mol. Gen. Genet. 263, 90-95. Syntichaki, P., Topalidou, I. and Thireos, G. (2000). The Gcn5 bromodomain co-ordinates nucleosome remodelling. Nature 404, 414-417. Topalidou, I. and Thireos, G. (2003). GCN4 occupancy of open reading frame regions results in the recruitment of chromatin-modifying complexes but not mediator complex. EMBO Rep. 4, 872-876. Topalidou, I., Papamichos-Chronakis, M. and Thireos, G. (2003). Post-TATA binding protein recruitment clearance of Gcn5-dependent histone acetylation within promoter nucleosomes. Mol. Cell. Biol. 23, 7809-7817. Topalidou, I., Papamichos-Chronakis, M., Thireos, G., Tzamarias, D. (2004). Spt3 and Mot1 co-operate in nucleosome remodelling independently of TBP recruitment. EMBO J. 23, 1943-1948 Martin-Sanchez F, Iakovidis I, Norager S, Maojo V, de Groen P, Van der Lei J, Jones T, Abraham-Fuchs K, Apweiler R, Babic A, Baud R, Breton V, Cinquin P, Doupi P, Dugas M, Eils R, Engelbrecht R, Ghazal P, Jehenson P, Kulikowski C, Lampe K, De Moor G, Orphanoudakis S, Rossing N, Sarachan B, Sousa A, Spekowius G, Thireos G, Zahlmann G, Zvarova J, Hermosilla I, Vicente FJ. (2004) Synergy between medical informatics and bioinformatics: facilitating genomic medicine for future health care. J Biomed Inform. 2004 Feb;37(1):30-42. Gligoris T, Thireos G, Tzamarias D. (2007)The Tup1 corepressor directs Htz1 deposition at a specific promoter nucleosome marking the GAL1 gene for rapid activation. Mol Cell Biol. 27(11):4198-205.

Presented Papers: Thireos G. (1978). Identification of chorion genes by hybrid-selected translation. International Conference on Molecular and Developmental Biology of Insects. Crete, Greece. Thireos G. (1980). Transient transcription of a Drosophila chorion gene during gene amplification. (a) EMBO Workshop on Molecular Biology and Genetics of Drosophila melanogaster. Crete, Greece. (b) INSERM Conference on Structure and Expression of Eucaryotic Genomes. Paris, France. Thireos G. (1981). Chorion gene amplification and transcription. Cold Spring Harbor Conference on Gene Amplification and Aberrant Chromosome Structure, October1981. Thireos G. (1982). Chorion gene structure and expression. EMBO Workshop on Molecular Biology and Genetics of Drosophila melanogaster, Crete. Thireos G., M.D. Penn and H. Greer. (1983). General control of amino acid biosynthesis involves the coordinate action of initiation and maintenence genes. Cold Spring Harbor Conference on Molecular Biology of Yeast, August 1983. Penn M.D., G. Thireos, and H. Greer. (1983). Cloning and molecular analysis of postive regulatory genes involved in the general control of amino acid biosynthesis. Cold Spring Harbor Conference on Molecular Biology of Yeast, August 1983. Penn M.D., G. Thireos, and H. Greer. (1984). Translational control of GCN4 mrna in Yeast. Internationl Conference on Yeast Molecular Biology and Genetics. Edinburgh, August 1984. Thireos G., D. Alexandraki and D. Tzamarias. (1986). Involement of cis and trans acting elements in translational regulation. UCLA Symposia on Molecular Mechanisms in Development. Keystone Colorado, April 1986. Thireos G. (1986). Specific mrna binding proteins and translational regulation in yeast. EMBO workshop on Regulation of Protein Synthesis in Eucaryotes. Patra, Greece, September 1986. Rousou E., Tzamarias D., Hauge, B., and Thireos G. (1988). Translational derrepresion of the GCN4 mrna is the result of a general block in translational initiation. Fourteenth International Conference on Yeast Genetics and Molecular Biology, Helsinki Finland. Alexandraki D., Galant P., and Thireos G. Sequencing part of chromosome 3 from S. cerevisiae. Meeting on Genome Mapping and Sequencing. Cold Spring Harbor Laboratory, CSH, New York, April 1989. Thireos G., D. Tzamarias, E. Rousou and G. Krupitza. (1989). Translational regulation of GCN4 mrna. Cold Spring Harbor Laboratory Meeting on Translational Control, September 1989. Alexandraki D., and Thireos G. Sequencing part of chromosome II from S. ceravisiae. BAP meeting on the Sequencing of the Yeast Chromosome III. Tutzing Obb., FRG, November 1989. Thireos G., Alexandraki D., Dialynas G., Krupitza, G., Maniataki E., and Tzamarias D. Translational regulation of GCN4 mrna: in vivo and in vitro approaches. NATO/EEC Workshop on Posttranscriptional Control of Gene Expression, Goslar, West Germany, April 1990. Thireos G., D. Alexandraki, T. Georgakopoulos, G. Dialinas, G. Krupitza, E. Maniataki, I. Rousou, D. Tzamarias and N. Tzortzakis. Translational regulation of GCN4 mrna and the control of protein synthetic rates in Yeast. ICGEB Colloquium on Eucaryotic Gene Regulation and Expression, Crete, May 1990. Alexandraki D., Tzamarias D., and G. Thireos. (1990). High levels of undarged trna overside the known signaling pathwons for translational activation of the GCN4 mrna. 15th International Conference on teast Genetics and Molecular Biology, The Hague, July 1990.Thireos, G., T. Georgakopoulos, D. Alexandraki, E. Maniataki and N. Tavernarakis. (1992). Translational and Transcriptional regulation in Yeast: 16th International Conference on Yeast Genetics and Molecular Biology, Vienna, August 1992. Thireos, G., T. Georgakopoulos, D. Alexandraki, E. Maniataki and N. Tavernarakis. (1992). Translational and Transcriptional regulation in Yeast: The GCN4 model. Control of Gene Expression in Yeast, Instituto Juan March, Madrid, December 1992. Georgakopoulos, T., Gounalaki, N. and Thireos, G. (1993). Transcriptional coactivators - Function of GCN5 and ADA2 proteins in the GCN4-mediated transcriptional activation in yeast. Cold Spring Harbor Meeting on Mechanisms of Eukaryotic Transcription, September 1993.