Kiyofumi OHKUSU 45 35 10 microbe 1879 19 20 1876 1880 1882 1883 1884 1894 1905 2002 MALDI-TOF MS 500 2011 taxonomy taxis = arrangement nimina = distribution Cowan 3 classification nomenclature identification 0501-1194 1-1 Department of Microbiology, Gifu University Graduate School of Medicine (1-1 Yanagido, Gifu)
8,000 100 International Code of Nomenclature of Bacteria ; ICNB strain species subspecies serovar phagovar pathovar biovar binomial nomenclature genus 2 specific epithet 1 DNA DNA DNA 70 5 1 DNADNA 20 1970 DNA GC 1980 16S rdna 16S rdna 1,500 bp 98.7 2 16S rrna 5
Mass Spectrometry Mass Spectrometry MS 3 MALDI-TOF MSMatrix Assisted Laser DesorptionIonization -Time of Flight Mass Spectrometer MALDI-TOF MS MALDI-TOF MS Matrix Assisted Laser DesorptionIonization Time of Flight Mass Spectrometer ; TOF MS 3 MALDI 337nm α-cyano-4- hydroxy cinnamic acidchca sinapinic acid - COOH H MALDI EI CI FAB ESI MALDI TOF
MALDI Laser DesorptionIonization 1985 3 2002 1 1 K= mv 2 2 mv MALDI-TOF MS MS 1 2 1 MALDI Biotyper mz
MALDI-TOF MS BD 3 1 AXIMA VITEK MS MALDI BiotyperVITEK MS MALDI-TOF MS MALDI-TOF MS 3 10 5 1 MALDI-TOF MS 2,000 20,000 Da 50 70 6 2 5 MALDI-TOF MS
MALDI BiotyperVITEK MS 1, 2 MALDI Biotyper Main SpectraMSP 2.0 1.7 2.0 VITEK MS SuperSpectra TM
MALDI Biotyper 15 4 3 MALDI-TOF MS 10 MALDI-TOF MS 2 10 1 20 50
MALDI Biotyper MALDI Biotyper 2,800 VITEK MS 2,980 MALDI-TOF MS Escherichia coli Shigella flexnerimycobacterium tuberculosis M. bovis Bacillus cereus B. anthracis Yersinia pestis Y. pseudotuberculosis DNADNA 70 5 7 70 95 80 98 Staphylococcus 90 Streptococcuss S. pneumoniae S. mitis grroup MALDI-TOF MS 16S rdna MALDI-TOF MS MALDI-TOF MS 1 2012 1 MALDI Biotyper 2011 100 Mycobacterium
Candida Cryptococcus 8, 9 MALDI-TOF MS 10 12 70 80 Bruker MALDI Sepsityper MALDI-TOF MS MALDI Sepsityper TM 5 1 800 10 5 13, 14 MALDI-TOF MS 15 2 51 16, 17-18, 19 MALDI-TOF MS 20 Family Genes Species Strain DNA-DNA 16S rrna GC mol PFGE Multilocus sequence typingmlst MALDI-TOF MS
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