(// // ).. LMX MPN/100ml MTF LMX. MTF. MPN/100ml LMX... LMX : Comparison of Enzymatic Assay and Multiple Tube Fermentation Technique in the Assessment of Microbial Quality of the Karoon River Mahnaz Nikaeen 1 Afsaneh Pejhan 2 Mohammad Jalali 3 Akbar Hassan Zadeh 4 (Received July 8, 2008 Accepted May 1, 2010) Abstract Microbiological monitoring of surface waters designated for use as drinking water is essential by water utilities for the design and operation of drinking water treatment plants. Enzymatic assays have been applied as a rapid alternative approach to assess the microbiological quality of freshwater. In this study, the LMX broth (LMX) as an enzymatic assay was compared with the standard method of multiple tube fermentation technique (MTF) for the microbial monitoring of the Karoon River. Enumeration of total coliforms and E. coli averaged 9928 and 6684 MPN/ 100 ml by the LMX and 7564 and 6546 MPN/ 100 ml for the MTF, respectively. This difference was statistically significant for TC but the overall analysis revealed no difference between E. coli recoveries on LMX and MTF. In conclusion, LMX can be used for the enumeration of coliforms and E. coli in surface waters as it is less lobar-intensive, yields faster result, and simultaneously detects both total coliforms and E. coli. Keywords: Microbiological Quality, Surface Water, Karoon, Multiple Tube Fermentation, Enzymatic Assay, LMX. 1. Assist. Prof. of Environmental Health, Faculty of Public Health, Isfahan University of Medical Sciences, Isfahan (Corresponding Author) (+98 311) 7922660 nikaeen@hlth.mui.ac.ir 2. M. Sc. of Environmental Health, Public Health Center, Yasooj 3. Assoc. Prof., Dept. of Nutrition Isfahan Uvinversity of Medical Sciences, Isfahan 4. Instructor of Statistics, Academic Member of Public Health, Isfahan University of Medical Sciences, Isfahan - nikaeen@hlth.mui.ac.ir () ( ) - - - 58
... - - ].[....[ ] LMX. -. () (MTF) -- 7 Flourocult LMX 8 Merck -.. (FC) (TC).[ ].[- ] (MTF). MTF (MF).[ ].[ ].. (VNCB).[ ].[ ] 1 Total Coliforms (TC) 2 Fecal Coliforms (FC) 3 Escherichia Coli (E.Coli) 4 Multiple Tube Fermentation (MTF) 5 Membrane Filter (MF) 6 Viable But Nonculturable Bacteria (VNCB) 59
.[]. MPN/100ml.[] ph. -. MTF LMX MPN/100ml. MPN/100ml.. EC (BGLB).[] LMX / -- LMX.. UV. 1 Lactose Broth 2 Brilliant Green Lactose Bile Broth (BGLB) (MTF) (LMX) - (MPN/100ml) LMX (MPN/100ml) MTF (MTF) (LMX) - (MPN/100ml) LMX (MPN/100ml) MTF 60
LMX. MTF LMX / MTF LMX.(P<0.05) MTF /. MTF. LMX. ( ). ( )... / /.(P<0.05).. LMX (MPN/100 m l) LMX (MPN/100 ml) 16000 14000 12000 10000 8000 6000 4000 2000 0 16000 14000 12000 10000 8000 6000 4000 2000 0 R2=0.8 0 2000 4000 6000 8000 10000 12000 14000 16000 MTF (MPN/100 ml) (MTF) - (LMX) R2=0.807 0 2000 4000 6000 8000 10000 12000 14000 16000 MTF (MPN/100 ml) (MTF) - (LMX)..( ) (mg/l) 2 ± - ( o C) (µmhos/cm) ± (NTU) ± ph 1 /-/ / ±/ ± ± ± -/ / ±/ ± ± ± -/ / ±/ ± ± ± -/ / ± / ± ± ± /- / ± / ± ± ± /- / ± / ph - -. 61
.[ ] (ABNC).[ ] ABNC.[ ].[]. MTF LMX ( ) / MTF MTF.[ ] LMX. - LMX. MTF. 4 Active But Nonculturable Bacteria (ABNC) 5 George et al. 6 Seine 7 Kellebsilla LMX.. MTF. LMX MTF.[].. MF MTF.[] MTF LMX. /.[].[ ] MTF ( ) LMX MTF 1 Geissler et al. 2 Microalage 3 Aeromonas - 1- Clark, D.L., Milner, B.B., Stewart, M.H., Wolfe, R.L., and Olson, B.H. (1991). Comparative study of commercial 4-methylumbelliferylb-D-glucuronide preparations with the standard methods membrane filtration fecal coliforms test for the detection of Escherichia coli in water samples. Appl. Environ. Microbiol, 57 (5), 1528-1534. 62
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