Regulation of Primary Metabolic Pathways in Oyster Mushroom Mycelia Induced by Blue Light Stimulation: Accumulation of Shikimic Acid Masanobu Kojima*, Ninako Kimura & Ryuhei Miura Department of Bioscience and Biotechnology, Shinshu University, Kamiina, Nagano 99-598, Japan. *Correspondence and requests for materials should be addressed to M.K. (mkojima@shinshu-u.ac.jp).
Legends: Supplementary Fig. S1. PLC analysis of shikimic acid in oyster mushroom mycelia cultivated in the dark (before irradiation) and under blue light stimulation for h. Supplementary Fig. S. Comprehensive analysis of gene expression in oyster mushroom mycelia after blue light stimulation using a custom microarray: PFD, 150 µmol m - s -1 ; stimulation time, 0, 0.5, 1,,, 1, and h. a h. Altered transcripts were quantified using the comparative method. Genes with greater than or equal to -fold changes in signal intensity were used to identify significant differences in gene expression. Differentially expressed genes were also subjected to K-means analysis and were classified into eight clusters (I VIII). Total number of genes (TNG) categorized in each cluster is shown in a h. Supplementary Fig. S. CA of the time course of gene expression related to aromatic amino acid biosynthesis, glycolysis/gluconeogenesis and the pentose phosphate pathway. Supplementary Fig. S. Glycolysis pathway. EC.7.1.11 (PFK) is a rate-determining enzyme.
Supplementary Fig. S5. Pentose phosphate pathway. EC1.1.1.9 (GPD) is a rate-determining enzyme. Supplementary Fig. S. Time course of the expression levels of the rate-determining enzymes as detected by antibody response. The expression levels of the enzymes were analyzed using ImageJ software; n = ; error bar = SD. Supplementary Table S1. CA of the time course induction of 1 primary metabolites by blue light stimulation Supplementary Table S. Probe names of genes represented in the custom microarray, EC number and the KEGG Orthology (KO) ID of the corresponding protein Supplementary Table S. Forward and reverse primers synthesized for quantitative PCR analysis Supplementary Table S. Peptides synthesized for preparation of polyclonal primary antibodies against the target proteins
Before irradiation Analysis Conditions: Column: Inertsil ILIC (GL Sciences Ltd.) Particle Size: 5 μm Length: Φ. x 50 mm Detection UV Wavelength: 10 nm Mobile Phase: 1% PO /MeCN = 10/90 Flow Rate: 0. ml/min Oven Temp.: 0 o C Injection Amount: 10 μl Retention Time of Shikimic Acid: 11.5 min After blue light stimulation for h Supplementary Fig. S1
a Expression level 7 5-1 01 - - - -5 - Cluster I (TNG: 110) b Cluster II (TNG: 75) 0 0.5 1 1 Stimulation time (h) Expression level 7 5-1 01 - - - -5-0 0.5 1 1 Stimulation time (h) c Expression level 7 5-1 01 - - - -5 - Cluster III (TNG: 7) 0 0.5 1 1 Stimulation time (h) Supplementary Fig. Sa-d d Expression level 7 5-1 01 - - - -5 - Cluster IV (TNG: 189) 0 0.5 1 1 Stimulation time (h)
e Expression level 7 5-1 01 - - - -5 - Cluster V (TNG: 119) 0 0.5 1 1 Stimulation time (h) f Expression level 7 5-1 01 - - - -5 - Cluster VI (TNG: 55) 0 0.5 1 1 Stimulation time (h) g Expression level 7 5-1 01 - - - -5 - Cluster VII (TNG: 5) 0 0.5 1 1 Stimulation time (h) Supplementary Fig. Se-h h Expression level 7 5-1 01 - - - -5 - Cluster VIII (TNG: 115) 0 0.5 1 1 Stimulation time (h)
CA of the Time Course of Gene Expression Related to Phenylalanine, Tyrosine, Tryptophan Biosynthesis (Cf. KEGG map0000) Color range of expression level a ierarchical clustering on genes associated with the enzymes ierarchical clustering on the (time-series) samples EC number a Red and green cells indicate the ratio of each expression level above and below the median, respectively. Supplementary Fig. Sa
CA of the Time Course of Gene Expression Related to Glycolysis/Gluconeogenesis (Cf. KEGG map00010) Color range of expression level a ierarchical clustering on genes associated with the enzymes ierarchical clustering on the (time-series) samples a Red and green cells indicate the ratio of each expression level above and below the median, respectively. Supplementary Fig. Sb EC number
CA of the Time Course of Gene Expression Related to the Pentose Phosphate Pathway (Cf. KEGG map0000) Color range of expression level a ierarchical clustering on genes associated with the enzymes ierarchical clustering on the (time-series) samples a Red and green cells indicate the ratio of each expression level above and below the median, respectively. EC number Supplementary Fig. Sc
C O O O O O O Glucose (Glc) O O C OPO O O O Glucose -phosphate (GP) O C O O O O C OPO Fructose -phosphate (FP).7.1.11 O C OPO O O O C OPO Fructose 1,-bisphosphate (F1,BP) C OPO O C O Dihydroxyacetone phosphate (DAP) O PO COO - Phosphoenolpyruvic acid (PEP) O O - OPO C O -Phosphoglyceric acid (PG) O O - O C OPO -Phosphoglyceric acid (PG) O OPO O C OPO 1,-Bisphosphoglyceric acid (1,BPG) O O C OPO Glyceraldehyde -phosphate (GAP) O CO - Pyruvic acid (Pyr) Supplementary Fig. S
C O O O O O O Glucose (Glu) O O C OPO O O O Glucose -phosphate (GP) 1.1.1.9 O O C OPO O O O -Phosphogluconolactone O CO - O O O C OPO - -phosphogluconic acid O CO O O C OPO Erythrose -phosphate (EP) + C O O O O C OPO - Fructose -phosphate (FP) O C O O O O O C OPO - Sedoheptulose 7-phosphate (S7P) + CO O C OPO Glyceraldehyde -phosphate (GAP) O CO O O O C OPO Ribose 5-phosphate (R5P) + C O O O C OPO Xylulose 5-phosphate (Xu5P) C O O O O C OPO Ribulose 5-phosphate (Ru5P) Supplementary Fig. S5
a EC.7.1.11: PFK b EC1.1.1.9: GPD PFK expression 1 1 10 8 0 0 1 Light stimulation (h) GPD expression 1 10 8 0 0 1 Light stimulation (h) EC.5.1.5: DAPS c d e 10881 expression 1 10 8 0 0 1 Light stimulation (h) 108 expression 50 0 0 0 10 0 0 1 Light stimulation (h) 10910 expression 10 10 100 80 0 0 0 0 0 1 Light stimulation (h) Supplementary Fig. S
Supplementary Table S1. CA of the Time Course Induction of 1 Primary Metabolites Induced by Blue Light Stimulation NO. Compound name Blue-Light Stimulation Time vs Standardized Score Dark 0 h h 1 h h h h 1 γ-glu--aminobutyric acid.0-0.9-0. -0.50-0.7-0. Phosphorylcholine.0-0. -0.0.57-0. -0. XC001 (Unknown).0-0. -0.58-0.7-0. -0.8 NADP_divalent.00. -0.9-0.5-0.5-0.18 5 N-Acetylglutamic acid.00.17-0.50.58-0. -0.9 Citrulline.01-0.15-0.7-0.58-0.50-0.51 7 -Isopropylmalic acid.01-0.9-0.9-0.55-0.50.0 8 -Aminobutyric acid 1.99-0.18-0.0-0.80. -0.5 9 XA00 (Unknown) 1.98-0.9-0.1-0.70.5-0.0 10 -ydroxybutyric acid 1.97 0.00-0.9-0.0-0. -0. 11 N-Acetyllysine 1.97-0. -0.11-0. -0.1-0.75 1 S-Adenosylmethionine 1.97-0.9-0. -0.78-0.9 0.0 1 N-Acetylornithine 1.97-0.09-0. -0.9-0.7-0. 1 Fructose 1,-diphosphate 1.9 0.01-0.8-0.71-0.8-0.1 15 Cystathionine 1.9-0.0-0.07-0.89-0.5-0. 1 Adenylosuccinic acid 1.9-0. -0.5-0.59-0.0 0. 17 Lys 1.9-0.5 0.10. -0.70.7 18 myo-inositol -phosphate 1.91-0.98-0.1-0.07-0.01-0.5 19 Saccharopine 1.91 0.5-0.9-0.9-0.75-0. 0 ypotaurine 1.89-0.17-0.5-1.07 0.05-0.5 1 exanoic acid 1.89-0. -0. -0.58-0.78 0. N-Acetylglucosamine -phosphate 1.89-0.19-0.5-0.8-0.7 0.5 Argininosuccinic acid 1.89-0.9-0.58-0.5-0.70 0. Uric acid 1.8-0.7-0. -0.7-0.0 0.8 5 Ornithine 1.8-0.55-0. 0.1-1.05-0.09 5-Aminovaleric acid 1.80.0 0.5-0.1-0.5-1.01 7 Betaine 1.81-0.1-0.78-0.51-0. 0.5 8 β-ala 1.78-0.0-0.70-0.80-0. 0.58 9 Ophthalmic acid 1.77-0.05-0.00.7 0.1-1.17 0 Choline 1.75-0.0.1-1.7 0.1-0. 1 Glucose 1-phosphate 1.75-0.1-0.50.7-0.87 0.58 is 1.75-0.10 0.5-0.70.50.9 N-Acetylserine 1.71 0.0.55-0.8-1.07 0.7 UMP 1.9 0.5-0.9-0.81-1.1 0.9 5 Betaine aldehyde_+o 1.8 0.0 0.05-0.90 0. -1.1 Glucose -phosphate 1.8 0.1-0.8-0.9-1.0 0.1 7 m-ydroxybenzoic acid 1.8-0.7-0.7-0.80-0.19 0.7 8 Butyrylcarnitine 1.7 0.5-0.8-1. -0.7 0.18 9 Arg 1.7 0.11-0.7 0.09-1.5-0.1 0 ADP-glucoseGDP-fucose 1.5-0.07-0. -0.98-0.9 0.59 1 -Aminobutyric acid 1.5 0.8 0.8-0.70-0.79-0.9 O-Phosphoserine 1. -0. -0.0.9-0.88 0.80 S-Adenosylhomocysteine 1.1-0.90-0.1-0.07 0.70.95 Threonic acid 1.0-0.17-0.78-0.89-0.58 0.8 5 Fructose -phosphate 1.0 0.17-0.0.99-1.0 0.50 Sucrose '-phosphate 1.59-0.09-0.5-0.81-0.95 0.79 7 Uracil 1.58-1.0-1.1 0.0 0.19 0.5 8 -Phosphoglyceric acid 1.58 0.9-0.5-1.00-0.95 0.0 9 N,N-Dimethylhistidine 1.57 0.1 0.5-0.8-0.8-1.11 50 Carboxymethyllysine 1.5 0.08 0.9-0.0-0.11.9 51 -Aminoisobutyric acid 1.5 0.1-0.8-1.09-0.5 0.70 5 omocitrulline 1.50 0.8 0.55-0.19-1.11-1.0 5 XC0089 (Unknown) 1.9-0.9-0.1-0.0-0. 1.07 5 Glycerol -phosphate 1. 0.11-0.85-0.1.1 0.8 55 CDP-choline 1.1-0. -0.5-0.71.0 1.05 5 Pro 1.8-0.09 0. -0.0 0.0-1.5 57 Dihydroxyacetone phosphate 1. 0.95-0.11-1.18-0.95-0.05 58 UDP-glucoseUDP-galactose 1. 0.01-0. -0.97-0.99 1.0 59 Ergothioneine 1.1-0.1-0.5-1.0-0.77 1.1 0 myo-inositol 1-phosphate myo-inositol -phosphate 1.9 0.18-0.90-0.0-1. 0.95 1 UDP-glucuronic acid 1.9 0.19-0. -1.11.0 0.9 Sedoheptulose 7-phosphate 1. 0.10.0-0.87-1.17 1.0 Uridine 1. -1.0-1.17 0.71 0. 0.15 N-Acetylneuraminic acid 1. -1.1-0.8 0.78-0. 0. 5 -Phosphoglyceric acid 1. 1.07-0.09-1. -0.90-0.08 omoserine 1.0.90.1-0.07 1.10-1.0 7 datp 1.10. -0. -0. -0. 1.5 8 dtdp 1.10.88 0.0.88-0.88 1.11 9 istidinol 1.0-0.8-0.78-0.71-0.57 1.8 70 Erythrose -phosphate 1.05 0.09-0.0 0.05-1.75 0.8 71 Sebacic acid 1.0-0.0.79-0.55-0.58 1.51
7 GDP 1.0 0. 0.5-0.98-1.8 0.7 7 UDP 0.99 0.81-0.01-1.1.1 0.5 7 dttp 0.98-0. -0. -0. -0. 1.5 75 ydroxyproline 0.98 0.7 0. 0.9-0.0-1.9 7 Cys 0.9-0.0 1. -1.1-0. -0.71 77 Adenine 0.9 0.0-1.11.1 1.5-0.15 78 GMP 0.9-1.1-0.8 1.18 0.7-0.9 79 10-ydroxydecanoic acid 0.89-0. -0.77-0.57-0.80 1.59 80 CoA_divalent 0.8-0.8 1.5-1.10-0.7-0. 81 UTP 0.8 0.5-0.8-1.15-1.1 1.1 8 Citric acid 0.85 0.05-0.0-1.11-0.85 1.7 8 Ribulose 5-phosphate 0.78 0.88-0.70.9-1.0 1.05 8 Glutathione (GS) 0.75-0.5-0.50.9-0.8 1.9 85 GABA 0.7-0.71-1.5-0.8 1.0 0.70 8 -Aminoadipic acid 0.71-0. 1.0.17-1.0-0.81 87 -Phosphogluconic acid 0.71 0.07 0.08-0.81-1.1 1. 88 N-Formylaspartic acid 0.71-0. 0.57 0.8-1.88 0.7 89 Pantothenic acid 0. -0.1-0.80-0.9-0.0 1.75 90 O-Succinylhomoserine 0.58 0.79 0.89 0.0-0.95-1.5 91 Val 0.5-0.10 0.0-0.5 1.1.5 9 GTP 0.5 0.9 0.05-1.07-1. 1.1 9 Creatine 0. 0.1-0.7-0.91-0. 1.7 9 Ethanolamine 0.8-0.9-1.1 0.1 0. 1. 95 ATP 0. 0. 0.17-1.0-1. 1. 9 Malic acid 0. 0.00.9-0.5-1.1 1.80 97 N-Acetylaspartic acid 0.1-0.50-0.8-0.58 1.95-0.59 98 Cadaverine 0.19 0.89 1.01-0.95 0. -1.7 99 FAD_divalent 0.18-0. -0.7-0. -0.1 1.9 100 Trp 0.18-0.7 0.00.0-0.7 1.88 101 -Methylserine 0.1-0.9-0.80-0.71-0.15 1.9 10 ADP 0.10 0. 0.77-0.1-1.7 0.79 10 Glucuronic acid 0.07-0.5-0.07-0.0-1.10 1.8 10 N-Acetylasparagine 0.0 0.80 0.51-0.81.55 1.01 105 Phe 0.0 0. 0.1 0.1.9 1.01 10 Leu 0.0-0.1 0. -0.10 1.5-1.0 107 Cytidine -0.0 0. 0.7 1. -1. -1.10 108 Carnitine -0.0-0.0.7-0.7-0.1 1.95 109 Asp -0.0 0. 0.01.7 1.7-0.01 110 N-Acetylglycine -0.0-0.8-0.9-0..01-0.55 111 cgmp -0.09-0.0-0.9 0.51.07 1.7 11 dtdp-glucose -0.10 0.59 0. -1.10-1. 1. 11 Fumaric acid -0.11 0.7-0.8-0.57-1.1 1.80 11 -Methyl--oxovaleric acid -Methyl--oxovaleric acid -0.1 0.8-0.5 0. 1.9-1.9 115 CTP -0.1 1.7 0. -1.10-1.1 0.7 11 UDP-N-acetylglucosamine -0.1 0.79 0.8-1.05-1. 1.1 117 XA000 (Unknown) -0.1-1.9 0.9 0.7 0.5 0.7 118 CDP -0. 1.5 0.80.9-1.1 0.0 119 Trigonelline -0. -0.51.17-0.8 0. 1. 10 Ribose 5-phosphate -0. 1.89-0.0-0.70-0.95 0.0 11 N-Acetylmethionine -0. 0.0-0.5 1.7-1. 0.8 1 Terephthalic acid -0.5-0.55 1.95-0.01-0.87-0.7 1 NMN -0.7-0.50.8 0.1-0. 1.9 1 Gln -0.9-0.51-0. 0.15-0.91 1.9 15 Met -0.0.50-0.0..01-0.7 1 Spermidine -0.1 0.1-0.17-0.7-0.8 1.91 17 camp -0.0.71-0.71 1.0-0.71 1.8 18 -ydroxy--methylglutaric acid -0. -0.55-0. 0.00 1.97-0.77 19 Tryptamine -0.5-0.0. -0.08-0.17 1.98 10 Oxypurinol -0.5-0.5-0.5-0.15.00. 11 5-Oxoproline -0.9-0.9-1.5 1.7 0.95-0.09 1 N-Acetylleucine -0.50-0.79-0.8 0.87 1.0-0.80 1 Pipecolic acid -0.51 0.1 1.8 0.97-0.5-1.0 1 Trimethylamine -0.50. -0.70 0.7 1.70.58 15 Trehalose -phosphate -0.5 1.00 0.9-1. 1.15-0.5 1 -Guanidinobutyric acid -0.5-0.7-0.71 0.8-0. 1.87 17 XC000 (Unknown) -0.5-0.01-0.00.08-1.1 1.8 18 -Methylhistidine -0.59-0.07 0.7 0.5-1.5 1.9 19 Isocitric acid -0.0-0.50.08-0.7.01-0. 10 CMP-N-acetylneuraminate -0.0-1.18-0.0 0.9 1.1-0.11 11 β-ala-lys -0.1-0.7-0.77 1. -0.5 1. 1 Phenylpyruvic acid -0.0.58-0.0-0.1.00-0.10 1 ccmp','-ccmp -0.0.0. 1. -0. 0.8 1 XA000 (Unknown) -0. 0.0-0.5 0.99-1.1 1.7 15 Glucosaminic acid -0.5-0.8-0.80 0.0 1.7 0.1 1 Galacturonic acid -0.7-0.75 0.0 0.7 1. -0.9 17 -ydroxypropionic acid -0.8-0.78 0. 1.8-0. -0.58 18,-Diaminobutyric acid -0.8 0.9-0.57 1.1-1.1 0.0
19 -Phenylethylamine -0.9-0.7-0.5 1.99-0.01-0.7 150 Lactic acid -0.71-0. -0.7-0. -0..01 151 SDMA -0.71.05 0.5 1.0 1.7-0.78 15 Inosine -0.75-0. 0.05 0. 1.1.09 15 AMP -0.78-0. 0.5 1.81-0.0-0.50 15 Putrescine -0.78 1.08 0.50 1.08-0.85-1.0 155 Ile -0.79-0.09 0. 0. 1.58-1.0 15 Imidazole--acetic acid -0.80 0.88 1.0 0.1.9-0.7 157 γ-butyrobetaine -0.80-1.00 0.00-0. 1.7 0.7 158 -Acetamidobutanoic acid -0.8 0. -0.8-0.00.9 1.79 159 Formylanthranilic acid -0.8-0.7-0. 0. 1.87-0.5 10 trans-glutaconic acid -0.8 0.70 0.7-0.70-0.9 1.5 11 Adenosine -0.8-0.0. 1.5 1.07-0.7 1 Shikimic acid -0.87-0.58 0.09 0.55 1.8-0.88 1 Glutathione (GSSG)_divalent -0.88-0.77-0.5 0.58 1.75-0. 1 ADMA -0.89-0.5-0.09 1.9 1.07-0.8 15 Digalacturonic acid -0.89 0.1-0.8 1.9-1.0 0.8 1 Nω-Methylarginine -0.89-0.1 0.1 1.70.50-0.78 17 CMP -0.91 1.5 1.08-0. -0.8-0.71 18 Cysteine glutathione disulfide -0.9 0.00 0.1 0.9 1.1.17 19 Ala-Ala -0.95-0.1-0.5 1.88-0.8 0.1 170 Gly -0.95-0.1-0.0 0.9 1.80.7 171 Methionine sulfoxide -0.9-0. -0.0 0.8 1.1-0. 17 Imidazolelactic acid -0.98 0.1 0.95 1.0.78-0.8 17 Ser -0.98 0.7 0.8-0.71-0.58 1.7 17 Isethionic acid -1.00.50-0.1 0.8-0.5 1.81 175 Gluconolactone -1.00. 0.10 1.5 0.91-1.1 17 Suberic acid -1.00.99-0.1 1. 0.5 0.1 177 '-Dephospho CoA -1.0-0. 0.00 1.7 0.51-0.5 178 XC0017 (Unknown) -1.0-0.9-0.1 0.91 1.50-0.8 179 N5-Ethylglutamine -1.0-0.7 0.78 1.9 0.1-0.77 180 Succinic acid -1.05-0. 0. 1.05 0.9-1.1 181 -ydroxyvaleric acid -1.05 1.18 0.7 0.8-0.80.8 18 N,N,N-Trimethyllysine -1.05-0.8-0.0 1.71 0. -0.08 18 Gluconic acid -1.0-0.1 0.17 1.1 0.79-1.09 18 Guanine -1.0 0.97-0.1 1.0-0.1-0.95 185 Arg-Glu -1.07-0.5-0.0 1.81-0.5 0.7 18 Glycerophosphocholine -1.11-0.5 0.5-0. 1.71-0.17 187 Allantoic acid -1.10.87 0.0 0.7-0.9 1.5 188 Isovalerylcarnitine -1.1-0.0 0.9 1.1 0.5-1.1 189 Isobutyrylcarnitine -1.15 0. 1.18 0. 0.79-1. 190 N-Acetyllysine -1.15-0. 0.0 1.8-0.8-0.8 191 O-Acetylcarnitine -1.17-0.88-0. 1.0 1. 0. 19 Tyr -1.18-0.9-0.7 0.5 0.95 1.8 19 Sorbitol -phosphate -1.19-0.18-0. 0.7-0.5 1.7 19 XC005 (Unknown) -1.0-0.1 0.1 0.7 1. -0.89 195 Serotonin -1.5-0. 0.09 1.75 0.19-0.7 19 XC01 (Unknown) -1.8-0.1-0.0 1.08 1. -0.7 197 Guanosine -1.9 0.08 0.1 1.50 0.8-0.9 198 Glycerol -1.5 0.0 0.7 0.9 1.7-0.98 199 Trimethylamine N-oxide -1.7 0.0.98 1.8 0.15 0.1 00 N-Acetylglucosamine 1-phosphate -1.0-0.1 0.1 0.8 1. -0.71 01 Thr -1.1-0.1 0.5 0.15 1.9-0. 0 Asn -1.0.89-0.1 0. 0.9 0.9 0 Gly-Asp -1.5-0.1-0.08 1.7 0.09 0.08 0 NAD+ -1. -0.0.0-0.05 1.1 0.9 05 Imidazole--methanol -1.5-0.5-0. 1. 0. 0.7 0 Phthalic acid -1.58 0.5 1.1 0. -0.57 0.1 07 NADP+ -1.0-0.8 0.57 0.88 0.99-0. 08 Glyceric acid -1.0-0.05 0. 1.0 0.91-0.5 09 N-Carbamylglutamic acid -1.1 0.1 0.1 1.08-0.7 0.8 10 cis-aconitic acid -1.8-0.0 0.5-0.5 1.19 0.9 11 Carnosine -1.81-0.19 0.1 1.18 0.1 0.5 1 Pyruvic acid -1.87 0.88 0.11 0.0 0.81-0.1
Supplementary Table S. Probe Names of Genes Represented in the Custom Microarray, EC Number and the KEGG Orthology (KO) ID of the Corresponding Protein Phenylalanine, Tyrosine and Tryptophan Biosynthesis Pathway (KEGG Map0000) KEGG ORTOLOGY EC number Probe Name (KO) ID 1..1.1 CUST_178_PI507 K0011...18 CUST_90_PI507 K007 CUST_81_PI507.5.1.5 CUST_1055_PI507 K01 CUST_158_PI507..1.1 CUST_71_PI507 K15..1.9 CUST_11589_PI507 K00817..1.51 CUST_91_PI507 K0518...5 CUST_990_PI507 K017 5..1. CUST_7_PI507 K1501 5..99.5 CUST_855_PI507 K01850
Glycolysis/Guconeogenesis Pathway (KEGG Map00010) EC number Probe Name 1.1.1.7 CUST_1058_PI507 K0001 CUST_1059_PI507 1..1.1 K001 CUST_1058_PI507 CUST_10900_PI507 CUST_10901_PI507 CUST_01_PI507 1..1. CUST_5_PI507 K0018 CUST_110_PI507 CUST_10890_PI507 CUST_50_PI507 1.8.1. CUST_11109_PI507 K008..1.1 CUST_70_PI507 K007.7.1.1 CUST_719_PI507 CUST_75_PI507 K008.7.1.11 CUST_970_PI507 K00850.7.1.0 CUST_597_PI507 K0087.7.. CUST_11_PI507 K0097.1..11 CUST_8_PI507 K081.1.1.1 CUST_88_PI507 K0158.1.1.9 CUST PI507 K0110.1..1 CUST_889_PI507 K01..1.11 CUST_18_PI507 K0189 5.1..15 CUST_80_PI507 K0179 5.1.. CUST_50_PI507 CUST_111_PI507 K01785 5..1.1 CUST_11091_PI507 K0180 5..1.9 CUST_01_PI507 K01810 5... CUST_1_PI507 CUST_79_PI507 K0185..1.1 CUST_110_PI507 K01895 KEGG ORTOLOGY (KO) ID
Pentose Phosphate Pathway (KEGG map0000) EC number Probe Name CUST_95_PI507 1.1.1. K000 CUST_110_PI507 1.1.1.9 CUST_77_PI507 K000..1.1 CUST_119_PI507 K0015 CUST_817_PI507..1. CUST_119_PI507 K001 CUST_105_PI507.7.1.11 CUST_970_PI507 K00850.7.1.1 CUST_00_PI507 K00851.7.1.15 CUST_55_PI507 K0085 CUST_501_PI507.7..1 K0098 CUST_110_PI507 CUST_1180_PI507.1.1.17 CUST_11809_PI507 K0105 CUST_710_PI507.1.1.1 CUST_75_PI507 K01057.1..11 CUST_8_PI507 K081 5.1..1 CUST_595_PI507 K0178 5..1. CUST_10980_PI507 K01807 5..1.9 CUST_01_PI507 K01810 CUST_1_PI507 5... K0185 CUST_79_PI507 KEGG ORTOLOGY (KO) ID
Supplementary Table S. Forward and Reverse Primers Synthesized for Quantitative PCR Analysis EC / Protein ID Forward Primer Reverse Primer EC.7.1.11 / 177 5'-TCA GCA ATA ACG TCC CGA TG-' 5'-TTG CTT GAT CGC ATC ACA GG-' EC1.1.1.9 / 10889 5'-ACG TTC AAG GAA CCG TTT GG-' 5'-ACC TGC AGC AAA TGG TTC TG-' EC.5.1.5 / 10881 5'-ACA CAT CCA AGA TGC GTT CG-' 5'-AAT TGC CAT GGG AGC AAT CG-' EC.5.1.5 / 108 5'-TTC TGA GAT CGA ACG CTT GC-' 5'-TTT CAT GAG TGC AGG CAT CG-' EC.5.1.5 / 10910 5'-ACG CCA AAC TCC TCA AAC AG-' 5'-TTG TTC GGG GCT TTT CGA AG-'
Supplementary Table S. Peptides Synthesized for Preparation of Polyclonal Primary Antibodies against the Target Proteins Target protein EC / Protein ID AMW a / kda Synthesized peptide phosphofructokinase (PFK) EC.7.1.11 / 177 9. CDNPGTTNYGEGTGQDTE glucose--phosphate dehydrogenase (GPD) EC1.1.1.9 / 10889 5.5 SDSGTIPSMETSDEC -deoxy-d-arabinoheptulosonate 7-phosphate (DAP) synthase -deoxy-d-arabinoheptulosonate 7-phosphate (DAP) synthase -deoxy-d-arabinoheptulosonate 7-phosphate (DAP) synthase a Average molecular weight EC.5.1.5 / 10881.9 MGKPLVPDFSDDEKVC EC.5.1.5 / 108 5.1 CEQNNLLYSELGGRERKP EC.5.1.5 / 10910 0.7 CRQGVRGRRELLKKQARP