Preparation of polystyrene magnetic microspheres for liquid chips by dispersion polymerization

Transcript

1 Vol. 24 No. 4 Chemical Research and Application Apr * SEM μm PDI Fe 3 O 4 FT-IR TG Fe 3 O 4 Fe 3 O 4 O A Preparation of polystyrene magnetic microspheres for liquid chips by dispersion polymerization NIU Ning-ning ZHOU Lei-ji * LIU Guo-an JIANG Shu-juan Department of Chemistry College of Chemistry and Chemical Engineering Xiamen University Xiamen Fujian China Abstract We present an approach of synthesizing magnetic polystyrene complex microspheres by a facile dispersion polymerization. Monodisperse magnetic polystyrene microspheres appropriate to construct liquid chip carriers were prepared via dispersion polymerization by dispersing pre-treated acrylic acid-based magnetic fluid in styrene. The effects of synthetic conditions such as pretreatment time of acrylic acid-based magnetic fluid feeding sequence and amount of styrene monomer on particles conformation and their size distribution have been investigated and parameters were further optimized accordingly. SEM results indicate that the magnetic microspheres have an average diameter of μm with excellent monodispersity PDI and smooth and compact surfaces. Magnetization curve of Fe 3 O 4 nanoparticles was obtained using superconducting quantum magnetometer. Chemical structures and content of magnetic Fe 3 O 4 of the microspheres were characterized and analyzed using FT-IR and TGA methods. Key words Fe 3 O 4 polystyrene magnetic microsphere dispersion polymerization J ljzhou@ xmu. edu. cn

2 AA MRI 11 P St-AA 14 FT-IR TGA SEM TEM 1 5 μm 1. 1 Fe acac 3 98% aladdin AR 80-90% AR St CR AA CR PVP K 30 AIBN AR 15 St N- N-isopropylacrylamide NIPAM PSNN Fe 3 O 4 Fe acac mmol Fe 5-35 μm Liu et acac ml 1. 5 ml 9 ml al St /NH 2 -PEO-AA St /PEO-VB P St-MPEO 5-80μm Fe 3 O 4 μm Zeng PEG Fe 3 O 4 St AA PEG 250 ml 1. 0 g PVP 2 μm Zhang et ml V EtOH /V H2 O = g al 19 72% P Fe 3 O 4 1 ml AA 25 St GMA 1 h 0. 1 g AIBN μm 10 g St PVP 10 min 70 8 h N X X'pert PRO Fe 3 O 4 Fe 3 O 4

3 4 505 JEM-2100 Fe 3 O 4 Hitachi S min AA PDI MPMS-XL-7 1 Quantum Design Fe 3 O 4 Table 1 Effect of pretreatment time on size and size Nicolet distribution of magnetic microspheres Avatar 330 Min D n PDI SDT Q600 TA Fe 3 O AA Fe 3 O 4 AA AA Fe 3 O 4 AIBN AA AIBN AIBN Fe 3 O 4 PVP 70 PVP 0. 5 g 1. 4 g min PDI 1 30 min min 1 h min 10 g 8 g 6 g 2 6

4 g St cm -1 -CH 2 - C-H 1705 cm -1 C = O 10 g 8 g 8 g μm PDI Table 2 2 Effect of monomer on size and size distribution of magnetic microspheres g D n PDI a. Fe 3 O 4 b XRD Fig. 2 FT-IR spectra for analysis of chemical structures. 1 Fe 3 O 4 X a. Fe 3 O 4 nanoparticles b. magnetic polystyrene microspheres 2θ Fe 3 O 4 Fe 3 O P St-AA 2θ nm 340 ~ Fe 2 O 3 Fe 3 O 4 Fe 2 O 3 Fe 2 O 3 Fe 3 O % 1 Fig. 1 Fe 3 O 4 XRD X-ray diffraction patterns of the iron oxide nanoparticles P St-AA 2a 580 cm -1 Fe 3 O 4 Fe-O 3 2b 580 cm -1 Fig. 3 Fe-O 3082 ~ 3024 cm -1 C-H 1601 ~ 1450 cm cm -1 C-H 10 /min TGA analysis of magnetic polystyrene microspheres. The curve was obtained at a heating rate of 10 /min under a oxygen atmosphere

5 b 5a Fe 3 O 4 TEM 4 10 min Fe 3 O μm PDI Fe 3 O 4 5c 5. 4 nm 21 Fe 3 O 4 35 nm P St-AA 20s 5 SEM Fig. 4 4 Fe 3 O 4 a. TEM b. c. Characterization of Fe 3 O 4 nanopariticles. a. TEM photograph b. size distribution c. magnetization curve 3 5 a b. SEM c. Fig. 5 Conformation and magnetic response characteristic of magnetic polystyrene microspheres. a and b. SEM photograph c. magnetic response characteristic P St-AA 5. 4 nm Fe 3 O 4 Fe 3 O 4 AA

6 Obata K Tajima H Yohda M et al. Recent developments in laboratory automation using magnetic particles for genome analysis J. Pharmacogenomics Goldner H. MATERIALS PRODUCTS J. R&D Mag Safarik I Safarikova M. Magnetic techniques for the isolation and purification of proteins and peptides J. Biomagn Res. Technol Pamme N. Magnetism and microfluidics J. Lab Chip Prestvik W S Berge A Mork P C et al. Preparation and application of monosized magnetic particles in selective cell separation Scientific and clinical applications of magnetic carriers 1997 C. 6 Safar I K I Safar I Kova M. Use of magnetic techniques for the isolation of cells J. J. Chromatogr. B Rittich B Spanova A Salek P et al. Separation of PCRready DNA from dairy products using magnetic hydrophilic microspheres and poly ethylene glycol -NaCl water solutions J. J. Magn. Magn. Mater Weizmann Y Patolsky F Katz E et al. Amplified DNA Sensing and Immunosensing by the Rotation of Functional Magnetic Particles J. J. Am. Chem. Soc Liu Z Zhang L Yang H et al. Magnetic microbead-based enzyme-linked immunoassay for detection of Schistosoma japonicum antibody in human serum J. Anal. Biochem Gupta A K Gupta M. Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications J. Biomaterials Yezhelyev M V Gao X Xing Y et al. Emerging use of nanoparticles in diagnosis and treatment of breast cancer J. Lancet Oncol Rorrer G L Hsien T Y Way J D. Synthesis of porousmagnetic chitosan beads for removal of cadmium ions from wastewater J. Ind. Eng. Chem. Res Guo S S Zuo C C Huang W H et al. Response of superparamagnetic beads in microfluidic devices with integrated magnetic micro-columns J. Microelectron. Eng Zhou L Wang K Tan W et al. Quantitative Intracellular Molecular Profiling Using a One-Dimensional Flow System J. Anal. Chem J Liu X Y Ding X B Zheng Z H et al. Synthesis of novel magnetic polymer microspheres with amphiphilic structure J. J. Appl. Polym. Sci Liu X Y Ding X B Zheng Z H et al. Synthesis of amphiphilic magnetic microspheres by dispersion copolymerization of styrene and poly ethylene oxide macromonomer J. Polym. Int Zeng H M Lai Q Y Liu X Q et al. Factors influencing magnetic polymer microspheres prepared by dispersion polymerization J. J. Appl. Polym. Sci Zhang J Yu D M Chen W et al. Preparation of poly styrene-glucidylmethacrylate /Fe3O4 composite microspheres with high magnetite contents J. J. Magn. Magn. Mater J Dunlop D J. Magnetite Behavior near the Single-Domain Threshold J. Science