Cellular Physiology and Biochemistry

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1 Original Paper 2016 The Author(s) Published The Author(s) by S. Karger AG, Basel Published online: November 25, Published by S. Karger AG, Basel Accepted: September 28, 2016 This article is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND) ( Usage and distribution a Fei Li b Jing Shi c Songlin Li d e Youhong Jiang a a b Department of Internal c d e Background/Aims: Methods: Biopsies model establishment and macrophage isolation were performed on Sprague Dawley (SD) rats. mir-146a inhibitors, and TRAF6 sirnas. Serum creatine phosphokinase (S-CK) expression was assessed using double antibody sandwich enzyme-linked immunosorbent assay (ELISA) assay, and immunohistochemistry assay was performed to analyze CD163 expression in muscle samples. Furthermore, we used transwell assay to test cell migration; RT-PCR and western blot Results: healthy controls and were down-regulated after the conventional treatment. Treatment with whereas mir-146a inhibitors exerted the opposite effects. The effects of mir-146a inhibitors were suppressed by treatment with TRAF6 sirna. In addition, the luciferase reporter assay validated the targeting relationship between mir-146a and TRAF6. Conclusions: 2016 The Author(s) Published by S. Karger AG, Basel Youhong Jiang

2 Published online: November 25, Polymyositis/dermatomyositis (PM/DM), which is characterized by meristic proximal muscle weakness and the elevation of muscle enzyme level, is an unusual idiopathic evidence-based theory to determine the appropriate treatment for PM/DM, and physician detected in the sera of patients with various diseases, it has been linked to the development of Clinical samples

3 488 - Animal model th th Tests for serum creatine phosphokinase (S-CK), IL-17 and ICAM-1 Cell culture adherence

4 489 incubator, Immunohistochemistry Luciferase activity assay Transwell assay RNA isolation and RT-PCR

Expression of mir-146a, TRAF6, IL-17, and ICAM-1 in the muscle samples of patients and

5 490 time-polymerase chain reaction Western blotting Statistical analysis t P Results Clinical assessments, muscle histopathology and effects of conventional hormone treatment Expression of mir-146a, TRAF6, IL-17, and ICAM-1 in the muscle samples of patients and healthy controls patients both prior to treatment and after treatment, and after the conventional hormone P <

6 491 ** P, # P ## P (stained in brown) in model rats from the control P < Expression of CD-163, s-ck, mir-146a, TRAF6, IL-17, and ICAM-1 in the muscle samples of polymyositis model rats

7 492 - ** P # P ## P P * P P P

8 493 - ** P ## P P P P MiR-146a suppresses TRAF6 expression by binding to the 3 UTR activity (P MiR-146a/TRAF6 regulates macrophage migration P

9 494 - * P ** P < ## P P P < molecules (P < P <

10 495

11 496

12 Published online: November 25,

13 498 and in situ expression of cellular adhesion molecules implicated in the cohesion of endothelial cells in

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