1 Vol. 16 No ELECTROCHEMISTRY Feb mmol /L dimethylamino propyl -3-eth- 5 ylcarbo-diimide hydrochloride 99% EDC 6-7
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1 16 1 Vol 16 No ELECTROCHEMISTRY Feb L- /GC * L L- L- O646 A Chloroperoxidase CPO 1 1 CPO Sigma L- Sigma 50 mmol /L ph = mmol /L P dimethylamino propyl -3-eth- 5 ylcarbo-diimide hydrochloride 99% EDC CPO Heal Force CPO 50 mmol /L CPO PBS KH 2 PO 4 -Na 2 HPO 4 ph 0 1 mol /L KCl 1 2 L- CHI Rusling GC = 3 mm L- Poly-L-lysine PLL L- GC CPO PLL / 1 3 GC GC 6# 0 3 μm * Tel xqwu@ shnu edu cn QA14044
2 μm α-al 2 O 3 CPO Fe Ⅲ / Fe Ⅱ V GC 10 mmol /L ph = 9 0 L ~ 2 5 V CPO PLL /GC 100 mv /s PLL CPO PLL PLL /GC 1 μl mmol /L CPO 4 μl 24 mmol /L EDC PLL /GC CPO- PLL /GC L V Ep 22 mv 100 mv /s 1 L- L- 8 L- 2 GC a PLL /GC b CPO-PLL /GC c PLL 8 Fig 2 Cyclic voltammograms of bare GC a PLL /GC b 8 and CPO-PLL /GC electrode c in N 2 -saturated 50 mmol /L PBS ph = 2 75 containing 0 1 mol /L KCl scan rate is 100 mv /s 1 Fig 1 L Cyclic voltammograms of electropolymerization for 10 mmol /L L-lysine ph = 9 0 at GC electrode scan rate is 100 mv /s 3 CPO PLL /GC ~ 1 5 V /s 17 Ipa μa = 0 015v mv /s R 1 = Ipc μa = v mv / s R 2 = 2 3 ph 4 CPO-PLL/GC ph 3 0 ~ PLL /GC CPO ph ph 2 ph 2 75 CPO Fe Ⅲ /Fe Ⅱ GC a PLL / GC b E 0 PLL /GC CPO c V ph E 0 V =
3 1 - L- /GC 67 3 CPO-PLL /GC A B Fig 3 Cyclic voltammograms of CPO-PLL /GC electrode in a N 2 -saturated 50 mmol /L PBS ph = 2 75 containing 0 1 mol /L KCl scan rate a ~ g /V s A and the peak current versus scan rate B 4 Fig 4 CPO-PLL /GC ph PBS A E 0 ~ ph B Cyclic voltammograms of CPO-PLL /GC in different ph a ~ f PBS containing 0 1 mol /L KCl A and the of formal potential E 0 versus ph B scan rate 100 mv /s ph R = mv /ph 57 6 mv /ph CPO-PLL /GC 5 CPO-PLL /GC 100 mv /s 100 CPO-PLL /GC 40 d 5 CPO-PLL /GC N CPO mol /L KCl 50 mmol /L PBS 2 5 CPO-PLL /GC 100 Fig 5 The successive 100 cyclic voltammograms of CPO- PLL /GC electrode in a N 2 -saturated 50 mmol /L 6 GC a PLL /GC b CPO-PLL /GC PBS ph = 2 75 containing 0 1 mol /L KCl c scan rate 100 mv /s
4 GC V al C fumago chloroperoxidase is also a dehaloperoxidase oxidative dehalogenation of halophenols J J Am PLL /GC V Chem Soc CPO V 4 John H Dawson Masanori Sono Cytochrome P-450 and GC PLL /GC chloroperoxidase thiolate-ligated heme enzymes Spectroscopic determination of their active site structures and 620 mv 350 mv mechanistic implications of thiolate ligation J Chem CPO Rev Fig 6 chloroperoxidase at GC electrode modified with SWNTs GC a PLL /GC b CPO-PLL /GC c in Chinese J Electrochemistry mmol /L PBS ph = 2 75 Cyclic voltammograms of the bare GC a PLL /GC b and CPO-PLL /GC c electrodes in O 2 -saturated 50 mmol /L PBS ph = 2 75 scan rate 100 mv /s 3 PLL CPO xin Cyclic voltammetric determination of dopamine in drug using poly L-lysine modified electrode J Chinese Journal of Analytical Chemistry CPO CPO-PLL /GC Zhao Yan-xia Ma Xin-ying Wang CPO-PLL /GC Qi et al Electrocatalytic oxidation to norepinephrine at a poly L-lysine modified electrode J Chinese Journal of Analysis Laboratory References 1 Cao Shu-yu Liu Jun-hong Biotechnology progress on applications of peroxidase J Journal of Chemical Industry & Engineering Lowell P Hager David R Morris Frederick S Brown et al Chloroperoxidase Ⅱ Utilization of halogen anions J Biological Chem Robert L Osborne Gregory M Raner Lowell P Hager et 5 Lu Zhong-qing Hou Wen-jing Wu Xia-qin et al Electrochemistry of self-assembled ultra-thin films composed of chloroperoxidase and polyions J Chem Lett Zhang Hong-xia Wu Xia-qin Hou Wen-jing 瞡 et al Catalytic chlorination of MCD using chloroperoxidase coated single-wall carbon nanotubes films on GC electrodes J Chemical J of Chinese University Zhang Hong-xia Wu Xia-qin Hou Wen-jing 瞡 et al Direct electrochemistry of Yang Liu Wu Xia-qin Wang Rong et al Ionic liquid modified GC electrode for the direct electrochemistry of chloroperoxidase J Chinese Chemical Letters Sun Deng-ming Ma Wei Zhang Zhen Wang Xiu-wen Yu Fen Zhao Zhijie et al Electrochemical behavior of acetaminophen at poly L-lysine-modified electrode and its detection J Chemistry & Bioengineering Peterson M Guto Challa V Kumar James F Rusling Thermostable peroxidase-polylysine films for biocatalysis at 90 J J Phys Chem B
5 1 - L- /GC Abhay Vaze Michael Parizo James F Rusling Enhanc- 13 ed rates of electrolytic styrene epoxidation catalyzed by cross-linked myoglobin-poly L-lysine films in bicontinuous microemulsions J Langmuir Peterson M Guto James F Rusling Enzyme-like kinetics of ferryloxy myoglobin formation in films on electrodes in microemulsions J J Phys Chem B Abhay Vaze James F Rusling Microemulsion-controll- 15 ed reaction sites in biocatalytic films for electrochemical reduction of vicinal dibromides J Langmuir Zhang Zhe Salem Chouchane Richard S Magliozzo et al Direct voltammetry and catalysis with mycobacterium tuberculosis catalase-peroxidase peroxidases and catalase in lipid films J Anal Chem Alan P Brown Fred C Anson Electron transfer kinetics with both reactant and product attached to the electrode surface J Journal of Electroanalytical Chemistry The Electrochemical Characteristics of CPO-PLL / GC Electrode ZHAO Wen WU Xia-qin * LU Zhong-qing HOU Wen-jing LI He-xing Life and Environment Science College Shanghai Normal University Shanghai China Abstract Poly-L-lysine PLL was electropolymerized onto the surface of glassy carbon GC electrode 1-3-dimethylamino propyl -3-ethylcarbodiimide hydrochloride EDC was used as a Cross-linking agent to immobilized Chloroperoxidase CPO onto the PLL /GC electrode A pair of well-defined redox peaks was observed at CPO-PLL /GC electrode which clearly suggested that PLL can promote the direct electron transfer between the CPO and the GC electrodes The cyclic voltammetry results also showed that the electron transfer at CPO-PLL / GC is a surface adsorption-controlled quasi-reversible process which is accompanied with proton transfer The CPO-PLL / GC electrode exhibited a good electrochemical stability and excellently electrocatalytic activity for electrochemical reduction of O 2 Key words chloroperoxidase poly-l-lysine electropolymerization direct electrochemistry electrocatalysis modified electrode
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