Advances in the study of to0in in halobios

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Ὀλυσσεύς Λούλης
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1 11 3 Vol.11 No J0URNAL 0F SHANGHAI FISHERIES UNIVERSITY Sep. 2002!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! Advances in the study of to0in in halobios WANG Yan ZH0U Pei-gen QI Xiao-yu College of Food Science Shanghai Fisheries University Shanghai China Key words toxin tetrodotoxin Conotoxin anthoplerin toxin S917 A Tetrodotoxin TTX Na + Na + TTX TTX Na + Na TTX TTX TTX Tel ywang2@ shfu. edu. Cn

2 TTX 2 TTX TTX TTX 6 7 TTX TTX Na + Fig. 1 The structure formula of Tetrodotoxin TTX TTX C-4 C-9 C-10 ph 1994 TTX TTX 3 TTX 2 Conotoxin CTX 10 ~ 30 α #! $ α-ctx AChR #-CTX Ca 2 +! -CTX Na + $-CTX Na + 1 ~ 2pmol 2.1 CTX α #-CTX NMR CD X !-CT Gehrmann 4 α-ctx GI NMR α-ctx GI Maslennikon 5 NMR α-ctx GI α-ctx GI

3 285 78% 22% Rogers 6 α-ctx IMI 2 loop Hu 7 X- α-ctx PnIB α-ctxpnia GI 2 PnIB 2 P2 1 2 1 2 1 Shon 8 HN - MR α-ctx MII 2 2 α-ctx #-CTX 14 AshCom 9 Fig.

3 % 22% Rogers 6 α-ctx IMI 2 loop Hu 7 X- α-ctx PnIB α-ctxpnia GI 2 PnIB 2 P Shon 8 HN - MR α-ctx MII 2 2 α-ctx #-CTX 14 AshCom 9 Fig. 2 Amino acid sequence ofα-ctx 6#-CTX α-ctx GI FGI N- Spin-Column FGI nachr K D 士 8 2nmol / L Qniram 10 α- CTXIMI Asp5 Arg !-CT Kim 11 #-CTX GVIA CD #-CTX MVIIA #-CTX MVIIC CD GVIA 2 CD 200nm Cotton CD CD Hirata 12 #-CTX GVIA MVIIA MVIIC Ca 2 + N Ca 2 + ATP Marian PriCe-Carter 13 #-CTX MVIIA Ca 2 + Lew 14 #-CTX GVIA Lys2 Tyr13 Ala 2 Argl7 Tyr22 Lys24 Ala < 100 GVIA N Ca 2 + Marian PriCe-Carter 15 #-CTX MVIIA MVIIA - Gly Gly 80% Pro-#-CTX MVIIA MVIIA - Gly MVIIA 10 Pro-#-MVIIA 100 ZiConotide #-CTX N ZiConotide 25 3 Fig.3 The space structure of Conotoxin by NMR I 3

4 Anthoplerin toxin AP ATX Fig. 4 4 The structure formula of Anthoplerin toxin 3.1 PDMS 士 0.15 C 129 H 223 N NaI0 4 4 Kishi 16 8 C 1 -C 7 C 8 -C 22 C 23 -C 37 C 38 -C 51 C 52 -C 75 C 76 -C 84 C 85 -C 98 C 99 -C AP-A AP-B AP-A Na + AP-B Na + Kelso 17

5 3 287 Anthopleura xanthogrammica 6 5 Anmoniaa sulcata 47 I 49 RT-4B NIE-II5 PCR Na + RT-4B NIE-II5 K nmol / L 1354nmol / L 49 Na + K nmol / L 108nmol / L 4 Jellyfish 18 Cephalotoxin ~ Tripheustes gratilla ph4.3 ~ ~ 47.5 Lytechinus variegatus 0. 25mol / L Na0H l ~ 2h Lacticaudza semifisciate latictoxin I ~ V 20 90% dolastoxin Dolabella auricularia 1 ~ 9 3 Pro-Leu-Val- Gln - Thz- Gly -The 3 Gln -Thz Gln -ThZ P 388 B 15 3 microcystin cyanobacteria Microcyatis aeruginosa 50 MCdermott 21 -LR 30min 0.8! M 100! M DNA 5

6 Baden D G. Marine food-borne dinoflagellate toxins C. Review Cytology Kao C Y. StruCture-aCtivity relations of tetrodotoxin saxitoxin and analogues C. Annals of the New York ACademy of science Tambyah P A. Central-nervous-system effects of tetrodotoxin poisoning M. LanCet Gehrmann J. StruCture determination of the three disulfide bond isomers of α-conotoxin GI a model for the role of disulfide bond in structural stability J. J Mol Biol Maslennikov I V. Two distinct structures ofα-conotoxin GI in aqucous solution J. Eur J BioChem Rogers J P. NMR solution structure ofα-conotoxin ImI and Comparison to other Conotoxins specific for neuronal nicotinic acetyleholine receptors J. BioChemistry Shu-Hong Hu. Crystal structure at a resolution ofα-conotoxin PnIB Comparison withα-conotoxin PnIA and GI J. BioChemistry Ki-Joon Shon. Three-dimensional solution structure of α-conotoxin MI anα3"2 neuronal nicotinic acetylcholine receptor-target edligand J. BioChemistry AshCom J D. CharaCterization ofα-conotoxin in teractions with nicotinic acetylcholine receptor and monoclonal antibodies J. Chem J Quiram P A. CharaCterization ofα-conotoxin ImI essential for binding to neuronalα7 receptors J. The Journal of BiologiCal Chemistry Kim J. CirCular dichroism spectra of CalCium Channal antagonist #-Conotoxin J. BioChemiCal and biophysical Hirata H. #-Conotoxins block neurotransmission in therat vas deferences by binding to gifferent presynaptic sites on the N-type Ca 2 + BioChemistry PriCe-Carter M. Roles of individual disulfide bonds in the stability and folding of an#-conotoxin J. BioChemistry Lew M J. StruCture-funCtion relationships of #-Conotoxin GVIA J. The Journal of BiologiCal Chemistry Channel J. 15 PriCe-Carter M. Folding of#-conotoxin influence of precursor sequences and protein disulfide isomerase J. BioChemistry Kishi R E. SCienCe M Kelso G J. IdentifiCation and CharaCterization of novel sodium Channel toxins from the sea anemone Anthopleura xanthogrammica M. Toxin RiChard Benzinger. A specific interaction between the CardiaC sodium Channel and site-3 toxin anthopleurin B J. The Journal of BiologiCal Chemistry J A. C MCdermott C M. The CyanobaCterial toxin microcystin-lr CaninduCe apoptosis in a variety of Cell types M. Toxin !!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! BSA A

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