Cutaneous Blood Flow Increased by CO, Water Foot Bathing Visualized with Laser Doppler Imaging

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1 12, +, , ,2 +., 0 Cutaneous Blood Flow Increased by CO, Water Foot Bathing Visualized with Laser Doppler Imaging Shoichi OKOUCHI +, Daisuke TAKEZAKI +, Hideyuki OHNAMI +, Yuhki SHYUTOH + Shigeo IKEDA +, Yukio KENJO,, and Yuhkoh AGISHI - + Faculty of Engineering, Hosei University, Integral Co., LTD - Research Institute for Health Resort Medicine Abstract Artificial CO, spring water (CO, concentration of +,*** ppm) and tap water were prepared in the temperature range of -. to., for foot bathing. Laser doppler imaging of cutaneous blood flow in the foot immersed for foot bathing made it possible to visualize the two-dimensional spatial variation of the perfusion while being not only noninvasive but also non-touch. Therefore, it reduced the risk of influence on skin perfusion mechanically, being di#erent from the method used so far. This method revealed that the cutaneous blood flows by CO, water foot bathing were increased from nearly two to four times in comparison with those by tap water foot bathing. In Japan, natural CO, spring waters are rare. However, we proposed that development of a co-generation system with hydrogen cell evolved exothermic heat, and hydrogen and carbon dioxide gases makes it possible to prepare and perform easily artificial CO, bathing therapy, with a reductive characteristic analogous to natural spring water, at home and elsewhere. Key words : CO, thermal spring water, Laser doppler imaging, Cutaneous blood flow, Visualization, Fuel cell. : +

2 /,,**,, ; Schmid, +332 ; Hartmann et al., +332 ; +33/ ; a, +32.b ; +3-0 ; +3-/ +,*** ppm +332,*** ;,*** ;,**, Fullerton et al., +33/ ; Wardell et al., +33-,,, -.., CO, +,*** ppm,, PIM Lisca 01* nm, 0,+,.,,/ 0* ,-** ppm ,*** ppm,,,

3 14 - +*. - Fig.. Cutaneous blood flows of foot by CO, bathing V (CO, concentration of +,*** ppm) and tap water bathing for a,- years old female. -..,, / -. 0 Fig. 0 Cutaneous blood flows of foot by CO, bathing - (CO, concentration of +,*** ppm) and tap water bathing. at -. for a,- years old female. Gschwandtner et al. ;+330 -, +33,,**. / 1,*

4 /,,**,, 15 Fig. 1 Schematic diagram of co-generation system of hydrogen cell with evolving exothermic heat and hydrogen and carbon dioxide gases. 1 1 Fig. 3 Relationship between ORP and ph in artificial CO, spring waters dissolved CO, and CO, with ORP, vol H, using multi layered composite membrane ph hollow fiber at.*.,*** 2 ORP ph 3,.* ph 2

5 , +3-0 :,,30 -,/ : MHF + +1,*. +3-/ : +,+3,/-.,*** : - +/,*.,*** : ORP ph /* 3. +* : ORP ph.3 / :.2,3 -/. Gschwandtner, M.E., Koppensteiner, R., Maca, T., Minar, E., Schneider, B. and Schnurer, G. (+330) : Spotaneous laser doppler flux distribution in ischemic ulcers and the e#ect of prostaoids ; A crossover study comparing the acute action of prostaglandin E + and lloprost vs saline, /+,, , :. Schmid, K.L. : +332 : + / :

6 /,,**,, 17, / : /2 +,+ +,0. Hartmann, B., Pittler. M. and Drews, B. : +332 : CO, : + +* +0. Fullerton, A., Serup, J. (+33/) : Laser doppler image scanning for assessment of skin irritation., Elsner, P., Maibach HI (eds), Irritant Dermatitis. New Clinical and Experimental Aspects. Current Problems in Dermatology,,-, +/3 +02, S. Karger, Basel, Switzerland.,**, : +, +- C, +321 : No. 2,- -* : +.1 +,- +, :, 1 +-* +-0.,*** : / 2. Wardell, K., Jakobsson, A. and Nilsson G. E. (+33-) : Laser doppler perfusion imaging by dynamic light scattering, IEEE Trans. Biomed. Eng.,.*, -* * : 03 1-

7 ῌ 18 Fig. + CO, bubbles on human skin and human skin turned into red by natural CO, bathing (temperature : -.ῌ and CO, concentration : +,-** ppm) Fig., Laser doppler imaging of cutaneous blood flow of foot measured in air. Fig. - Laser doppler imaging of cutaneous blood flow of foot after CO, bathing (CO, concentration of +,*** ppm) and tap water bathing of ten minutes for a,- year old female.

8 /,,**,, 19 Fig. / Laser doppler imaging of cutaneous blood flow of foot by CO, bathing (CO, concentration of +,*** ppm) and tap water bathing at -. for a,- year old female. Fig. 2 Diagrammatic representation of relationships between ORPand ph in natural and artificial CO, spring water, in human skin, and in tap water.