Advances in Astaxanthin Production by

Μέγεθος: px

Εμφάνιση ξεκινά από τη σελίδα:

Download "Advances in Astaxanthin Production by"

ΣoφпїЅα Καλύβας
5 χρόνια πριν
Προβολές:

2007 7 July 2007 CHEMICAL INDUSTRY AND ENGINEERING 24 4 Vol. 24 No.

1 July 2007 CHEMICAL INDUSTRY AND ENGINEERING 24 4 Vol. 24 No. 4 : (2007) , (, ) :,,, : ; ; ; : TQ920 :A Advances in Astaxanthin Production by Phaffia Rhodozyma Fermentation SUN Nai2xia, ZHAO Xue2ming (School of Chemical Engineering and Technology, Tianjin University, Tianjin , China) Abstract :Astaxanthin is a high2value2additive, which has wide applications in food, medicine and cosmetics. Phaffia rhodozyma is one of the microorganisms which produce natural astaxanthin. In this paper, the research advances and methods in breeding and fermentation optimization of Phaffia rhodozyma are summarized. Furthermore, strategy was proposed to solve the problems in large2scale commercial production of astaxanthin. Key words : Phaffia rhodozyma ; astaxanthin ; breeding ; fermentation optimization (3,3 2 24,4 2 2, 2 ),, [1 ],, [2 ],,, 2000 FDA,, :,, (DCW) ( gπg),, [3 ],,,,Igene, Igene, (DCW) : : (NSFC ) ; 973 (2003CB716003) : ( ),,, :, : (022) ,E2mail edu. cn

2 mgπkg,, 1,,,, DNA , [3 ],,, [4 ], 60 Co 137 Cr Namkyu sun [5 ] 3 kgy, gπg gπg [6 ] 315 kgy (NTG),, Lewis [7 ] NTG NTG gπg 600 gπg [13 ] NTG Fang [8 ] NTG, 565 gπg 1516 gπg ( EMS), benomyl,, Calo [9 ] 232 %, gπg EMS ( 20 % 40 %) [3 ] 11113, 1) :,,,, (DPA) 2 Namthip [10 ] DPA, molπl molπl DPA, 475 gπg 914 gπg Sanpietro [11 ] DPA : molπl, 2, molπl,, 2 2, 2 C23 C24, molπl 2, Lewis [7 ] CBS672210, NTG 2 NTG2327, 3, gπg 2) : ( FPP), Johnson [12 ] A (HMG2CoA) ( ),, Compactin Lovatatin Lovatatin, gπg, % 3) : Schroeder [14,15 ],,, A [3 ] b2c1,,, 4 gπl 3 %, [16 ] O 3 Π 10 20

3 24 4 : 341 min 2terthienyl,,An [17 ],, (Quantitative Flow Cytometry) (Cell Sorting) , gπg 112,,, Prevatt [18 ] ( Trichoderma harzianium), Novozymes Novozyme234, 1 2 gπl [19 21 ] 1992,Chun [22 ], gπg, gπg,, ( < ) Santopictro [23 ] ( Cryptococcus laurentii) ( Saccharomyces cerevisiae),,, [19 ], [4 ], 9714 % [21 ] ,: [24 ], [25 ], Wang [26 ], Agrobacterium aurantiacum crt, ( IPP) Archaebacterium GGPP, 1125 mgπg,,, ,Verdoes [24 ], [27,28 ] crtyb, GGPP, 2, [24 ],ast 2, P450 [28 ], NADH Fe 2 +, 2, Adrio [29 ] LiAc, Hyg R Pleo R Km R, Km R G418,, PGH21,, ( DNA ) Πg 1997,Wery [30 ] PGH21 rdna, PGB2Ph9 rdna,, DNA ( < Πg) ppr2t (gpd), Km R

4 , Πg DNA,, PEG, DNA, [29 ] PEGΠ [30 ], (electroporation), DNA Rubinstein [31 ], LiCl, V 50 F 2 000, Πg DNA, Verdose [32 ] crtyb ( ), 2, crti ( ), 2,, : 211, ph Johnson [33 ] : 20 22, ph 510,,, 30 mmolπ(l h),, 2 Meyer [34 ] ph, Yamane [35 ] (CΠN), CΠN Crabtree, (45 gπl), [36 ] CΠN,, ;, 120 h, 30 gπl, 2116 mgπl 720 gπg Ramirez [37 ] ph,,, 811 mgπl, 92 % 212, Parajo [38 ], 0107 h - 1, 0147 gπg, mgπkg Harrd [39 ] 10 %, 3,,, Fang [8 ] NCHU2FS301 :, mgπl, = 1 1 ( ),, 213 Calo [9 ] 1 gπl, 300 % Flores2Cotera [40 ], molπl, 3,,,,,

5 24 4 : 343,, DNA, : [ 1 ] SHIMIDZU N, GOTO M, MIKI W. Carotenoids as singlet oxygen quenchers in marine organisms [ J ]. Science, 1996, 62 (1) : Fisheries [ 2 ] TANAKA T, MAKITO H, OHNISHI M, et al. Chemoprevention of rat oral carcinogenesis by naturally occurring xanthophylls, astaxanthin and canthaxanthin [J ]. Cancer Res, 1995, 55 : [ 3 ] JOHNSON E A, SCHROEDER W. Astaxanthin from the yeast Phaffia rhodozyma[j ]. Stud Mycol, 1995, 38 : [ 4 ]. [D ]. :,2001. [ 5 ] NAMKYU S, SEUNGHEE LEE, KYUNG B S. Characterization of a carotenoid2hyperproducing yeast mutant isolated by low2dose gamma irradiation [ J ]. Journal of Food Microbiology, 2004, 94 : [ 6 ],,,. International 60 Co [J ]., 2005,14 (3) : [ 7 ] LEWIS M J, RAGOT N, BERLANT M C, et al. Selection of astaxanthin2overproducing mutants of Phaffia rhodozyma with 2ionone [ J ]. Appl Environ Microbiol, 1990, 56 : [ 8 ] FANG T J, CHENG Y S. Improvement of astaxanthin production by Phaffia rhodozyma through mutation and optimization of culture conditions [ J ]. J Ferment Bioeng, 1993, 75 : [ 9 ] CALO P, MIGUEL T, VELAZQUEZJ B, et al. Mevalonic acid increases trans2astaxanthin and carotenoid biosynthesis in Phaffia rhodozyma[j ]. Biotechnology Letters, 1995, 17 (6) : [10 ] CHUMPOLKULWONG N, KAKUZONO T, NAGAI S, et al. Increased astaxanthin production by Phaffia rhodozyma mutants isolated as resistant to diphenylamine [ J ]. J Ferment Bioeng, 1997, 83 (5) : [11 ] SANPIETRO L M D, KULA M R. Studies of astaxanthin biosynthesis in Xanthophyllomyces dendrorhous ( Phaffia rhodozyma). Effect of inhibitors and low temperature [J ]. Yeast, 1998, 14 (11) : [12 ] GU W L, AN G H, JOHNSON E A. Ethanol increases carotenoid production in Phaffia rhodozyma[j ]. J Industrial Microbiology & Biotechnology, 1997, 19 (2) : [13 ]. [D ]. :,2004. [14 ] SCHROEDER W A, JOHNSON E A. Antioxidant role of carotenoids in Phaffia rhodozyma [ J ]. J Gen Microbiol, 1993, 139 (5) : [15 ] SCHROEDER W A, JOHNSON E A. Carotenoids protect Phaffia rhodozyma against singlet oxygen damage[j ]. J Ind Microbiol, 1995, 14 (6) : [16 ],,. NTG [J ].,2003,20 (5) : [17 ] AN G H, BIELICH J, JOHNSON E A, et al. Isolation and characterization of carotenoid hyperproducing mutants of yeast by low cytometry and cell sorting [ J ]. BioΠ Technology, 1991, 9 : [18 ] PREVATT W D, BARTLESVILLE O. Spheroplast fusions of Phaffia rhodozyma cells :US, A[ P] [19 ]. ( Phaffia rhodozyma) [D ]. :, [20 ]. [D]. :,1999. [21 ] CHUN S B, CHIN J E, AN G H, et al. Strain improvement of Phaffia rhodozyma by protoplast fusion[j ]. FEMS Microbiol Lett, 1992, 93 : [22 ] SANPIETRO L M D, SPENCER J F T, SPENCER D M, et al. Characterization of intergeneric hybrids obtained by protoplast fusion between Phaffia rhodozyma, Cryptoococcus laurentii and Saccharomyces cerevisiae [ J ]. Biotechnology Techniques, 1997, 11 (10) : [23 ] VERDOES J C, MISAWA N, VAN OOYEN A J J. Cloning and characterization of the astaxanthin biosynthetic gene encoding phytoene desaturase of Xanthophyllomyces dendrorhous[j ]. Biotechnol Bioeng, 1999, 63 : [24 ] KAJ IVARA S, FRASER P D, MISAWA N, et al. Expression of an exogenous isopentenyl diphosphate isomerase gene enhances isoprenoid biosynthesis in Escherichia coli[j ]. Biochem J, 1997, 324 : [25 ] WANG CH W, OH MIN2KYU, LIAO J C. Engineering isoprenoid pathway enhances astaxanthin production in Escherichia coli [ J ]. Biotechnology & Bioengineering, 1999, 62 (2) : [26 ] VERDOES J C, KRUBASIK P, VAN OOYEN A J J. Isolation and functional characterization of a novel type of carotenoid biosynthetic gene from Xanthophyllomyces

6 dendrorhous[j ]. Mol Gen Genet, 1999, 262 : [27 ] HOSHINO T, OJ IMA K, SETOGUCHI Y. Astaxanthin synthetase : EP, A[ P] [28 ] ADRIO J L, VEIGA M. Transformation of the astaxanthin2 producting yeast Phaffia rhodozyma [ J ]. Biotechnol Techniques, 1999, 9 : [29 ] WERYJ, GUTKER D, RENNIERS A C H M. High copy number integration into the ribosomal DNA of the yeast Phaffia rhodozyma[j ]. Gene, 1997, 184 : [30 ] RUBINSTEIN L, ALTAMIRANO A, SANTOPIETRO L D, et al. Transformation of Phaffia rhodozyma by electroporation [ J ]. Biotechnology Techniques, 1996, 10 (12) : [31 ] VERDOSE J C, SANDMANN G, VISSER H, et al. Metabolic engineering of the carotenoid biosynthetic pathway in the yeast Xanthophyllomyces dendrorhous ( Phaffia rhodozyma) [J ]. Applied and Environmental Microbiology, 2003, 69 (7) : [32 ] JOHNSON E A, LEWIS M. Astaxanthin formation by the yeast Phaffia rhodozyma[j ]. J Gen Microbiol, 1979, 115 : [33 ] MEYER P S, DU PREEZ J C. Effect of culture conditions on astaxanthin production by a mutant of Phaffia rhodozyma in batch and chemostat culture [ J ]. Appl Microbiol Biotechnol, 1994, 40 : [34 ] YAMANE Y I, HIGASHIDA K, NAKASHIMADA, et al. Influence of oxygen and glucose on primary metabolism and astaxanthin production by Phaffia rhodozyma in batch and fed2batch cultures : Kinetic and stoichiometric analysis[j ]. Appl Environ Microbiol, 1997, 63 : [35 ] YAMANE Y I, HIGASHIDA K, NAKASHIMADA, et al. Astaxanthin production by Phaffia rhodozyma enhanced in fed2batch culture with glucose and ethanol feeding [ J ]. Biotechnology Letters, 1997, 19 (11) : [36 ] RAMIREZ J, GUTIERREZ H, GSCHAEDLER A. Optimization of astaxanthin production by Phaffia rhodozyma through factorial design and response surface methodology [J ]. Journal of Biotechnology, 2001, 88 : [37 ] CRUZJ M, PARAJO J C. Improved astaxanthin production by Xanthophyllomyces dendrorhous growing on enzymatic wood hydrolysates containing glucose and cellobiose [ J ]. Food Chemistry, 1998, 63 (4) : [38 ] HAARD N F. Astaxanthin formation by the yeast Phaffia rhodozyma on malasses [ J ]. Biotechnol Lett, 1998, 10 : [39 ] FLORES2COTERA L B, MARTIN R, SANCHEZ S. Citrate a possible precursor of astaxanthin in Phaffia rhodozyma : Influence of varying levels of ammonium, phosphate and citrate in a chemically defined medium[j ]. Appl Microbiol Biotechnol, 2001, 55 :

Σχετικά έγγραφα

,35 , 96 %, China Academic Journal Electronic Publishing House. All rights reserved. CHINA BIOTECHNOLOGY ( )

,35 , 96 %, China Academic Journal Electronic Publishing House. All rights reserved. CHINA BIOTECHNOLOGY ( ) 23 9 CHINA BIOTECHNOLOGY 2003 9 3 ( 310014),,, : C 40 H 52 O 2, 56419 [1 ], 1950,,, [2 ], [3 5 ] 1984 FDAΠWHO,,,, 96 %, [1 ] 1,,, 210, :2003204203 :2003207208 3 :stxp108 @yahoo. com. cn (,, 2 24,4 2 ),,,,