ADVANCES IN RESEARCH OF ANTIOXIDANT ACTIVITIES OF FLOWERS

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1 ( ) Feb JOURNAL OF SOUTH CHINA NORMAL UNIVERSITY (NATURAL SCIENCE EDITION) No. 1, 2004 : (2004) ,,, (,, ) :. : ; ; :Q946 :A ADVANCES IN RESEARCH OF ANTIOXIDANT ACTIVITIES OF FLOWERS PENG Yong - hong, ZENG You - wei, XU Liang - xiong, LONG Gang (College of Life Science, Guangdong Key Lab of Biotechnology for Plant Development, South China Normal University, Guangzhou , China) Abstract : The current situation and the newest progress are reviewed in antioxidant activities of flowers, compounds isolated from flowers, determination methods of the antioxidant activity and health - keeping function. Key words : flowers ; antioxidant ; evaluation method 1955 Haman [1 ],. [2 ]., (LPO), DNA,,, [3 ].,,. [4 ],,., V c V E (BHA) (BHT) (PG),.,.,.,, : : (003062) : ( ),,,,,.

2 1 : 137 [5 9 ]. 16, [10 ],,,, , 303, 74,178, 70 [11 ]. 22,,,V A V B V C V E V pp,, [12 ]. 112, ; ; [13 ].,,,,., V c 50. V c,v c [14 ]. V E, DNA [15 ]. [16,17 ] DPPH(1,1 - ), (TBHQ),, TBHQ. Fe 2 + H 2 O 2 [18 ].,011 % 0102 % [19 ]. ( Capparis spinosa L. ) Fe 2 + DPPH,1 000 g/ ml (rutin), BHT [20 ]. ( Hibiscus sabdarif2 f a L. ) [21 ]., [22,23 ], ( Dendranthema morifolium) [24 ], (cyanidin) V c 20,V E 50, V c., [25 27 ].,, [28 ]. - ( Spartium junceum L. ) 4 - [29 ], [30 ]. Cichewicz [31 ] ( Hemerocallis spp. ) (stelladerol), LUV TBHQ BHA BHT V E. [32 ].

3 138 ( ) 2004,,. 2 (O 2 ) (. OH) ( 1 O 2 ) (ONOO - ),. 211 (SOD) (CAT) (POD) ( GSH - Px). SOD, [33 ], : 2O2 + 2H + SOD H 2 O 2 (1) SOD O 2, CAT POD, O ( GSH) ( V A V E V C V K ) - ( ) ( ). 3, ( - SH). H 2 O 2, ( GSH - Px), H 2 O 2 H 2 O [34 ]. H 2 O 2 + 2GSH GSH - P X 2H2 O + GSSG (2) ROOH + 2GSH GSH - P X ROH( ) + GSSG+ H2 O (3),,. 2GSH + 2R. GSSR + 2RH (4) V c V E, V [35 c V ] E., (thioredoxin reductase, TR),, [36 38 ] ; (Cu,Zn - SOD,Mn - SOD), [39 ]. O 2 OH [40 ]., ( ) [41 ].,, [42 ].,B,C3 - OH,,.,

4 1 : 139 MDA, [43 ] [19 ] [19 ] (thiocyanate) [44 ] (TBA) [45 ] Fe 2 + PUFA [46 ]., (PUFA),. 312 ORAC (automated oxygen radical absorbance capacity assay) [47 ] TRAP (total radical trapping antioxidant potential) [48 ] DMPD (N,N - diphenyl - p - phenylendiamine) [49 ] DPPH (1,1 - diphenyl picrylhydrazyl) [50 52 ] TEAC - (trolox equivalent antioxidant capacity) [53,54 ] FRAP (ferric reducing ability of plasma) [48 ] - Fe 2 + [55 ].,,. 313 (SOD) (CAT) ( GSH - Px) ( GR) [48 ]. O 2 O 2, SOD ; H 2 O 2 H 2 O O 2 CAT ; ( GSH), GSH GSH - Px ; ( GSSG) (NADPH) GSSG GSH GR. 314 BR (Briggs - Rauscher reaction method).,, [56 ]. BR BR, [57 ].,.,,, [58 ] ; TBA (TBAS), [46 ]. ORAC ; FRAP, ; DMPD TRAP ph ; ;BR., DPPH, [59 ] ,,

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