Chinese Journal of Biochemistry and Molecular Biology ( HDL2C) (NLDL2C) apoa apob100, , 14 BMI < 19

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1 ISSN CN ΠQ Chinese Journal of Biochemistry and Molecular Biology 23 (9) : apoa apob100 apoa ΠapoB100 2),,,,,, 2) 3,, (, ; 2), (BMI) apoa,apob100 apoa ΠapoB100, 27 ( ) 29 ( ), 7 d 6 d, 1 d, 8 d 14 d, ( TG) (TC) ( Glu) ( HDL2C) (NLDL2C) apoa apob100, (LDL2C) apoa ΠapoB100,,TG HDL2C ( P < 010,TC LDL2C ( P < 0100,apoA apoa ΠapoB100 ( P < 0105),apoB100, apoa ΠapoB100 ( P < 0105),apoA apob100 ; apoa apob100 apoa Π apob100 BMI, 14 BMI < 19 BMI < BMI < 29, 14 BMI < 19 apoa ( P < 0105) apoa ΠapoB100 ( P < BMI < BMI < 29 apoa ( P < 0105),19 BMI < 23 apob100 ( P < 0105), apoa ΠapoB BMI < BMI < 23 ( P < BMI < 19 apoa ΠapoB100 ( P < 0105), BMI apoa,apob100 apoa ΠapoB100 ; ; ; R3 ;Q54 Changes of Apolipoprotein A, B100 and apoa ΠapoB100 : , : (No NCET ) (No ) 3 Tel : ; E2mail com Received : March 21,2007, Accepted : June 1, 2007 Induced by HCΠLF Diet in Healthy University Students LIN Jia, GONG Ren2Rong 2), GAN Chan2Fen, TANG Hui, LI Zheng2Ke, HUANG Xin, LI Rong2Hui, DONGJian 2), FANG Ding2Zhi 3 ( Department of Biochemistry and Molecular Biology, West China Medical Center, Sichuan University, Chengdu , China ; 2) Department of Cardiac and Thoracic Surgery, West China Hospital, Sichuan University, Chengdu , China) Abstract To investigate the effects of high2carbohydrateπlow2fat ( HCΠLF) diet on the levels of serum apolipoprotein A (apoa ), apob100 and apoa ΠapoB100 ratio of healthy young subjects with different Supported by the Program for New Century Excellent Talents in University (No NCET ) and the China Medical Board (No ) 3 Corresponding author Tel : , E2mail com

2 gender and different body mass index (BMI),56 healthy university students (27 males at the age of years and 29 females at the age of years) were recruited After 7 days of regular diet, they received HCΠLF diet for 6 days In the morning of the 1 st, the 8 th, and the 14 th day, the height and the body weight of each subject were measured, and fasting venous blood samples were taken Serum triglyceride ( TG), total cholesterol (TC), glucose ( Glu), high density lipoprotein cholesterol (HDL2C), non low density lipoprotein cholesterol (NLDL2C), apoa, and apob100 were measured Low density lipoprotein cholesterol (LDL2C) and apoa ΠapoB100 ratio were calculated diet, TG ( P < 010, HDL2C ( P < 010, apoa ( P < 0105), and apoa ΠapoB100 ratio ( P < 0105) increased TC and LDL2C decreased ( P < 0100 When analyzed by gender, apoa ΠapoB100 ratio of male subjects was much higher after HCΠ LF diet than those before the diet ( P < 0105) When BMI was taken into account, apoa and apoa Π apob100 ratio were much higher after HCΠLF diet than those before the diet ( P < 0105) in the subjects with 14 BMI < 19 When analyzed by both gender and BMI, apoa increased significantly in males with 14 BMI < 19 and 23 BMI < 29 ( P < 0105), while apob100 decreased in males with 19 BMI < 23 ( P < 0105) In males with 14 BMI < 19 and 19 BMI < 23, and females with 14 BMI < 19, apoa ΠapoB100 ratio increased significantly after HCΠLF diet ( P < 0105) The results suggested that the interactions among gender, BMI, and HCΠLF diet play important roles in the changes of the levels of apoa, apob100, and apoa ΠapoB100 ratio in carbohydrate2induced hypertriglyceridemia Key words high2carbohydrateπlow2fat diet ;university students ;gender ;BMI ; apolipoprotein ( cardiovascular 1 disease,cvd), 1 1 (apolipoprotein,apo) 20 26,,,, CVD, ( ),apoa apob100, CVD,apoA ΠapoB100, 60 apoa apob100, 4, 56 27, 29 [1 3 ] 112,, ( high2carbohydrateπ low2fat diet, HCΠLF diet ) , (triglyceride,tg), apoa,apob [4,5 ] 7d 6d,,,, [6,7 ],,,,, 1 3 CVD 1 d 8 d 14 d,, ( body mass index, BMI),,HCΠLF apoa, 12 h, apob100 apoa ΠapoB100 ( triglyceride, TG) ( total cholesterol, (body mass index, TC) (glucose, Glu) BMI), ( non low density lipoprotein,nldl),, HCΠLF, ( non low density apoa apob100 apoa ΠapoB , lipoprotein cholesterol,nldl2c),

3 9 : apoa apob100 apoa ΠapoB (low density lipoprotein cholesterol,ldl2c) 2 ( high density HDL2C apoa apoa ΠapoB100 lipoprotein,hdl), HDL2C ( 8 d),bmi TC LDL2C, apoa apob100, apoa ΠapoB Table 2 The changes of BMI, serum biochemistry traits and ( gx s) T apolipoproteins of subjects in the study before and after high2 carbohydrateπlow2fat diet BMI P < HDL2CΠmg Table 1, TG ( P < 0105), HDL2C ( P < 010, GluΠmmol L ( P > 0105) Table 1 Baseline characteristics of subjects in the study AgeΠyear Males ( n = 27) Females ( n = 29) ( 14 d), TG Glu apob100 (Table 2) BMIΠkg m - 2 TGΠmg dl - 1 TCΠmg dl - 1 dl - 1 LDL2CΠmg dl - 1 apoa ΠapoB Data were collected in the morning of the 8th day (the day starting the LF2 BMIΠkg m - 2 TGΠmg dl - 1 TCΠmg dl - 1 HDL2CΠmg dl - 1 LDL2CΠmg dl - 1 GluΠmmol L HC diet ) Data were collected in the morning of the 14th day (the day after the day when the LF2HC diet was finished) Results are expressed as the means SD Significance of the difference of each variable between before and after the HCΠLF diet was analyzed by Paired2Sample T Test 3 3 P < 0105, P < 0101, 3 P < 01001, when compared with those before the HCΠLF diet Data were collected in the morning of the first day of the study 213 apoa, Results were expressed as the means SD Significance of the apob100 apoa ΠapoB100 difference of each variable between males and females were analyzed Table 3, ( 14 d) by Independent2Sample T Test apoa ΠapoB100 ( 8 d) 3 3 P < 0105, 3 P < 0101, when compared with those of males ( P < 0105) apoa apob100 apoa 2 2 ΠapoB100 Table 3 The changes of apoa, aopb100 and apoa ΠapoB100 between the males and females in the study before and after high2 carbohydrateπlow2fat diet Male ( n = 27) Female ( n = 29) P P apoa ΠapoB Data were collected in the morning of 8th day (the day starting the LF2HC diet ) Data were collected in the morning of the 14th day (the day after the day when the LF2HC diet was finished) Results are expressed as the means SD Significance of the difference of each variable between before and after the HCΠLF diet was analyzed by Paired2Sample T Test

4 BMI apoa apoa apob100 apob100 apoa ΠapoB100 BMI 3 :14 BMI < BMI < BMI < 29 BMI apoa ΠapoB100 Table 4 14 BMI < 19 apoa ( P < 0105) apoa ΠapoB100 ( P < 010, BMI Table 4 The changes of apoa, aopb100 and apoa ΠapoB100 in the subjects with different BMI before and after high2 carbohydrateπlow fat diet 14 BMI < 19 ( n = 14) 19 BMI < 23 ( n = 3 23 BMI < 29 ( n = apoa ΠapoB Data were collected in the morning of the 8th day (the day starting the LFΠHC diet ) Data were collected in the morning of the 14th day (the day after the day when the LFΠHC diet was finished) Results are expressed as the means SD Significance of the difference of each variable between before and after the HCΠLF diet was analyzed by Paired2Sample T Test 3 3 P < 0105, 3 P < 0101, when compared with those before the HCΠLF diet 215 BMI ( P < 0105),14 BMI < 19 BMI < 23 apoa,apob100 apoa ΠapoB100 Table 5 Table 6, 14 BMI < 19 apoa ΠapoB100 ( P <,14 BMI < BMI < 29 apoa ( P < 0105),19 BMI < 23 apob100,apoa ΠapoB100 ( P < 0105) ) Table 5 The changes of apoa, apob100 and apoa ΠB100 in males with different BMI before and after high2carbohydrateπlow fat diet 14 BMI < 19 ( n = 4) 19 BMI < 23 ( n = 15) 23 BMI < 29 ( n = 8) die die apoa ΠB Data were collected in the morning of the 8th day (the day starting the LFΠHC diet ) Data were collected in the morning of the 14th day (the day after the day when the LFΠHC diet was finished) Results are expressed as the means SD Significance of the difference of each variable between before and after the HCΠLF diet was analyzed by Paired2Sample T Test 3 P < 0105, when compared with those before the HCΠLF diet [8 ],Aude [9 ], 3, HDL2C Wood [10 ], TG, TC LDL2C HDL min,, TG, HDL2C ,,

5 9 : apoa apob100 apoa ΠapoB TG ( P < 010,TC ( P < 0100, HDL2C ( P < 010 apoa < 0100 ( P < 0105), LDL2C ( P Table 6 The changes of apoa, apob100 and apoa ΠB100 in females with different BMI before and after high2carbohydrateπlow fat diet apoa ΠB BMI < 19 ( n = 10) 19 BMI < 23 ( n = 16) 23 BMI < 29 ( n = 3) Data were collected in the morning of the 8th day (the day starting the LFΠHC diet ) Data were collected in the morning of the 14th day (the day after the day when the LFΠHC diet was finished) Results are expressed as the means SD Significance of the difference of each variable between before and after the HCΠLF diet was analyzed by Paired2Sample T Test 3 P < 0105, when compared with those before the HCΠLF diet [5,apoA apob100 apob100, ], LDL2C [2,3 ],, apob100 [9 apoa ], Parks [11 ], HDL2C ( P < 010, apoa ( P < 010,,, 23 BMI < 29,apoA TG ( P < 0105), apob100 [10 HDL2C ] HDL2C LDL2C apoa apob100,, HDL2C,,,, Wood [10 ], apoa 7 d, Rasouli [12 ], apoa ΠapoB100 apoa, 3 d, apoa HDL, [5 ] BMI, HDL, apob100 apoa BMI LDL, apoa BMI,BMI < 19,23 BMI < 29 HDL2C, apoa B100 ΠapoB100 apoa apob100 BMI > 23 apoa,, [12 ] apoa BMI, apoa ΠapoB100,, BMI,,14 BMI < 19

6 BMI ( P < 010, BMI lowering in insulin resistant, hypertriglyceride mice, 14 BMI < 19,,, 14 BMI < 19 apoa,,,, apoa apob100 BMI, BMI apoa apob100 apoa ΠapoB100 ( References) [ 1 ] Brousseau M E, Diffenderfer M R, Millar J S, et al Effects of cholesteryl ester transfer protein inhibition on high2density lipoprotein subspecies, apolipoprotein A2I metabolism, and fecal sterol excretion [J ] Arterioscler Thromb Vasc Biol, 2005, 25 (5) : [ 2 ] Walldius G, Jungner I Apolipoprotein B and apolipoprotein A2I : risk indicators of coronary heart disease and targets of lipid2modifying therapy[j ] J Intern Med, 2004, 255(2) : [ 3 ] Walldius G, Jungner I The apobπapoa2i ratio : a strong, new risk factor for cardiovascular disease and a target for lipid2lowering therapy 2 a review of the evidence [J ] J Intern Med, 2006, 259 (5) : [ 4 ] Rashid S, Watanabe T, Sakaue T, et al Mechanisms of HDL states : the combined effect of HDL triglyceride enrichment and elevated hepatic lipase activity[j ] Clin Biochem, 2003, 36 (6) : [ 5 ],, A,A,B100,CI,C,C, E [J ] (Liu Bing2Wen, Wu Zhao2Feng, Zhang Zu2Hui The effect of high2carbohydrate and high fat diet on serum apoa, apoa, apob100, apoci, apoc, apoc, and apoe[j ] J West China Uuiv Med Sci), 1990, 21(4) : [ 6 ] Austin M A, Hutter C M, Zimmern R L, et al Genetic causes of monogenic heterozygous familial hypercholesterolemia : A HuGE prevalence review[j ] Am J Epidemiol, 2004, 160(5) : [ 7 ] Soutar A K, Naoumova R P Mechanisms of disease : genetic causes of familial hypercholesterolemia [J ] Nat Clin Pract Cardiovasc Med, 2007, 4 (4) : [ 8 ] Griel A E, Ruder E H, Kris2Etherton P M The changing roles of dietary carbohydrates : from simple to complex [ J ] Thromb Vasc Biol, 2006, 26 (9) : Arterioscler [ 9 ] Aude W Y, Pedro M, Mehta J L Metabolic syndrome : dietary interventions [J ] Curr Opin Cardiol, 2004, 19(5) : [10 ] Hellerstein M K Carbohydrate2induced hypertriglyceridemia : modifying factors and implications for cardiovascular risk [J ] Curr Opin Lipidol, 2002, 13( :33240 [11 ] Parks E J, Hellerstein M K Carbohydrate2induced hypertriacylglycerolmia : historical perspective and review of biological mechanisms[j ] Am J Clin Nutr, 2000, 71 (2) : [12 ] Rasouli M, Kiasari A M, Mokhberi V The ratio of apobπapoa, apob and lipoprotein ( a) are the best predictors of stable coronary artery disease[j ] Clin Chem Lab Med, 2006, 44 (8) :

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