ACTA SCIENTIAE CIRCUMSTANTIAE. COD Cr,

Σχετικά έγγραφα
Gro wth Properties of Typical Water Bloom Algae in Reclaimed Water

Resurvey of Possible Seismic Fissures in the Old-Edo River in Tokyo

E#ects of Drying on Bacterial Activity and Iron Formation in Acid Sulfate Soils

ΤΜΗΜΑ ΦΥΣΙΚΩΝ ΠΟΡΩΝ & ΠΕΡΙΒΑΛΛΟΝΤΟΣ

The pathway of the ozonation of 2,4,62trichlorophenol in aqueous solution

,,, (, ) , ;,,, ; -

Identification of Fish Species using DNA Method

The toxicity of three chitin synthesis inhibitors to Calliptamus italicus Othoptera Acridoidea

Influence of Flow Rate on Nitrate Removal in Flow Process

ΠΟΛΥΤΕΧΝΕΙΟ ΚΡΗΤΗΣ ΣΧΟΛΗ ΜΗΧΑΝΙΚΩΝ ΠΕΡΙΒΑΛΛΟΝΤΟΣ ΕΡΓΑΣΤΗΡΙΟ ΔΙΑΧΕΙΡΙΣΗΣ ΤΟΞΙΚΩΝ ΚΑΙ ΕΠΙΚΙΝΔΥΝΩΝ ΑΠΟΒΛΗΤΩΝ ΔΙΠΛΩΜΑΤΙΚΗ ΕΡΓΑΣΙΑ

Nguyen Hien Trang* **

Air Drying Process of Granules and Characteristics of Their Structure

Antimicrobial Ability of Limonene, a Natural and Active Monoterpene

«Συντήρηση αχλαδιών σε νερό. υπό την παρουσία σπόρων σιναπιού (Sinapis arvensis).»

Reaction of a Platinum Electrode for the Measurement of Redox Potential of Paddy Soil

CHAPTER 25 SOLVING EQUATIONS BY ITERATIVE METHODS

Molecular evolutionary dynamics of respiratory syncytial virus group A in

Motion analysis and simulation of a stratospheric airship

ACTA SCIENTIAE CIRCUMSTANTIAE. 16S rrna PNAN5 ( Rhodococcus sp. strain PNAN5). 20. ( Institute of Microbiology, Chinese Academy of Sciences,

Optimizing Microwave-assisted Extraction Process for Paprika Red Pigments Using Response Surface Methodology

Quantitative chemical analyses of rocks with X-ray fluorescence analyzer: major and trace elements in ultrabasic rocks

ACTA SCIEN TIAE CIRCUMSTAN TIAE

Approximation Expressions for the Temperature Integral

30s 56 60s 72 60s dntp cm s s s 23

* ** *** *** Jun S HIMADA*, Kyoko O HSUMI**, Kazuhiko O HBA*** and Atsushi M ARUYAMA***

1 h, , CaCl 2. pelamis) 58.1%, (Headspace solid -phase microextraction and gas chromatography -mass spectrometry,hs -SPME - Vol. 15 No.

Accumulation of Soil Arsenic by Panax notoginseng and Its Associated Health Risk

ΠΑΝΔΠΗΣΖΜΗΟ ΠΑΣΡΩΝ ΣΜΖΜΑ ΖΛΔΚΣΡΟΛΟΓΩΝ ΜΖΥΑΝΗΚΩΝ ΚΑΗ ΣΔΥΝΟΛΟΓΗΑ ΤΠΟΛΟΓΗΣΩΝ ΣΟΜΔΑ ΤΣΖΜΑΣΩΝ ΖΛΔΚΣΡΗΚΖ ΔΝΔΡΓΔΗΑ

( HN2y Co14 Co8 Co36 Sx (2) 56 (2) ) 16S

STUDY ON SCREENING AND OPTIMIZATION OF MUNICIPAL SLUDGE DEEP DEWATERING CONDITIONERS

ΠΡΟΣΟΜΟΙΩΣΕΙΣ ΧΗΜΙΚΩΝ ΔΙΕΡΓΑΣΙΩΝ ΜΕ ΧΡΗΣΗ ΤΟΥ ASPEN HYSYS: ΕΦΑΡΜΟΓΗ ΣΤΗΝ ΕΛΛΗΝΙΚΗ ΑΕΡΟΠΟΡΙΚΗ ΒΙΟΜΗΧΑΝΙΑ

HIV HIV HIV HIV AIDS 3 :.1 /-,**1 +332


16S rrna ARDRA Q938 A (2008) : (2006BAD07B03, 2006BAD08A08, 2006BAD17B08); (NYHYZX07-050)

IL - 13 /IL - 18 ELISA PCR RT - PCR. IL - 13 IL - 18 mrna. 13 IL - 18 mrna IL - 13 /IL Th1 /Th2

Studies on the Binding Mechanism of Several Antibiotics and Human Serum Albumin

Supporting information. An unusual bifunctional Tb-MOF for highly sensing of Ba 2+ ions and remarkable selectivities of CO 2 /N 2 and CO 2 /CH 4

; +302 ; +313; +320,.

Medicago marina 2012

ΕΠΙΔΡΑΣΗ ΤΗΣ ΧΡΗΣΗΣ ΕΛΑΙΟΠΛΑΚΟΥΝΤΑ ΣΤΗΝ ΔΙΑΤΡΟΦΗ ΤΩΝ ΑΙΓΩΝ ΔΑΜΑΣΚΟΥ ΩΣ ΠΡΟΣ ΤΗΝ ΠΟΣΟΤΗΤΑ ΚΑΙ ΠΟΙΟΤΗΤΑ ΤΟΥ ΠΑΡΑΓΟΜΕΝΟΥ ΓΑΛΑΚΤΟΣ

EPS. DOI / j. issn EXTRACTION OF EXTRACELLULAR POLYMERIC SUBSTANCES FROM ACTIVATED SLUDGE IN MEMBRANE BIOREACTOR

Η ΤΟΞΙΚΟΤΗΤΑ ΤΟΥ ΒΟΡΙΟΥ(B) ΣΤΗΝ ΚΑΛΛΙΕΡΓΕΙΑ ΤΗΣ ΤΟΜΑΤΑΣ

Fenton. COD ρ NH N TP ENVIRONMENTAL PROTECTION OF CHEMICAL INDUSTRY

ΕΦΑΡΜΟΓΗ ΕΥΤΕΡΟΒΑΘΜΙΑ ΕΠΕΞΕΡΓΑΣΜΕΝΩΝ ΥΓΡΩΝ ΑΠΟΒΛΗΤΩΝ ΣΕ ΦΥΣΙΚΑ ΣΥΣΤΗΜΑΤΑ ΚΛΙΝΗΣ ΚΑΛΑΜΙΩΝ

Dietary success of a new key fish in an overfished ecosystem: evidence from fatty acid and stable isotope signatures

ΤΕΧΝΟΛΟΓΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΚΥΠΡΟΥ ΣΧΟΛΗ ΓΕΩΤΕΧΝΙΚΩΝ ΕΠΙΣΤΗΜΩΝ ΚΑΙ ΔΙΑΧΕΙΡΙΣΗΣ ΠΕΡΙΒΑΛΛΟΝΤΟΣ. Πτυχιακή εργασία

Research on the Effect and Technique of Remediation for Multi-Metal Contaminated Tailing Soils

ΤΕΧΝΟΛΟΓΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΚΥΠΡΟΥ ΣΧΟΛΗ ΓΕΩΠΟΝΙΚΩΝ ΕΠΙΣΤΗΜΩΝ ΒΙΟΤΕΧΝΟΛΟΓΙΑΣ ΚΑΙ ΕΠΙΣΤΗΜΗΣ ΤΡΟΦΙΜΩΝ. Πτυχιακή εργασία

SUPPLEMENTAL INFORMATION. Fully Automated Total Metals and Chromium Speciation Single Platform Introduction System for ICP-MS

Neutralization E#ects of Acidity of Rain by Cover Plants on Slope Land

Autoxidation of Commercial Edible Oils and Emulsified Liquid Type Dressing by the Simple Method for the Determination of Peroxide Value

[11].,, , 316 6, ,., 15.5%, 9.8%, 2006., IDF,, ,500, 2,830.,, ,200.,,, β, [12]. 90% 2,,,,, [13-15].,, [13,

ΚΛΙΜΑΤΟΛΟΓΙΑ CLIMATOLOGY

CYPRUS UNIVERSITY OF TECHNOLOGY Faculty of Geotechnical Sciences and Environmental Management Department of Environmental Science and Technology

ΔΙΠΛΩΜΑΤΙΚΗ ΕΡΓΑΣΙΑ. «Προστασία ηλεκτροδίων γείωσης από τη διάβρωση»

MOTROL. COMMISSION OF MOTORIZATION AND ENERGETICS IN AGRICULTURE 2014, Vol. 16, No. 5,

ΤΕΧΝΟΛΟΓΙΚΟ ΕΚΠΑΙΔΕΥΤΙΚΟ ΙΔΡΥΜΑ ΚΡΗΤΗΣ ΤΜΗΜΑ ΜΗΧΑΝΙΚΩΝ ΦΥΣΙΚΩΝ ΠΟΡΩΝ& ΠΕΡΙΒΑΛΛΟΝΤΟΣ

ΑΡΙΣΤΟΤΕΛΕΙΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΘΕΣΣΑΛΟΝΙΚΗΣ ΤΜΗΜΑ ΟΔΟΝΤΙΑΤΡΙΚΗΣ ΕΡΓΑΣΤΗΡΙΟ ΟΔΟΝΤΙΚΗΣ ΚΑΙ ΑΝΩΤΕΡΑΣ ΠΡΟΣΘΕΤΙΚΗΣ

ΠΟΛΥΤΕΧΝΕΙΟ ΚΡΗΤΗΣ ΣΧΟΛΗ ΜΗΧΑΝΙΚΩΝ ΠΕΡΙΒΑΛΛΟΝΤΟΣ

ΠΑΝΕΠΙΣΤΗΜΙΟ ΠΑΤΡΩΝ ΤΜΗΜΑ ΗΛΕΚΤΡΟΛΟΓΩΝ ΜΗΧΑΝΙΚΩΝ ΚΑΙ ΤΕΧΝΟΛΟΓΙΑΣ ΥΠΟΛΟΓΙΣΤΩΝ ΤΟΜΕΑΣ ΣΥΣΤΗΜΑΤΩΝ ΗΛΕΚΤΡΙΚΗΣ ΕΝΕΡΓΕΙΑΣ

ΜΟΡΙΑΚΕΣ ΜΕΘΟΔΟΙ ΚΡΙΤΗΡΙΑ ΕΠΙΛΟΓΗΣ ΑΞΙΟΛΟΓΗΣΗ

Γεωπονικό Πανεπιςτήμιο Αθηνών Τμήμα Αξιοποίηςησ Φυςικών Πόρων και Γεωργικήσ Μηχανικήσ

Development of the Nursing Program for Rehabilitation of Woman Diagnosed with Breast Cancer

Σχέση µεταξύ της Μεθόδου των ερµατοπτυχών και της Βιοηλεκτρικής Αντίστασης στον Υπολογισµό του Ποσοστού Σωµατικού Λίπους

ΠΑΝΔΠΗΣΖΜΗΟ ΠΑΣΡΩΝ ΓΗΑΣΜΖΜΑΣΗΚΟ ΠΡΟΓΡΑΜΜΑ ΜΔΣΑΠΣΤΥΗΑΚΩΝ ΠΟΤΓΩΝ «ΤΣΖΜΑΣΑ ΔΠΔΞΔΡΓΑΗΑ ΖΜΑΣΩΝ ΚΑΗ ΔΠΗΚΟΗΝΩΝΗΩΝ» ΣΜΖΜΑ ΜΖΥΑΝΗΚΩΝ Ζ/Τ ΚΑΗ ΠΛΖΡΟΦΟΡΗΚΖ

Fe II aq Fe III oxide electron transfer and Fe exchange: effect of organic carbon

ΓΕΩΠΟΝΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙΟ ΑΘΗΝΩΝ ΤΜΗΜΑ ΕΠΙΣΤΗΜΗΣ ΦΥΤΙΚΗΣ ΠΑΡΑΓΩΓΗΣ ΕΡΓΑΣΤΗΡΙΟ ΓΕΩΡΓΙΚΗΣ ΖΩΟΛΟΓΙΑΣ ΚΑΙ ΕΝΤΟΜΟΛΟΓΙΑΣ

ΓΕΩΠΟΝΙΚΟ ΠΑΝΕΠΙΣΤΗΜΙO ΑΘΗΝΩΝ ΤΜΗΜΑ ΑΞΙΟΠΟΙΗΣΗΣ ΦΥΣΙΚΩΝ ΠΟΡΩΝ & ΓΕΩΡΓΙΚΗΣ ΜΗΧΑΝΙΚΗΣ

ΔΙΔΑΚΤΟΡΙΚΗ ΔΙΑΤΡΙΒΗ ΒΙΟΛΟΓΙΚΗ ΑΝΑΓΩΓΗ ΕΞΑΣΘΕΝΟΥΣ ΧΡΩΜΙΟΥ ΜΙΧΑΗΛ Κ. ΜΙΧΑΗΛΙΔΗ

Buried Markov Model Pairwise

Mean bond enthalpy Standard enthalpy of formation Bond N H N N N N H O O O

Κάθε γνήσιο αντίγραφο φέρει υπογραφή του συγγραφέα. / Each genuine copy is signed by the author.

Μεταπτυχιακή διατριβή

Study on the Strengthen Method of Masonry Structure by Steel Truss for Collapse Prevention

Μεταπτυχιακή διατριβή. Ανδρέας Παπαευσταθίου

Φπκαηίσζε: ε επηδεκηνινγηθή δηεξεύλεζε θξνπζκάησλ κε κνξηαθέο ηερληθέο

Supporting information. Influence of Aerosol Acidity on the Chemical Composition of Secondary Organic Aerosol from β caryophyllene

A life-table metamodel to support management of data deficient species, exemplified in sturgeons and shads. Electronic Supplementary Material

VSC STEADY2STATE MOD EL AND ITS NONL INEAR CONTROL OF VSC2HVDC SYSTEM VSC (1. , ; 2. , )

α 1 2- Cloning and Expression of alpha 1 2-fucosyltransferase in E. coli BIOTECHNOLOGY BULLETIN

Biodiesel quality and EN 14214:2012

( ) , ) , ; kg 1) 80 % kg. Vol. 28,No. 1 Jan.,2006 RESOURCES SCIENCE : (2006) ,2 ,,,, ; ;

Comparison of Evapotranspiration between Indigenous Vegetation and Invading Vegetation in a Bog

Elucidation of fine-scale genetic structure of sandfish (Holothuria scabra) populations in Papua New Guinea and northern Australia

ΜΕΤΑΠΤΥΧΙΑΚΗ ΕΡΕΥΝΗΤΙΚΗ ΔΙΑΤΡΙΒΗ

J. Dairy Sci. 93: doi: /jds American Dairy Science Association, 2010.

Assessment Method of the Pathogenic Microbial Exposure Caused by Aerosolization of Reclaimed Water

ΑΠΟΜΟΝΩΣΗ, ΤΑΥΤΟΠΟΙΗΣΗ ΜΕΘΑΝΟΤΡΟΦΩΝ ΜΙΚΡΟΟΡΓΑΝΙΣΜΩΝ ΚΑΙ ΒΙΟΛΟΓΙΚΗ ΜΕΤΑΤΡΟΠΗ ΜΕΘΑΝΙΟΥ ΣΕ ΜΕΘΑΝΟΛΗ

VBA Microsoft Excel. J. Comput. Chem. Jpn., Vol. 5, No. 1, pp (2006)

College of Life Science, Dalian Nationalities University, Dalian , PR China.

2 ~ 8 Hz Hz. Blondet 1 Trombetti 2-4 Symans 5. = - M p. M p. s 2 x p. s 2 x t x t. + C p. sx p. + K p. x p. C p. s 2. x tp x t.

Extraction, Amplification and Sequence Analysis of Xiajiadian Ancient Human Bone D NA


Audio Engineering Society. Convention Paper. Presented at the 120th Convention 2006 May Paris, France

Global energy use: Decoupling or convergence?

ΒΙΟΑΠΟΘΕΙΩΣΗ ΔΙΒΕΝΖΟΘΕΙΟΦΑΙΝΙΟΥ ΚΑΙ ΠΕΤΡΕΛΑΙΟΥ ΑΠΟ ΝΕΟ ΑΠΟΜΟΝΩΜΕΝΟ ΣΤΕΛΕΧΟΣ KLEBSIELLA SP. LAB

Αξιοποίηση Φυσικών Αντιοξειδωτικών στην Εκτροφή των Αγροτικών

HOMEWORK 4 = G. In order to plot the stress versus the stretch we define a normalized stretch:

Δυσκολίες που συναντούν οι μαθητές της Στ Δημοτικού στην κατανόηση της λειτουργίας του Συγκεντρωτικού Φακού

c Key words: cultivation of blood, two-sets blood culture, detection rate of germ Vol. 18 No

Transcript:

23 6 2003 11 ACTA SCIENTIAE CIRCUMSTANTIAE Vol. 23,No. 6 Nov.,2003 :025322468 (2003) 0620705206 :X703 :A ERIC2PCR 1,2, 3, 2 3 (11, 030006 ; 21,, 200240 ; 31, 030024) : ERIC2PCR,. 3 d 1, 8, 35153 mgπl 132182 mgπl, 0196 mgπl 34175 mgπl,cod Cr 1044110 mgπl, 7930190 mgπl. 4 COD Cr,. 2 ERIC2PCR (C s ) 9411 % 9616 %, 1177 2134 1160 2116, ERIC2PCR,. : ; ; ERIC2PCR Analysis of the microbial community of activated sludge in different aera2 tion basins within an industrial phenol remediation system by ERIC2PCR fingerprinting GAO Pingping 1,2, ZHAO Yi 3, ZHAO Liping 2 (11Institute of Biotechnology,Shanxi University,Taiyuan 030006 ; 21School of Life Science and Biotechnology, Lab of Molecular Microbial Ecology and Genomics, Shanghai Jiao Tong University,Shanghai 200240 ; 31D2 epartment of Environmental Engineering,Taiyuan University of Technology,Taiyuan 030024) Abstract :The occurrence, settleability and activity of activated sludge in first (TJ1) and second (TJ2) aeration basin in a AB process based industrial phenol remediation system were analyzed by ERIC2PCR( Enterobacterial Repetitive Intergenic Consensus2PCR) fingerprinting com2 bined with activated sludge characterization (SV 30 MLSS SVI), and major pollutants (phenol, cyanide and COD Cr ) measurements. During the monitoring period (one sampling every three days for 8 continuous samplings, M1 M8), the concentrations of primary pollutants in the influent fluctuated from 35153 mgπl to 132182 mgπl for phenol, between 0196 mgπl to 34175 mgπl for cyanide and from 1044110 mgπl to 7930190 mgπl for COD Cr 1 The presence of a pollution shock caused by mineral oil contamination occurred at M4 monitoring stage resulted in a dramatic decrease in activated sludge settleability and COD Cr removal efficiency. The introduction of this loading shock had no significant im2 pact on the system s ability to degrade phenol and cyanide. ERIC2PCR banding patterns of activated sludge samples at 8 stages showed a mean C s value of 9411 % within TJ1 and 9616 % within TJ2, Shannon2Weaver indices of 1177 2134 in TJ1 and 1160 2116 in TJ2. Populations in the system detected by ERIC2PCR fingerprinting were thus fairly stable. The stability of the ERIC2PCR amplifiable populations in activated sludge may serve as the basis for relatively stable removal efficiency of phenol and cyanide during the monitoring period. Keywords :activated sludge ; bacterial community structure ; ERIC2PCR :2003201228 ; :2003203210 : ( 863 ) (No. SZ203201204,2001AA214131) : (1957 ),, 3 E2mail :LPZhao @mail. sjtu. edu. cn

706 23 [1,2 ],,. [3 ].,,,, [4 ]., 16S rdna (DGGEΠTGGE) [1,2,4 (RFLP) 6 ]., DNA,.,. ERIC( Enterobacterial Repetitive Intergenic Consensus, ) 126bp, [7 ]. (44bp), [8 DNA, ERIC2PCR 10 ]. ERIC2PCR,, [10,11 ]., ERIC2PCR AB. 1 111. (AB), (TJ1),,, 90 % 99 % 70 % 95 %,COD Cr 70 %. 2 (TJ2) 1,. 3 d 1, 8, M1 M8. TJ1 TJ2, TJ1 TJ2. 112 (SV 30 ), (MLSS) (SVI) [12 ]. 42 ; 2 ;COD Cr [12 ]. 113 DNA DNA DNA [13 ]. DyNA Quant TM 200 (Hoefer Pharmacia Biotech) DNA.

6 : ERIC2PCR 707 114 ERIC2PCR ERIC [7 ],, : ERIC1R 5 2ATGTA2 AGCTCCTGGGGATTCAC23 ERIC2 5 2AAGTAAGTGACTGGGGTGAGCG23. [9 ]. PCR MiniCycler TM (MJ Research Inc), PCR Promega. 115 %( WΠV) ( 115 gπml) PCR, 500 ng. UVP2 GDS8000 (Ultra2Violet Product Ltd). 115 ERIC2PCR Shannon s ( H ). : H = - ( n iπn) ln ( n i ΠN), n i, N. Sorenson (pairwise similarity coeffi2 cient, C s ) ERIC2PCR [15 ]. C s = 2 j Π( a + b) 100, a ERIC2PCR, b ERIC2PCR, j. Sorenson 0, 2 DNA Sorenson 100 %. 2 211 SV 30 SVI TJ1 TJ2 ( 1). 4 (M2 M3 M5 M7) 2,SV 30 16196 % 2919 %, SVI 98137mLΠg, 202115mLΠg. M1 M6 M8 3,SV 30 30158 %, 5519 %,M8 SVI 298122mLΠg. M4,,2,SV 30 100 %,SVI 351146mLΠg 365183 mlπg. 2 1 TJ1 TJ2 SV 30 SVI,TJ2 TJ1. 212 COD Cr TJ1 COD Cr, 104411 793019 mgπl. Fig. 1 Fluctuation of SV 30 and SVI of activated sludge in first (TJ1) and second(tj2) aeration basin during monitoring period,. M1 34175 mgπl, 3122 ; M3 M8 0196 7125 mgπl. M2, 3515 mgπl ; M8, 132182 mgπl, 3174 ; 6 4316 8019 mgπl. TJ1, TJ2 ( 2). 213 TJ1, 94189 % 99144 % ; 6612 % 9813 % ; COD Cr, 7413 %, 30 % 45 %,M4 COD Cr. TJ2 COD Cr

708 23 9512 %,8912 % 2618 % ; M4 TJ2 COD Cr, M7, M8 COD Cr 19178 %( 3). 2 TJ1 TJ2 COD Cr Fig. 2 Fluctuation of phenol,cn - and COD Cr in influent water to TJ1 and TJ2 during monitoring period 3 TJ1 TJ2 COD Cr Fig. 3 Fluctuation of removal efficiency of phenol, cyanide and COD Cr by activated sludge in TJ1 and TJ2 during monitoring period 214 2 8 DNA ERIC2PCR. TJ1 TJ2, ( 4A 4B). 8 2 ERIC2PCR,. TJ1 ERIC2 PCR C s 87 %, 100 %, C s 9411 %( 1). TJ1 M3 H (1177), 7 4 8 TJ1 TJ2 ERC2PCR Fig. 4 ERIC2PCR fingerprints of activated sludge from TJ1 and TJ2 during experiment (Lanes :1. 1 kb 1adder ;2. negative control ; 3 10. activated samples obtained from first to eight dates, A :TJ1 ;B :TJ2) 5 8 TJ1 TJ2 Fig. 5 Shannon index of diversity of the activated sludge microbial community from TJ1 and TJ2 during experiment (M1 M8,activated samples obtained from first to eight dates,a :TJ1 ;B :TJ2)

6 : ERIC2PCR 709 H 2105 2134 ( 5A). TJ2 ERIC2PCR,8 ERIC2PCR 9616 %( 1), 1160 2116 ( 5B). 1 8 TJ1 TJ2 ERIC2PCR Table 1 C s matrix values for individual activated sludge samples from TJ1 and TJ2 TJ1 C s, % M1 M2 M3 M4 M5 M6 M7 M1 M2 M3 M4 M5 M6 M7 M2 96 88 M3 92 96 88 100 M4 96 92 96 88 100 100 M5 92 88 92 96 92 96 96 96 M6 92 88 92 96 100 92 96 96 96 100 M7 92 88 92 96 100 100 92 96 96 96 100 100 M8 96 92 96 100 96 96 87 92 96 96 96 100 100 100 TJ2 3 ERIC2PCR AB 2 8.,,. M4,,2. TJ1 COD Cr, TJ2 COD Cr 2.,. Whiteley [3 ] DGGE FISH rrna,, (microbial densities). Watanabe TGGE,,, 115 gπ(l d),, 2 [6 ]., COD Cr, COD Cr, ERIC2PCR, TJ1,,.,ERIC2PCR ERIC DNA [14 ]., ERIC2PCR,,,.,

710 23,.,ERIC2PCR,. : [ 1 ] Eichner C A,Erb R W, Timmis K N, et al. Thermal gradient gel electrophoresis analysis of bioprotection from pollutant shocks in the activated sludge microbial community[j ]. Appl Environ Microbiol, 1999, 65 (1) : 102 109 [ 2 ] Marsh T L,Liu Wen2Tso,Forney L J, et al. Beginning a molecular analysis of the eukaryal community in activated sludge[j ]. Wat Sci Tech, 1998, 37 (4 5) : 455 460 [ 3 ] Whiteley A S, Bailey M J. Bacterial community structure and physiological state within an industrial phenol bioremediation system [J ]. Appl Environ Microbiol, 2000, 66 (6) : 2400 2407 [ 4 ] Wagner M, Amann R, Lemmer H. Probing activated sludge with oligonucleotides specific for proteobacteria : inadequacy of culture2 dependent methods for describing microbial community structrue[j ]. Appl Environ Microbiol, 1993, 59 (5) : 1520 1525 [ 5 ] Vainio E J, Moilanen A, Koivula T T, et al. Comparison of partial 16S rrna gene sequences obtained from activated sludge bacte2 ria. Appl Microbiol Biotechnol, 1997, 48 (1) : 73 79 [ 6 ] Watanabe K,Teramoto M, Harayama S. An outbreak of nonflocculating catabolic populations caused the breakdown of a phenol2di2 gesting activated2sludge process[j ]. Appl Environ Microbiol, 1999, 65 (7) : 2813 2819 [ 7 ] Versalovic J, Koeuth T, Lupski J R. Distribution of repetitive DNA sequence in eubacteria and application to fingerprinting of bacte2 rial genomes[j ]. Nucleic Acids Res, 1991, 19 : 6823 6831 [ 8 ] De Bruijn F J. Use of repetitive (repetitive extragenic palindromic and enterobacteria repetitive intergeneric consensus) sequence and the polymerase chain reaction to fingerprint the genomes of Rhizobium meliloti isolates and other soil bacteria[j ]. Appl Environ Mi2 crobiol, 1992, 58 (2) : 2180 2187 [ 9 ] Di Giovanni G D,Watrud L S, Seidler R J. Fingerprinting of mixed bacteria strains and BIOLOG Gram2Negative ( GN) substrate communities by enterobacterial repetitive intergenic consensus sequence2pcr ( ERIC2PCR) [J ]. Current Microbiology, 1999, 38 : 217 223 [10 ] L Zhao, P Gao, et al. ERIC2PCR as a community fingerprinting technology for identification of functional components in activated sludge for wastewater treatment in coke industry[ Z]. Proceedings of 101st Annual Meeting of American Society of Microbiology, 2001, Orlando, FL. p600 [ 11 ] G Song, G Wei, Y Cao, et al. Use of ERIC2PCR as a community fingerprinting technology in elucidation of mode of action of a new probiotic formulation for prevention and treatment of bacterial diarrhea in piglets[a]. Book of abstracts of the Asia Pacific Conference on Plant Tissue Culture & Agribiotechnology 19 23, Novemeber, 2000, Singapore G16. 166 [12 ], (, ). [M]. :, 1985. 469 472 [13 ],. DNA [J ].,2002, 22 (11) : 2015 2020 [14 ],,. MG1655 ERIC2PCR [J ].,2002, 29 (6) : 28 32 [15 ] Murray A E,Hollibaugh J T,Orrego C. Phylogenetic composition of bacterioplankton from two Califorhia estuaries compared by dena2 turing gradient gel electrophoresis of 16S rdna fragments. Appl Enriron Microbiol,1996,62 (7) :2676 2680