Fifty years of amphioxus study in China

20 1 2008 2 Chinese Bulletin of Life Sciences Vol. 20, No. 1 Feb., 2008 1004-0374(2008)01-0064-05 50, 266003 50, Q959.287A Fifty years of amphioxus study in China ZHANG Shi-cui*, GUO Bin, LIANG Yu-jun (Department of Marine Biology, Ocean University of China, Qingdao 266003, China) Abstract: Amphioxus is becoming an emerging model organism for insights into the origin and evolution of vertebrates. A great progress has been made in the study of amphioxus biology in China in the past 5 decades, and this has been dealt with in the present review. Key words: amphioxus; development; evolution; immunity 2001 The development of isolated blastomeres of amphioxus [1] 50 1 Wilson 2-4- 1/2 1/4 1/4 4 40 Conklin [2] Wilson 2-1/2 4-2/4 2/4 4-1/4 2- Wilson Conklin Tung 2-32- Wilson Conklin [1,3-5] 8-32- ( 1) 8-2/3 2007-07-25 2007-09-03 (2004BS06017) E-mail: sczhang@ouc.edu.cn

50 65 1 8-32- endo: ep: m: n: ch: 1/3 32- an 1 an 2 an 2 veg 1 veg 1 veg 2 veg 2 veg 1 veg 2 veg 2 veg 2 veg 1 veg 1 an 1 veg 2 an 1 +veg 2 [3,4] Conklin [2] [6] 32-64- [7] 32-64- Zhang [8] Ca 20 90 Hox Zhang [9] Brachyury Li [10] ApoD hedgehog TCTP p23 som mdp GABARAPL2 [11-16] 2 (Hatschek s pit) - - - -

66 [17] Chang [18] (LH) (FSH) [19] (GnRH-A) [20] - β- (hlhβ) (hcg) [21] (GTH) [22] [23] Sephadex G-25 (LHRH) LHRH LHRH LHRH LHRH [24] LHRH [25] - - 3 ( 2) 2 (vitellogenin, Vg) (alanine aminotransferase, AAT) (antithrombin, AT) (plasminogen, Pg) Vg Vg Vg [26] AAT AT Pg Pg AAT AT Pg [27-29] S- (glutathione S-transferase, GST) [30] GST GST GST GST 4 Metchnikoff 1892

50 67 Zhang [31] 20 80 21 Wang [32] [32-34] [35] Mg 2+ C3 C3 C3 B (factor B, Bf) (annose-binding lectin-associated serine proteases) (acute phase response) (acute phase proteins, APPs) AT AAT [27,29] AT AAT AT AAT AT AAT V (Ig V region-containing chitin-binding proteins, VCBPs) [36] VCBPs 5 MHC (anchor genes) MHC MHC MHC [37] V(D)J RAG1 [38,39] V C (V and C domain-bearing protein, VCP) Huang [40] 50 10 [1] Tung TC, Wu SC, Tung YYF. The development of isolated blastomeres of amphioxus. Sci Sin, 1958, 7(12): 1280-319 [2] Conklin EG. The development of isolated and partially separated blastomeres of amphioxus. J Exp Zool,1932, 64(2): 303-74 [3] Tung TC, Wu SC, Tung YYF. The developmental potencies of the blastomere layers in amphioxus egges at the 32-cell stage. Sci. Sin, 1959, 9: 119-41 [4] Tung TC, Wu SC, Tung YYF. Rotation of the animal blastomeres in amphioxus eggs at the 8-cell stage. Sci Record New Ser. 1960, 4: 389-94 [5] Tung TC, Wu SC, Tung YYF. The presumptive area of the egg of amphioxus. Sci Sin, 1963, 11: 82-90 [6] Tung TC, Wu SC, Tung YYF. Experimental studies on the neural induction in amphioxus. Acta Biol Exp Sin, 1961, 7: 263-70 [7] Tung TC, Wu SC, Tung YYF. Differentiation of the pro-

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