Endogenous Development of Eimeria flavescens in Domestic Rabbit

Chinese Journal of Zoology 2010 45 3 91 ~ 97 1 1 1 1* 1 2 1 450002 2 100094 Oryctolagus curiculus Eimeria flavescens 20 4 1 2 1 60 ~ 72 h 2 84 h 3 96 ~ 120 h 4 144 ~ 153 h 167 h 215 h 2 Q38 A 0250-3263 2010 03-91-07 Endogenous Development of Eimeria flavescens in Domestic Rabbit WANG Yan 1 ZHAO Jin-Feng 1 WANG Qiang 1 ZHANG Long-Xian 1 * NING Chang-Shen 1 LIN Kun-Hua 2 1 College of Animal Science and Veterinary MedicineHenan Agricultural UniversityZhengzhou 450002 2 College of Veterinary MedicineChina Agricultural UniversityBeijing 100094China Abstract In order to understand the endogenous development of Eimeria flavescens20 coccidia-free rabbits Oryctolagus curiculus were orally inoculated with E. flavescens sporulated oocysts and killed under anesthesia at intervals for collecting intestinal tissue samples. The samples were used for conventional paraffin section and H. E staining. Four asexual generations and one sexual generation. Two types of meronts and merozoites were found in each asexual generation type A meronts gave rise to a smaller number of thick and short merozoites while the type B meronts produced lots of thin and long merozoites. The first generation meronts were found in the crypts of the jejunum 60-72 h after inoculation a. i.. The second generation meronts were found in the crypts of the large intestine 84 h after inoculation. The third generation meronts developed in the villi of the large intestine 96-120 h after inoculation. The fourth generation meronts were observed in the crypts of the large intestine 144-153 h after inoculation. The gamogony took place in large intestine 167 h after inoculation. The mature oocysts were found 215 h after inoculation. Key words Domestic Rabbit Oryctolagus curiculus Endogenous development Histology Eimeria flavescens Eimeria flavescens Eimeria pellerdyi 2 Norton Oryctolagus curiculus 1 E. irresidua Coudert 1977 No. 81100912300 No. 2005DKA 21104 * E-mail zhanglx8999@ yahoo. com. cn E-mail Wangyanjmyg@ yahoo. com. cn 2009-09-16 2009-12-20

92 Chinese Journal of Zoology 45 Marotel Guilhon 3 1 4-5 1. 2 6 1 / d 1 40 3 1 1. 3 22 1. 1 12 1 Table 1 Sampling times and infective dosages of experimental rabbits No. Dose of oocysts per rabbit h Sampling time Hours after infection No. Dose of oocysts per rabbit h Sampling time Hours after infection 1 2 10 7 12 11 1. 47 10 6 144 2 2 10 7 24 12 5 10 5 153 3 2 10 7 36 13 2 10 5 167 4 2 10 7 48 14 2 10 5 177 5 2 10 7 60 15 2 10 5 190 6 2 10 7 72 16 2 10 5 201 7 2 10 7 84 17 2 10 5 215 8 2 10 7 96 18 2 10 5 225 9 8. 8 10 5 108 19 2 10 5 239 10 2 10 7 120 20 2 10 5 249 1. 4 1 Ⅰ 2 3 A B 2 1 A 8 ~ 18 H. E 2 215 h 1. 9 ~ 2. 4 μm 4. 7 μm 2. 1 μm 2 84 h 2 2 2. 1 24 h Ⅰ 1 10. 8 ~ 11. 6 μm 1. 1 ~ 1. 9 μm 11. 1 μm 1. 5 μm B 2 ~ 4 4. 2 ~ 5. 3 μm Ⅰ 4 5 2 A 12 ~ 36 9. 9 ~ 10. 7 μm 1. 1 ~ 2. 2 μm 10. 3 μm 1. 7 μm B 60 ~ 72 h 1 2 ~ 4 5. 0 ~ 5. 7 μm 1. 5 ~ 2. 1 μm 5. 3 μm 1. 8 μm 2

3 93 Table 2 2 The endogenous developmental stages of Eimeria flavescens Developmental stage Spot μm Mean merozoite size h Hours after infection Sporozoite Small intestinal lamina propria 24 ~ 48 1 The first generation meront Crypts epithelium of the small intestine A 11. 1 1. 5 B 4. 7 2. 1 60 ~ 72 2 The second generation meront Crypt epithelium of the large intestine caecum and colon A 10. 3 1. 7 B 5. 3 1. 8 84 3 The second generation meront Mucous membrane epithelium of large intestine caecum and colon A 10. 0 1. 4 B 5. 1 1. 6 96 ~ 120 4 The fourth generation meront Crypt epithelium of the large intestine caecum and colon A 7. 5 1. 2 B 4. 2 1. 4 144 ~ 153 Gamogony Large intestine caecum and colon 167 Oocysts Large intestine caecum and colon 215 3 96 ~ 120 h Ⅰ 6 7 3 A 9 ~ 18 9. 6 ~ 10. 4 μm 1. 1 ~ 1. 8 μm 10 μm 1. 4 μm B 2 ~ 4 4. 7 ~ 5. 4 μm 1. 3 ~ 1. 9 μm 5. 1 μm 1. 6 μm 2 144 ~ 153 h 4 Ⅱ 11 23. 0 ~ 23. 9 μm 23. 7 ~ 24. 4 μm 23. 4 μm Ⅰ 8 Ⅱ 9 10 4 A 16 ~ 24 7. 1 ~ 7. 9 μm 0. 9 ~ 1. 4 μm 7. 5 μm 1. 2 μm B 2 ~ 4 4. 0 ~ 4. 4 μm 1. 1 ~ 1. 6 μm 4. 2 μm 1. 4 μm 2 24 μm 2. 2 167 h Ⅱ 12 4 4 2. 3 201 ~ 215 h

94 Chinese Journal of Zoology 45 Ⅱ 13 14 144 h 3 4 16 3. 1 3 Pellerdy 17 5 6 Soulsby 18 Rebert 19 E. intestinalis E. larimerensis 3 s 4 Pakandl 20 7 1 min E. coecicola 8 4 Pakandl 21 E. vejdovskyi 9-12 Jensen 13 5 Shazly 22 E. magna 5 2 10 7 14 2 Norton 1 E. stiedai 108 h 3. 2 Norton 1 Pakandl 15 A 5 2 B Pellerdy 17 3 2 3 108 h 4 3 Pakandl 19 15 5 2 Pakandl 21 108 h 120 ~ A B 2 Shazly 177 h Norton 1 4 22 A B 2 108 ~ 120 h 84 h 96 ~ 120 h 1 Norton C CCatchpole JJoyner L P. Redescriptions of

3 95 Eimeria irresidua Kessel & Jankiewicz1931 and E. flavescens Marotel & Guilhon1941 from the domestic rabbit. Parasitology197979 231-248. 2 Coudert P. Isolation and description of a new species of Eimeria in rabbits Orychtolagus cuniculus Eimeria pellerdyi P. Coudert1977 SporozoaCoccidia. C R Acad Sci Paris Sèr D 1977285 8 885-887. 3 Marotel GGuilhon J. Recherches sur la coccidiose du lapin. Rec Med Vet 1941 117 321-328. 4. Eimeria flavescens Eimeria Marotel and Guilhon1941 an electron. 1995 26 4 341-351. microscopic study of its life cycle. Parasitol Res 200391 5. Eimeria 4 304-311. flavescens. 16.. 1994 20 3 322-326. 1989 12 2 97-101. 6. Eimeria 17Pellerdy L. Coccidia and Coccidiosis 2nd ed. Berlin flavescens. and Hamburg Paul Parey 1974408-445. 1994 20 3 313-320. 7 Roberts W LSpeer D AHammond D M. Penetration of Eimeria larimerensis sporozoites into cultured cells as observed with the light and electron microscope. J Parasitol197157 615-625. 8 Seville R SStanton N L. Synonymy of Eimeria larimerensis with Eimeria lateralis. J Parasitol1993 79 6 970-972. 9 Strout R GSalis JSmith S Cet al. In vitro cultivation of Eimeria acervulina Coccidia. Exp Parasitol1965 17 241-246. 10Jensen S BHammond D M. Penetration of Eimeria magna into cultured cells. J Prasitol197449 428-432. 11Drouet-Viard ELicois DProvot Fet al. The invasion of the rabbit intestinal tract by Eimeria intestinalis sporozoites. Parasitol Res 199480 706-707. 12Renaux SDrouet-Viard FChanteloup N Ket al. Tissues and cells involved in the invasion of the rabbit intestinal tract by sporozoites of Eimeria coecicola. Parasitol Res 200187 98-106. 13Jensen S BEdgar S A. Effects of antiphagocytic agents on penetration of Eimeria magna into cultured cells. J Parasitol197656 656-662. 14.. 1994 25 3 252-255. 15Pakandl MCerník FCoudert P. The rabbit coccidium 18Soulsby E J L. HelminthsArthropods and Protozoa of Domesticated Animals 7th ed. LondonUK Bailliere Tindall 1982657-661. 19.. 1993 39 2 189-198. 20Pakandl MCoudert PLicois D. Migration of sporozoites and merogony of Eimeria coecicola in gut-associated lymphoid tissue. Parasitol Res 199379 593-598. 21Pakandl MCoudert P. Life cycle of Eimeria vejdovskyi Pakandl1988 electron microscopy study. Parasitol Res 199985 850-854. 22Shazly MMuborak MAl-Rasheid K A Set al. Light and electron microscopic studies of Eimeria magna infecting the domestic rabbit Oryctolagus cuniculus from Saudi Arabia. I. Asexual developmental cycles. Saudi Journal of Biol Sci 200512 1 1-10. Ⅰ 1. 2. 1 3. 1 4. 2 5. 2 6. 3 7. 3 8. 4 1 000 Explanation of Plate Ⅰ 1. The sporozoites in the leucocyte 2. The first generation merontmerozoites tape A in the epithelium of the jejunum 3. The first generation merontmerozoites tape B in the glands 4. The second generation merontmerozoites tape A in the glands of the caecum 5. The second generation merontmerozoites tape B in the glands of the caecum 6. The third generation merontmerozoites tape B in the glands of the caecum 7. The second generation merontmerozoites tape A in the epithelium of the colon 8. The second generation merontmerozoites tape A in the glands of the caecum. 1 000

96 Chinese Journal of Zoology 45 WANG Yan et al. Endogenous Development of Eimeria flavescens in Domestic Rabbit Ⅰ Plate Ⅰ

3 97 WANG Yan et al. Endogenous Development of Eimeria flavescens in Domestic Rabbit Ⅱ Plate Ⅱ 9. 4 1 000 10. 4 1 000 11. 1 000 12. 1 000 13. 1 000 14. 400 9. The fourth generation meront tape B in the glands of the colon 1 000 10. The fourth generation meront tape A in the procussus vermiformis 1 000 11. The macrogametocyte in the glands of the caecum 1 000 12. The mature microgametocyte in the glands of the caecum 1 000 13. The zygote in the glands of the colon 1 000 14. The oocysts in the glands of the colon 400.