2010 37 6971 976 Acta Horticulturae Sinica 5 1,2 1,* 1 1 150040 2 150025 GC MS 23 30 11 64.73% α 30.67% 14.92% 13 61.41%α 28.98% 14.70%α 3 50 µl ml -1 5 100% µl ml -1 92% 85% 23% 5 µl ml -1 60% 25% 74% α> > S 687.3 A 0513-353X 2010 06-0971-06 The Volatile Constituents Analysis of Scindapsus aureum and Hedera nepalensis var. sinensis and Their Inhibition Against Five Fungi MENG Xue 1,2 WANG Zhi-ying 1,* and Lü Hui 1 1 Forestry Institute Northeast Forestry University Harbin 150040 China 2 Heilongjiang Ecological Engineering Training College Harbin 150025 China Abstract The volatile constituents of bunting Scindapsus aureum and Chinese ivy Hedera nepalensis var. sinensis were analysed by dynamic headspace-gc-ms analysis. Twenty-three volatile compounds were preliminary determined including 11 terpene compounds accounting for 64.73% α-pinene and camphene were accounted for 30.67% and 14.92% respectively in bunting. Thirty volatile compounds were preliminary determined including 13 terpene compounds accounting for 61.41% α-pinene and camphene were accounted for 28.98% and 14.70% respectively in Chinese ivy. The inhibitory effect of α-pinene camphene and eucalyptole against Penicillium sp. Aspergillus niger Cladosporium sp. Epicoccum sp. and Alternaria sp. were detected. The results showed that the inhibition rates were all 100% at 3 terpene monomers concentration of 50 µl ml -1. The inhibition rates were 92% 85% and 23% at the concentration of 10 µl ml -1 respectively against Aspergillus niger and were 60% 25% and 74% at the concentration of 5 µl ml -1 respectively against Penicillium sp. The bacteriostatic 2009 11 23 2010 05 06 GB08B202 * Author for correspondence E-mail zyw0451@sohu.com
972 7 3 effect α -pinene was the best eucalyptole was second and camphene was the worst in generally. Key words Hedera nepalensis K. Koch var. sinensis Tobl. Rehd. Epipremnum pinnatum L. Engl. organic volatile matter fungi inhibition 50 8 2007 9 38.24% 28.38% 2009 6 6 ATCC29213 DH10B FM-S2 2007 8 8 α 1 1.1 Hedera nepalensis K. Koch var.sinensis Tobl. Rehd. Epipremnum pinnatum L. Engl. Penicillium sp. Aspergillus niger Cladosporium sp. Epicoccumsp. Alternaria sp. α α-pinene Eucalyptole Camphene SIGMA-ALDRICH 1.2 GC/MS 2008 6 25 ~ 30 70% ~ 80% 12:00 13:00 15 min 2002 ENTECH7100 GC-17G GC-MS-QP5050 400 ml 2007 GC Cp2Sil CB 30 m 0.25 mm 0.25 µm 6 kpa 250 230 10 1 0 2 min 5 min -1 100 0.5 min 20 min -1 250 250 8 min
6 5 973 MS El 70 ev 200 mpz 10 ~ 400 0.14 sp NIST 2.0 EVA 1994 2003 2003 1.3 α 10 50 100 200 500 µl ml -1 1 ml 9 ml 55 PDA 1 5 10 20 50 µl ml -1 0.5 cm 3 PDA PDA 25 3 d % = / 100 2 2.1 GC MS 1 1 2 0.1% 28 23 11 6 1 2 64.73% 3.43% 0.4% 0.45% 45 30 13 6 1 1 3 3 61.41% 2.93% 0.21% 0.39% 1.61% 0.51% 1 Fig.1 GC/MS total ion current chromatogram of the volatile matter from Epipremnum pinnatum L. Engl. and Hedera nepalensis K. Koch var. sinensis (Tobl.) Rehd.
974 7 3 Table 1 1 The compositional analysis of volatile matter from Epipremnum pinnatum L. Engl. No. /% Relative content Similarity Molecular formula Compound 1 7.96 92 C 4 H 8 (Z) 2 Cis-2-butene 2.267 2 1.70 92 C 8 H 18 2 2 4 2 4-trimethylpentane 2 2.367 3 2.09 84 C 7 H 15 NO 4 (2) 4-(2-aminoethyl)tetrahydropan 2.475 4 0.40 91 C 6 H 12 O 13 1-hexen-3-ol 2.642 5 0.21 83 C 13 H 16 O 4 Diethyl phenylmalonate 2.967 6 0.10 67 C 6 H 6 Benzene 3.275 7 0.13 87 C 10 H 20 Tert-butylcyclohexane 5.467 9 0.81 98 C 10 H 16 Tricyclene 8.717 10 30.67 98 C 10 H 16 α α-pinene 9.092 11 14.92 98 C 10 H 16 Camphene 9.608 12 0.56 93 C 10 H 16 Sabinene 10.350 13 6.18 98 C 10 H 16 β β-pinene 10.500 15 1.63 93 C 10 H 16 Laurene 10.900 16 0.26 92 C 10 H 14 O-cymene 12.050 18 0.19 93 C 11 H 24 N-undecane 14.492 20 0.24 97 C 12 H 26 Dodecane 16.550 21 0.24 92 C 11 H 18 O 2 Isobornyl formate 17.842 22 0.40 95 C 12 H 26 Laurane 17.950 23 0.54 91 C 15 H 24 α α-longipinene 18.608 24 0.18 85 C 15 H 24 (+) (+)-Isolongifolene 18.883 25 0.77 95 C 14 H 40 Tetradecane 18.950 26 0.59 89 C 15 H 24 Zingiberene 19.267 28 0.69 83 C 15 H 24 Cedrene 19.892 /min Retention time Table 2 2 The compositional analysis of volatile matter from Hedera nepalensis K. Koch var. sinensis Tobl. Rehd. /% No. Relative content Similarity Molecular formula Compound 1 7.21 87 C 4 H 8 (Z) 2 Cis-2-butene 2.250 2 1.41 91 C 8 H 18 2 2 4 4-trimethylpentane 2 2.359 3 2.13 89 C 5 H 10 O Methylcyclobutane 2.400 4 0.35 72 C 4 H 3 Cl 1 3 (Z)-1-chloro-1-buten-3-yne 2.517 5 1.18 89 C 8 H 14 O 2 Butyl methacrylate 2.633 6 0.18 80 C 5 H 10 O 2 Tert-butyl formate 2.692 7 0.21 87 C 6 H 12 O 2 2-ethylcyclobutanol 2.783 9 0.39 83 C 3 H 2 Cl 4 O 1 1 3 3 1 3 3-tetrachloroacetone 1 2.958 10 0.12 98 C 2 H 4 Cl 2 Dutch liquid 3.200 11 0.13 72 CCl 3 NO 2 Chlorpicrin 3.275 13 0.11 91 C 5 H 10 O 2 2-methylbutyraldehyde 3.675 14 0.12 95 C 7 H 8 1 3 5 Cycloheptatriene 4.750 17 0.81 98 C 10 H 16 Tricyclene 8.742 18 28.98 91 C 10 H 16 α α-pinene 9.167 19 14.70 97 C 10 H 16 Camphene 9.683 20 0.67 91 C 10 H 16 Sabinene 10.417 21 6.75 98 C 10 H 16 β β-pinene 10.575 24 0.20 98 C 10 H 22 Decane 11.317 25 0.23 80 C 8 H 16 O Capryl aldehyde 11.458 27 0.33 92 C 10 H 14 O-cymene 12.108 28 0.96 93 C 10 H 16 D -limonene D 12.258 29 0.50 85 C 10 H 18 O Eucalyptole 12.392 30 0.12 89 C 10 H 16 Terpinolene 14.083 31 0.24 93 C 10 H 12 2 4 4-dimethylstyrene 2 14.242 32 0.40 93 C 11 H 24 N-undecane 14.533 33 0.17 91 C 9 H 18 O 1-nonanal 14.692 36 0.15 95 C 10 H 8 Azulene 16.342 37 0.67 96 C 12 H 26 Dodecane 16.567 38 0.25 93 C 11 H 18 O 2 Isobornyl formate 17.825 41 0.60 93 C 15 H 24 β β-caryophyllene 18.608 /min Retention time
6 5 975 64.73% 61.41% 3.43% 2.93% α 30.67% 14.92% α 28.98% 14.70% 1 2 2.2 α 5 5 3 50 µl ml -1 100% α 10 µl ml -1 92% 20 µl ml -1 100% 20 µl ml -1 75% 3 α > > Terpene 3 α 5 Table 3 α-pinene eucalyptole camphene to 5 fungi inhibition rate determinations /% / µl ml -1 Concentration Cladosporium sp. Epicoccum sp. Alternaria sp. Penicillium sp. α 1 30a 8.5a 30a 29a 18a α-pinene 5 55ab 52b 62ab 60b 79b 10 73b 73bc 68bc 69bc 92c 20 81c 100cd 80c 84bc 100c 50 100c 100d 100c 100c 100c 1 24a 33a 32a 48a 49a Eucalyptole 5 48b 54b 53b 74b 72b 10 70c 67bc 71c 83bc 85b 20 81d 81c 90d 90c 100b 50 100d 100c 100d 100d 100b 1 26a 2a 28a 23a 14a Camphene 5 43b 21ab 30ab 25a 20a 10 51b 45bc 36bc 28a 23a 20 52b 75cd 40c 44b 36b 50 100c 100d 100d 100c 100c P = 0.05 Note For different fungi the same small letters mean no significant differences P = 0.05. Aspergillus niger 3 GC MS 64.73% 61.41% α α 30.67% 14.92% 28.98% 14.70% 2009 9 12 2007 GC MS GC MS
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