SGR ,,, g (16,18,20,22 ) Y = X X X (5) : Sep., 2009

39 5 2009 9 PERIODICAL OF OCEAN UNIVERSITY OF CHINA 39 (5) :908 912 Sep., 2009 Ξ, ΞΞ (, 266003) :,, ;;, : ; ; ; ; : Q142. 9 : A : 167225174 (2009) 052908205 [123 ], [425 ],, [122 ],,,, [6 ], [7 ] [8 ],, [3,9210 ] [9 ] [11 ], 1,, 10 20,,, 15 25 ;2 cm 19 20 ;5 15 cm 10 15 [6,12 ] 5. 68 13. 81 g 22. 3, 58. 75 g 15. 1 [13 ] An et al. (16,18,20,22 ) [15 ], 16,,SGR,,, SGR :16 ;22 [7 ] 50 g,10 20,20,, [14 ] 50 g, 10 20, [2 ],,,,,,, 15 2,18 2,21 2 3, [3 ], (15 2,18 2 ),(21 2 ) [14 ] 15, ( Y) ( X) : Y = 1. 612 62 + 0. 583 32 X - 0. 245 99 X 2 + 0. 023 41 X 3 Ξ ΞΞ : (2006BAD09A01) ; (30400333) :2009207207 ; :2009210209 : (19752),,,, E2mail :dongyw @ouc. edu. cn : E2mail :dongsl @ouc. edu. cn

5, : 909 (R2 = 0. 656 4, P < 0. 001), 1. 38 ; 18, ( Y) ( X) : Y = 1. 505 58 + 1. 3676 2 X - 0. 589 32 X 2 + 0. 058 89 X 3 (R2 = 1. 000, P < 0. 001), 1. 67,, An et al. (1), 15 3 18 3,C, [15 ] 1 [15 ] Fig. 1 Different temperature fluctuation modes [15 ], [14216 ],,,, (18 2 ), (18 4 ) (18 6 ),,, ( H K),( P K), (SOD) (CA T) SOD, CA T,, [17 ],,,,, [10 ] 2, 5 :,,, 9,,11, 12,,4, 6,7 8, 8, [18 ],, 5 7 ( ) 6 7 15 23,16 20 [18219 ] [20 ], 1 [21 ], (1 ) 13,,, 16,, 6,,,,, 18, 16 d ; 21 23, 10 12 d, 18 (8. 8 %) 23,,1. 3 %21 23, 36. 7 % 39. 0 % [12 ] 30 1 h, 13 d ( 20 ) 31. 4 %,68. 6 %,23. 1 %,11. 1 %, 18. 2 %, 47. 6 %,,18. 4 %,, 409. 2 62. 4 m,,

910 2 0 0 9 723. 4 67. 2 m, 3,, [22 ] Choe 4 :, ; [23 ], [24 ] 3. 1 Mitsukuri 7,,, [25226 ], (1),;,,,,, [28 ] ;,,, [14,28230 ] ;,< 1 mm26 40 d, 1. 5 %< 0. 2 % [8 ],, 1. 5 % 0. 5 % [8 ],, [31232 ] 1 Table 1 The threshold temperatures for aestivation in sea cucumber, A postichopus. japonicus Sites Body weight/ g Threshold temperature/ Reference Nanao Bay 20 Hokkaido, Miyagi, Aichi, Tokushima, Kagoshima 19 22-18 23 [27 ] Dalian, Sea area 20 24. 5 [17 ] Dalian, Ineoor 146. 7 5. 5 24. 5 25. 5 [14 ] 84. 0 3. 5 24. 5 25. 5 42. 5 2. 1 29. 5 30. 5, Penglai, Search area 25 85 24. 1 [28 ] 86 160 22. 9 > 160 21. 8, Qingdao, Sea area 20 [4 ] Qingdao, Indoor, [8,29,33234 ], 20 % 50 % Ji et al. 16 26, 5 g O 2 g - 1 h - 1 15 g O 2 g - 1 h - 1, 26 20 d,, 5 g O 2 g - 1 h - 1 [8 ],,,,, 20. 87 %, 79. 13 % [7,33 ], ( ), ;,, [35 ] 16 26, SOD CA T 25 37. 3 4. 1 26 [8 ],SOD CA T [8 ] (MPO) (L ZM), SOD CA T [22 ],,,, [36237 ], [38 ], ( ) Hsp,, Hsp, [8 ],. ().

5, : 911 3. 2,, [5,34 ] 268. 01 1. 77 g 36 d 71. 68 %, 4, [39 ],,(5 g) 30 34 1 h,1. 5 h 2 h, ( T L50 ) 32. 74,32. 37 30. 94 [9 ], (30 ) 2 h, 20 24 h 48 h 72 h, (34,2 h) 90 %,90 % 36. 7 %,, 2 d [9 ] (22 ) (12 ) 30 d,, T L50 (31. 8 ) (30. 9 ), [40 ] Hsp 30, Hsp70, 1 2 h,, 72 h Hsp70 [9 ],, Hsp70, hsp70 mrna [40 ] Hsp70 30 2 h, (20), Hsp, Hsp, [11 ] 5, : (1),,, ; (2),,,, ; (3),,,,,,,,, (,),, : [ 1 ],,,. [J ]., 2002, 33 (2) : 1822187. [2 ],,,. [J ]., 2005, 12 (1) : 33237. [ 3 ] Dong Y W, Dong S L, Tian X L, et al. Effects of diel temperature fluctuation on growth, oxygen consumption and proximate body com2 position in the sea cucumber A postichopus japonicus Selenka [ J ]. Aquaculture, 2006, 255 : 5142521. [ 4 ],. [J ]., 1999, 6 (3) : 1092110. [5 ],. [J ]., 2008, 23 (1) : 31236. [ 6 ] Yang H S, Yuan X T, Zhou Y, et al. Effects of body size and water temperature on food consumption and growth in the sea cucumber A2 postichopus japonicus ( Selenka) with special reference to aestivation [J ]. Aquaculture Research, 2005, 36 : 108521092. [ 7 ] An Z H, Dong Y W, Dong S L, 2007. Effects of high temperature and ration on the growth of juvenile sea cucumber japonicus (Selenka) [J ]. Aquaculture, 2007, 272 : 6442648. A postichopus [ 8 ] Ji T T, Dong Y W, Dong S L, Growth and physiological responses in the sea cucumber, A postichopus japonicus Selenka : aestivation and temperature [J ]. Aquaculture, 2008, 283, 1802187. [9 ] Dong Y W, Dong, S L, 2008. Induced thermotolerance and the ex2 pression of heat shock protein 70 in sea cucumber A postichopus japon2 icus Selenka [J ]. Fisheries Science, 74 : 5732578. [10 ] Dong Y W, Dong S L, Ji T T. Effect of different thermal regimes on growth and physiological performance of the sea cucumber A pos2 tichopus japonicus Selenka [J ]. Aquaculture, 2008, 275 : 3292334. [ 11 ] Dong Y W, Dong S L, Meng X L. Effects of thermal and osmotic stress on growth, osmoregulation and Hsp70 in sea cucumber ( A postichopus japonicus Selenka) [J ]. Aquaculture, 2008, 276 : 1792186. [ 12 ],,,. [ M ]. :, 2004. [ 13 ],. [J ]., 2004, 23 (7) : 16219. [ 14 ] Dong Y W, Dong S L. Growth and oxygen consumption of the juve2 nile sea cucumber A postichopus japonicus ( Selenka) at constant and fluctuating water temperature [ J ]. Aquaculture Research, 2006, 37 : 132721333.

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