11 3 Vol.11 No.3 2002 9 J0URNAL 0F SHANGHAI FISHERIES UNIVERSITY Sep. 2002!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! 1004-7271 2002 03-0283 - 06 Advances in the study of to0in in halobios 200090 WANG Yan ZH0U Pei-gen QI Xiao-yu College of Food Science Shanghai Fisheries University Shanghai 200090 China Key words toxin tetrodotoxin Conotoxin anthoplerin toxin S917 A 1000 1 Tetrodotoxin TTX Na + Na + TTX TTX Na + Na + 1 1.1 TTX TTX TTX 2002-01-18 1973-2001 Tel 021-65710346 E-mail ywang2@ shfu. edu. Cn
284 11 1 TTX 2 TTX TTX 3 4 5 TTX 6 7 TTX 1.2 1 2 1 TTX Na + Fig. 1 The structure formula of Tetrodotoxin TTX TTX 1 2 3- C-4 C-9 C-10 ph 1994 TTX TTX 3 TTX 2 Conotoxin CTX 10 ~ 30 α #! $ α-ctx AChR #-CTX Ca 2 +! -CTX Na + $-CTX Na + 1 ~ 2pmol 2.1 CTX α #-CTX NMR CD X- 2.1.1!-CT Gehrmann 4 α-ctx GI NMR α-ctx GI 2-7 3-13 5 11 310- Maslennikon 5 NMR α-ctx GI α-ctx GI
3 285 78% 22% Rogers 6 α-ctx IMI 2 loop Hu 7 X- α-ctx PnIB α-ctxpnia GI 2 PnIB 2 P2 1 2 1 2 1 Shon 8 HN - MR α-ctx MII 2 2 α-ctx #-CTX 14 AshCom 9 Fig. 2 Amino acid sequence ofα-ctx 6#-CTX α-ctx GI FGI N- Spin-Column FGI nachr K D 41. 3 士 8 2nmol / L Qniram 10 α- CTXIMI Asp5 Arg7 2.1.2!-CT Kim 11 #-CTX GVIA CD #-CTX MVIIA #-CTX MVIIC CD GVIA 2 CD 200nm Cotton CD CD Hirata 12 #-CTX GVIA MVIIA MVIIC Ca 2 + N Ca 2 + ATP Marian PriCe-Carter 13 #-CTX MVIIA Ca 2 + Lew 14 #-CTX GVIA Lys2 Tyr13 Ala 2 Argl7 Tyr22 Lys24 Ala < 100 GVIA N Ca 2 + Marian PriCe-Carter 15 #-CTX MVIIA MVIIA - Gly Gly 80% Pro-#-CTX MVIIA MVIIA - Gly MVIIA 10 Pro-#-MVIIA 100 ZiConotide #-CTX N ZiConotide 25 3 Fig.3 The space structure of Conotoxin by NMR I 3
286 11 3 Anthoplerin toxin AP ATX-2 47 4770 Fig. 4 4 The structure formula of Anthoplerin toxin 3.1 PDMS 2681.1 士 0.15 C 129 H 223 N 3 0 54 64 NaI0 4 4 Kishi 16 8 C 1 -C 7 C 8 -C 22 C 23 -C 37 C 38 -C 51 C 52 -C 75 C 76 -C 84 C 85 -C 98 C 99 -C 115 8 42 3.2 AP-A AP-B AP-A Na + AP-B Na + Kelso 17
3 287 Anthopleura xanthogrammica 6 5 Anmoniaa sulcata 47 I 49 RT-4B NIE-II5 PCR2-1047 Na + RT-4B NIE-II5 K 0.5 329nmol / L 1354nmol / L 49 Na + K 0.5 47nmol / L 108nmol / L 4 Jellyfish 18 Cephalotoxin 19 1000 ~ 10000 Tripheustes gratilla ph4.3 ~ 10.6 45.0 ~ 47.5 Lytechinus variegatus 0. 25mol / L Na0H l ~ 2h Lacticaudza semifisciate latictoxin I ~ V 20 90% dolastoxin Dolabella auricularia 1 ~ 9 3 Pro-Leu-Val- Gln - Thz- Gly -The 3 Gln -Thz Gln -ThZ P 388 B 15 3 microcystin cyanobacteria Microcyatis aeruginosa 50 MCdermott 21 -LR 30min 0.8! M 100! M DNA 5
288 11 1 Baden D G. Marine food-borne dinoflagellate toxins C. Review Cytology 1983 82-99. 2 Kao C Y. StruCture-aCtivity relations of tetrodotoxin saxitoxin and analogues C. Annals of the New York ACademy of science 1986 52. 3 Tambyah P A. Central-nervous-system effects of tetrodotoxin poisoning M. LanCet 1998 343 538-539. 4 Gehrmann J. StruCture determination of the three disulfide bond isomers of α-conotoxin GI a model for the role of disulfide bond in structural stability J. J Mol Biol 1998 278 401. 5 Maslennikov I V. Two distinct structures ofα-conotoxin GI in aqucous solution J. Eur J BioChem 1998 254 238. 6 Rogers J P. NMR solution structure ofα-conotoxin ImI and Comparison to other Conotoxins specific for neuronal nicotinic acetyleholine receptors J. BioChemistry 1999 38 13 3874. 7 Shu-Hong Hu. Crystal structure at a resolution ofα-conotoxin PnIB Comparison withα-conotoxin PnIA and GI J. BioChemistry 1997 36 38 11323. 8 Ki-Joon Shon. Three-dimensional solution structure of α-conotoxin MI anα3"2 neuronal nicotinic acetylcholine receptor-target edligand J. BioChemistry 1997 36 50 15693. 9 AshCom J D. CharaCterization ofα-conotoxin in teractions with nicotinic acetylcholine receptor and monoclonal antibodies J. Chem J 1997 328 245. 10 Quiram P A. CharaCterization ofα-conotoxin ImI essential for binding to neuronalα7 receptors J. The Journal of BiologiCal Chemistry 1998 273 18 11007. 11 Kim J. CirCular dichroism spectra of CalCium Channal antagonist #-Conotoxin J. BioChemiCal and biophysical 1997 203 1 133. 12 Hirata H. #-Conotoxins block neurotransmission in therat vas deferences by binding to gifferent presynaptic sites on the N-type Ca 2 + BioChemistry 1997 36 29 321. 13 PriCe-Carter M. Roles of individual disulfide bonds in the stability and folding of an#-conotoxin J. BioChemistry 1998 37 27 9851. 14 Lew M J. StruCture-funCtion relationships of #-Conotoxin GVIA J. The Journal of BiologiCal Chemistry 1997 272 18 12014. Channel J. 15 PriCe-Carter M. Folding of#-conotoxin influence of precursor sequences and protein disulfide isomerase J. BioChemistry 1996 35 48 15547. 16 Kishi R E. SCienCe M 1971. 172 495-498. 17 Kelso G J. IdentifiCation and CharaCterization of novel sodium Channel toxins from the sea anemone Anthopleura xanthogrammica M. Toxin 1998 36 1 41. 18 RiChard Benzinger. A specific interaction between the CardiaC sodium Channel and site-3 toxin anthopleurin B J. The Journal of BiologiCal Chemistry 1996 273 1 80. 19. J. 1999 18 1 43. 20. A. C 1998 58. 21 MCdermott C M. The CyanobaCterial toxin microcystin-lr CaninduCe apoptosis in a variety of Cell types M. Toxin 1998.36 12 1981.!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! 200090 BSA 16.1 0A 5.8 1.9