2158 2015 29 11 2158 ~ 2164 Journal of Nuclear Agricultural Sciences 1000-8551 2015 11-2158-07 9 1 2 1 3 4 2 1 471003 2 / 100101 3 451003 4 471003 9 8 9 32. 75% ~ 40. 64% 40. 58% ± 0. 08% 9 4 K > Ca > Mg > Na K Ca Mg Na 9 4 Zn > Fe > Mn > Cu Fe Mn Cu Zn DOI 10. 11869 /j. issn. 100-8551. 2015. 11. 2158 Paeonia suffruticosa 10 503 15 β 1-39. 3% 15-16 7. 89% 17 2-3 18-20 4-7 21 Q 92% α - 42% 8-13 9 14 2015-04-22 2015-08-18 U1304308 122102110110 122102310562 09001446 E-mail hechunling68@ 126. com E-mail hkdhxg@ 126. com
11 9 2159 Na 589. 592 nm Ca 317. 933 nm Mn 257. 610 nm Fe 259. 940 1 nm Cu 324. 754 nm Zn 206. 200 nm K 766. 491nm Mg 279. 553 nm 1. 1 X = A - K 25 M X 9 mg kg - 1 A mg L - 1 K mg L - 1 M g 1. 3 16cm 10cm Microsoft Excel SPSS Base Ver. 16. 0 50 ~ 60 DZF6050 120 h 2 60 4 2. 1 1. 2 9 1. 2. 1 GB 5009. 5-2010 22 9 1. 0000g ± 0. 0001 g 32. 75% ~ 40. 64% 0. 5 g CuSO 4 4. 5g K 2 SO 4 40. 58% ± 0. 08% 10 ml 39. 49% ± 0. 21% Tecator Digesror Auto 32. 78% ± 0. 04% 1 240 30 min 430 1. 5 h 20 min 9 Kjeltec 8200 8 2 1. 2. 2 8 Prodigy ICP 23 1 1. 0000g ± 2. 2 0. 0001 g 100 ml 2. 2. 1 9 K 15 ml = 9 1v v Ca Mg Na 4 9 4 K > Ca > Mg > Na K 14 828. 91 ~ 19 672. 68 mg kg - 1 25 ml 17 952. 59 ± 1 456. 63mg kg - 1 Ca 1 591. 79 ~ 5 708. 89 mg kg - 1 3 760. 13 ± 1. 0 kw 0. 6 mpa 3 668. 23 mg kg - 1 2 937. 01 ± 309. 25 15 L min - 1 0. 2 L min - 1 mg kg - 1 Na 51. 83 ~ 186. 40 mg kg - 1 374. 06 Pa 1. 5 ml min - 1 84. 54 ± 33. 86mg kg - 1 1% 2 1 251. 85 mg kg - 1 Mg 2 418. 75 ~ K Ca Mg Na
2160 29 1 Table 1 The protein content in pollen of nine Paeonia suffrutisosa cultivars /% The protein content The protein content Xue ying tao hua 37. 26 ± 1. 61c Lu he hong Luo yang hong 36. 41 ± 0. 25c Ying hong Rou fu rong 39. 49 ± 0. 21ab Feng dan bai Zi yan zheng hui 38. 29 ± 0. 14bc Cao zhou hong 38. 19 ± 0. 01bc 32. 78 ± 0. 04e 37. 21 ± 0. 79c 34. 21 ± 0. 03d Dao jin 40. 58 ± 0. 08a P < 0. 05 Note Different lowercases letters indicate significant difference among the different cultivars at 0. 05 level. The same as following. 2 Table 2 The constant elements content in pollen of nine Paeonia suffrutisosa cultivars / mg kg - 1 Constant elements K Ca Mg Na Xue ying tao hua 18 840. 12 ± 248. 01b 4 634. 82 ± 383. 58bc 2 821. 34 ± 114. 37c 70. 67 ± 9. 42c Luo yang hong 18 794. 40 ± 886. 50b 4 099. 31 ± 30. 71c 3 163. 72 ± 33. 06b 85. 54 ± 3. 78bc Rou fu rong 18 172. 26 ± 101. 83bc 2 607. 95 ± 306. 83e 2 833. 04 ± 37. 94c 90. 43 ± 4. 28bc Zi yan zheng hui 19 320. 05 ± 49. 07ab 4 243. 62 ± 64. 72c 3 026. 15 ± 57. 75bc 57. 39 ± 3. 49c Dao jin 14 918. 43 ± 125. 90d 1 616. 91 ± 35. 52f 2 435. 41 ± 23. 58d 61. 79 ± 1. 26c Lu he hong 17 096. 28 ± 112. 51c 3 519. 83 ± 95. 23d 2 836. 90 ± 28. 82c 142. 21 ± 4. 86a Ying hong 19 640. 29 ± 45. 80a 5 514. 56 ± 27. 49a 3 533. 13 ± 191. 11a 74. 64 ± 5. 37bc Feng dan bai 17 120. 19 ± 628. 35c 2 715. 14 ± 125. 78e 2 717. 84 ± 63. 53c 66. 38 ± 2. 92c Cao zhou hong 17 671. 27 ± 124. 26c 4 889. 08 ± 83. 32b 3 065. 56 ± 120. 16b 105. 20 ± 25. 69b 4 K Ca Mg Na Fe Cu Zn Mn 3 2 2. 2. 2 9 4 Fe Cu Zn Mn 4 Zn Cu 9 4 0. 919 Ca K Mg 0. 725 Zn > Fe > Mn > Cu Zn 99. 58 ~ 0. 653 Zn Mn 0. 667 165. 29 mg kg - 1 120. 51 ± 17. 91mg kg - 1 Fe 2. 3 53. 67 ~ 257. 13 mg kg - 1 95. 17 ± 36. 68mg kg - 1 Cu 13. 38 ~ 30. 60 mg kg - 1 22. 03 ± 5. 27mg kg - 1 Mn 9 3 32. 96 ~ 59. 71mg kg - 1 44. 47 ± 7. 40mg kg - 1 2. 2. 3 8 8 9 Q 1 Cu Zn Fe Mn 3
11 9 2161 3 Table 3 The microelement content in pollen of nine Paeonia suffrutisosa cultivars / mg kg - 1 Microelement Fe Cu Zn Mn Xue ying tao hua 81. 47 ± 1. 30bc 25. 04 ± 0. 40c 119. 63 ± 3. 42cd 34. 11 ± 0. 90f Luo yang hong 55. 10 ± 1. 53c 17. 35 ± 0. 11e 101. 49 ± 2. 01e 40. 03 ± 0. 26e Rou fu rong 63. 74 ± 9. 86c 24. 69 ± 0. 35c 115. 36 ± 5. 07d 48. 02 ± 1. 26c Zi yan zheng hui 67. 74 ± 4. 21bc 27. 37 ± 0. 58b 116. 97 ± 1. 28cd 38. 82 ± 0. 12e Dao jin 91. 41 ± 10. 01bc 19. 97 ± 0. 41d 105. 68 ± 4. 03e 53. 76 ± 0. 72b Lu he hong 99. 51 ± 1. 74bc 13. 41 ± 0. 03f 137. 15 ± 13. 02b 47. 72 ± 0. 48c Ying hong 117. 83 ± 4. 32b 20. 38 ± 0. 57d 108. 21 ± 4. 62de 37. 97 ± 1. 04e Feng dan bai 176. 54 ± 92. 20a 19. 72 ± 0. 41d 120. 68 ± 4. 66c 55. 74 ± 0. 26a Cao zhou hong 103. 19 ± 2. 47bc 30. 35 ± 0. 27a 159. 45 ± 4. 52a 44. 05 ± 0. 42d Table 4 4 Correlation matrix for the mineral elements content in Paeonia suffrutisosa pollens Mineral elements K Ca Mg Na Fe Mn Zn Cu K 1. 000 Ca 0. 725* 1. 000 Mg 0. 594 0. 653* 1. 000 Na - 0. 165 0. 054 0. 058 1. 000 Fe 0. 209 0. 053 0. 130 0. 073 1. 000 Mn - 0. 588-0. 444-0. 048 0. 219-0. 169 1. 000 Zn - 0. 212 0. 015-0. 176 0. 248-0. 059 0. 667* 1. 000 Cu - 0. 199-0. 104-0. 338-0. 048-0. 176 0. 597 0. 919** 1. 000 * Note * ** showed significant correlation **showed highly significant correlation. 3 24% 5% 16 13% 3 28 3% 3. 1 10 29 16% ~ 19% 16 24 9 30-7% ~ 40% 31 25 39. 3% 16 6. 125% Bombus spp. 26 - ~ 10. 238% 3 27 32
2162 29 XYTH LYH RFR ZYZH DJ LHH YH FDB CZH Note XYTH Xue ying tao hua LYH Luo yang hong RFR Rou fu rong ZYZH Zi yan zheng hui Fig. 1 DJ Dao jin LHH Lu he hong YH Ying hong FDB Feng dan bai CZH Cao zhou hong. 1 Dendrogram of cluster analysis in pollen of nine Paeonia suffrutisosa cultivars 33 37 3. 2 8 Q 9 3 25 34 34 ICP - MS Ca Na 156. 4 145. 9 mg kg - 1 Fe K Zn Mg 20 ~ 60 mg kg - 1 Cu 0. 73 mg kg - 1 35 3 3 4 Ca Fe Mn Zn Cu Ca 858. 1 mg kg - 1 9 8 Cu Fe Zn Mn 54. 53 19. 16 29. 98 38 3. 71 μg g - 1 36 9 8 K Ca 32% Mg Na 4 Fe Cu Zn Mn 4 K Ca Mg Fe Mn Q
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2164 Journal of Nuclear Agricultural Sciences 2015 29 11 2158 ~ 2164 Analysis of the Protein and Mineral Elements Content in Pollen of Nine Paeonia suffrutisosa s HE Chunling 1 2 XU Shanshan 1 ZHANG Shuxia 3 HOU Xiaogai 4 ZHU Chaodong 2 1 College of Forestry Henan University of Science and Technology Luoyang Henan 471003 2 Key Laboratory of Zoological Systematics and Evolution /Institute of Zoology Chinese Academy of Sciences Beijing 100101 3 Henan Entry-exit Inspection and Quarantine Bureau of P. R. China Zhengzhou Henan 451003 4 College of Agriculture Henan University of Science and Technology Luoyang Henan 471003 Abstract In this study the protein content and eight kinds of mineral elements content in pollen of nine Paeonia suffrutisosa cultivars were respectively measured by using kjeldahl nitrogen method and ICP-AES Inductively Coupled Plasma Atomic Emission Spectrometry to reveal the differences of the nutritional value in different cultivars. The differences of these indexes among these cultivars were also analyzed. The results suggest that there have abundant protein and mineral elements in the pollen of Paeonia suffrutisosa and the obvious differences of these indexes are existed among different cultivars of Paeonia suffrutisosa. The contents of protein are 32. 75% to 40. 64% and the contents of protein in the Paeonia suffruticosa cv. Dao jin pollen 40. 58% ± 0. 08% is the highest. The measurement results of four major elements of K Ca Mg and Na show that the maximum concentration of element is K the next are Ca and Na and the minimum is Na. Among these cultivars the pollens from Paeonia suffruticosa cv. Ying hong have the highest contents of K Ca and Mg. The pollens from Paeonia suffruticosa cv. Lu he hong have the highest contents of Na. The measurement results of four trace elements of Zn Fe Mn and Cu show that the highest concentration of element is Zn the next are Fe and Mn the lowest is Cu. Among these cultivars the pollens from Paeonia suffruticosa cv. Feng dan bai have the highest contents of Fe and Mn. The pollens from Paeonia suffruticosa cv. Cao zhou hong have the highest contents of Cu and Zn. This study have great significance to further research and develop the products of Paeonia suffrutisosa pollen. Keywords Paeonia suffrutisosa pollen protein mineral elements