38 1 2014 2 Journal of Nanchang UniversityNatural Science Vol. 38 No. 1 Feb. 2014 1006-0464201401-0086-05 1 2 1 1 1* 1. 330047 2. 225009 2 c9 t11-cla t10 c12-cla 3T3-L1 5 10 20 μmol L - 1 c9 t11-cla t10 c12-cla CON MOD ROS c9 t11-cla t10 c12-cla t10 c12-cla 20 μmol L - 1 t10 c12-cla ROS P > 0. 05 P < 0. 05 t10 c12-cla 3T3-L1 3T3-L1 Q493. 4 DOI:10.13764/j.cnki.ncdl.2014.01.018 A Effects of conjugated linoleic acid isomers on glucose consumption of insulin-resistant adipocytes LIU Xiaohua 1 LIU Ping 2 LI Haixing 1 LI Chaobo 1 CAO Yusheng 1 * 1. Sino-German Joint Research Institute Nanchang University Nanchang 33007 China 2. The First People s Hospital of Yangzhou Yangzhou 225009 China AbstractTwo main Conjugated Linoleic Acids CLAsc9 t11 and t10 c12 in natural food were selected to investigate their effects on enhancing insulin sensitivity. The insulin-resistant 3T3-L1 adipocytes were induced by high concentration insulin which was used as cell model. Glucose consumption was tested each 24 h for all treatments. It was found that c9 t11-cla did not have any activity in enhancing insulin sensitivity. However t10 c12-cla showed significant ability to enhance glucose consumption in a concentration and time dependent manner. For instance at the concentration of 20 μmol L - 1 t10 c12-cla had the same effect as rosiglitazonep > 0. 05 which was a commonly used medicine in type-2 diabetes. The content of lipid in the cells was significantly decreased by the treatment of t10 c12-cla P < 0. 05which might be associated with the activity of enhancing insulin sensitivity. Key wordsconjugated linoleic acidisomersinsulin resistance3t3-l1 adipocytebioactivity 2 90% Ⅱ 3 800 Ⅱ 2020 3 1 2013-07-15 31260373 GJJ12019 1974 - * 1945 - E-mailyyssccc@ hotmail. com
1 87 Ⅱ 1. 3. 2 CON MOD TZD ROS 5 μmol L - 1 5 10 20 μmol L - 1 c9 t11-cla CLA15 CLA110 2 CLA120 5 10 20 μmol L - 1 t10 c12-cla CLA2 5 CLA2 10CLA2 20 ROS 3 CLA1 CLA2 24 48 72 h 1 1. 3. 3 MTT MTT PBS conjugated linoleic acid CLA 5 mg ml - 1 20μL CLA MTT 4 h 100 μl DMSO c9 t11-cla t10 c12-cla 490 nm CLA 4 1. 3. 4 CLA 5-2 6 CLA 1. 3. 5 24 h 7 CLA 10-9 mol L - 1 8-10 CLA DMEM 30 min 10% DMEM 24 h 3T3-L1 10% DMEM CLA 24 h CLA 1. 3. 6 24 48 h CLA 0. 5% O PBS O 10 1 min 490 nm 1. 1 3T3-L1 SPSS 13. 0 DMEM Solarbio -EDTA Solarbio 2 Sigma DMSO Amresco 2. 1 CLA MTT Amresco c9 t11-cla t10 c12-cla O Solarbio c9 t11-cla t10 c12-cla 75. 4 μmol L - 1 CLA 3T3-L1 12 0. 4% CLA 1. 2 Thermo Scientific 4 g d - 17 CLA Labsystem Olympus CLA CLA A&D CLA 1. 3 1. 3. 1 3T3-L1 3T3-L1 11 1. 3. 7 3 x ± s 13 CLA 2
88 2014 3T3-L1 1 CLA MTT 48 h 3T3-L1 MOD ROS c9 t11-cla t10 c12-cla 5 10 20 μmol L - 1 3 P > 0. 05 1 CLA MTT 1 0.40 0.35 0.30 0.25 0.20 0.15 0.10 COD ROS CLA1(5) CLA1(10)CLA1(20) CLA2(5) CLA2(10) CLA2(20) /mmol L - 1 24 h 48 h 72 h CON 3. 03 ± 0. 14 aa 3. 10 ± 0. 18 aa 3. 03 ± 0. 26 aa MOD 2. 15 ± 0. 15 ad 2. 21 ± 0. 07 ad 2. 54 ± 0. 08 bb ROS 2. 58 ± 0. 21 ab 2. 82 ± 0. 15 abb 2. 91 ± 0. 09 ba CLA15 2. 22 ± 0. 06 acd 2. 22 ± 0. 13 ad 2. 57 ± 0. 08 bb CLA110 2. 18 ± 0. 14 acd 2. 22 ± 0. 05 ad 2. 53 ± 0. 10 bb CLA120 2. 19 ± 0. 10 acd 2. 27 ± 0. 17 ad 2. 57 ± 0. 08 bb CLA25 2. 27 ± 0. 11 acd 2. 42 ± 0. 07 abcd 2. 63 ± 0. 16 bb CLA210 2. 41 ± 0. 08 ac 2. 56 ± 0. 05 abc 2. 64 ± 0. 08 bb CLA220 2. 64 ± 0. 07 ab 2. 87 ± 0. 07 bb 2. 93 ± 0. 11 ba p < 0. 05 p < 0. 05 CLA1c9 t11-cla CLA2t10 c12-cla 2 5 μmol L - 1 3T3-L1 ROS 2. 2 CLA MODP < 0. 05 1 3T3-L1 1 CLA220 ROS P CON > 0. 05 t10 c12-cla 20 μmol L - 1 48 h MOD P < 0. 05 ROS MOD Li P < 0. 05 1 c9 t11-cla t10 c12-cla CLA MOD CLA1 5 CLA1 c9 t11-cla t10 c12-cla 10CLA120 24 ~ 72 h 14 Stringer P > 0. 05 c9 t11-cla t10 c12-cla 3T3-L1 13 CLA25 P > 0. 05 10 20 μmol L - 1 MODP < 3T3-L1 0. 05 1 t10 c12-cla t10 c12-cla 10 μmol L - 1 15-17 20 μmol L - 1 P < 0. 05CLA220 24 48 3T3-L1 h P < 0. 05 48 h 11 1 MOD 48 h t10 c12-cla P > 0. 05 72 h P < 0. 05
1 89 2. 3 OD 24 h CON 0. 705 ± 0. 082 AB 0. 701 ± 0. 102 A t10 c12- MOD 0. 763 ± 0. 038 A 0. 752 ± 0. 050 A CLA ROS 2 ROS 0. 750 ± 0. 040 A 0. 718 ± 0. 047 A CLA15 0. 755 ± 0. 046 A 0. 756 ± 0. 053 A CLA110 0. 753 ± 0. 044 A 0. 749 ± 0. 046 A CLA120 0. 752 ± 0. 056 A 0. 751 ± 0. 047 A CLA25 0. 740 ± 0. 040 AB 0. 711 ± 0. 023 A CLA210 0. 717 ± 0. 030 AB 0. 651 ± 0. 038 AB CLA220 0. 652 ± 0. 049 B 0. 569 ± 0. 057 B t10 c12-cla 10 t10 c12- CLA 13 18 2 /mmol L - 1 CON 3. 10 ± 0. 19 A 3. 35 ± 0. 07 A MOD 2. 19 ± 0. 10 D 2. 32 ± 0. 23 D ROS 2. 84 ± 0. 13 B 3. 05 ± 0. 09 B CLA25 2. 36 ± 0. 07 CD 2. 65 ± 0. 15 C CLA210 2. 52 ± 0. 09 C 2. 80 ± 0. 11 C CLA220 2. 86 ± 0. 11 B 3. 08 ± 0. 11 B CLA 2. 4 CLA t10 c12-cla 3T3-L1 2 t10 c12-cla 19-20 20 μmol L - 1 t10 c12-cla 2 t10 c12-cla 3T3-L1 CLA CON MOD ROS CLA1 P > 0. 05 CLA2 20 P < 0. 05 3 1MUOIO D M NEWGARD C B. Molecular and metabolic t10 c12-cla mechanisms of insulin resistance and β-cell failure in type t10 c12-cla 2 diabetes J. Molecular Cell Biology 2008 9193-205. Metabolism 2009 114 275-84. 3SHIH C C LIN C HWU J B. Eriobotrya japonica improves hyperlipidemia and reverses insulin resistance in Glu4 high-fat-fed micej. Phytotherapy Research 2010 24 21 CLA 12 1769-1780. t10 c12-cla 3T3-L1 4. J. 2007 312 163-165. 3 CLA 3 24 h 48 h 3T3-L1 c9 t11-cla t10 c12-cla 3T3-L1 2GREY A. Thiazolidinedione-induced skeletal fragilitymechanisms and implicationsj. Diabetes Obesity and
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