Study of the gene expression profile in the bone tissue with kidney yin deficiency syndromes in primary oste oporosis

203 2 9 2 Chin J OsteoporosDecember 203 Vol 9No. 2 Published online www. wanfangdate. com. cn doi0. 3969 /j. issn. 006-708. 203. 2. 00 25 2 2 *. 350003 2. 350007 R68 A 006-7082032-25-05 3 3 3 3 4 2378 4026 407 3 344 Study of the gene expression profile in the bone tissue with kidney yin deficiency syndromes in primary oste oporosis LI Shengqiang FENG Eryou 2 ZHANG Yiyuan 2 QIU Longlong XIE Bingying XIE Lihua GE Jirong. Institute of Basic Medical ScienceFujian Institute of Traditional Chinese MedicineFuzhou 350003 2. Department of Joint Surgery the Second Hospital of Fuzhou Affiliated to Xiamen UniversityFuzhou 350007China Corresponding authorge JirongEmailgjrrjgcy@ sohu. com AbstractObjective To investigate the characteristics of genes expression profiles of kidney yin deficiency in primary osteoporosis by analyzing the difference of gene expression profiles in the bone tissues in patients with primary osteoporosis. Methods Osteoporotic patients were randomly selected. All the patients were divided into kidney yin deficiency groupkidney yang deficiency groupand non-kidney deficiency group according the syndrome differentiation of traditional Chinese medicine. Each group had 3 patients. Another 3 people with normal bone mineral density BMD were selected as normal control. Gene expression profiles of the bone tissues in all 4 groups were detected using the human genome-wide expression profile chip. The differentiated expression genes were screenedand the analysis of the related functions of these genes including the pathways was conducted. Results Compared with that in kidney yang deficiency groupnon-kidney deficiency groupand normal control group the number of differentiated expression genes in kidney yin deficiency group was 23784026and 407respectively. Compared with that in these 3 groupsthe number of common differentiated expression gene in kidney yin deficiency group was 344. These genes were involved in pathways including immunoregulationmineral absorptionhormone synthesisand histidine metabolism. Conclusion Related genes of kidney yin deficiency syndromes in primary osteoporosis are mainly related to the signal pathways such as immunoregulationhormone synthesishistidine metabolism and mineral absorption. Key wordsprimary osteoporosiskidney yin deficiency syndromegene expression profile Osteoporosis OP 873280 20R038-5 20-CX-30 200--3 * Emailgjrrjgcy@ sohu. com

26 203 2 9 2 Chin J OsteoporosDecember 203 Vol 9No. 2 RNA. 5 RNA cy3-utp crnas RNEASY Mini Kit Ambion RNA 3 Fragmentation Reagents crnas Agilent 4 44K 65 7 h Agilent DNA Microarray. Scanner. 6 3 3 3 2. 0 0. 5 3 2 NCBI OMIM DAVID KEGG 52 ~ 68 62. 35 ± 4. 64. 7 SPSS 3. 0 2 P < 0. 05 2 986 2 3 3 2. 2 3 4 2378 426. 2 X Hologic Discovery 952 4026 2354 672 WITrizol Invitrogen RNEASY Mini Kit QIAGEN 4 44K 20 96 Agilent Nanodrop ND-000 Thermo scientific Agilent Agilent DNA Microarray Scanner Agilent. 3 Hologic Discovery WI X 2. 2 3 < % L 2-4 GO Ward s g /cm 2 407 3 344 286 58 344 GO. 4 RNA 22 g Trizol RNA Nanodrop ND- 000 260 nm 280 nm A RNA l%

203 2 9 2 Chin J OsteoporosDecember 203 Vol 9No. 2 27 Table 3 Differentiated genes in the bone tissue with kidney-yin deficiency syndromes in primary osteoporosis compared with the genes in the bone tissues in the other 3 groups. GeneSymbol Gene bank Accession Regulation P Fold Change P value CCL4L CCL8 LY9 PRSS6 PTGR LILRA6 FCGBP MS4A2 NCR3 IL-32 NM_0002633 TRPM3 NM_0000747 TRPM7 NM_07672 KCNK2 NM_0007424 COL4A6 NM_03364 KCNN4 NM_002250 SLC25A27 NM_004277 SLC4A NM_0046037 SLC47A NM_08242 SLC39A0 NM_020342 CNN3 NECAB3 LIMCH CAMKK2 BMPR2 BBX BBS9 THBS4 CLDN23 COL9A3 ITGAX MYO5B FBLN STAT3 IL6ST TNFSF3B PTP4A OR3A3 WNT4 FFAR3 ADAM7 EPHB ADAM7 FOXF FOXG ZNF58A ARNT GATA6 ATXN3 ZNF65 LASS3 FOSB KIAA967 ZBTB6 CUL2 UTPL PDCD7 OLR TXNDC5 AY766452 NM_005623 NM_00033667 NM_005865 NM_0222 NM_02438 NM_003890 NM_00039 NM_4730 NM_00839 NM_03232 NM_04988 NM_7225 NM_00204 NM_020235 NM_0445 NM_003248 NM_94284 NM_00853 NM_000887 NM_00080467 NM_00996 NM_23662 NM_00284 NM_006573 NM_003463 NM_02373 NM_03076 NM_005304 NM_00383 NM_00444 NM_00383 NM_0045 NM_005249 NM_04803 NM_00668 NM_005257 NM_004993 NM_98480 NM_78842 NM_006732 NM_0274 NM_006006 NM_00359 NM_06037 NM_005707 NM_002543 NM_03080 4. 75 9. 07 2. 05 7. 06 2. 45 4. 30 4. 2 3. 59 3. 52 2. 6 3. 02 7. 59 2. 8 9. 20 2. 55 7. 34 3. 36 4. 7 2. 08 5. 4 2. 00 3. 35 6. 89 2. 77 2. 33 2. 23 4. 85 3. 98 3. 69 5. 03 2. 09 3. 0 5. 29 3. 02 2. 42 7. 49 2. 6 2. 8 2. 48 2. 22 3. 7 2. 22 2. 7 25. 63 3. 97. 85 2. 90 5. 52 3. 07 2. 96 49. 90 5. 20 4. 54 4. 3 8. 00 9. 67 2. 2 3. 75 8. 05944E-05 0. 0006 0. 0002 4. 44967E-06 7. 58355E-05 0. 025 4. 67986E-06 0. 000 5. 3669E-05 0. 026 9. 80837E-05 0. 09 0. 003 0. 02 0. 004 0. 022 0. 02 0. 0007 0. 0 0. 000 5. 02075E-06 0. 0002 4. 66869E-05 0. 005 0. 003 0. 0009 2. 233E-06 0. 0 0. 04 0. 0007 0. 04 0. 000 0. 0005 0. 0008 0. 006 0. 0 0. 0004 3. 0072E-05 0. 0004 0. 04 0. 008 9. 6735E-05 0. 02 2. 20753E-05

28 203 2 9 2 Chin J OsteoporosDecember 203 Vol 9No. 2 2. 3 Pathway -3 0 pathway 3. 2 TRPM3 NF-kappa B TRPM7 KCNK2 COL4A6 KCNN4 SLC25A27 3 TRPM TRPM2 TRPM3 TRPM8 Ca 2 + TRPM6 TRPM7 4 Mg 2 + 4 5 TRPM3 TRPM7 KCNN4 2 6 5 KCNN4 RNA 3. 3 CNN3 NECAB3 LIMCH CAMKK2 FOSB BMPR2 BBX BBS9 THBS4 CLDN23 COL9A3 ITGAX MYO5B FBLN 4 CNN3 Calponin 7-8 CNN CNN 6 CNN3 3. 7 CNN3 IL-32 CCL4L CCL8 LY9 PRSS6 PTGR LILRA6 FCGBP MS4A2 NCR3 IL-32 0 IL-32 CCL4L CCL4 CCR5 HIV CCL8 CC T NK 9 LY9 CD229 SLAM SAP 0 SLC4A SLC47A SLC39A0 9 TRPM TRP TRPM-TRPM8 8 IL-32 253

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