The Recent Progress in Transgenic Animal Research and Its Applications

2010 32 5 0847-0854 http / /xuebao. jxau. edu. cn Acta Agriculturae Universitatis Jiangxiensis E - mail ndxb7775@ sina. com * 100193 S813. 1 A 1000-2286 2010 05-0847 - 08 The Recent Progress in Transgenic Animal Research and Its Applications LIU Shen LI Ning * State Key Laboratory for Agrobiotechnology China Agricultural University Beijing 100193 China Abstract This paper reviews the recent advances and applications of transgenesis in livestock and their derivative products. Firstly the developments in transgenic techniques are presented. Secondly its emerging applications in the field of agriculture pharmaceuticals environmental protection biological materials and anti - terrorism are analyzed. Finally the prospects of transgenic animals are discussed. Key words transgenic technology transgenic animals transgenic breeding pharmaceuticals animal models for human disease 20 80 1976 Jaenisch 1 2 1980 SV40 TK pbr322 2010-09 - 24 2009ZX08010-014B 863 2009AA10Z110 1984 E-mail liushen163@ 163. com * 1962 973 5 PigBioDivII 1978 9 1982 7 1985 9 1991 7 1989 1 1990 7 3 6 WCGALP 260 SCI 100 Nature PNAS Journal of Virology Genetics Genomics BMC Genomics BMC Molecular Biology Molecular Phylogenetics and Evolution 21 Genomics and Biotechnology in Livestock Breeding 11 23 E-mail ninglbau@ public3. bta. net. cn

848 32 78 1982 Palmiter 3 - Ⅰ 2 4 1% ~ 2% 1% ~ 5% 1997 Wilmut 5 Dolly 100% Ⅸ Polly 6 2000 McGreath 7 α1 COL1A1 β - α1 - AAT 650 μg /ml AAT 18 μg /ml 100% 1985 8 ES ES ES 2 9 ES ES 2006 Yamanaka 10 ips 3 ips ES ips 1 1. 1 DNA 1989 Lavitrano 11 CAT Lavitrano DNA 1. 2 2002 Lois pfugw PΔ8. 9 pvsv - G 293T 86% 90% 12 8. 5 kb 1. 3 Sleeping Beauty SB PiggyBac PB SB 1000

5 849 1997 Ivics 13 DNA PiggyBac 2005 14 1. 4 RNA RNA RNAi 19 ~ 23 nt RNA mrna sirna 90% sirna shrna 15 shrna PERV RNAi ZFN DNA N DNA Cys2 - His2 C FokI FokI DNA DNA DNA 16 10-6 ~ 10-7 10-1 ~ 10-2 Sigma 2 4 1 2 3 4 2. 1 GH 1985 Harmmer 4 17% Pursel 17 2 11% ~ 14% 16% ~ 18% Du 18 2 ~ 6 AquaBounty Myostatin C 30% 2 ~ 3 86% 19 Myostain 4 Myostatin Ⅲ 11 bp 14 Myostatin 2 Myostatin Myostatin

850 32 2. 2 Mx1 Garber 20 Mx1 1992 Berm Visna Visna 1994 Clements 21 Visna Eve 2000 Kerr 22 2005 Donovan 14% 71% 2006 Maga 23 270 mg /L TSE PRNP PRNP 24 PRNP 2001 Denning 25 4 PRNP 2004 Kuroiwa 26 PRNP 2007 Richt 27 PRNP Golding 28 RNA PRNP 2. 3 2004 Saeki K 29 Δ12 6 20% 2004 Kang 30 fat - 1 2006 31 fat - 1 fat - 1 ω - 6 ω - 3 2. 4 1996 Damak 32 IGF - 1 6. 2% 1998 Bawden 33 Ⅱ 2005 Amdas 34 2. 5 70% β - α - 2003 Brophy 35 β - κ - 20% 100% 2002 36 α -

5 851 2 ~ 3. 43 g /L α - 1. 55 g /L β - 3 3. 1 2006 8 Ⅲ ATryn Amy Brock 2013 1 600 370 37 1 β - γ - 2 8 000 ~ 10 000 L 1 ~ 10 g /L 3 4 2002 Harvey 38 β - 2007 IFN - β - 1a mir24 39 3. 2 2008 Roger 40 CFTR - / - CFTR - / - 2009 Kragh 41 7 Mehtali HIV - 1 - Tat Okuma - 4 HIV - 1 400 310 40 3. 3 Rosengard

852 32 hdaf 27 hdaf 42 Dai α - 1 3 43 - α - 1 3 44 α - 1 3 α - 1 3 4 2003 NEXIA 4 ~ 5 45 5 75% 46 6 Q PROTEXIA 2007 Huang 47 0. 1 ~ 5 g /L 3 PA LF EF Valortim PA SparVax TM PharmAthene 3 7 1980 1 2

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